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http://dx.doi.org/10.7732/kjpr.2018.31.1.016

Evaluation of Anti-oxidative, Anti-thrombin, Anti-invasive and Pro-apoptotic Activities of Paeonia japonica  

Kim, Jun-Ho (Department of Fine Chemistry and New Materials, Sangji University)
Kim, Eun-Jung (Department of Biomedical Laboratory Science, Sangji University)
Publication Information
Korean Journal of Plant Resources / v.31, no.1, 2018 , pp. 16-23 More about this Journal
Abstract
Paeonia japonica is a perennial flowering plant used in traditional medicine therapy. The purpose of this study was to investigate the effect of water extract and solvent fractions obtained from P. japonica on anti-oxidative, anti-thrombin, anti-invasive and pro-apoptotic activities in YD-10B cells, human oral squamous carcinoma cell line. Water fraction revealed the highest extraction yield at 11.44% (w/w). Anti-oxidative activity was the highest in ethyl acetate fraction (85.13%). In the thrombin inhibitory activity test, ethyl fraction was the highest, with a value of 87.54%. Release and activation of MMP-2/pro-MMP-2 ratio in thrombin-treated YD-10B cells were significantly inhibited in the ethyl acetate fraction. At a concentration of $120{\mu}g/m{\ell}$ water extract and solvent fractions of P. japonica inhibited cell proliferation in YD-10B cells except water fraction. Pro-apoptotic effect on human oral squamous carcinoma cell using the Bax/Bcl-2 ratio analysis was higher in water extract than other fractions. These findings suggest that the ethyl acetate fraction of P. japonica potentiates a promising antioxidant, anti-thrombin and anti-invasive agents.
Keywords
Anti-oxidative activity; Anti-thrombin activity; Anti-invasive activity; Paeonia japonica; Pro-apoptotic activity;
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