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http://dx.doi.org/10.20307/nps.2018.24.2.103

Optimization of Extraction Condition of Methyl Jasmonate-treated Wild Ginseng Adventitious Root Cultures using Response Surface Methodology  

Liu, Qing (College of Pharmacy, Chungbuk National University)
Jo, Yang Hee (College of Pharmacy, Chungbuk National University)
Ahn, Jong Hoon (College of Pharmacy, Chungbuk National University)
Kim, Seon Beom (College of Pharmacy, Chungbuk National University)
Paek, Kee-Yoeup (Department of Horticultural Science, Chungbuk National University)
Hwang, Bang Yeon (College of Pharmacy, Chungbuk National University)
Park, So-Young (Department of Horticultural Science, Chungbuk National University)
Lee, Mi Kyeong (College of Pharmacy, Chungbuk National University)
Publication Information
Natural Product Sciences / v.24, no.2, 2018 , pp. 103-108 More about this Journal
Abstract
The usage of wild ginseng (Panax ginseng C.A. Meyer) has been limited due to short supply and high price. Therefore, sufficient production as well as efficient extraction of mountain ginseng are required for the development as products. In this study, wild ginseng adventitious root cultures were prepared for efficient production with advantages of fast growth and stable production. Treatment of methyl jasmonate (MJ) to wild ginseng adventitious root cultures increased the extraction yield and antioxidative activity. Further investigation on effect of extraction conditions suggested the importance of ethanol concentration on antioxidative activity and extraction yield of MJ-treated wild ginseng adventitious root cultures. Optimized extraction condition of MJ-treated wild ginseng adventitious root cultures for maximum extraction yield and antioxidative activity was determined using response surface methodology with three-level-three-factor Box-Behnken design (BBD). Extraction of 1 g MJ-treated wild ginseng adventitious root culture with 30 ml of 9% ethanol at $30^{\circ}C$ produced 310.2 mg extract with 71.0% antioxidative activity at $100{\mu}g/ml$. Taken together, MJ-treated wild ginseng adventitious root culture is valuable source for wild ginseng usage and optimized extraction condition can be used for the development of functional products or folk remedies.
Keywords
Wild ginseng adventitious root cultures; Methyl jasmonate; Optimized extraction condition; Antioxidative activity; Extraction yield;
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