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Study on congestion of the gills in cultured eels Anguilla japonica III. Effect of NaCl treatment on congestion of the gills in cultured eels, Anguilla japonica (뱀장어 아가미 울혈증에 관한 연구 III. NaCl처리가 울혈증 치료에 미치는 영향)

  • Choi, Min-Soon;Park, Kwan-Ha;Choi, Sang-Hoon;Kim, Young-Gill;Kim, Jong-Myeon;Cho, Jeong-Gon;Jang, Seon-Il
    • Journal of fish pathology
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    • v.8 no.2
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    • pp.111-118
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    • 1995
  • This experiment was carried out in order to elucidate the effect of NaCl(0.85%) acclimation on congestion symptoms in central venous sinuses(CVS) of gill filaments and on the alteration of blood constituents in cultured eels for 1, 2, 3, 4, 5, 6 and 7days after handling stress. After giving a handling stress on eels, the frequency of gill congestion was about 90%. It gradually decreased to 40% on the 3rd day, then increased to 60% on the 7th day in NaCl(0.85%) acclimation. The values of haematocrit(Ht), methemoglobin(Met-Hb), glucose (GLU), calcium($Ca^{++}$), lactic dehydrogenase(LDH), glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase(GOT) were lower than those of the control, whereas magnesium($Mg^{++}$) and haemoglobin(Hb) were higher than the control. However total protein(Tp) and albumin (ALB) levels similar to the control. When the fish after giving a handling stress were cultured in NaCl-free water as a control, the frequency of gill congestion and the value of blood components showed a similar pattern to that obtained from the NaCl acclimated experiments. However, in the early period(1-3day) after handling stress, NaCl acclimating slightly helped the natural tendancy of recovery. These results suggest that the frequency of gill congestion is not always reduced over the whole period of NaCl acclimation. However, the acclimation to NaCl solution may be a somewhat useful therapy for the gill congestion in stressed eels.

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Effect of Enteromorpha compressa on the physiological activities in carp, Cyprinus carpio (파래투여에 의한 잉어류의 생리활성에 관한 연구)

  • Choi, Min-Soon;Park, Kwan-Ha;Choi, Sang-Hoon;Kim, Jong-Yeon;Kim, Jong-Myeon;Cho, Jeong-Gon;Jang, Seon-Il
    • Journal of fish pathology
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    • v.8 no.2
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    • pp.149-156
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    • 1995
  • Physiological activities of E. compressa were examined after oral and intraperitoneal(i.p.) adminstration in young(8g) Israeli and colored carp. Hematological parameters were evaluated to test physiological respose. Anti-bacterial activity was examined by counting the number of bacterial cells in the kidney, and also by measuring the change of agglutinin titers following A. sobriae infection. There was a tendency of increase in E. compressa-fed groups in total protein, albumin and glucose levels. The most marked increase was noted in the group fed with 5% E. compressa. GPT and GOT levels were reduced with the increase of E. compressa concentration. Feeding of E. compressa did not alter haematocrit(Ht) and hemoglobin (Hb) values. The number of A. sobriae was reduced in all groups intraperitoneally treated with variable concentration of E. compressa-extract. The lowest bacterial cells were found at the group intraperitoneally treated with $30{\mu}g$ of E. compressa-extract per g of colored carp($30{\mu}g/g$), indicating that the anti-bacterial activity is maximized at this concentration. The agglutinin titers were elevated in E. compressa extract-treated groups($30{\mu}g/g$) with the maximum value of $6.0{\pm}1.1$. These results indicate that E. compressa adminstration activated physiological response, and triggered a cascade for anti-bacterial reaction.

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[ $2{\beta}$ ], $3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic Acid Induces the Apoptosis of Human Hepatoma HepG2 Cells ($2{\beta}$, $3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid처리에 의한 인간 간암세포주 HepG2의 apoptosis 유도)

  • Yoo, Ki-Hyun;Lee, Jong-Min;HwangBo, Jeon;Song, Myoung-Chong;Yang, Hye-Joung;Baek, Nam-In;Kim, Soung-Hoon;Kim, Dae-Keun;Kwon, Byoung-Mok;Park, Mi-Hyun;Chung, In-Sik
    • Applied Biological Chemistry
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    • v.49 no.4
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    • pp.270-275
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    • 2006
  • [ $2{\beta},\;3{\alpha}$ ], 23-trihydroxyrus-12-ene-28-oic acid was isolated from Trapa pseudoincisa S. et Z. It has a common structure of pentacyclic triterpenes and belongs to the amyrin ursolic acid group. The cytotoxic effect of this compound was investigated in human hepatoma cell line HepG2. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid showed dose-dependent cytotoxicity in HepG2 cells. Confocal microscopy data showed that green fluorescence was increased in $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid treated-HepG2 cells in a time-dependent manner. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid also increased the sub-G1 cell population of HepG2 cells as well as ladder-like DNA fragmentation. Taken together, our results indicate that $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid induced apoptosis in HepG2 cells.

The Demographic Position of the Koreans in China (재중 교포의 인구학적 위치)

  • Park, Chai-Bin
    • Journal of Preventive Medicine and Public Health
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    • v.22 no.1 s.25
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    • pp.14-24
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    • 1989
  • 현재 중국에는 55개의 소수민족이 있으며, 그 인구총수는 6,700만을 초과한다. 중국정부는 특히 70년대 후반서부터 소수민족에 대한 일연의 보호정책을 쓰고 있으며 다수민족인 한족에 대하여 엄격히 실시하는 1가구 1자녀 정책에서도 소수민족을 제외하고 있다. 이에 따라 소수민족의 증가는 현저하게 늘었다. 그러나 전반적인 인구학적특성으로 보면 소수민족은 아직 후진성을 면하지 못하고 있다. 조선족이라고 일컫는 재중 우리교포는 한족을 포함하여 중국내 어느 민족과 비교하여도 인구학적으로 독특한 위치를 차지하는 경우가 많다. 예를 들어 인구증가율은 가장 낮으며 어느민족보다 앞서서 1970년대 중반에 이미 대치수준 이하의 출생률에 도달한 것으로 믿어지고 있다(Han 등, 1988). 문맹률은 최하이며 대학수업율은 최고로 높다(장(張), 1984). 사망률은 전소수민족중 가장 낮고 평균 수명은 가장 길다(Dowdle, 1984). 이렇게 재중 조선족은 인구학적으로 가장 선진적이기 때문에 1자녀 정책에 대한 수용 준비가 다른 민족보다 앞서 있다고 할 수 있겠다. 물론 조선족은 소수민족에 속하기 때문에 1자녀정책에 구애될 필요가 전혀 없다. 따라서 조선족 가정에서 1자녀만을 낳겠다는 서약을 하여 이른바 '독생자녀증'을 영수하면 이것은 한족의 경우와 달리 자율의사에 따른 것이라 할 수 있을 것이다. 이 논문에서는 다음 두가지를 다루기로 하겠다. 즉, 조선족이 인구학적으로 보아 딴 민족에 비하여 어떤 위치에 있으며, 또 중국의 중요인구정책인 1가정 1자녀제에 어떻게 반응하고 독생자녀증을 어떻게 수용하느냐 하는 것이다. 이 밖에 중국의 대소수민족 인구정책의 앞날에 대하여도 약간의 전망을 하여볼까 한다.스와 여기에 사용되는 신호 방식을 논의하고 있다. 그 외에도 전광 통신망 운용에 필요한 라우팅 및 파장할당 방법과 OXC를 이용한 그물형 망에서의 보호 및 복구에 대한 연구도 활발히 진행되고 있다.rotene 보충군에 비하여 유의적으로 높았으며 retinyl acetat와 B-carotene 보충군 사이에는 유의적인 차이가 없었다.3.17%로 가장 양호하였으나, 양파망과 염화비닐판을 수직으로 놓은 것은 각각 1.52%와 1.61%로 비교적 저조하였다. 수정 후 40일째부터 90일째까지 측정한 부착치패의 경과 일수에 따른 각장의 성장은 $SL=184.44e^{0.0335X}(r^2=0.9861)$의 회귀직선식으로서 나타났다. 중간육성 시험에서 수심별 성장을 분석한 결과, 비단가리비 치패는 저층보다 표층이 각장 5.92mm, 전중량 6.07g 정도 더 빨리 성장하였다. 시간으로 하여 l 주 44 시간을 기준으로 측정하면 통상질환관리 18.56시간, 지역사회 보건관리 5.67 시간, 모자보건 및 가족계획 5.52 시간, 사업 운영관리 및 지도 4.10시간, 지역사회 조직 및 개발 3.05 시간, 보건정보체계 개발 및 수집 2.94 시간, 사업계획 수립 2.89시간의 순으로 나타났다. 5) 보건진료원의 업무영역별 수행 소요시간의 상판판계를 살펴보면 지역사펴 조직 및 개발을 위 해 소요한 시간은 사엽계획 수립 소요시간 및 보건정 보체계 관리 소요시간과 순상관관계를, 사업 계획 수립 소요시간은 지역사회 보건관리, 모자보건 및 가족계획 관리 소요시간 및 보건정보체제 관리 소요시간과 순상관관계를 나타냈다. 또한 통상질환관리 소요시간은 지역 사회 조직 및 개발, 사업계획 수립, 지역사회 보건관리와 모자보건

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Mesh selectivity of monofilament and multifilament nylon gill net for Marbled sole (Pleuronectes yokohamae) in the western sea of Korea (서해안 문치가자미 자망의 망지재료에 따른 망목선택성)

  • Kim, In-Ok;Park, Chang-Doo;Cho, Sam-Kwang;Kim, Hyun-Young;Cha, Bong-Jin
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.46 no.4
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    • pp.281-291
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    • 2010
  • To estimate the mesh selectivity of monofilament and multifilament gill net for Marbled sole, Pleuronectes yokohamae, the field testes were carried out 12 times with five different mesh sizes (86.6, 101.0, 121.2, 137.7, 151.5mm) in the western sea of Korea, 2007-2009. The master curve of selectivity was estimated by the extended Kitahara's method. In the field testes, the total number of species and catch were 26 and 987, respectively. The catch number of marbled sole was 728 and occupied 73.8% in total catch. The optimum values of l/m for 1.0 of retention probability in monofilament and multifilament gill net were estimated 0.288 and 0.307, respectively and l/m was estimated to be 0.189, 0.203, 0.213, 0.222 and 0.230 in case of monofilament gill net and 0.171, 0.191, 0.205, 0.216 and 0.227 in case of multifilament gill net when the retention probability were 0.1, 0.2, 0.3, 0.4 and 0.5, respectively. The 0.5 selection range of multifilament gill net was wider about 1.5 times than that of monofilament gill net according as multifilament gill net was 0.216 and monofilament gill net was 0.148. So the multifilament gill net has a low selectivity than that of the monofilament gill net. To estimate the optimum mesh size on first maturity length 19.5cm of marbled sole, the retention probability value of 0.1 was adopted in consideration of using the gill net for marbled sole at present. The optimum mesh size were estimated to be 103.2mm and 114.0mm in monofilament gill net and multifilament gill net, respectively, on first maturity length 19.5cm of marbled sole.

A Study on Estimation of Design Tidal level Considering Sea Level Change in the Korean Peninsula (한반도의 해수면 상승을 고려한 설계조위 산정에 관한 연구)

  • Choo, Tai Ho;Sim, Su Yong;Yang, Da Un;Park, Sang Jin;Kwak, Kil Sin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.4
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    • pp.464-473
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    • 2016
  • The air temperatures of the coast and inland are rising due to an increase in carbon dioxide emissions and abnormal climate phenomena caused by global warming, El Nino, La Nina and so on. The sea levels of the Earth are rising by approximately 2.0 mm per year (global average value) due to the thermal expansion of sea water, melting of glaciers and other causes by global warming. On the other hand, when it comes to designing a hydraulic structure or coastal hydraulic structure, the standard of the design water level is decided by analyzing four largeness tide values and a harmonic constant with the observed tidal water level or simulating numerical model. Therefore, the design tidal water level needs to consider an increasing speed of the seawater level, which corresponds to the design frequency. In the present study, the observed tidal water levels targeting 46 tidal stations operated by the Korea Hydrographic and Oceanographic Administration (KHOA) from the beginning of observations to 2015 per hour were collected. The variation of the monthly and yearly and increasing ratio were performed and divided into 7 seas, such as east and west part of the Southern Sea, south part and middle of the East Sea, south part and middle of the Western Sea, and Jeju Sea. The current study could be used to determine the cause of local seawater rises and reflect the design tidal water level as basic data.

A Preliminary Study on the Exhumation Mechanism of the Paleozoic Gwangcheon Gneiss in the Southwestern Margin of the Gyeonggi Massif (경기육괴 남서 연변부에 발달하는 고생대 광천편마암의 노출기작에 대한 예비 연구)

  • Park, Seung-Ik
    • Economic and Environmental Geology
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    • v.50 no.6
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    • pp.525-535
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    • 2017
  • Exhumation mechanism of migmatite in orogenic belts provides insights into thermo-mechanical evolution of lithosphere in association with orogeny. This study deals with kinematics of structures in and around the Gwangcheon Gneiss, as a preliminary study on exhumation mechanism, which is a main constituent of a domal structure (viz., Oseosan Dome) in the Hongseong area, southwestern margin of the Gyeonggi massif. Geological structures in the Gwangcheon Gneiss, which mainly comprises southern and northwestern part of the Oseosan Dome, generally have kinematic component of top-outward shear. This feature is likely to represent diapiric dome-up movement. In addition, a high strain zone, by which the tectonic domain involving the Gwangcheon Gneiss is bounded on the west, show structural features with normal sense of shear component. Taking available (thermo)chronological data into account, it is interpreted that activation of the high strain zone and exhumation of the Gwangcheon Gneiss occurred during Late Triassic, when the Gyeonggi massif was widely affected by post-collisional processes. It means that the Gwangcheon Gneiss was diapirically moved up and exhumed in the footwall of extensional high strain zone in association with Triassic post-collisional processes.

Effects of micro-topography on the crown growth of Picea jezoensis under different wind conditions on Mt. Deokyu, Korea (미지형과 바람이 덕유산 가문비나무(Picea jezoensis)의 수관생장에 미치는 영향)

  • Han, Ah Reum;Jung, Jong Bin;Park, Pil Sun
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.4
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    • pp.277-285
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    • 2019
  • This study was conducted to understand the growth characteristics of Picea jezoensis that was one of representative subalpine species in Korea, in the light of the effects of wind and micro-topography in its habitats on Mt. Deogyu, Korea. The prevailing wind directions were southwest and west at the study sites. We randomly selected P. jezoensis (height ≥ 1.5 m) on windward, intermediate, and leeward slopes and measured diameter at breast height (DBH), height, crown length by 8 directions in upper, middle and lower sectors of the crown. We examined the micro-topography of P. jezoensis stood on and classified it into 3 types: type 1 was mounded and fully exposed to surrounding environments without neighboring trees; type 2 was somewhat wind-protected by surrounding rocks, but no neighboring vegetation; type 3 was on gentle slope or flat where P. jezoensis grew with neighboring trees or shrubs. The ratios of height to DBH, and estimated crown growth to actual crown growth (hereafter crown growth ratio) were compared among the three types of micro-topography on windward, intermediate and leeward sites. The height growth per DBH and crown growth ratio in the upper and middle crowns were the smallest on the windward site, and the highest on the leeward site (p < 0.01). The crown growth ratio on type 1 on the windward site was only 46% of that on type 3 on the leeward site. Although on the same windward slope, trees on type 1 had more deformed crown shapes than that on Type 3, showing asymmetric crown cross-sectional areas. Wind and micro-topography played critical roles on the crown growth of P. jezoensis.

Anti-oxidative and Anti-cancer Activities of Treculia africana Extract in Human Colon Adenocarcinoma HT29 Cells (대장암세포주 HT29에서의 Treculia africana 추출물의 항산화 및 항암 활성 분석)

  • Oh, You Na;Jin, Soojung;Park, Hyun-jin;Kim, Byung Woo;Kwon, Hyun Ju
    • Journal of Life Science
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    • v.25 no.5
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    • pp.515-522
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    • 2015
  • Treculia africana Decne, a breadfruit species, is native to many parts of West and Tropical Africa. The breadfruit belongs to the family Moraceae and is one of the four members of the genera Treculia. The crude extract of T. africana has been used in folk medicine as an anti-inflammatory agent for various ailments, such as whooping cough. In this study, we evaluated the anti-oxidative and anti-cancer activities of the methanol extract of T. africana Decne (META) and the molecular mechanisms of its anti-cancer effects in human colon carcinoma HT29 cells. The META exhibited anti-oxidative activity through a DPPH radical scavenging capacity and inhibited cell growth in a dose-dependent manner in HT29 cells. META treatment induced apoptosis of HT29 cells, showing an increase in the percentage of both SubG1 cells and Annexin V-positive cells and the formation of apoptotic bodies in a dose-dependent manner. META-mediated apoptosis was associated with the up-regulation of the death receptor FAS and Bax and a decrease in the Bcl-2 expression. META-treated HT29 cells also showed the release of cytochrome c from the mitochondria into the cytosol, activation of caspase-3, caspase-8, and caspase-9, and proteolytic cleavage of poly ADP-ribose polymerase (PARP). These findings suggest META may exert an anti-cancer effect in HT29 cells by inducing apoptosis through both intrinsic and extrinsic pathways.

Distribution and Species Composition of Phytoplankton in the Southern Waters of Korea and their Relation to the Character of Water Masses (한국남해의 식물플랑크톤의 분포와 수괴특성)

  • PARK Joo-Suck;LEE Sam-Geun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.3
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    • pp.208-214
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    • 1990
  • Phytoplankton samples for this study were collected by Van Dorn Sampler at 28 oceanographic stations in the southern waters of Korea in February, April and August, 1988 to characterize the distribution and species composition of phytoplankton and their relation to the movement of water masses. During the study periods, phytoplankton standing crops in the southern waters were maximum in April and minimum in February. When the geographical distribution and abundance of the phytoplankton were considered, the highest standing crops were found near the front zone formed between Cheju Island and Tsushima Island. Therefore, their abundance depends ell the location of the front zone. On the other hand, there were relatively low standing crops in the open sea off the coastal area from the front zone in February and August. The cause of the unusal low standing crops found in August at the station 205/01, the central part of the coastal area of the southern waters where the largest standing crops occurred in February and April, seems to be due to the intrusion of Tsushima warm waters. This is because the waters at the station have high temperature and salinity, poor dissolved oxygen and nutrient salts, which are identical to the characteristics of proper Tsushima warm waters. It seems that these warm waters may affect the poor productivity in this area. Seventy-two species in thirty-three genera of the phytoplankton were indentified from the samples. Among them, 61 species in 27 genera belong to diatoms and 12 species in 7 genera to dinoflagellates. The largest number of phytoplankton species occurred in August while the largest number of the tells in April. Predominant species were Eucampia zodiacus in February, Skeletonema costatum and Chaetoceros curvisetus in April and Chaetoceros affinis in August. Eucampia zodiacus which was dominant in February and April seems to be a indicator of the southern coastal waters of Korea and mixed waters west of Cheju Island.

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