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Antioxidant Activity and Protective Effects of 9-hydroxy-$\alpha$-tocopherone from Viola mandshurica Extracts  

Lee, Mi-Ra (Department of Food Science and Biotechnology, Kyungnam University)
Hwang, Ji-Hwan (National Institute of Advanced Industrial Science and Technology (AIST))
Park, Jae-Hee (Department of Food and Nutrition, Kyungnam University)
Kim, Hyun-Jung (Department of Food Science and Biotechnology, Kyungnam University)
Park, Eun-Ju (Department of Food and Nutrition, Kyungnam University)
Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
Publication Information
Korean Journal of Pharmacognosy / v.41, no.3, 2010 , pp. 166-173 More about this Journal
Abstract
Oxidative stress to proteins, lipids, or DNA is higher in human autopsy tissue and in rodent models of a number of neurodegenerative conditions, including Alzheimer's and Parkinson's disease. On the basis of this information, we established a screening system using N18-RE-105 cells to identify therapeutic agents that can protect cells from glutamate toxicity. During the course of our screening program, we recently isolated the active compound 9-hydroxy-$\alpha$-tocopherone ($\alpha$-TP), which prevents glutamate-induced cell death, from Viola mandshurica. The chemical structure of $\alpha$-TP was identified using spectroscopic methods and by comparison with literature values. Antioxidant activity and protective effects of $\alpha$-TP were evaluated by DPPH radical-scavenging assay, morphological assay, MTT reduction assay, and lactate dehydrogenase (LDH) release assay. These results suggest that $\alpha$-TP could be a new potential chemotherapeutic agent against neuronal diseases.
Keywords
9-hydroxy-$\alpha$-tocopherone; Viola mandshurica; glutamate; antioxidant activity; protective effect; N18-RE-105 cells;
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