• Title/Summary/Keyword: Water-extract

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Antioxidant and Acetylcholinesterase Inhibitory Effect of Aged Raw Garlic Extracts (생마늘 추출 숙성물의 항산화 및 아세틸콜린에스터라아제 저해 효과)

  • Jeong, Hee-Rok;Jeong, Ji-Hee;Jo, Yu-Na;Shin, Jung-Hye;Kang, Min-Jung;Sung, Nak-Ju;Heo, Ho-Jin
    • Journal of agriculture & life science
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    • v.45 no.4
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    • pp.113-120
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    • 2011
  • In vitro antioxidant activities and acetylcholinesterase (AChE) inhibitory effects of solvent fractions from aged raw garlic extracts were investigated. Total phenolics fractioned by hexane, chloroform, ethyl acetate and water from Aged raw garlic extracts were 3.70, 23.63, 31.27 and 2.35 mg/g, respectively. We found that ethyl acetate fractions had the highest in ABTS radical scavenging activities, ferric reducing antioxidant power and inhibitory effect on auto-oxidation of linoleic acid. Intracellular ROS accumulation resulting from $H_2O_2$ treatment of PC12 cells was significantly reduced when ethyl acetate fractions were present in the medium compared to PC12 cells treated with $H_2O_2$ only. In addition, we found that ethyl acetate fractions from aged raw garlic extracts resulted in a dose-dependent manner on AChE inhibition. Consequently, our results suggest that ethyl acetate fractions from aged raw garlic extracts may be useful as decreasing agents of oxidative stress and AChE inhibitors.

Study on Antifungal Activity of Aloe arborescens M. for a Potential Bio-Pestcide (알로에(Aloe arborescens M.)의 항진균성 검정을 통한 생물농약 가능성 탐색)

  • Ko, Seong-Wook;So, In-Sup;Huh, Moo-Ryong
    • Journal of agriculture & life science
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    • v.43 no.3
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    • pp.35-44
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    • 2009
  • Antifungal activity reaction to Aloe peel and juice extracted by several solvents and concentrations against three plant pathogenic fungi as collectrichum gloeosporioides, Fusarium oxysporum, and F. solani were investigated. The Antifungal activity of Aloe extracts varied with the kinds of solvents. In case of C. gloesporioides and F. solani, it's the highest in water, second in methanol, and third in ethanol. It showed that Antifungal activity of Aloe peel and juice extracts based on concentration was much more excellent in juice extracts than peel extracts against C. gloeosporioides, F. solani. Effect of Antifungal against F. oxysporum by juice extracts did not appear at a concentration of $100{\mu}l/L$ and then shows 9.5% of inhibition rate at $100,000{\mu}l/L$ while against C. gloeosporioides it starts to react at $100{\mu}l/L$ and then reaches at 29.3% of the highest inhibition rate at a concentration of $100,000{\mu}l/L$ out of three plant pathogenic fungi. The effect of control efficacy against Fusarium wilt caused by F. oxysporum by juice extracts of foliar spray tends to increase and the more the concentration of juice extracts increases, the higher the effect of control efficacy against Fusarium wilt disease. Foliar spray at a concentration of 10g/L has a 62% of control efficacy compared with control treatment, which foliar spray of Aloe juice extracts can be the more effective method in order to prevent Fusarium wilt disease caused by F. oxysporum.

Quality Characteristics of Yanggaeng Added with Paprika Powder (파프리카 분말을 첨가한 양갱의 품질 특성)

  • Park, Eun-Young;Kang, Seon-Gyeong;Jeong, Chang-Ho;Choi, Sang-Do;Shim, Ki-Hwan
    • Journal of agriculture & life science
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    • v.43 no.4
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    • pp.37-43
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    • 2009
  • This study investigated the quality characteristics of Yanggaeng with addition of paprika powders prepared by Special cultivar and Fiesta cultivar, respectively. The proximate composition, water activity, color, texture and sensory evaluation of Yanggaeng containing 1, 2, and 4% of each paprika powder were measured. A proximate composition of Yanggaeng with addition of paprika powders showed in the order of nitrogen free extract, moisture, crude protein, crude fat, and crude ash. The moisture of Special and Fiesta cultivar showed about 0.9. The lightness of Yanggaeng decreased with increasing addition levels of paprika powder and redness of Special cultivar increased with increasing addition levels of paprika powder. And yellowness of Yanggaeng increased with increasing addition levels of paprika powder. In the texture profile analysis, the hardness and adhesiveness of two Yanggaeng increased with increasing addition levels of paprika powder. Also the overall sensory evaluation showed best acceptability when paprika powder was added at 1% level into Yanggaeng.

Preparation and Its Taste-Active Components of Grass puffer (Takifugu niphobles) Extracts (복섬 엑스분의 추출 및 정미발현성분의 조성)

  • Yun, Jae-Ung;Hwang, Seok-Min;Oh, Dong-Hun;Nam, Gi-Ho;Choi, Jong-Duck;Oh, Kwang-Soo
    • Journal of agriculture & life science
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    • v.43 no.6
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    • pp.95-103
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    • 2009
  • To develop natural intermediate flavoring substances, optimal hydrolysis conditions and taste compounds for two step enzyme hydrolysate(TSEH) using Grass puffer(Takifugu niphobles) were investigated. The optimal conditions for TSEH method were revealed in temperature at $55^{\circ}C$ 3 hour digestion with Alcalase (pH 7.5) at the 1st step and 2 hour at $45^{\circ}C$ digestion with Flavourzyme(pH 6.0~6.5) at the 2nd step. TSEH method was superior to hot-water extraction on the aspect of yield, nitrogen contents and organoleptic taste quality such as umami and control of bitter taste formation. In taste active-components in Glass puffer TSEH, total free amino acid content was 4,502 mg%, major free amino acids were Pro, Leu, Lys, Hypro, Tau, Arg, Phe, Ala, Glu and Val in ordor. As for nucleotides, IMP(372 mg%) was the principal component and also contents of TMAO, total creatinine, and betaine were 43, 278 and 41 mg% in Glass puffer TSEH, respectively. The major inorganic ions in TSEH were Na(949 mg%), K(222 mg%), Cl(1,180 mg%) and $PO_4$(1,081 mg%).

Current Status of Hyperspectral Remote Sensing: Principle, Data Processing Techniques, and Applications (초분광 원격탐사의 특성, 처리기법 및 활용 현용)

  • Kim Sun-Hwa;Ma Jung-Rim;Kook Min-Jung;Lee Kyu-Sung
    • Korean Journal of Remote Sensing
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    • v.21 no.4
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    • pp.341-369
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    • 2005
  • Hyperspectral images have emerged as a new and promising remote sensing data that can overcome the limitations of existing optical image data. This study was designed to provide a comprehensive review on definition, data processing methods, and applications of hyperspectral data. Various types of airborne, spaceborne, and field hyperspectral image sensors were surveyed from the available literatures and internet search. To understand the current status of hyperspectral remote sensing technology and research development, we collected several hundreds research papers from international journals (IEEE Transactions on Geoscience and Remote Sensing, International Journal of Remote Sensing, Remote Sensing of Environment and AVIRIS Workshop Proceedings), and categorized them by sensor types, data processing techniques, and applications. Although several hyperspectral sensors have been developing, AVIRIS has been a primary data source that the most hyperspectral remote sensing researches were relied on. Since hyperspectral data have very large data volume with many spectral bands, several data processing techniques that are particularly oriented to hyperspectral data have been developed. Although atmospheric correction, spectral mixture analysis, and spectral feature extraction are among those processing techniques, they are still in experimental stage and need further refinement until the fully operational adaptation. Geology and mineral exploration were major application in early stage of hyperspectral sensing because of the distinct spectral features of rock and minerals that could be easily observed with hyperspectral data. The applications of hyperspectral sensing have been expanding to vegetation, water resources, and military areas where the multispectral sensing was not very effective to extract necessary information.

Quality Characteristics of Korean Red Ginsneg Powder on Pulverizing Methods (분쇄방법에 따른 고려홍삼분말의 품질특성)

  • Seo, Chang-Hoon;Lee, Jong-Won;Do, Jae-Ho;Chang, Kyu-Seob
    • Journal of Ginseng Research
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    • v.26 no.2
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    • pp.79-84
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    • 2002
  • In this study, cell cracker method as a non-collision method was evaluated for the possibility in manufacturing red ginseng powder. In color value of red ginseng powder, the L values indicating brightness were 68.18 for hammer mill (group A) and 72.08 for cell cracker (group B). The a values (redness) and b values (yellowness) were 4.21 26.56 for group A and 5.73, 28.36 for group B, respectively. As an extraction time increased, the absorbances at 420 nm of water extract of both groups were increased. In the loose bulk density, tapped bulk density and volume reduction radio indication the of powder in group A were less than those in group B. In both groups, the angles of side of red ginseng powder were shown less value than the angles of side of the powder. These two angles in group A were lets than those in group B. In sensory evaluation, the touchness between both groups was, not observed significant difference. In the contrary, the color and flavor, main factors affecting the overall quality of red ginseng powder product of group B were shown superior to those of group A. The overall preference of group B was shown higher than that of group A.

Protective Effect of Marine Natural Products against UVB-induced Damages in Human Skin Fibroblast via Antioxidant Mechanism (자외선으로 유도된 섬유아세포 손상에 대한 해양소재 추출물의 항산화 보호효과)

  • Jang, Jung-Hee;Lee, Chan;Kim, Sang-Chan;Chung, Ji-Wook;Park, Chan-Ik
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.36 no.1
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    • pp.79-87
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    • 2010
  • Ultraviolet is the one of the main environmental factors promoting aging process via increased intracellular generation of reactive oxygen species (ROS) and decreased expression of endogenous antioxidant enzymes and molecules. Therefore, in this study, we tried to search for natural skin-protective antioxidant materials from marine origins (Porphyra Thalli, Laminariae japonicae thallus, Ostreae Concha, Sargassum Thallus, Undaria thallus, Haliotidis Concha, Codium thalli, Syngnathoides biaculeatus, Hippocampus, Stichopus Stichopus, Thalli, Hizikia fusiforme thalli) which exhibit free radical scavenging activity and protect against UVB-induced cytotoxicity and oxidative cell death. Free radical scavenging activity was shown in order of Undaria thallus. Sargassum Thallus, Laminariae japonicae thallus, Hippocampus, Haliotidis Concha, Ostreae Concha, Syngnathoides biacuJeatus. In another experiment, UVB-induced cytotoxicity and cell death were effectively suppressed by treatment of Sargassum Thallus, Haliotidis Concha, Codium thalli, or Hippocampus water extract. Furthermore, UVB-induced cell death was mediated by intracellular accumulation or ROS, which was significantly inhibited by treatment with aforementioned extracts. The protective effect of these marine natural products seemed to be mediated by increased expression of antioxidant enzymes such as catalase, superoxide dismutase, and heme oxygenase-1. These results suggest that Sargassum Thallus, Haliotidis Concha, Codium thalli, and Hippocampus may have preventive and protective potentials as new functional cosmetics against oxidative stress-mediated skin damages and aging with antioxidant properties.

Effect Naetakchungumsankamibang on Skin tumor induced by 3-MCA and Immunological Response (內托千金散加味方이 3-MCA로 誘發된 皮膚癌 및 免疫調節作用에 미치는 影響)

  • Kim, Hee-taek;Roh, Seok-seon
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.12 no.2
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    • pp.20-52
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    • 1999
  • In order to investigate the effect of Naetakchungumsankamibang(NTCGS) water extract on the skin tumor induced by 3-MCA and immunological responses in mice, the cytotoxicity against SK-MEL-2 cells and total number of tumors induced by 3-MCA were measured. The numbers of WBC, platelets and RBC, plaque forming cells, hemagglutinin titer, hemolysis titer, carbon clearance, proliferation of splenocyte by thymidine uptake assay, splenic leukocyte by FACS analysis and $TNF-{\alpha}$ were also measured for the evaluation of the immunological responses. The results were obtained as follows: 1. In cytotoxicity against SK-MEL-2 cells, concentration inhibiting cell growth up to below $20\%$ of control was recognized at 1mg/ml of NTCGS. 2. In Inhibitory effect on the skin tumor induced by 3-MCA, the results showed a strong inhibitory effect of NTCGS. 3. In hematological changes in the tumor bearing mice, the numbers of WBC decreased significantly in NTCGS treated group as compared with control. 4. In hematological changes in the tumor bearing mice, the numbers of platelets increased significantly in NTCGS treated group as compared with control. 5. In hematological changes in the tumor bearing mice, the numbers of RBC increased with no significance in NTCGS treated group as compared with control. 6. Effects of the plaque forming cells in the tumor bearing mice, NTCGS treated group exhibited a significant effect compared with control. 7. In terms of the effects on hemagglutinin titer, NTCGS treated group showed higher level than control, without significance. 8. In terms of the effects on hemolysis titer, NTCGS treated group showed higher level than control, without significance. 9. In terms of the effects on phagocytic index K in Balb/C mice, NTCGS treated group showed significant difference from control. 10. In terms of the effects on proliferation of splenocyte by thymidine uptake assay, NTCGS showed significant effect at the concentration of 0.5mg/ml. 11. In terms of the effects on splenic leukocyte of Balb/C mice by FACS analysis, NTCGS treated group showed significantly higher level of helper T cell, B cell and macrophage than in control. 12. In terms of the effects on the secretion of $TNF-{\alpha}$, the treated group showed significant effect at the concentration of 1mg/ml of NTCGS. Based on the results summarized above, NTCGS is considered to have antitumor activity and immunological responses against skin tumor, and to be usable fur the treatment.

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Effects of Cattail Pollen Powders on the Rheology of Dough and Processing Adaptability of White Pan Bread (부들화분을 첨가한 밀가루 반죽의 물성과 제빵 적성)

  • Lee, Bung-Chan;Joung, Yong-Myeon;Hwang, Seong-Yun;Lee, Jong-Hwa;Oh, Man-Jin
    • Food Science and Preservation
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    • v.16 no.4
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    • pp.525-533
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    • 2009
  • To explore cattail pollen powder as a functional food ingredient, we analyzed the general components of pollen powder, tested changes in the physical properties of dough containing the powder, and investigated the process ability of powder-containing dough in bread making by adding 3%, 6%, or 9% by weight of pollen powder to wheat flour. Cattail pollen powder consisted of (all w/w) 12.7-13.2% water, 15.7-17.8% crude protein, 1.3% crude fat, 7.5-7.7% free sugar, 14.7-18.6% crude fiber, 3.4-4.9% pollen, and 49.7-55.9% soluble nitrogen-free extract (NFE). Analysis of the physical properties of dough mixed with pollen powder showed that as more pollen powder was added, the absorption rate increased, but dough stability decreased. With increasing levels of cattail pollen powder, the falling number decreased, and amylase activity increased. Fermentability was highest in dough made with 3% by weight of pollen powder, and the bread product made from such dough had the greatest volume. As more cattail pollen powder was added, the moisture activity in dough tended to decrease to a greater extent than seen in control dough, and this tendency increased with time. We found that longer storage periods were associated with greater hardness and springiness, which indicated degradation in product quality. Therefore, it is suggested that bread products containing cattail pollen powder should be consumed within 3 days of preparation. In a taste survey, bread baked with 3% (w/w) cattail pollen powder scored highest in all questionnaire items.

Antioxidative activity of roasted Pueraria lobata root extracts (로스팅 칡 추출물의 항산화 활성)

  • Choi, Goo-Hee;Kim, Hyun Jung;Park, In-Jae;Kim, Bong-Gyun;Kim, Hoi-Yeong;Jeong, Jae-Hyun;Cho, Ju-Hyun
    • Food Science and Preservation
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    • v.24 no.3
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    • pp.440-445
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    • 2017
  • We evaluated the antioxidative activity of extracts of P. lobata root depending on roasting conditions. P. lobata roots were roasted at three different temperature at $150^{\circ}C$, $200^{\circ}C$, and $250^{\circ}C$ and three different time at 10 min, 20 min, and 30 min respectively. Roasted P. lobata root was extracted using water at $85^{\circ}C$ for 6 h and filtered using filter paper, followed by then evaporated ($12{\pm}0.3^{\circ}Brix$) and freeze-dried. The concentration of maker compound puerarin was determined using a high performance liquid chromatography system. 2 phenolic compounds, flavonoid contents, and antioxidant activities of the extract powder were evaluated. Puerarin contents, Phenolic compounds, and flavonoid contents of roasted P. lobata root were higher than those of unroasted P. lobata root. The results of DPPH and ABTS showed that roasted P. lobata root possessed higher antioxidant activity than unroasted P. lobata root. This study suggested that roasting process could be applied to P. lobata root in order to achieve its high quality and functionality.