• Journal of Physiology & Pathology in Korean Medicine
• /
• v.30 no.4
• /
• pp.250-256
• /
• 2016

The prevalence of renal disease is increased with the overweight and obesity. High fat diet-associated oxidative stress increases production of reactive oxygen species (ROS) and induces apoptosis. There are two types of antioxidant defense mechanisms for oxidative stress. One is the enzyme defense mechanism by antioxidant enzymes such as superoxide dismutase (SOD), and catalase (CAT). The other is non-enzyme defense mechanism by signaling molecules such as nuclear factor-like 2 (Nrf-2), HO-1. In this study, we induced obesity in mice with high fat diet for six weeks and thereafter administered orally Viola mandshurica for 4 weeks. V. mandshurica is known to clear heat, detoxify and cool blood, and subside a swelling effect. In the V. mandshurica administered group, the immunoreactive signal of the Tunel staining was weaker than that of obesity group. Proapoptotic Bax, caspase 3 immunoreactives of the V. mandshurica administered group was lower than those of obesity group, whereas anti-apoptotic Bcl-2 immunoreactity was higher in the V. mandshurica administered group. Antioxidant enzyme mechanism such as superoxide dismutase 2 (SOD2), catalase (CAT) immunoreactives of the V. mandshurica administered group and Antioxidant non-enzyme mechanism such as Nuclear factor-like 2 (Nrf2), Heme Oxygenase 1 (HO-1) immunoreactives of the V. mandshurica administered group was higher than those of obesity group. These results demonstrate that V. mandshurica had the antioxidant and anti-apoptosis effects on obese mice.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.37 no.9
• /
• pp.1101-1108
• /
• 2008

This study was carried out to compare the physiological activities of the extracts from Korean and Chinese Viola mandshurica W. Baker. The water extract from leaves of Chinese V. mandshurica exhibited the highest extraction yields of 30.45 g/100 g and the highest content of total flavonoids as 102.30 mg/g. Also, its ethanol extract showed the best content of polyphenol compounds as 136.16 mg/g. The leaf extract of Korean V. mandshurica produced higher electron donating abilities (EDA) of 92.69% (KLW) and 93.61% (KLE) than the other fractions. The strongest SOD-like activity was shown in the ethanol extract from Korean leaves of 17.28% at the concentration of 1.0 mg/mL. The nitrite scavenging abilities (NSA) of the leaf extracts of V. mandshurica from China were intense over 50% at pH 1.2 and 3.0. In the results of inhibitory rates of xanthine oxidase (XO), both ethanol extracts from Korean and Chinese leaves were higher than the other fractions as 98.67% and 93.80% respectively. Effect of tyrosinase inhibition was the highest in the water extract (45.04%) of Chinese leaves, followed by its ethanol extract (31.36%). The results of EDA, SOD-like activity and XO inhibition of the leaf extracts from Korean V. mandshurica were higher than those of Chinese, on the other hand, determinations on total polyphenol contents, NSA and tyrosinase inhibition were higher in those of Chinese.

• Korean Journal of Pharmacognosy
• /
• v.41 no.3
• /
• pp.166-173
• /
• 2010

Oxidative stress to proteins, lipids, or DNA is higher in human autopsy tissue and in rodent models of a number of neurodegenerative conditions, including Alzheimer's and Parkinson's disease. On the basis of this information, we established a screening system using N18-RE-105 cells to identify therapeutic agents that can protect cells from glutamate toxicity. During the course of our screening program, we recently isolated the active compound 9-hydroxy-$\alpha$-tocopherone ($\alpha$-TP), which prevents glutamate-induced cell death, from Viola mandshurica. The chemical structure of $\alpha$-TP was identified using spectroscopic methods and by comparison with literature values. Antioxidant activity and protective effects of $\alpha$-TP were evaluated by DPPH radical-scavenging assay, morphological assay, MTT reduction assay, and lactate dehydrogenase (LDH) release assay. These results suggest that $\alpha$-TP could be a new potential chemotherapeutic agent against neuronal diseases.

• Applied Biological Chemistry
• /
• v.51 no.1
• /
• pp.79-83
• /
• 2008

The present study describes glutamate which is known as excitatory neurotransmitter is related with oxidative damages and the Viola mandshurica extracts. Showed protective effects against glutamate-induced cytotoxicity. The protective effect of antioxidant on the glutamate treated N18-RE-I05 cells was determined by a MTT reduction assay. The neuroprotective effect of methanol, ethanol, and acetone extracts from V. mandshurica against glutamate-induced cytotoxicity was assessed by the results of an MTT reduction assay. Among the three extracts, the acetone extract showed the highest protective effect by the results of an lactate dehydrogenase release assay. Therefore, these results suggest that V. mandshurica extracts could be a new potential candidate against glutamate-induced oxidative stress.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.37 no.4
• /
• pp.405-409
• /
• 2008

This study was performed to investigate the physiological activities of Viola mandshurica. Antioxidant activity was evaluated by measuring total phenolic contents, reducing power, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-di-2-ethyl-benzthiazoline sulphonate (ABTS) radical scavenging activity while anti-diabetic activity was measured by inhibition activities on ${\alpha}$-amylase and ${\alpha}$-glucosidase. V. mandshurica extracts were prepared by extracting with four different solvents (methanol, ethanol, acetone, and water). The methanol extract showed the highest total phenol content (34.49 mg/g gallic acid equivalents) among the extracts. The water extract showed the highest reducing power (0.454) at the concentration of $1,000{\mu}g$/mL. The acetone extract showed the most potent radical scavenging activity. DPPH and ABTS radical scavenging activity of the acetone extract at the concentration of $1,000{\mu}g$/mL were 21.13% and 43.53%, respectively. The inhibitory activity of acetone extracts against ${\alpha}$-amylase and ${\alpha}$-glucosidase showed more than 100% at the concentration of $1,000{\mu}g$/mL. The results indicate that V. mandshurica might have potential antioxidant and anti-diabetic activities.

• Food Science and Preservation
• /
• v.15 no.5
• /
• pp.699-705
• /
• 2008

This study compared amino acids and effective components of Viola mandshurica produced in Korea with those of Chinese plants, as part of a study on nutritional andfunctional materials, to aid in the development of a valuable food. The contents of reducing sugar, free sugar, polyphenols, and soluble protein in the flowers of V. mandshurica were higher than in other fractions, being 10,485.11 mg%, 118.85 mg%, 1,259.26 mg%, and 23.67 mg%, respectively. Total flavonoid content of Chinese leaves was the highest at 2,370.75 mg%, followed by 1,844.43 mg% in Korean leaves. Free amino acids were highest in Korean leaves (5,345.21 mg%), followed by roots (4,692.24 mg%), flowers (3,807.87 mg%), and leaves of China plants (3,613.81 mg%). Arginine accounted for 55% of free amino acids in the roots, and all plant fractions contained more valine, aspartic acid, glutamic acid, alanine, and arginine, than other amino acids. The total content of amino acid derivatives was the highest (9,058.80 mg%) in Korean leaves. Especially, $\alpha$-aminoadipic acid constituted over 50% of total amino acid derivatives in all plant parts of V. mandshurica. When mineral contents were analyzed, leaves grown in China showed the highest content of 5,917.57 mg%, and the contents of K, Ca, and Mg were much greater than those of other minerals, together comprising over 95% of total mineral content in leaves and flowers from Korea.

• Journal of Plant Biology
• /
• v.23 no.3_4
• /
• pp.85-90
• /
• 1980

The chemical affinities among 12 species of Viola from Jeju Island are investigated by thin-layer chromatography for their MeOH soluble and aglycon parts. They are classified into three groups which are closely related to those of morphological classification: the first allience-Viola hirtipes, V. chaerophylloides, V. meta-japonica, V. keiskei var. okuboi and V. violacea; the second allience-V. xanthopetala, V. ovata-oblonga, V. acuminata var. acuminata and V. grypoceras; and the third allience-V. pachyrhiza, V. phalacrocarpa and V. mandshurica var. ciliata. The first allience consists of the species without stem which have PA value greater than 50% with one another. V. hirtipes and V. chaerophylloides show closer affinity among the taxa of this allience. The second allience consists of the species with stem, of which PA value are about 50%. The third allience consists of the species without stem which have PA values lower than 50% when compared with any other taxa.

• Korean Journal of Plant Taxonomy
• /
• v.51 no.2
• /
• pp.166-170
• /
• 2021

Distribution of Viola inconspicua is newly discovered in Korea. This species was collected from grassy fields and roadsides in Jeju-si and Seogwipo-si, Jejudo Island. V. inconspicua shares several characteristics (i.e., purple or pale purple flowers, triangular leaf blades, winged petioles, and a linear-lanceolate stipules) with the related species V. mandshurica and V. yedoensis. However, V. inconspicua can be easily distinguished from other species by the following characteristics: leaf base (cordate vs. truncate to attenuate), and spur (short, 2-4 mm long vs. longer, 5-8 mm long). Here, we describe and illustrate of V. inconspicua. In addition, the identification key to allied species, photographs in its habitat, distribution, ecology, and phenology are provided as well. The Korean name for the species is given as 'Hwa-sal-ip-je-bi-kkot', considering the shape of its leaves.

• Korean Journal of Plant Resources
• /
• v.19 no.6
• /
• pp.700-705
• /
• 2006

Present studies were performed to determine the physiology of seed germination in Viola species native to Korea. Twelve species, 1 variety and 1 form were collected, classified and used as materials: V. collina, V. blandaefomis, V. rosii, V. chaerophylloides, V. phalacrocarpa, V. patrinii, V. mandshurica, V. mandshurica for. albescence, V. seoulensis, V. yedoensis, V. keiskei, V. variegata, V. variegata var. chinensis, and V. verecunda. V. tricolor 'Helen Mount' was also used to compare wild with cultivated species. In order to investigate the effect of temperature on seed germination, seeds stored at $4{\pm}2^{\circ}C$ for 10 months or 4 years were incubated at 10, 15, 20, $25^{\circ}C$ under 16h illumination with 4 replicates per treatment. Seeds which had not germinated at $10^{\circ}C$ were transferred to $30^{\circ}C$ to assess the effect of temperature change in germination. Germination percent and the days of first, 40% and 80% germination were assessed. Capability of seed germination varied with taxon; Species belonging to subsection Patellares had high ability of germination, compared to species in the other subsections, and series Chinensis was the best among subsection Patellares. Species capable of high germination germinated in all temperatures with reasonably high germination rate, but the other species responded sensitively to temperature with different germination patterns. Higher the temperature, shorter the incubation time required for first, 40% and 80% of germination. Therefore, high temperature was effective in almost all species, not only for inducing high rate of germination but also the uniformity of germination. Temperature change from $10^{\circ}C\;to\;30^{\circ}C$ had a positive effect on seed germination.

• Journal of the Korean Applied Science and Technology
• /
• v.28 no.1
• /
• pp.75-84
• /
• 2011

Atopic dermatitis is a chronic inflammatory skin disease associated with cutaneous hyper-reactivity to environmental triggers. In order to develop effective therapeutic herbal extracts for atopic dermatitis, cytotoxicity, antioxidant, anti-inflammatory and anti-allergic activities were investigated for various herbal extracts. Among candidate extracts, we selected Aloe vera L. (AV), Viola mandshurica W. Becker (VM), Punica granatum L. (PG), Dendrobium nobile L. (DN) and mixture of the above extracts (MX) for further investigations. All of them did not show cytotoxic activities to macrophage RAW264.7 cells below the concentration of 100 ppm. All showed antioxidant, anti-inflammatory and anti-allergic effects, although to various extents. In antioxidant effects, AV showed the highest effect, followed by PG and VM, while DN did the lowest. In evaluation for anti-inflammatory activities in macrophage RAW264.7 cells, AV and DN inhibited almost completely the LPS-induced production of nitric oxide, while AV, DN and VM showed strong inhibitory activities on the LPS-induced production of TNF-${\alpha}$. In anti-allergy effect in mast cell HMC-1, DN showed the highest effect, followed by AV and PG, while VM did the lowest. In the topical allergy reaction induced by compound 48/80 in Sprague-Dawley rat, DN exhibited significant anti-allergic effect, while PG, VM and AV did slight effect. These results suggest that AV, VM, PG and DN have antioxidant, anti-inflammatory and anti-allergic activities, and thus have the potential to reduce and alleviate the symptoms of atopic dermatitis.