• Toxicological Research
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• 제28권1호
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• pp.57-65
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• 2012

In this study, the 4-week oral toxicity and anti-cancer activity of the hexane layer of Melia azedarach L. var. japonica Makino's bark extract were investigated. We carried out a hollow fiber (HF) assay and 28-day repeated toxicity study to confirm the anti-cancer effect and safety of the hexane layer. The HF assay was carried out using an A549 human adenocarcinoma cell via intraperitoneal (IP) site with or without cisplatin. In the result, the 200 mg/kg b.w of hexane layer with 4 mg/kg b.w of cisplatin treated group, showed the highest cytotoxicity aginst A549 carcinoma cells. For the 28-day repeated toxicity study, 6 groups of 10 male and female mice were given by gavage 200, 100, or 50 mg/kg b.w hexane layer with or without 4 mg/kg b.w of cisplatin against body weight, and were then sacrificed for blood and tissue sampling. The subacute oral toxicity study in mice with doses of 200, 100, and 50 mg/kg b.w hexane layer showed no significant changes in body weight gain and general behavior. The cisplatin-treated group significantly decreased in body weight compared to the control group but regained weight with 100 and 200 mg/kg b.w of hexane layer. The biochemical analysis showed significant increase in several parameters (ALT, total billirubin, AST, creatinine, and BUN) in cisplatin-treated groups. However, in the group given a co-treatment of hexane layer (200 mg/kg b.w), levels of these parameters decreased. In hematological analysis, cisplatin induced the reduction of WBCs and neutrophils but co-treatment with hexane layer (100 and 200 mg/kg b.w) improved these toxicities caused by cisplatin. The histological profile of the livers showed eosinophilic cell foci in central vein and portal triad in cisplatin treated mice. These results show that hexane layer might have an anti-cancer activity and could improve the toxicity of cisplatin.

• 한국수산과학회지
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• 제35권6호
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• pp.686-695
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• 2002

Samples of Corbicula ( Corbicula) japonica Prime of Namdae Stream in Gangnung were collected from November 2000 to October 2001. Age of C. (C.) japonica was determined from the rings on the shell, The shell length of the samples ranged from 8 mm to 38 mm. The ring on the shell was formed once a year in March. Von Bertalanffy's growth parameters were estimated using a nonlinear regression method, asyinptotie shell length ($L_{\omega}$) was 48,98 mm, K was 0.20421year, theoretical age at 0 shell length $(t_0)$ was 0.3169 year, and asymptotic total weight ($W_{\omega}$) was 41.37 g. The formula of allomeky between shell length (L, mm) and total weight (W, g) of the brackish water clam was W=3.42$\times$10^{-4}L^{3}. The annual survival rate was estimated at 0.3799, instantaneous coefficient of natural mortality was 0.5007/year, and instantaneous coefficient of fishing mortality was 0.46721year. The age at first capture was estimated at 2.1593 year using shell length compositions of the brackish water clam, The current yield-per-recruit at 0.4672/year of fishing mortality was 0.6595 g. F_0.1 was estimated at 0.1865/year, Acceptable biological catch was estimated at 14.4 metric ton.

• 한국자원식물학회지
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• 제31권1호
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• pp.16-23
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• 2018

백작약 물 추출물과 유기용매 분획물들의 항산화 활성, 트롬빈 억제 및 구강암세포주에서의 암전이 억제 및 암세포 사멸능을 확인하였다. 에틸아세테이트 분획물이 85.13%의 높은 항산화 활성과 87.54%의 높은 트롬빈저해 효과를 나타냈으며, 또한 구강암세포주에서도 트롬빈 처리에 의해 활성화된 MMP-2/pro-MMP-2이 높은 암전이 억제 활성을 나타냈다. 그리고 구강암세포주에 대한 세포사멸 효과는 물 추출물이 5배 이상의 높은 능력을 보였다. 이와 같은 연구 결과를 바탕으로 백작약 에틸아세테이트 분획물은 새로운 항산화제, 트롬빈억제제 및 암전이억제제의 개발을 위한 우수한 천연물 소재 후보 물질로서의 가능성을 제시하고 있다.

• 대한예방한의학회지
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• 제12권1호
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• pp.119-126
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• 2008

This study was carried out to investigate the anti-oxidant effect of Wasabia japonica(WJ). The anti-oxidant activities of various extracts from WJ were investigated. The water and ethanol extracts of WJ leaf were found to cause significant free radical scavenging effects on DPPH and nitric oxide(NO). Sprague-Dawley male rats were randomly assigned to one normal diet and three high cholesterol diet groups which contained 1%(w/w) cholesterol. After 4weeks cholesterol diet, the groups of high cholesterol diet were classified to control(normal diet only), WJR5(normal diet and 5% WJ root) and WJL5(normal diet and 5% WJ leaf). Liver xanthine oxidase(XO) activity in the rats of high cholesterol diet was decreased up to the levels of normal diet group according to WJ diet. The results indicated that WJ possesses antioxidant effects through free radical scavenging effects on DPPH, NO and the decreasing of XO activity. In view of these results, WJ is expected to be an effective material for the anti-oxidant.

• The Korean Journal of Ecology
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• 제27권1호
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• pp.27-33
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• 2004

Cryptomeria japonica plantation was monitored every year during 15 years from 1983 to 1997 for stem diameter and volume. The reciprocal equation, 1/Y = A + B/N, was applied to the relationship between cumulative volume Y and cumulative number N from the largest tree in the stand each year. The parameters A and B, which means respectively the reciprocal of an asymptotic value of total stand stem volume and the reciprocal of the maximum stem volume, are related by a power function. The power functional relationship between A and B derived a linear relationship of B-points ( $N_{B}$, $V_{B}$; $N_{B}$ = B/A, $Y_{B}$ = 1/2A) of each Y-N curve on log-log coordinates. The gradient of B-point line was so steep that the Y-N curve moved parallel upward year by year. The time trajectory of mean stem volume (W) and density ($\rho$) provided evidence in favor of the 3/2 power law of self-thinning, because the gradient of W - $\rho$ trajectory on log-log coordinates approximated to -3/2 at the final stage of stand development. On the basis of the results of Y-N curves and W - $\rho$ trajectory, the time trajectory of maximum stem volume $W_{max obs}$ and $\rho$ was derived theoretically. The gradient of $W_{max obs}$ - $\rho$ trajectory on log-log coordinates is calculated to be -0.6105 at the final stage. The gradient of $W_{max obs}$ - $\rho$ trajectory was steeper than that of W - $\rho$ trajectory at the early stage, while the former is gentler than the latter at the later stage.stage.e.age.e.

• 대한본초학회지
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• 제25권4호
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• pp.129-135
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• 2010

Objectives : The present study was undertaken to determine whether the water extract of Achyranthis Radix, which is the roots of Achyranthes japonica (Achyranthis Radix, AR), is efficacious against collagen-induced arthritis (CIA) in DBA/1J mice. Methods : Mice were immunized with bovine type II collagen and orally treated with AR-W (50 and 100 mg/kg/bw) from days 21 to 42 after immunization. Arthritis was evaluated by arthritic score, histological examination of knee joint and serological markers such as TNF-${\alpha}$, $PGE_2$ and anti-type II collagen (C II)IgG. Results : The results showed that comparing with untreated CIA mice, treated with AR-W significantly suppressed the clinical score and joint tissue pathological damages, reduced the serum levels of TNF-${\alpha}$, $PGE_2$ and anti-C II IgG in CIA-mice. These results suggest that AR-W can effectively alleviate inflammatory response on CIA, and anti-inflammatoy of AR-W can be attributed, at least partially, to the inhibition of inflamamtory mediators, $PGE_2$ and TNF-${\alpha}$, in CIA. Conclusions : This study suggests that AR-W has a therapeutic potential in inflammatory joint diseases such as rheumatoid arthritis.

• KSBB Journal
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• 제27권2호
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• pp.86-90
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• 2012

Ethanol fermentations were carried out using simultaneous saccharification and fermentation (SSF) and separated hydrolysis and fermentation (SHF) processes with monosaccharides from seaweed, Saccharina japonica (sea tangle, Dasima) as the biomass. The pretreatment was carried out by thermal acid hydrolysis with $H_2SO_4$ or HCl. Optimal pretreatment condition was determined at 10% (w/v) seaweed slurry with 37.5 mM $H_2SO_4$ at $121^{\circ}C$ for 60 min. To increase the yield of saccharfication, isolated marine bacteria Bacillus sp. JS-1 was used and 48 g/L of reducing sugar were produced. Ethanol fermentation was performed using SSF and SHF process with Pachysolen tannophilus KCTC 7937. The ethanol concentration was 6.5 g/L by SSF and 6.0 g/L by SHF.

• 한국가금학회지
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• 제17권4호
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• pp.269-274
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• 1990

일본산메추리(Coturnix coturnix japonica)의 핵형연구를 위하여 정소조직 및 백혈구, 초기태아조직들로서 염색체 분석을 수행하였다. 이들 염색체의 수는 정상 이배체(2n)가 78개로 나타나고, 계2 감수분열 중기상태의 정모세포의 관찰에서 반수체(n)가 39개로 나타났다. 한편 8개의 대형염색체 및 성 염상체에 대한 형태적 분석은 동원체지수 및 상대적 길이비로서 측정하였다. 이용된 여러 조직간의 염색체의 형태적 차이는 거의 없는 것으로 나타났으나 4번 염색체템서 p-alm의 존재유무에 따라 상당한 변이를 나타내었다.

• Preventive Nutrition and Food Science
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• 제15권2호
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• pp.147-151
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• 2010

The volatile aroma constituents of Atractylodes japonica rhizome were separated by steam distillation extraction method using a Clevenger-type apparatus, and analyzed by gas chromatography-mass spectrometry (GC/MS). The yield of the essential oil from Atractylodes japonica was 1.0% (v/w), and its color was pale yellow. Forty-five volatile flavor compounds, which make up 93.86% of the total peak area, were tentatively identified in the rhizome oil. The oil contained 32 hydrocarbons (79.19%) with sesquiterpene hydrocarbon predominating, 3 esters (12.46%), 4 alcohols (0.11%), 1 ketone (0.01%), 2 aldehydes (0.02%), and 3 miscellaneous compounds (2.07%).

• 생약학회지
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• 제43권2호
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• pp.98-100
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• 2012

Korean folk medicine 'DeungNaMu' has been used mainly to cure apoplexy, pain of the stapes and tonic for weak man. With regard to the botanical origin of DeungNaMu, it has been considered to be Wisteria species of Leguminosae, but there was no pharmacognostical confirmation on it. To clarify the botanical origin of 'DeungNaMu', the anatomical characteristics of the branches of Wisteria species growing wild in Korea, Wisteria japonica and W. japonica forma. alba were studied. As a result, it was clarified that DeungNaMu was the branches of Wisteria japonica.