Browse > Article
http://dx.doi.org/10.15188/kjopp.2016.08.30.4.250

Antioxidant Effect of Viola mandshurica W. Becker on the High Fat Diet-Induced Renal Oxidative Stress  

Choi, Mi Hye (Department of Anatomy, College of Korea Medicine, Dongguk University)
Park, In Sik (Department of Anatomy, College of Korea Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.30, no.4, 2016 , pp. 250-256 More about this Journal
Abstract
The prevalence of renal disease is increased with the overweight and obesity. High fat diet-associated oxidative stress increases production of reactive oxygen species (ROS) and induces apoptosis. There are two types of antioxidant defense mechanisms for oxidative stress. One is the enzyme defense mechanism by antioxidant enzymes such as superoxide dismutase (SOD), and catalase (CAT). The other is non-enzyme defense mechanism by signaling molecules such as nuclear factor-like 2 (Nrf-2), HO-1. In this study, we induced obesity in mice with high fat diet for six weeks and thereafter administered orally Viola mandshurica for 4 weeks. V. mandshurica is known to clear heat, detoxify and cool blood, and subside a swelling effect. In the V. mandshurica administered group, the immunoreactive signal of the Tunel staining was weaker than that of obesity group. Proapoptotic Bax, caspase 3 immunoreactives of the V. mandshurica administered group was lower than those of obesity group, whereas anti-apoptotic Bcl-2 immunoreactity was higher in the V. mandshurica administered group. Antioxidant enzyme mechanism such as superoxide dismutase 2 (SOD2), catalase (CAT) immunoreactives of the V. mandshurica administered group and Antioxidant non-enzyme mechanism such as Nuclear factor-like 2 (Nrf2), Heme Oxygenase 1 (HO-1) immunoreactives of the V. mandshurica administered group was higher than those of obesity group. These results demonstrate that V. mandshurica had the antioxidant and anti-apoptosis effects on obese mice.
Keywords
Viola mandshurica W. Becker; Antioxidant effect; Apoptosis; High fat diet;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Ko, K.S. A study on Antioxidant Effect of Methanol Extract from Viola Mandshurica. Journal of the korean socity of Cosmetology. 18(5):1082-1086, 2012.
2 Lee, M.R., Hwang, J.H., Park, J.H., Kim, H.J., Park, E.J., Park, H.R. Antioxidant Activity and Protective Effects of 9-hydroxy-α-tocopherone from Viola mandshurica Extracts. Kor. J. Pharmacogn. 41(3):166-173, 2010.
3 Jeon, G.I., Yoon, M.Y., Park, H.R., Lee, S.C., Park, E.J.. Neuroprotective Activity of Viola mandshurica Extracts on Hydrogen Peroxide-Induced DNA Damage and Cell Death in PC12 Cells. Ann. N.Y. Acad. Sci. 1171: 576-582, 2009.   DOI
4 Lee, M.Y., Yuk, J.E., Kwon, O.K., Kim, H.S., Oh, S.R., Lee, H.K., Ahn, K.S. Anti-inflammatory and anti-asthmatic effects of Viola mandshurica W. Becker(VM) ethanolic (EtOH) extract on airway inflammation in a mouse model of allergic asthma. Journal of Ethnopharmacology. 127: 159-164, 2010.   DOI
5 Lee, B.U. Inhibitory effect of Violae herba extract on inflammatory cytokine production induced by IL-1β and TNF-α in culured human synovial cells. Dept. of Oriental Pathology, College of Traditional Korea med., Daejeon Univ. 2003.
6 Ramesh, G., Brian Reeves, W. Cisplatin increases TNF-alpha mRNA stability in kidney proximal tubule cells. Ren Fail. 28: 583-592, 2006.   DOI
7 Kondoh, M., Araragi, S., Sato, K., Higashimoto, M., Takiguchi, M., Sato, M. Cadmium induces apoptosis partly via caspase-9 activation in HL-60cells. Toxicology. 170(1-2):111-117, 2002.   DOI
8 Sang Won Kang. Role of Reactive Oxygen Species in Cell Death Pathways. Hanyang Med Rev. 2013;33:77-82   DOI
9 Park, J.Y., Kim, D.M., Kim, B.W., Kim, Y.K., Kim, Y.J., Kim, T.H., Yoo, H.J., Lee, M.K., Lee, Y.K., Cha, B.Y., Ha, S.W. Pathogenic Mechanisms of Hypertension and Kidney Disease in Metabolic Syndrome. Biological Research Information Center, BioWave. 1: 1-11, 2002.
10 Big data streering Dept. Health Insurance Big Data Analytics for 12 years 1 million case. Extremely obese increased more than four times in 20-30 age. Korea's first trend analysis about extremely obese per year. National Health Insurance Service. [cited 2014 Sep 15]; URL:http://www.nhis.or.kr/bbs7/boards/B0039/3164
11 Centers for Disease Control & Prevention. 2013 National health statistics. Ministry of Health and Welfare, Centers for Disease Control & Prevention. 6(1):50-51, 2014.
12 Bae, G.G., Ha, B.J. Antioxidative effect of Fermented Rhynchosia nulubilis in Obese Rats. J. Food Hyg. Saf. 30(4):383-389, 2015.   DOI
13 Lee, Y.S., Kim. N.W. Comparative Analysis of the Amino Acids and Effective Components from Viola mandshurica. Korean J. Food Preserv. 15(5):699-705, 2008.
14 Lee, B.B., Park, S.R., Suk Han, C.S., Han, D.Y., Park, E.J., Park, H.R., Lee, S.C. Antioxidant Activity and Inhibition Activity against α-Amylase and α-Glucosidase of Viola mandshurica Extracts. J Korean Soc Food Sci Nutr. 37(4):405-409, 2008.   DOI
15 Sin, M.G. Clinical Traditional Herbalogy in color. Seoul:Namsandang. p 340, 1986.
16 Lee S.Z. Ben Cao Gang Mu(Compendium of Materia Medica). Seoul:Gomunsa. p 647, 1983.
17 Sung, Y.Y., Kim, D.S., Kim, H.K. Viola mandshurica ethanolic extract prevents high-fat-diet-induced obesity in mice by activating AMP-activated protein kinase. environmental toxicology and pharmacology. 38: 41-50, 2014.   DOI
18 Park, S.W., Seo, H.S., Kim, J.W., Hong, S.J., Shin, S.H., Rha, S.W., Ahn, J.C., Song, W.H., Park, C.G., Shim, W.J., Oh, D.J., Ro. Y.M. Effect of Human Bcl-2 Gene Expression on the Peripheral Atherosclerotic Lesions of Apolipoprotein E-Deficient Mouse. Korean Circulation J. 35: 725-733, 2005.   DOI
19 Alonso-Galicia, M., Dwyer, T.M., Herrera, G.A., Hall, J.E. Increased hyaluronic acid in the inner renal medulla of obese dogs. Hypertension. 25: 888-892, 1995.   DOI
20 Kim, S.H., Han, Y.J., Park, J.H., Yoo, S.J. Glutamate Induces Endoplasmic Reticulum Stress-Mediated Apoptosis in Primary Rat Astrocytes. J Korean Geriatr Soc. 14(4):242-252, 2010.   DOI
21 Choi, J.Y., Kim, K.H., Choi, J.Y., Han, C.W., Ha, K.T., Jeong, H.S., Joo M.S. Identification of the Plant Part of Gleditsia sinensis that Activates Nrf2, an Anti-oxidative Transcription Factor. Korean J. Oriental Physiology & Pathology. 28(3):303-309, 2014.
22 Turpaev, K.T. Keap1-Nrf2 signaling pathway : mechanisms of regulation and role in protection of cells against toxicity caused by xenobiotics and electrophiles. Biochemistry. 78(2):111-126, 2013.