• Water Engineering Research
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• 제3권1호
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• pp.23-30
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• 2002

A numerical model for the solution of two-dimensional dam break problems using fractional step method is developed on unstructured grid. The model is based on second-order Weighted Averaged Flux(WAF) scheme with HLLC approximate Riemann solver. To control the nonphysical oscillations associated with second-order accuracy, TVD scheme with SUPERBEE limiter is used. The developed model is verified by comparing the computational solutions with analytic solutions in idealized test cases. Very good agreements have been achieved in the verifications.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2008년도 학술발표회 논문집
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• pp.1752-1756
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• 2008

A simple, accurate and efficient mesh generation technique, the cut-cell method, is able to represent an arbitrarily complex geometry. Both structured and unstructured grid meshes are used in this method. First, the numerical domain is constructed with regular Cartesian grids as a background grid and then the solid boundaries or bodies are cut out of the background Cartesian grids. As a result, some boundary cells can be contained two numerical conditions such as the flow and solid conditions, where the special treatment is needed to simulate such physical characteristics. The HLLC approximate Riemann solver, a Godunov-type finite volume method, is employed to discretize the advection terms in the governing equations. Also, the TVD-WAF method is applied on the Cartesian cut-cell grids to stabilize numerical results. Present method is validated for the rectangular dam break problems. Initially, a conventional grid is constructed with the Cartesian regular mesh only and then applied to the dam-break flow simulation. As a comparative simulation, a cut-cell grids are applied to represent the flow domain rotated with arbitrary angles. Numerical results from this study are compared with the results from the case of the Cartesian regular mesh only. A good agreement is achieved with other numerical results presented in the literature.

• Journal of Microbiology and Biotechnology
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• 제17권3호
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• pp.543-545
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• 2007

4-Hydroxy-3-(3'-methyl-2'-butenyl)-benzoic acid (HMBA) was previously isolated from Curvularia sp. KF119 as a cell-cycle inhibitor. However, the present study used a novel and practical synthetic method to prepare a large quantity of HMBA. The synthetic HMBA was found to inhibit the cell-cycle progression of HeLa cells with a comparable potency to the natural fungal metabolite. The inhibition of the cell-cycle progression by the synthetic HMBA involved both the activation of $p21^{WAF1}$ and the inhibition of cyclin D1 expression in the cells. Consequently, this new synthetic procedure provides an easy and convenient way to produce or manipulate the original fungal metabolite.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2003년도 춘계학술대회 논문집 전기기기 및 에너지변환시스템부문
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• pp.248-252
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• 2003

This paper has a control method proposed for the effective self-tuning fuzzy speed control based on neural network of the induction motor indirect vector control. The vector control of an induction motor provides the decoupled control of the rotor flux magnitude and the torque producing current to performance is desirable. But, the drive performance often degrades for the machine parameter variations and its condition give rise to coupling of flux and torque current. The fuzzy speed control of an induction motor has the robustness about machine parameter variations compared with conventional PID speed control in a way. That proved to be some waf from the true. The purpose of this paper is to improve the adaptation by offering self-turning function to fuzzy speed controller. In this paper, the adaptive mechanism of fuzzy speed control in used ANN(Artificial Neural Network) technique is applied in an IFO induction machine drive, such that the machine can follow a reference model (an ideal field oriented machine) to achieve desired speed. In this paper proved the self-turning method of fuzzy controller has the robustness about parameter variation and the wide range of adaptation by simulation.

• 한국수자원학회논문집
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• 제36권4호
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• pp.597-608
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• 2003

본 연구에서는 천수방정식을 이용하여 2차원 개수로에서 하상과 하폭이 급격히 변화하는 경우에 발생하는 천이류를 해석하였다. 불연속점 근처에서 발생하는 수치진동을 제어하면서 시간과 공간에 대한 2차 정확도를 확보하기 위하여 WAF 기법에 TVD 조건을 갖는 흐름률 제한자를 도입하였으며, Riemann해를 계산하기 위하여 3개의 전파속도를 고려하는 HLLC 방법을 이용하였다. 개수로에서 단면변화를 고려한 2차원 해석을 할 경우, 격자 구성과 경계 처리에서 어려움이 발생한다. 이와 같은 어려움을 해결하기 위하여 일반좌표계를 도입하여 하폭이 변화하는 구간에 발생하는 천이류를 수치모의하였다.

• Water Engineering Research
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• 제3권1호
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• pp.57-62
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• 2002

This research is intended to integrate long-term operation rules and real time operation policy for conservation & flood control in a reservoir. The familiar Yield model has been modified and used to provide long-term rule curves. The model employs linear programming technique under given physical conditions, i.e., total capacity, dead storage, spillways, outlet capacity and their respective elevations to find required and desired minimum storage fur different demands. To investigate the system behavior resulting from the above-mentioned operating policy, i.e., the rule curves, the simulation model was used. Results of the simulation model show that the results of the optimization model are indeed valid. After confirmation of the above mentioned rule curves by the simulation models, gate operation procedure was merged with the long term operation rules to determine the optimum reservoir operating policy. In the gate operation procedure, operating policy in downstream flood plain, i.e., determination of damaging and non-damaging discharges in flood plain, peak floods, which could be routed by reservoir, are determined. Also outflow hydrograph and variations of water surface levels for two known hydrographs are determined. To examine efficiency of the above-mentioned models and their ability in determining the optimum operation policy, Esteghlal reservoir in Iran was analyzed as a case study. A numerical model fur the solution of two-dimensional dam break problems using fractional step method is developed on unstructured grid. The model is based on second-order Weighted Averaged Flux(WAF) scheme with HLLC approximate Riemann solver. To control the nonphysical oscillations associated with second-order accuracy, TVD scheme with SUPERBEE limiter is used. The developed model is verified by comparing the computational solutions with analytic solutions in idealized test cases. Very good agreements have been achieved in the verifications.

• 대한본초학회지
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• 제21권1호
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• pp.33-42
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• 2006

Objectives : The purpose of this study was to investigate the anticancer effects of Bodusan (BDS) on SNU-1 cells, a human gastric cancer cell line. Methods : To study the cytotoxic effect of BDS on SNU-1 cells, the cells were treated with various concentrations of BDS and then cell viability was determined by XTT reduction method and trypan blue exclusion assay. The typical signs of apoptosis, was examined by western blot analysis. BDS-induced MAPK activation was also examined by Western blot for phosphorylated ERK and p38. Results : BDS reduced proliferation of SNU-1 cells in a dose-dependent manner and decreased procaspase 3 level in a dose-dependent manner and induced the clevage of PARP at concentration > 500 ${\mu}g/ml$. BDS also triggered the mitochondrial apoptotic signaling by increasing the release of cytochrome C from mitochondria to cytosol and reducing the level of anti-apoptotic Bcl-2. BDS significantly decreased ERK phosphorylation and increased p38 phosphorylation in a dose-dependent manner. Futhermore, BDS treatment up-regulated p53 and p21waf expression in a dose-dependent manner. Conclusion : BDS-induced apoptosis is MAP kinase-dependent apoptoric pathway and arrested SNU-1 cells at the G0/G1 of cell cycle. These results suggest that BDS is potentially useful as a chemotherapeutic agent in human gastric cancer.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제29권4호
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• pp.199-205
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• 2003

The p53 protein was discovered in 1979 as cellular 53-kD nuclear phosphoprotein bound to the large transforming antigen of SV40 virus. $P21^{WAF1/CIP1}$, which has been described as the critical downstream mediator of p53, is known to suppress DNA replication and arrest the G1 cell cycle by quaternary complex with cyclin D, cyclin-dependent kinase(CDK) and proliferating cell nuclear antigen(PCNA). In these days, some studies shows that the p21 can be induced by independent pathways. There are various reports about the expression of p21 (67%.82.4%) in oral squamous cell carcinoma. But these studies are mostly done in malignant tumor not in benign tumor. So we decided to study the expression of p21 in ameloblastoma and the relationship between p53 and p21 as a downstream mediator of p53 in ameloblastoma. We investigated the expression of p21 and p53 with the method of immunohistochemistry. We selected 30 cases of ameloblastoma tissue blocks (acanthomatous type: 5 cases, follicular type: 8 cases, plexiform type: 17 cases) imbedded in paraffin. We used 30 cases of normal gingival tissues and 30 cases of squamous cell carcinoma tissues (SCC) respectively and compared their results with those of ameloblastoma. We made slides with the streptavidin-biotin methods and used monoclonal antibody DO-7 (Novocastra, Newcastle, United Kingdom) as p53 antibody and monoclonal antibody M7202 (DAKO, California, U.S.A.) as p21 antibody. We used Pearson's correlation coefficient to analyse the relationship. The results were as follows: 1. p21 was expressed in ameloblastoma about 30% and this is lower than that of normal gingiva and SCC. 2. In normal gingiva and ameloblastoma, p21 expression was correlated with p53 expression. 3. In SCC, p21 were expressed about 83.3% and this is more than that of p53. But there was no correlation between p21 and p53 expression. We confirmed p21 expression and relation with p53 in ameloblastoma. But, to confirm the function of p21, more studies about p21 expression in malignant ameloblastoma and ameloblastic carcinoma are needed.

• 한국가축번식학회지
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• 제23권1호
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• pp.13-18
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• 1999

정소내 성숙한 정자를 생산하는 전능성을 가진 정조세포는 체세포와 동일수준으로 외래 유전자를 삽입 가능한 것으로 알려져 있다. 그러나, 이들 세포가 반수체 이후의 단계로 분화한 경우에는 왜래 유전자를 삽입하기보다는 단순히 결합하는 능력이 있는 것으로 알려져 있다 . 따라서, 본 연구는 외래 유전자를 정소실질내 주입함으로써 형질전환 동물생산이 가능한지에 대하여 검토하기 위하여, 한쪽 정소를 거세한 한국 재래산 양을 사용하였다. Liposome /DNA 복합체를 1 : 2의 비율로 희석한 후 정소실실내에 주입하여 정자를 경유한 유전자 전이의 가능성을 확인하였다. 또한 동결보존한 정액을 인공수정하기 위하여 PGF$_2$$\alpha$(0.15mg/kg/BW)를 근육 주사함으로써 인위적으로 발정을 유도한 후, 인공수정을 이용하여 임신과 분만을 유도하였다. 이들 결과를 요약하면 다음과 같다. 1. PCR에 의하여, 유전자 도입 후 채취한 정액에서 외래유전자는 80일 이상 존재하였으며, 가장 높은 전이율은 40 일째 얻어졌다. 이들 결과는 정조세포에 외래 유전자가 성공적으로 삽입되었음을 제안하였다. 2. 23 두 (평균 96 % 의 발정 유기율)의 재래산양에게 인공수정을 실시한 결과 이들 중 4두가 임신되어 7두의 자양이 생산되었다. 3. 생산된 7두의 산자중 genome DNA를 추출하여 PCR 및 Southern blotting을 실시 한 결과 2두가 형질전환으로 확인되었다. 이상의 결과로서 정자를 매개로 한 정소실질내 외래 유전자의 주입법은 형질전환의 생산을 위한 매우 유용한 수단으로 사용 가능함을 제안하였다.

• Tuberculosis and Respiratory Diseases
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• 제57권5호
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• pp.449-460
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• 2004

연구배경 : PS-341은 최근에 개발된 강력하고 특이적인 proteasome 억제제로서, 일부 암환자에 투여하여 좋은 성적이 보고되고 있다. Proteasome 억제제의 항암효과는 아포프토시스 유발 물질, 즉 p53, $p21^{WAF/CIP1}$, $p27^{KIP1}$, NF-${\kappa}B$, Bax, Bcl-2 등의 발현 증가와 관련이 있는 것으로 생각되고 있다. JNK와 GSK-$3{\beta}$도 아포프토시스에 관여하는 것으로 잘 알려져 있지만, PS-341에 의한 아포프토시스에서의 역할은 규명되지 못한 상태이다. 본 연구에서는 폐암세포주에서 PS-341에 의한 아포프토시스에서 JNK와 GSK-$3{\beta}$의 역할을 규명하고자 하였다. 방 법 : NCI-H157과 A549 폐암세포주를 실험에 사용하였다. 세포생존능은 MTT 방법으로 평가하였고, 아포프토시스는 PARP의 분해로 평가하였다. JNK의 활성도는 in vitro immuno complex kinase 방법과 내인성 c-Jun의 인산화로 측정하였다. 각종 단백의 발현은 Western 분석으로 평가하였다. JNK1과 GSK-$3{\beta}$의 과발현은 각각 plasmid vector와 adenovirus vector를 이용하였다. 결 과 : PS-341 처치로 아포프토시스에 의한 세포생존율의 감소가 관찰되었다. PS-341 처치로 JNK가 활성화되었고, c-Jun의 발현이 유도되었다. Dominant negative JNK1의 과발현 또는 SP600125 전치치로 JNK의 활성화를 차단하면 PS-341에 의한 아포프토시스가 억제되었다. PS-341 처리로 JNK 활성화에 의존적으로 caspase 3의 활성화가 유도되었다. Caspase 활성화의 차단으로도 PS-341에 의한 아포프토시스가 억제되었다. PS-341에 의해 Akt가 활성화되었고, Akt 활성화의 차단으로 PS-341에 의한 아포프토시스가 심화되었다. PS-341에 의한 Akt 활성화로 GSK-$3{\beta}$가 불활성화되었다. Constitutively active GSK-$3{\beta}$의 과발현으로 PS-341에 의한 아포프토시스가 심화되었고, dominant negative GSK-$3{\beta}$의 과발현으로 PS-341에 의한 아포프토시스가 감소되었다. Lithium chloride 전처치와 dominant negative GSK-$3{\beta}$의 과발현으로 PS-341에 의한 JNK의 활성화와 c-Jun의 발현 증가가 억제되었다. 결 론 : 폐암세포주에서 PS-341에 의한 아포프토시스는 JNK/caspase 경로가 관여하며, 이는 PI3K/Akt 경로를 통한 GSK-$3{\beta}$의 불활성화에 의해 억제되는 것으로 판단된다. 따라서 PS-341의 항암효과를 최대화하기 위해서는 PI3K/Akt 경로를 통한 GSK-$3{\beta}$의 불활성화를 차단하는 치료법이 병행되어야 할 것으로 판단된다.