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[ $2{\beta}$ ], $3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic Acid Induces the Apoptosis of Human Hepatoma HepG2 Cells  

Yoo, Ki-Hyun (Graduate School of Biotechnology, Kyung Hee University)
Lee, Jong-Min (Graduate School of Biotechnology, Kyung Hee University)
HwangBo, Jeon (Graduate School of Biotechnology, Kyung Hee University)
Song, Myoung-Chong (Graduate School of Biotechnology, Kyung Hee University)
Yang, Hye-Joung (Graduate School of Biotechnology, Kyung Hee University)
Baek, Nam-In (Graduate School of Biotechnology, Kyung Hee University)
Kim, Soung-Hoon (Graduate School of East-West Medical Science, Kyung Hee University)
Kim, Dae-Keun (Department of Pharmacy, Woo Suk University)
Kwon, Byoung-Mok (Korea Research Institute of Bioscience nnd Biotechnology)
Park, Mi-Hyun (Erom Life Co. Ltd.)
Chung, In-Sik (Graduate School of Biotechnology, Kyung Hee University)
Publication Information
Applied Biological Chemistry / v.49, no.4, 2006 , pp. 270-275 More about this Journal
Abstract
[ $2{\beta},\;3{\alpha}$ ], 23-trihydroxyrus-12-ene-28-oic acid was isolated from Trapa pseudoincisa S. et Z. It has a common structure of pentacyclic triterpenes and belongs to the amyrin ursolic acid group. The cytotoxic effect of this compound was investigated in human hepatoma cell line HepG2. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid showed dose-dependent cytotoxicity in HepG2 cells. Confocal microscopy data showed that green fluorescence was increased in $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid treated-HepG2 cells in a time-dependent manner. $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid also increased the sub-G1 cell population of HepG2 cells as well as ladder-like DNA fragmentation. Taken together, our results indicate that $2{\beta},\;3{\alpha}$, 23-trihydroxyrus-12-ene-28-oic acid induced apoptosis in HepG2 cells.
Keywords
$2{\beta}$; $3{\alpha}$; 23-trihydroxyrus-12-ene-28-oic acid; HepG2; apoptosis; cytotoxicity;
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