• Korean Journal of Medicinal Crop Science
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• v.10 no.3
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• pp.181-184
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• 2002

This study was conducted to select the optimum cultivar of wasabi in water condition. In three cultivar, Daruma, Simanesarai and Daihoichigo, the Daruma was superior to the others. Though total rhizome weight was similar to Daruma and Daihoichigo, rhizome of main stem of Daruma was bigger than that of Daihoichigo, specially distribute in $80{\sim}159g$ size. The allylisothiocyanate content was higher in rhizome than that of leaf, lateral rhizome and petiole. In three cultivar, the allylisothiocyanate content of rhizome, Daihoichigo, Daruma and Simanesairai was 0.3389, 0.6332, 0.3956(mg/g), respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.196-199
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• 2005

This study was conducted to find out the effect of quality of water for wasabi growth by the relationship of quality of water and plant growth. In dissolved contents of water, water of lime area was higher than basalt area at $NO_3$, Cl, Ca, and Mg. In the change soil hardness and water temperature, basalt area was lower than lime one, during wasabi cultivation. Growth characteristics and yield were higher in basalt area than lime one. The weight of rhizome in basalt and lime was 585 kg/10a, 4183kg/10a, respectively.

• Korean Journal of Medicinal Crop Science
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• v.5 no.4
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• pp.294-301
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• 1997

Fatty acid, amino acid and organic acid contents were analyzed by gas chromatography, amino acid analyzer and high pressure liquid chromatography, respectively, in order to compare the chemical constituents of upland wasabi plant propagated by seed and auxiliary bud. Total fatty acid content and fatty acid composition of upland wasabi were not affected by the propagation methods. Generally, fatty acid content of leaf was higher than that of other parts such as enlarged stem, petiole, peduncle and root. In fatty acid composition, leaf had highest content of linolenic acid, 60-63%, in plant propagated by both seed and auxiliary bud, followed by palmitic acid, oleic acid and linoleic acid in the order. Similarly, total amino acid content was not influenced by propagation methods but plant propagated by seed had higher amount of amino acid content in enlarged stem, petiole and root than that by auxiliary bud -propagated plant. A total of 17 amino acids including 7 essential amino acids were identified in both seed and auxiliary bud propagations. Like total fatty acid content and fatty acid composition, leaf contained high amount of amino acids, especially glutamic acid, asparatic acid and leucine. Organic acid contents were similar in both propagation methods. The major organic acid in upland wasabi was acetic acid (60.0-78.2%), followed by succinic acid (9.9-29.7%) and malic acid (2.9-7.9%). Maleic acid content was least (0.5-2.6%). The result indicates that content and composition of fatty acid, amino acid, and organic acid in upland wasabi were not influenced by propagation methods.

• Korean Journal of Medicinal Crop Science
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• v.9 no.1
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• pp.76-82
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• 2001

This study was conducted to find out the optimum ranges of soil physical properties for wasabi growth by the relationship of soil physical properties and plant growth. Soil bulk density and hardness were higher in decomposition of granite and river sand than hydroball. This root distribution of surface layer was higher in decomposition of granite and river sand than hydroball. Growth characteristics and yield were higher in hydroball than decomposition of granite and river sand. In inlet site, the marketable rhizome weight in decompasition of granite, river sand, hydroball were 298kg/10a, 401kg/10a, 766kg/10a, respectively. But outlet, the weight in three soils were 251kg/10a, 256kg/10a, 633kg/10a, respectively.

• Journal of Bio-Environment Control
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• v.18 no.2
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• pp.137-141
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• 2009

This experiment was investigated to effect of divided rhizome size and medium type on survival rate and growth of wasabi for 60 days in controlled growth room. In divided rhizome size of 5mm above, survival rate was 100% and their growth (plant height of 12cm and leaf number of 3${\sim}$4 per plant) was good at 30 days after wrapped-sphagnum treatment. Plant height was 20cm above and number of leaves increased in 1-2 per plant at 60 days after treatment. Survival rate and growth didn't show any effect on plant regulator of root-tone. In inorganic media (saprolite and aerated light stone) treatment, survival rate of wasabi in divided size of 5${\sim}$10mm showed 83% or above at 30 days in deep flow culture. Growth did not show significant difference of inorganic support media treatments. Therefore, it is possible for divided rhizome size of 5mm above to do production of seedlings by acclimatize for 30 days in hydroponics under controlled growth chamber.

• Horticultural Science & Technology
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• v.29 no.4
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• pp.311-316
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• 2011

This study aimed to determine the effect of EC (electrical conductivity) levels of nutrient solution in hydroponic culture on allyl-isothiocyanate (AITC) content within plant tissues, Vitamin C content and physiological responses in wasabi plant (Wasabia japonica M. 'Darma'). The 'Darma' was grown for 5 weeks with a deep flow technique (DFT) system controlled at 5 different EC levels, including 0.5, 1, 2, 3, and $5dS{\cdot}m^{-1}$. In result, the highest total content of AITC showed at EC level 5 and $3dS{\cdot}m^{-1}$ for 1 or 5- week, respectively. The total content of AITC increased about 1.2-1.4 times when the plants were grown in the EC levels between 0.5 and $2dS{\cdot}m^{-1}$, whereas the content decreased about 6 and 56 % in the EC level 3 and $5dS{\cdot}m^{-1}$, respectively. The content of AITC was relatively higher in petiole tissue, about 53 %, taken from 1 week-grown plants when the EC was controlled between 0.5 and $2dS{\cdot}m^{-1}$. Root tissue also had relatively higher content of AITC, about 45.1 %, when the EC was controlled at 3 and $5dS{\cdot}m^{-1}$. However, a 5-fold decrease in the AITC content was found in blade tissue and a 6.8-fold decrease in root when the EC was controlled at $5dS{\cdot}m^{-1}$ for 5 weeks. There was no significant difference in the vitamin C content in 1-week grown leaf tissues under the different EC level treatments; but, the content increased about 27% in 5-week grown plants at the EC level between 0.5 and $2dS{\cdot}m^{-1}$, compared to the 1 week-grown leaf tissue. Electrolyte leakage of leaf tissue taken from 3-week grown plant was 3-fold higher at the EC level $5dS{\cdot}m^{-1}$, compared to the EC level between 0.5 and $2dS{\cdot}m^{-1}$. Chlorophyll content, photosynthesis rate and transpiration rate were decreased when the EC was controlled at higher than $2dS{\cdot}m^{-1}$. Leaf water content, specific leaf area and growth were decreased when the EC was controlled at $5dS{\cdot}m^{-1}$ for 5 weeks. All the integrated results in this study suggest that the EC level of nutrient solution should be maintained at lower than $3dS{\cdot}m^{-1}$ in order to improve nutritional value and quantity required for hydroponically grown wasabi as functional vegetable.

• Journal of Food Hygiene and Safety
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• v.23 no.3
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• pp.227-232
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• 2008

The purpose of this study was to investigate the antibacterial effect of films on shelf-life and microbiological Safety of pork meat. Effects of antimicrobial films against total aerobic bacteria, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus in pork meat were evaluated during storage of 14 days at $5^{\circ}C,\;10^{\circ}C\;and\;15^{\circ}C$. Antimicrobial films were developed with addition of a natural substance, wasabi extracts(Wasabia japonica). At $5^{\circ}C$ storage, growth of total aerobic bacteria, E. coli O157:H7, L. monocytogenes were inhibited higher than at 10 and $15^{\circ}C$. Especially, the numbers of S. Typhimurium and S. aureus were increased gradually at $5^{\circ}C$ even in the control sample, and it takes more than 14 days to increase in every sample upto 6 $log_{10}cfu/g$. The higher antimicrobial effects of the films were observed at storage of $5^{\circ}C$ than at $10^{\circ}C$ and $15^{\circ}C$. There was a limit of a single treatment of antimicrobial film to prolong shelf-life of pork meat. The synergistic effect of antimicrobial film were observed with addition of refrigeration at $5^{\circ}C$.

• Korean Journal of Medicinal Crop Science
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• v.3 no.3
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• pp.181-186
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• 1995

This study was carried out to know the effect of temperature, light intensity and $CO_2$ con­centration on photosynthesis and respiration in Wasabi (Wasabia japonica Matsum). The optimum temperature for photosynthesis in Wasabi was $17{\sim}20^{\circ}C$ and dark respiration rate was increased with the increasing of tem­perature from, $15 ^{\circ}C\;to\;30^{\circ}C$. Light compensation point was $6.0\;{\mu}E\;m^{-2}s^{-1}$ in Wasabi and $36.7\;{\mu}E\;m^{-2}s^{-1}$ in Corn, and light saturation point was $600{\mu}E\;m^{-2}s^{-1}$, similar in Wasabi and Corn. $CO_2$, compensation point was 67.3ppm in Wasabi and 11.6 ppm in Corn. Photorespiration rate in Wasabi leaf at $l000{\mu}E\;m^{-2}s^{-1}$ light intensity was 3.3 mg$CO_2$, $dm^{-2}hr^{-1}$, and then was gradually decreased as light intensity decreased. Stomatal frequency was about $76\;mm^{-2}$ on the adaxial surface and $623\;mm^{-2}$ on the abaxial surface, and the size of stomata was about 1$12{\mu}m$ on the adaxial surface and $17{\mu}m$ on the abaxial surface of the leaf.

• Korean Journal of Plant Tissue Culture
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• v.25 no.4
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• pp.257-261
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• 1998

The repeated subcultures of in vitro plant materials in wasabi became highly vitrified and the capacity for multiple shoot formation from the vitrified plant materials was very low. In order to improve the quality of in vitro propagated planting materials, the experiments were carried out using culture vessels capped with membrane filter(MF). When vitrified shoots were cultured on MS medium with 0.2mg/L BA in the vessels with MF or without MF for 60 days, the shoots in the vessels with MF did not vitrified. In contrast, the shoots grown in the vessels without MF vitrified at 65%. The stomates of vitrified leaves were circular and inflated, whereas those of normal leaves acclimatizated in the vessels with MF were ovate in shape. The hardened shoots were also cultured on MS media without sucrose containing 0.01mg/L IBA in vessels with(photoautotrophic culture) or without(control) MF. Sucrose was necessary for survival of the in vitro plantlets in the vessels without MF. After 20 days of culture, the shoots in the vessels without MF on the sucrose-free media turned yellow and died. But the shoots in the vessels with MF in the sucrose-free media produced a lot of roots. When shoots were cultured on MS medium with 2% sucrose containing 0.01mg/L IBA in the vessels with(photomixotrophic culture) or without(heterotrophic culture) MF, best growth occured in photomixotrophic culture.

• Korean Journal of Medicinal Crop Science
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• v.4 no.4
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• pp.294-300
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• 1996

We performed an experiment to confirm the possibility of wasabi culture using the wat­er of the Soyang River Dam in Chunchon and the ground water in Suwon. Water mineral content of Soyang River except for P was less than that of ground water of Suwon. Dis­solved oxgen and E C of Chunchon was proper to culture wasabi but E C and dissolved oxgen of Suwon was not suitable for that. Water temp. of Soyang river was very changable by month while that of the ground water in Suwon was kept constantly. In Soyang river of Chunchon the month that water temp. show $8{\sim}18^{\circ}C$, optimal growth temp., was May to Nov. and the month that water temp. show less than $6^{\circ}C$, growth limit temp., was $Jan.\;{\sim}\;Feb.$ of Chunchon. Rhizome weight of main stem in Chunchon and Suwon was 63g and 22g per plant and rate of maketable rhizome was each 80%, 0% by culture of 32 months to include raising see­dling period of 13 months. Dry matter partitioning ratio of petiole in Soyang river of Chunchon was the highest of all others but it was lowest of all others in ground water of Suwon. Rhizome weight of main stem in Chunchon was showed possitive correlation with plant height and fresh top weight and in Suwon it was showed possitive correlation with root weight and high possitive correlation with No. of total leaves and No. of tillers.