• 한국미생물·생명공학회지
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• 제41권2호
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• pp.242-248
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• 2013

Endotoxins are part of the outer membrane of the cell wall of gram-negative bacteria and are continuously released during bacterial growth. Endotoxins typically induce severe sepsis and septic shock, which cause more than 50% of mortalities. Endotoxins are easily measured in the serum by the limulus amebocyte lysate (LAL) test. However, a nonspecific result is obtained, because the high concentration of serum proteins disturbs the enzyme reaction of the LAL test. In order to solve this problem, the LAL test was performed in this study after the centrifugation of the boiled serum samples to remove the impurities. As a result, among the various conditions examined, endotoxin measurement with the LAL test was the most accurate and repeatable after centrifugation of the boiled serum at $100^{\circ}C$. Moreover, the endotoxin was accurately and repeatedly measured from the prepared sera of mice that had been administered an intraperitoneal injection of purified lipopolysaccharides (LPS) or E. coli. Therefore, the application of centrifugation to remove impurities from boiled serum gives an accurate measurement of endotoxins in the sera of normal subjects or patients, and this will lead to the improved diagnosis and prevention of diseases caused by endotoxins. In addition, the centrifugation of boiled serum samples should be considered and included in the development of endotoxin test kits.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2008년도 제30회 춘계학술대회논문집
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• pp.149-152
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• 2008

30톤급 액체로켓엔진 실물형 연소기의 형상에 따른 연소특성속도에 대한 연구를 수행하였다. 본 연구에서 연소기의 형상은 연소기 헤드와 분리가 가능한 내열재 및 채널 냉각형 연소실(${\varepsilon}$=3.2), 그리고 일체형인 팽창비가 각각 3.5와 12인 재생냉각형 연소기이다. 연소압력은 약 53${\sim}$60 bar 그리고 추진제 유량은 약 89 kg/s이고, 적용된 분사기는 리세스수가 1.0인 동축 와류형이다. 설계점 연소시험에서 팽창비가 12인 일체형 재생냉각 방식의 연소기가 가장 큰 연소특성속도를 보였는데 이는 추진제인 케로신이 분무되기 전 챔버 냉각으로 인한 온도 상승에 따른 엔탈피의 증가 및 연소압력의 증가에 기인한 것이다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2007년 가을학술발표회
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• pp.665-678
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• 2007

It was applied the grouting method to fill densely the space between the bottom of the caisson and the ground with the grout mixture mixed with anti-washout admixture after locating accurately the precast caisson on three concrete landing pads but it is far different from a costly conventional method, which place concrete to build the foundation of reinforcement concrete on the spot after excavating inside of the temporary coffering wall for the bridge foundation in the sea. To verify the grouting method in advance, the full-scale trial test was performed twice on the land. After confirming the fluidity of material for the injection and some possible problems during construction and then enhancing the original design, the main process is ongoing and it has been finished 12 spots until now. The purpose of this study is to introduces for the first time in Korea the grouting method including the automatic and the manual monitoring process applied to, based on the main process of the caisson foundation finished already in the site. In a similar construction it is sincerely expected to be referred to in the future.

• 대한기계학회논문집B
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• 제32권4호
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• pp.283-289
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• 2008

An experimental investigation was conducted to investigate the heat/mass transfer for impingement/effusion cooling system with inclined jet. Jets with inclined angle of 60 are applied to impingement/effusion cooling. At the jet Reynolds number of 10,000, the experiments were carried out for blowing ratios ranging from 0.0 to 1.5. The local heat/mass transfer coefficients on the effusion plate are measured using a naphthalene sublimation method. The result indicates that the inclined jet causes the non-uniform and low heat/mass transfer compared to the vertical jet. At stagnation region, the peak position is shifted from the geometrical center of injection hole due to Coanda effect and its level is higher than that of vertical jet due to increase in turbulence intensity by steep velocity gradient near the stagnation region. Further, the secondary peak region disappears because the interaction between adjacent wall jets weakens. When the initial crossflow occurs, the distorted heat/mass transfer pattern appears. As the blowing ratio (crossflow rate) increases, the heat/mass transfer distributions become similar to those of the vertical jet. This is because the effect of crossflow is dominant compared to that of inclined jet under high blowing ratio $(M{\geq}1.0)$. At low blowing ratio $(M{\leq}0.5)$, averaged Sh value is 10% lower than that of vertical jet, whereas its value at high blowing ratio $(M{\geq}1.0)$ is similar to that of vertical jet.

• 한국유체기계학회 논문집
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• 제8권4호
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• pp.39-47
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• 2005

The present study has been performed to investigate the influences of rectangular fins on heat transfer in an impingement/effusion cooling system with crossflow. To simulate the impingement/effusion cooling system with initial crossflow, two perforated plates are placed in parallel and staggered arrangements with a gap distance of 2 times of the hole diameter. The crossflow passes between the plates, and various rectangular fins are installed on the plates. Reynolds number based on the hole diameter is fixed to 10,000 and the flow rate of crossflow is changed from 0.5 to 1.5 times of that of the impinging jet. A naphthalene sublimation method is used to obtain the heat/mass transfer coefficients on the effusion plate. Also to analyze the flow characteristics, a numerical calculation is performed. When rectangular fins are installed, the flow and heat transfer pattern is changed greatly from the case without fins. In the injection hole region, the jet impinges on effusion plate without deflection and wall jet spreads symmetrically. In the effusion region, the crossflow accelerates due to the decrease of cross-sectional area in the channel. Local heat/mass transfer coefficients are enhanced significantly compared to the case without fins. As the blowing ratio increases, the effect of rectangular fins against the crossflow becomes more significant and then the higher average heat/mass transfer coefficients are obtained than the case without fins. However, the increase of blockage effect gives more pressure loss in the channel.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2009년도 춘계 학술발표회
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• pp.419-433
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• 2009

Pressure grouting is a common technique in geotechnical engineering to increase the stiffness and strength of the ground mass and to fill boreholes or void space in a tunnel lining and so on. Recently, the pressure grouting has been applied to a soil-nailing system which is widely used to improve slope stability. The soil-nailing design has been empirically performed in most geotechnical applications because the interaction between pressurized grouting paste and the adjacent ground mass is complicated and difficult to analyze. The purpose of this study is to analyze the increase of pullout resistance induced by pressurized grouting with the aid of performing laboratory model tests and field tests. In this paper, two main causes of pullout resistance increases induced by pressurized grouting were verified: the increase of residual stress; and the increase of coefficient of pullout friction. From the laboratory tests, it was found that residual stress in borehole increases by pressurized grouting and dilatancy angle could be estimated by cavity expansion theory using the measured wall displacements. From the field test results, the pullout resistance of soil-nailing with pressurized grouting was found to be 10% larger than that of soil-nailing with gravitational grouting, mainly caused by mean normal stress increase and dilatancy effect. So, the pullout resistance could be estimated by considering these two effects. The radial displacement increases with dilatancy angle increase and the dilatancy angle decreases with injection pressure increase. The measured pullout resistance obtained from field tests is in good agreement with the estimated one from the cavity expansion theory.

• Journal of Periodontal and Implant Science
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• 제27권1호
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• pp.45-59
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• 1997

Platelet-derived growth factor(PDGF) has been shown to play an important role in periodontal regeneration. The purpose of the present study was to examine the distribution of PDGF in experimentally created periodontal intrabony defects after flap surgery with various bone graft materials. Six healthy mongrel dogs were used in this study. Three-wall bony defects were created in maxillary and mandibular premolars, inflammation induced by wire ligation and injection of impression material into the defects. Eight weeks later, the experimental lesions thus obtained were treated by plain flap surgery(control group), flap surgery plus autogenous bone graft(autogenous bone group), flap surgery plus Biocoral graft(Biocoral group), or flap surgery plus bioglass graft(bioglass group), which were randomly assigned to the defects. After 4, H, and 12 weeks postoperatively, 2 dogs were sacrificed at each time and 1he specimens were taken for histological examinations and immunohistochemical examinations for PDGF. In the control defects the amount of new bone formation was minimal. In the autogenous bone and Biocoral group new bone was deposited around implanted particles and the amount of new bone was increased with time. A large number of bioglass particles exibited a central excabation and bone formation could be observed in the central excabation as well as around the particles. The expression of PDGF was low in the control group. The expression of PDGF in Biocoral group was increased at 1, H week, but decreased at 12 week. The increased PDGF expression in autogenous bone and bioglass group was maintained to the end of the experiment.

• 유체기계공업학회:학술대회논문집
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• 유체기계공업학회 2004년도 유체기계 연구개발 발표회 논문집
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• pp.289-296
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• 2004

The present study has been performed to investigate the influences of rectangular fins on heat transfer in an impingement/effusion cooling system with crossflow. To simulate the impingement/effusion cooling system with initial crossflow, two perforated plates are placed in parallel and staggered arrangements with a gap distance of 2 times of the hole diameter. The crossflow passes between the plates, and various rectangular fins are installed on the plates. Reynolds number based on the hole diameter is fixed to 10,000 and the flow rate of crossflow is changed from 0.5 to 1.5 times of that of the impinging jet. A naphthalene sublimation method is used to obtain the heat/mass transfer coefficients on the effusion plate. Also to analyze the flow characteristics, a numerical calculation is performed. When rectangular fins are installed, the flow and heat transfer pattern is changed greatly from case without fins. In the injection hole region, the jet impinges on effusion plate without deflection and wall jet spreads symmetrically. In the effusion region, the crossflow accelerates due to the decrease of cross-sectional area in the channel. Local heat/mass transfer coefficients are enhanced significantly compared to case without fins. As the blowing ratio increases, the effect of fins against the crossflow becomes more significant and then the higher average heat/mass transfer coefficients are obtained than the case without fins.

• 대한두개안면성형외과학회지
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• 제17권2호
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• pp.63-67
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• 2016

Background: The subciliary approach is commonly used for reconstruction of orbital wall or zygomaticomaxillary fractures. However, this approach is associated with postoperative complications, especially lower eyelid malposition. We report the experience of managing postoperative lower eyelid malposition with triamcinolone acetonide. Methods: A retrospective review was performed for all traumatic facial fractures requiring surgery via the subciliary approach at Chosun University Hospital in 2014. For each patient meeting inclusion criteria, the medical chart was reviewed for demographic information and postoperative course, including the presence of postoperative eyelid malposition or scleral show. Results: The review identified 189 cases in which the subciliary approach was used, and postoperative lower eyelid malposition was found in 7 cases (3.7%). For these 7 patients, the mean therapeutic period (interval to correction of the malposition) was 10.5 weeks (range, 8 to 14 weeks). On average, patients received 3 injections of triamcinolone. In all cases, degrees of the malposition were improved, and none of the patients required an operative intervention to correct the malposition. Conclusion: Triamcinolone injection is an appropriate treatment modality for lower eyelid malposition after subciliary approach. Treatment duration is relatively short, requiring fewer than 4 outpatient clinic visits, with relatively earlier recovery compared to conservative "wait-and-see" management.

• 약학회지
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• 제56권1호
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• pp.20-25
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• 2012

In Northeast Asia, Paeoniae Radix has been used in treatments of inflammation-causing diseases such as arthritis for many centuries. Paeoniflorin, one of the principle bioactive monoterpene glucosides from the paeony root, is reported to be mostly responsible for the effectiveness of the treatments. However, the anti-inflammatory effect of a monoterpene, paeoniflorigenone (PFG) which partially has the moiety of paeoniflorin minus a glucose structure is unknown. Thus, the aim of this work was to investigate anti-inflammatory activity of PFG. For the investigation, PFG activity on the NO (nitric oxide) production from LPS-stimulated macrophages, and the anti-inflammatory effect was tested in the animal model of septic arthritis caused by Candida albicans, a major etiological agent for septic arthritis. For induction of the arthritis, mice were administered with an emulsion of C. albicans cell wall (CACW) mixed with Complete Freund's Adjuvant (CFA) via footpad-injection (Day 0); PFG at a dose of 0.5 or 1 mg/mouse (25 or 50 mg/kg of body-weight) was given to the animals on Day 3, 6, and 9; footpads were scored for arthritis. Moreover, the PFG effect on proliferation of T-lymphocyte that causes aggravation of arthritis was additionally tested. Data resulting from those tests showed that PFG inhibited the NO production from the stimulated macrophage in a dose dependent manner (P<0.05), indicating that PFG is an anti-inflammatory. To confirm the in-vitro results, anti-inflammatory activity of PFG was determined against C. albicans-caused septic arthritis. Data showed that PFG-treatment reduced footpad-swelling which indicates that PFG has anti-arthritic effect (P<0.05), which is therapeutic. The anti-arthritic effect appeared to be mediated by PFG suppression of T-cell proliferation. Ultimately, PFG, a monoterpene component, has anti-inflammatory activity analogous to paeoniflorin. The anti-inflammatory activity treats the septic arthritis due to a pathogenic fungus C. albicans.