• The Korean Journal of Physiology
• /
• 제4권2호
• /
• pp.61-67
• /
• 1970

In view of the importance of sulfhydryl groups in producing a chemical protection against ionizing radiation, an attempt was made to evaluate the changes of intrinsic non-protein sulfhydryl (NP-SH) and non-Protein disulfide(NP-SS) of the lung and liver tissues of rabbits following the whole body X-irradiation with 900 r either in single or fractionated $(300\;r{\times}3)$ dose. NP-SH was measured by Ellman's method, and NP-SS was measured by the electrolytic reduction method described by Dohan. Experiment was performed at 1,3,5,24 and 48 hours post·irradiation, and the results were compared with the control. The results thus obtained are summarized as follows; 1) Intrinsic levels of NP-SH and NP-SS of normal rabbits were $0.77{\pm}0.10$ and $0.61{\pm}0.07\;{\mu}mol/gm$ wet weight in liver, and $0.28{\pm}0.03$ and $0.54{\pm}0.03$ in lung tissues respectively. 2) NP-SH of liver after single X-irradiation showed no significant change in general, but at 48 hours post·irradiation, it was elevated comparing with the normal value. 3) Levels of NP-SS in liver was decreased than the normal value in the irradiated groups, and the lowest level was observed at 3 hours after single X-irradiation and at 5 hours after fractionated irradiation. 4) In lung tissues, levels of NP-SS showed no significant change from the control at earlier experimental hours, but a great decrease was observed at later Part of the experiment.

• Journal of Radiation Protection and Research
• /
• 제39권3호
• /
• pp.134-141
• /
• 2014

방사선요법은 항암 치료에서 널리 이용되는 요법으로, 항암치료에 저선량 방사선을 이용하는 것에 대한 관심이 증가되고 있고, 저선량 방사선의 다양한 생물학적 효과가 있음이 보고되고 있다. 그러므로 본 연구에서는 마우스 모델에서 저선량 방사선이 면역반응에 어떠한 영향을 미치는지, 또한 이를 감지할 수 있는지를 조사하였다. C57BL/6 마우스에 $^{137}Cs$ 선원을 이용하여 연속 3일간 총 90 mGy의 저선량 방사선을 전신 조사한 후 마지막 방사선 조사 2, 14, 28일 후에 마우스를 희생시켜 말초 혈액 세포수, 비장 세포수, 비장 내 면역세포의 비율과 활성화 정도를 분석하였다. 말초 혈액 검사를 통해, 저선량 방사선 조사군에서 적혈구와 혈소판 수의 유의적 변화는 관찰할 수 없었으며, 백혈구 수는 마지막 방사선 조사 후 2일째에 선량-의존적인 감소를 보였으나, 점차 회복되는 경향을 나타냈다. 비장세포 수도 2일째 감소를 보였지만 서서히 그 수가 증가됨을 확인하였다. 2일과 14일째에 비장세포의 Foxp3 mRNA가 감소된 반면, CD4 T 세포와 CD69 양성세포가 증가되었다. 마우스에서 분할 저선량 방사선을 전신조사한 결과, 방사선조사군에서 특이적인 임상 증상이나 유의적인 체중감소를 보이지 않았다. 본 연구에서는 마우스를 대상으로 저선량을 분할 조사하였을 경우에도 면역학적 변화를 확인할 수 있으며 이를 통해 향후 저선량 방사선의 생물학적 효과를 뒷받침하는 자료로 활용할 수 있으리라 기대한다.

• Journal of Radiation Protection and Research
• /
• 제12권2호
• /
• pp.1-8
• /
• 1987

원자력병원(原子力病院) 싸이클로트론 중성자선(中性子線)의 방사선생물학적(放射線生物學的) 특성파악을 위하여 생쥐를 이용하여 RBE를 측정하였다. 단일전신조사(單一全身照射)에 의한 생쥐공장소양선세포(空腸小襄腺細胞)의 RBE는 2.8이었다. 이 RBE치(値)는 상이(相異)한 조직(組織)이나 기관(器管)종류에 있어서 분할(分割), 조사방법(照射方法)의 변화에 따라 상이(相異)하게 변하였다. 위장관(胃腸管) 및 골수(骨髓)의 단일조사(單一照射)에 대한 RBE는 각각 1.5 및 1.9였고, 분할조사시(分割照射時) 1회, 조사량(照射量), 총조사량(總照射量) 및 분할(分割)횟수 등에 따른 RBE의 변화를 관찰하였다.

• Applied Microscopy
• /
• 제25권2호
• /
• pp.20-28
• /
• 1995

This study observes the change of small intestine mucosa paneth cell by changing the amount of radiation to rat. It uses the rat(Wistar) of 250-300g as the experimental animal and irradiation equipment is Gammacell 3000Elan System. and the irradiation is conducted for 500Rad group for 34sec., 1000rad for 68sec., and 1500Rad for 102sec. once on the whole body of each group, eachgroup is anesthetized with ether after 24hours. its small intestine is extrated and then it is observed by transmission electronic microscopy. The experimental results are as follows : 1. 500 Rad Group The Slightly elongated form of mitochondria and rough endoplasmic reticulum are observed in 500 Rad group. 2. 1000 Rad Group Golgi apparatus is appeared as the extended plasmodium, secretory granules exist only external membrane due to the self-fusion, the number of mitochondria that are changed as L-type are reduced, rough endoplasmic reticulum is distributed with the expanded form. 3. 1500 Rad Group The number of Golgi apparatus and granules is remarkably reduced, mitochondria is changed into C-type and free ribosomes can be observed instead of the reduction of rough endoplasmic reticulum.

• Advances in Traditional Medicine
• /
• 제6권3호
• /
• pp.245-251
• /
• 2006

We examined the radioprotection effects of acupoint (acupuncture point) stimulation during organogenesis stages of ICR mice. Pregnant mice received 1.5 Gy whole body X-irradiation on day 8 of gestation, which is the early stage of organogenesis. The embryonic death rate and teratogenesis rate by radiation were examined. Electroacupuncture to the leg acupoints and/ or transcutaneous stimulation to the back acupoints on the pregnant mice showed no protective effect against irradiation on embryonic or fetal death rate. On the contrary, the strong stimulation resulted in increase in the mortality after irradiation rather than protection. However acupoint stimulation to the pregnant mice never showed harmful effects by itself on embryos. It tended to reduce the skeletal malformations induced by X-ray irradiation. We suspect that acupoint stimulation removed the cells injured by irradiation during embryonic development, resulting in an increase in embryonic death rate and reduction in skeletal anomalies.

• 대한방사선기술학회지:방사선기술과학
• /
• 제33권4호
• /
• pp.321-326
• /
• 2010

본 연구에서는 미역이나 다시마에 많이 분포하면서 항산화작용이 탁월한 알긴산올리고당의 방사선 방어효과를 알아보기 위해 3 Gy 방사선이 전신 1회 조사된 마우스를 가지고 IL-6을 측정하였다. 측정 결과 방사선조사대조군과 비교하여 볼 때 소장과 간 조직 모두 방사선조사 전 7일간 알긴산올리고당의 처치를 시행한 그룹에서 IL-6 생성이 억제됨을 관찰하였다(p < 0.001). 이는 알긴산올리고당이 항산화작용을 통해 방사선이 피폭된 생체조직을 방어함으로써 IL-6의 생성을 억제한 것으로 사료된다. 결론적으로, 본 연구를 통해 알긴산올리고당의 일부 방사선 방어효과를 규명했고 아울러 화학적 독성이 없는 자연산생물이 방사선 방어제로 활용될 수 있을 가능성을 확인하였다.

• 대한핵의학회:학술대회논문집
• /
• 대한핵의학회 1999년도 제38차 춘계학술대회
• /
• pp.222-227
• /
• 1999

Re-188 is suitable for endovascular liquid-balloon brachytherapy for the prevention of restenosis after angioplasty. Re-188 was concentrated to 3700 MBq/ml and labeled with DTPA. According to dosimetric calculation, it took 420 seconds using Re-188 solution with concentration of 3700 MBq/ml to irradiate 17.6 Gy to the target at 1 mm from the balloon surface. Software was made to estimate the irradiation time. MIRD calculation with dynamic bladder model yielded the whole body dose of Re-188-DTPA as 0.005 mGy/MBq in case of balloon rupture and release of the whole amount into the blood.

• Journal of Yeungnam Medical Science
• /
• 제4권1호
• /
• pp.25-32
• /
• 1987

전복부조사에 대한 X-선의 영향을 체중과 말초혈액 변화를 분석하여 적정 분할회수 및 치료기간설정에 응용하기 위하여 도합 160마리의 백색마우스를 두개의 조사군으로 나누어 제1군은 100cGy과, 제2군은 200cGy으로 나누고 일령이 $30{\pm}3$일로서 체중은 수컷이 $25{\pm}2gm$, 암컷은 $23{\pm}1gm$의 동수를 재료로 하여 방사선조사를 시행한 후, 체중의 변화와 말초혈액 변화를 분석하여 다음과 같은 결과를 얻었다. 체중과 혈색소의 변화는 성별이나 분할조사량에 따른 현저한 차이를 발견할 수 없었다. 백혈구의 감소는 3,000cGy에서 최소치를 나타내었으며 암, 수 모두에서 100cGy군에 비해 200cGy군에서 현저하였다. 감별백혈구의 변화는 조사선량이 증가함에 따라 암, 수 모두에서 임파구의 감소가 급격하였으나 호중구는 이와 반대로 증가함을 보였으며 이런 변화는 일회 조사량이 더 많은 제1군에서 현저하였다. 위의 결과를 종합하여 보면 방사선조사 자체로 인한 체중과 혈색소의 변화는 일회조사량이나 총 조사기간에 따른 차이를 발견할 수 없었으며 일회조사량이 많은 경우에 백혈구의 감소가 더 현저하였으며 이는 주로 임파구의 감소에 기인하며 이와는 반대로 호중구의 비율증가가 나타났다. 실험적으로 분할조사량의 선정에 말초혈액상에 미치는 영향이 큼을 보여주어 실제 치료에서 분할조사량의 올바른 책정이 중요함을 시사하였다.

• 한국동물학회지
• /
• 제7권2호
• /
• pp.6-12
• /
• 1964

The present paper deals with the changes in serum protein fraction, total serum protein, hematocrit, red blood cell, haemoglobin, and weight of liver or kidney : body weight ratio of irradiated and non-irradiated snake head, Ophicephalus argus(CANTOR) . Irrardiation doses are 2537$\AA$-7 minutes (7M) and 2537$\AA$-15 minutes (15M). Serum electrophoretic patterns showed a marked decrease in albumin fraction at 1, 3, 9, 12 and 15 days on 7M group and 15 M group. On both experimental groups percentage increases in $\alpha$1 and $\beta$ fractions occurred at different time periods in general but are interpreted as only apparent changes accompanying the greater albumin fall. ${\gamma}$-Globulin decreased at 1, 6 and 15 days on 7M group. and at 1, 3, 6 and 9 days on 15M group. Also, A/G ratio was significantly low in groups subjected to above conditions as compared to the controls. A/G ratio decreased at 3 and 15 days remarkably on both groups. On the average, the reductions in the A/G ratio were not proportional to themagnitude of ultraviolet-light. Total protein of serum changed according to suggestive changes in electrophoretic patterns of serum . Total protein of serum declined at 1, 3, 6, 9 and 12 day periods on 7M groups and at 1, 3, 9, 12 and 15 day-periods on 15 M group, and increased approaching control values at 15 and 18 day-periods on both groups. Hematocrit increased remarkably at 1, 3, 6 and 12 days and decreased at 9 and 15 days on 7M group, and increased throughout the sampling period on 15M . Red blood cell decreased throughout the sampling period and increased slightly at 6 days on 7 M group and decreased at 1, 6, 9, 15 and 18 days and increased remarkably at 3 and 12 days on 15M group. Hemoglobin decreased remarkably at 1 day-period and increased at other days on both groups. The liver weight was not remarkably changed after whole body irradiation on both groups. and kidney was increased from 1st day on 7M group and 3 rd day on 15M group respectively. It appears that changes in electropphoretic patterns of serum, A/G ratio, total protein of serum, hematocrit, red blood cell, hemoglobin, and liver or kidney weight act to the detriment of the animal following non-ionizing irradiation.

• 대한방사선기술학회지:방사선기술과학
• /
• 제43권4호
• /
• pp.235-242
• /
• 2020

Scanogram is that combine several practical images into one image to observation. So it is an important consideration in many clinical situation such as iliac measurement, leg alignment measurement and Scoliosis. Currently, scanogram examinations are mainly conducted for children and elderly patients. In this study, in order to apply the longbone detector to children or elderly patients who are difficult to cooperate with, we compared the longbone detector from D equipment with the G equipment discovery 656 Puls equipment in reproducibility of images, distribution of irradiation dose, scattering dose, irradiation time and image acquisition time. D equipment took more than twice as much time as G equipment. The scattered dose generated about 50% more G equipment than D equipment. In the whole spine scanogram and the measurement length of the lower leg, D equipment was also measured longer than G equipment. However, both methods did not show much difference from the CT scanogram, so there was no problem in measurement. The height of the thyroid radiation dose of G equipment was produced more radiation than D equipment. However, the longbone detector deviated from the x-ray center line relative to the tube rotation method, and was measured lower by the directionality of the measuring instrument, so that the error could not be corrected. In the conclusion of study, using the longbone detector is excellent for applying to children or elderly patients to reduce scattering dose. However, using CR may be useful to normal patients. Because, the image quality may deteriorate due to an imbalance of dose difference in thickness depending on the body part. So, it is useful to using a compensation filter or tube rotation method when we take a whole spine scanogram.