항균 필름과 저장 온도의 돼지고기 저장성 및 미생물학적 안전성에 대한 병행 효과

Combined Effects of Antibacterial Film and Storage Temperature on Shelf-life and Microbiological Safety of Pork Meat

  • Ha, Ji-Hyoung (Department of Food Science and Technology, Chung-Ang University) ;
  • Lee, Yu-Si (Department of Food Science and Technology, Chung-Ang University) ;
  • Heo, Sun-Kyung (Department of Food Science and Technology, Chung-Ang University) ;
  • Bae, Dong-Ho (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Park, Sang-Kyu (Biomolecular Informatic Center(BMIC), Konkuk University) ;
  • Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University)
  • 발행 : 2008.09.30

초록

본 연구는 고흡습성 필름에 고추냉이 추출물을 첨가하여 제작한 항균흡습필름의 돼지고기 중 존재하는 식품위해미생물에 대한 살균효과를 확인하고자 하였다. 총호기성균 및 식품위해미생물인 Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium(NO/NA), Staphylococcus aureus를 점 접종하여 항균흡습필름으로 포장한 후 저장온도, 기간별 및 항균 필름의 코팅 횟수가 균의 성장 및 사멸에 미치는 영향을 관찰하였다. $5^{\circ}C$에서 총균수, E. cofi O157:H7, L. monocytogenes의 성장 억제 효과는 $10^{\circ}C,\;15^{\circ}C$에서 보다 우수하였다. 특히 S. Typhimurium, S. aureus는 $5^{\circ}C$에서 저온에 민감한 반응을 보이면서 6 $log_{10}cfu/g$ 수준에 도달하는데 모두 14일 이상 소요되었다. 이는 총 균수, E. coli O157:H7, L. monocytogenes가 냉장($5^{\circ}C$) 조건에서 성장이 활발하기 때문에 항균 필름의 두드러진 효과를 확인할 수 있었던 반면, S. Typhimurium, S. aureus 는 $5^{\circ}C$에서 성장이 느려 필름에 의한 항균 효과가 비교적 약하게 나타났다. 고추냉이 추출 물질이 함유된 흡습필름은 $10^{\circ}C,\;15^{\circ}C$에서 보다 $5^{\circ}C$에서 우수한 항균효과를 보였다. 세 가지 농도별 항균효과는 $5^{\circ}C$에서 두드러진 농도별 항균효과 차이를 보였으며 코팅 횟수가 늘어날수록 항균효과가 컸다. 이는 항균필름 한 가지로만은 저장기간 향상에 한계가 있고 냉장저장이라는 hurdle을 동시에 적용하여 항미생물 Synergy 효과를 보인다고 판단된다.

The purpose of this study was to investigate the antibacterial effect of films on shelf-life and microbiological Safety of pork meat. Effects of antimicrobial films against total aerobic bacteria, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus in pork meat were evaluated during storage of 14 days at $5^{\circ}C,\;10^{\circ}C\;and\;15^{\circ}C$. Antimicrobial films were developed with addition of a natural substance, wasabi extracts(Wasabia japonica). At $5^{\circ}C$ storage, growth of total aerobic bacteria, E. coli O157:H7, L. monocytogenes were inhibited higher than at 10 and $15^{\circ}C$. Especially, the numbers of S. Typhimurium and S. aureus were increased gradually at $5^{\circ}C$ even in the control sample, and it takes more than 14 days to increase in every sample upto 6 $log_{10}cfu/g$. The higher antimicrobial effects of the films were observed at storage of $5^{\circ}C$ than at $10^{\circ}C$ and $15^{\circ}C$. There was a limit of a single treatment of antimicrobial film to prolong shelf-life of pork meat. The synergistic effect of antimicrobial film were observed with addition of refrigeration at $5^{\circ}C$.

키워드

참고문헌

  1. Tak, S.B., Kim, D.H., Yoon, S.K., and Lee, Y.C.: Effects of natural preservatives and storage temperatures on quality and shelf-life of fresh pork meat. Korean J. Food Sci. Technol., 37, 557-561 (2005)
  2. Yang, J.B., Ko, M.S., and Moon, Y.H.: Effects of vacuum packaging on lipid oxidation and meat color of chilled pork. Korean J. Food Nutr., 15, 1-6 (2002)
  3. 馬相朝, 金東勳: 인스턴트 라면의 안전성에 대한 탈산소제의 효과. 韓國食品科學會誌, 12, 229 (1980)
  4. Ishikawa, S. Nakamura, and K. Fusii, T.: The modified atmosphere storage of fish products-I. Preervative effect on salted-dried horse mackerel. Bull. Tokai Reg. Fish. Res. Lab., 59, 110 (1983)
  5. Lee, K.H. and Kwon, K.S.: New Functional Food Packaging Materials and Technology. Food Ind. Nutr., 4, 1-4 (1999)
  6. Kim, H.S., Seong, L.S., and Yu, T.S.: Antimicrobial activity and food strage of LDPE ceramic film containing antimicrobial agents. Korean J. Biotechnol. Bioeng., 15, 600-604 (2000)
  7. Hale, P.W., Miller, W. R., and Smoor, J.J.: Evaluation of a heat-shrinkable copolymer film coated with imazalil for decay control of Florida Grapefruit. Tropical Science, 26, 67-71 (1986)
  8. Weng, Y.M. and Hotchkiss, J.H.: Inhibition of surface molds on cheese by polyethylene film contaning the antimycotic imazalil. J. Food Prot., 9, 29-37 (1992)
  9. Halek, G.W. and Granf, A.: Fungal inhibition by a fungicide coupled to an inomeric film. J. Food Safety, 9, 215-222 (1989) https://doi.org/10.1111/j.1745-4565.1988.tb00522.x
  10. Weng, Y.M.: Devalopment and application of food packaging films containing antimicrobial agents. Ph. D. Dissertation, Comell University, Ithaca, New York (1992)
  11. Weng, Y.M. and Hotchkiss, J.H.: Anhydrides as antimycotic agents added to polyethylene films for food packaging. Packaging Technology and Science, 6, 123-128 (1993) https://doi.org/10.1002/pts.2770060304
  12. Koichiro, Y.: Maintenance of freshness by antimicrobial packaging material using a component of horseradish. Food and Science, 35, 102-107 (1993)
  13. Ha, J.H., Lee, Y.S., Heo, S.K., Bae, D.H., Park, S.K., and Ha, S.D.: Combined Effects of Antibacterial Film and Storage Temperature on Shelf-life and Microbiological Safety of Mackerel. J. Food Hyg. Safety. 22, 317-322 (2007)
  14. KFDA. Food code. Korea Food and Drug Administration, Seoul, Korea (2006)
  15. Brown, M.H. and Baird-parker, A.C.: The microbiological examination of meat. In Meat Microbiology, 3rd ed. (Brown M. ed.) Applied Science Publishers Ltd, London, England, pp 423-520 (1982)
  16. Egan, A.F. and Grau, F.H.: Environmental conditions and the role of Brochothrix themosphacta in the spoilage of fresh and processed meat. In Psychrotroph Microorganism in Spoilage and Pathogenicity. (Roberts, T.A., Hobbs, G., Christian, J.H.B., Skovgaad, N. eds.) Academic Press, New York, USA, pp. 211 (1981)
  17. Ono, H., Tesaki, S., Tanabe, S., and Watanabe, M.: 6-Methyl-sulphinylhexyl isothiocyanate and its homologues as foodoriginated compounds with antibacterial activity against Escherichis coli and Staphylococcus aureus. Biosci. Biotechnol. Biochem., 62, 363-365 (1998) https://doi.org/10.1271/bbb.62.363
  18. Park, Y.Y., Cho, M.S., Park, S., Lee, Y.D., Jeong, B.R., and Chung, J.B.: Sinigrin contents in different tissues of wasabi and antimicrobial activity of their water extracts. Korean J. Hort. Sci. Technol., 24, 480-487 (2006)
  19. Oscar, T.P.: Predictive models for growth of Salmonella Typhimurium DT104 from low and high initial density on ground chicken with a natural microflora. Food Microbiology, 24, 640-651 (2007) https://doi.org/10.1016/j.fm.2006.11.003