• Applied Biological Chemistry
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• v.41 no.1
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• pp.23-30
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• 1998

The cryIB, truncated cryIB$[cryIB({\alpha})]$, cryIA(b), and cytA genes, encoding 135-, 89-, 131-, and 27-kDa proteins, respectively, from Bacillus thuringiensis were cloned into a shuttle vector pBES and expressed in E. coli and Bacillus species. The morphology and solubility in alkaline buffer of the insecticidal crystal proteins were investigated. Transformation of intact cells of E. coli and Bacillus species was achieved by electroporation. High field strength of 11.0 kV/cm and resistance of 129 ohms were required for efficient transformation of E. coli strains and 4.5 kV/cm and 48 ohms for Bacillus species. Strains of recombinant E. coli and Bacillus species produced the insecticidal crystal proteins and accumulated as the same bipyramidal and irregular structures as those of CryIB and IA(b) and CytA of B. thuringiensls, respectively. The insecticidal crystal proteins accumulated in recombinant E. coli wire smaller in size than those in recombinant Bacillus species. The solubility in alkaline buffer of the insecticidal crystal proteins of recombinant E. coli increased gradually as the pH increased, whereas in the case of Bacillus species the solubility increased gradually as the pH increased up to 9 and then the solubility increased greatly up to two times higher than that of E. coli proteins.

• Korean journal of applied entomology
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• v.13 no.1 s.18
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• pp.25-31
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• 1974

This survey was aimed to accumulate basic data of aphid population at the Horticultural Experiment Station at Suweon. The yellow pan traps were setted at five locations (Fig.1.), and ran from May 1 to October 31. 1970. About one hundred and twenty species of aphids were trapped, including 24 species of plant vims vectors. Of these, dominant species were as follows: (Asterisk shows virus vector) Aphid species No. of catches * Aphis spiraecola PATCH 2,635, * Aphis craccivora KOCH 2,377, * Myzus persicae SULXER 2,111, Capitophorus hippophaes javanicus H.R. LAMBERS 2,051, Anoecia fulviabdominalis SASAKI 1,480, * Aphis gossypii GLOVER 867, * Macrosiphum avenae FABRICIUS 859, Cervaphis quercus TAKAHASHI 692, * Lipaphis erysimi KALTENBACH 645, Pleotrichophorus chrysanthemi THEOBALD 489, The above 10 species consisted $76.5\%$ of total catches and the 24 vector species consisted $55.5\%$. The curve of the seasonal occurrence of flying aphids at Horticultural Experiment Station shows bimodal, typical for the temperate region. The total number of trapped aphids at the Station from May to September, 1970, were less than that of average yearly catches at the College of Agriculture from 1967 to 1970. Thi, low numbers at Horticultural Experiment Station may attribute to the frequent spraying of insecticides from Spring to Summer on growing crops there. But the aphids population increase suddenly in the middle of October. This might be resulted from cease of insecticide applications and migration of aphids from summer host to winter host plants.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.6 no.11
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• pp.2784-2799
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• 2012

Biological data have been increased exponentially in recent years, and analyzing these data using data mining tools has become one of the major issues in the bioinformatics research community. This paper focuses on the protein construction process in higher organisms where the deoxyribonucleic acid, or DNA, sequence is filtered. In the process, "unmeaningful" DNA sub-sequences (called introns) are removed, and their meaningful counterparts (called exons) are retained. Accurate recognition of the boundaries between these two classes of sub-sequences, however, is known to be a difficult problem. Conventional approaches for recognizing these boundaries have sought for solely enhancing machine learning techniques, while inherent nature of the data themselves has been overlooked. In this paper we present an approach which makes use of the data attributes inherent to species in order to increase the accuracy of the boundary recognition. For experimentation, we have taken the data sets for four different species from the University of California Santa Cruz (UCSC) data repository, divided the data sets based on the species types, then trained a preprocessed version of the data sets on neural network(NN)-based and support vector machine(SVM)-based classifiers. As a result, we have observed that each species has its own specific features related to the splice sites, and that it implies there are related distances among species. To conclude, dividing the training data set based on species would increase the accuracy of predicting splicing junction and propose new insight to the biological research.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.35 no.3
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• pp.243-249
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• 1999

Specificity of catches has been analyzed to japanese tuna purse seine A principle component analysis was used to improve the efficiency of fishing and increase sustainable production and productivity of Korean tuna purse seine.The result are as follows;From the principal component analysis of the fish catches, the first principal component(Z1) to promote principal component score was skipjack Kastsuwonus Pelamis, LINNAEUS and yellowfin tuna Thunnus Albacares, BONNATERRE (Small : smaller than 10kg) and proportion was 86.8% of total. The second principal component(Z2) to increase principal component score was yellowfin tuna (Large : larger than 10kg) and proportion was 9.5%.On the other hand, fish operating that have caught skipjack and yellowfin tuna (Small and Larger) was not so much. Fish catches for one species raised volume of the catches while catches for multi-species decreased it since principal composition score for one species and both species together has been increased.Fish school could be divided into three groups of schools each of which was associated with drift objects, payaho and ship, school associated with shark, whale and porpoise and school of breezing, feeding and jumping from proportion of principal component analysis for fish catches of school types. However, the biological pattern is different among school associated with ship, payaho and school associated with drift objects for analysis eigen vector. School associated with ship, payaho and school associated with drifting object associated is judged as school which be assembled to vessel and drifted log temporary.

• Parasites, Hosts and Diseases
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• v.52 no.1
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• pp.63-67
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• 2014

This paper describes a new species of chigger mite (Acari: Trombiculidae), Gahrliepia cangshanensis n. sp., from rodents in southwest China. The specimens were collected from Yunnan red-backed voles, Eothenomys miletus (Thomas, 1914), and a Chinese white-bellied rat, Niviventer confucianus (Milne-Edwards, 1871) in Yunnan Province. The new species is unique mainly in its number of dorsal setae (n=21), and it has the following features: fT (formula of palpotarsus)=4B (B=branched), fp (formula of palpal seta)=B/N/N/N/B (N=naked), a broad tongue-shaped scutum with an almost straight posterior margin, and 17 PPLs (posterior posterolateral seta) with a length of 36-43 ${\mu}m$. This chigger mite may also infect other rodent hosts and may be distributed in other localities.

• Journal of Ecology and Environment
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• v.29 no.4
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• pp.353-360
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• 2006

Ecological correlates of flowering times often are examined to infer evolutionary mechanisms for flowering time diversities. We examined ecological characteristic associations such as growth habits and pollination modes with flowering times among 3,037 Korean angiosperms experiencing strong climatic seasonalities. We first examined taxonomic membership effects on flowering times across diverse taxonomic levels. Phylogeny constrained flowering times at all levels down to the genus level. We then analyzed the effects of ecological characteristics using subset data consisting of species randomly selected from each genus to control phylogenetic effects. The commonly observed patterns of early flowering of woody species in temperate regions existed. Spring flowering shrubs and trees, however, both being woody, were involved with biotic and abiotic vectors, respectively. In two herbaceous groups of annuals and perennials, annuals flowered later in the growing season than perennials although both herbs tended to be associated with abiotic vectors when flowering in autumn. These results support our hypothesis that species able to decouple vegetative and reproductive growth flower in spring's dry season, but species with different habits, even when they flower within the same season, are subjected to different selective pressures for efficient pollination.

• Bulletin of the Korean Chemical Society
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• v.20 no.12
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• pp.1413-1417
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• 1999

Chemical poisoning of Ni/MgO catalyst was induced by hot alkali carbonate vapor in molten carbonate fuel cell (MCFC), and the poisoned (or contaminated) catalyst was characterized by TPR/TPO, FTIR, and XRD analysis. Carbonate electrolytes such as K and Li were transferred to the catalyst during DIR-MCFC operation at 650 ℃. The deposition of alkali species on the catalyst consequently led to physical blocking on catalytic active sites and structural deformation by chemical poisoning. TPR/TPO analysis indicated that K species enhanced the reducibility of NiO thin film over Ni as co-catalyst, and Li species lessened the reducibility of metallic Ni by chemical reaction with MgO. FTIR analysis of the poisoned catalyst did not exhibit the characteristic ${\vector}_1$$(D_{3h})$ peaks (1055 $cm^{-1},\;1085\;cm{-1})$ for pure crystalline carbonates, instead a new peak (1120 $cm^{-1})$ was observed proportionally with deformed alkali carbonates. From XRD analysis, the oxidation of metallic Ni into $Ni_xMg_{1-x}O$ was confirmed by the peak shift of MgO with shrinking of Ni particles. Conclusively, hot alkali species induced both chemical poisoning and physical deposition on Ni/MgO catalyst in DIR-MCFC at 650 ℃.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.15 no.1
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• pp.36-40
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• 2010

Ballast water has been known as a major vector for global dispersal of toxic dinoflagellates and other microalgae. In this study, biodiversity in ships’ ballast water was examined using a dinoflagellate-oriented PCR primer set and species-specific real-time PCR. While motile dinoflagellates could be observe at very low cell densities by light microscopy,a wide range of dinoflagellate taxa including parasitic and phototrophic pico-dinoflagellates as well as harmful species to marine fish/shellfish was detected when techniques for cloning/sequencing of SSU rDNA of sample cells were used. Present result suggests that molecular methods including species-specific PCR primers may offer rapid and accurate detection of invasive species in ballast water.

• Mycobiology
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• v.41 no.1
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• pp.59-62
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• 2013

Japanese pine sawyer beetle (Monochamus alternatus) is an economically important pest in coniferous trees. Ophiostoma ips was isolated from the beetle and identified based on analysis of morphological properties and the ${\beta}$-tubulin gene sequence. The fungus easily produced perithecia with a long neck on malt extract agar and its ascospores were rectangular shaped. This is first report of Ophiostoma species associated with the pinewood nematode vector beetle in Korea.

• Current Research on Agriculture and Life Sciences
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• v.30 no.2
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• pp.124-130
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• 2012

Sweetpotato whitefly, Bemisia tabaci, is a vector of more than 100 plant-diseased viruses, as well as a serious pest of various horticultural plants. This species harbors a primary endosymbiont Portiera along with several secondary endosymbionts such as Cardinium and Hamiltonella. We investigated whether or not TYLCV acquisition alters the densities of endosymbionts in the body of B. tabaci using quantitative real-time PCR. Our results showed that the densities of both Cardinium and Hamiltonella, but not Portiera, increased upon acquisition of TYLCV. In addition, expression of GroEL, a molecular chaperone produced by Hamiltonella, was significantly upregulated in TYLCV-infected whiteflies. Our results suggest that endosymbionts may play an important role in TYLCV transmission mechanism within the body of B. tabaci.