Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Complete 1H-NMR and 13C-NMR spectral assignment of five malonyl ginsenosides from the fresh flower buds of Panax ginseng

  • Wang, Yu-Shuai (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences) ;
  • Jin, Yin-Ping (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences) ;
  • Gao, Wei (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences) ;
  • Xiao, Sheng-Yuan (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences) ;
  • Zhang, Yu-Wei (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences) ;
  • Zheng, Pei-He (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences) ;
  • Wang, Jia (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences) ;
  • Liu, Jun-Xia (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences) ;
  • Sun, Cheng-He (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences) ;
  • Wang, Ying-Ping (Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences)
  • Received : 2015.03.03
  • Accepted : 2015.08.06
  • Published : 2016.07.15
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Abstract

Background: Ginsenosides are the major effective ingredients responsible for the pharmacological effects of ginseng. Malonyl ginsenosides are natural ginsenosides that contain a malonyl group attached to a glucose unit of the corresponding neutral ginsenosides. Methods: Medium-pressure liquid chromatography and semipreparative high-performance liquid chromatography were used to isolate purified compounds and their structures determined by extensive one-dimensional- and two-dimensional nuclear magnetic resonance (NMR) experiments. Results: A new saponin, namely malonyl-ginsenoside Re, was isolated from the fresh flower buds of Panax ginseng, along with malonyl-ginsenosides Rb1, Rb2, Rc, Rd. Some assignments for previously published $^1H$- and $^{13}C$-NMR spectra were found to be inaccurate. Conclusion: This study reports the complete NMR assignment of malonyl-ginsenoside Re, $Rb_1$, $Rb_2$, Rc, and Rd for the first time.

Keywords

References

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