Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
권호 표지

Antioxidant and Acetylcholinesterase Inhibitory Effect of Aged Raw Garlic Extracts

생마늘 추출 숙성물의 항산화 및 아세틸콜린에스터라아제 저해 효과

  • Jeong, Hee-Rok (Dept. of Food Sci. & Techn., Gyeongsang National Univ.(Insti. of Agric. and Life Sci.)) ;
  • Jeong, Ji-Hee (Dept. of Food Sci. & Techn., Gyeongsang National Univ.(Insti. of Agric. and Life Sci.)) ;
  • Jo, Yu-Na (Dept. of Food Sci. & Techn., Gyeongsang National Univ.(Insti. of Agric. and Life Sci.)) ;
  • Shin, Jung-Hye (Namhae Garlic Research Institue) ;
  • Kang, Min-Jung (Namhae Garlic Research Institue) ;
  • Sung, Nak-Ju (Namhae Garlic Research Institue) ;
  • Heo, Ho-Jin (Dept. of Food Sci. & Techn., Gyeongsang National Univ.(Insti. of Agric. and Life Sci.))
  • 정희록 (경상대학교 식품공학과(농업생명과학연구원)) ;
  • 정지희 (경상대학교 식품공학과(농업생명과학연구원)) ;
  • 조유나 (경상대학교 식품공학과(농업생명과학연구원)) ;
  • 신정혜 ((재)남해마늘연구소) ;
  • 강민정 ((재)남해마늘연구소) ;
  • 성낙주 ((재)남해마늘연구소) ;
  • 허호진 (경상대학교 식품공학과(농업생명과학연구원))
  • Received : 2011.07.04
  • Accepted : 2011.08.25
  • Published : 2011.08.31
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Abstract

In vitro antioxidant activities and acetylcholinesterase (AChE) inhibitory effects of solvent fractions from aged raw garlic extracts were investigated. Total phenolics fractioned by hexane, chloroform, ethyl acetate and water from Aged raw garlic extracts were 3.70, 23.63, 31.27 and 2.35 mg/g, respectively. We found that ethyl acetate fractions had the highest in ABTS radical scavenging activities, ferric reducing antioxidant power and inhibitory effect on auto-oxidation of linoleic acid. Intracellular ROS accumulation resulting from $H_2O_2$ treatment of PC12 cells was significantly reduced when ethyl acetate fractions were present in the medium compared to PC12 cells treated with $H_2O_2$ only. In addition, we found that ethyl acetate fractions from aged raw garlic extracts resulted in a dose-dependent manner on AChE inhibition. Consequently, our results suggest that ethyl acetate fractions from aged raw garlic extracts may be useful as decreasing agents of oxidative stress and AChE inhibitors.

본 연구에서는 생마늘 추출 숙성물의 용매 분획물에 대한 항산화 효과와 acetylcholinesterase (AChE) 저해 효과에 대해서 조사하였다. 생마늘 추출 숙성물의 헥산, 클로포름, 에틸아세테이트 및 물 분획물을 통한 총 페놀성 화합물의 함량은 각각 3.70, 23.63, 31.27, 2.35 mg/g으로 나타났다. 또한, 에틸아세테이트 분획물이 ABTS radical scavenging activity, ferric reducing antioxidant power 및 linoleic acid를 활용한 지질의 자동산화 저해 효과에서 가장 높다는 것을 확인하였다. 추가적으로, PC12 신경 세포에서의 에틸아세테이트 분획물의 처리가 $H_2O_2$가 유발시키는 산화적 스트레스의 수준을 감소시켰다. 결국 생마늘 추출 숙성물의 에틸아세테이트 분획물이 농도 의존적으로 AChE를 저해한다는 것을 확인하였다. 그러므로 본 연구는 생마늘 추출 숙성물의 에틸아세테이트 분획물이 PC12 신경세포에 있어서 산화적 스트레스 수준을 감소시키고, 또한 AChE 저해제로서 활용될 수 있는 가능성을 확인하였다.

Keywords

Acknowledgement

Supported by : 재단법인 남해마늘연구소

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