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Molecular Detection of Harmful Dinoflagellates (Dinophyceae) in Ballast Water  

Park, Tae-Gyu (Aquaculture Environment Institute, National Fisheries Research and Development Institute (NFRDI))
Kim, Sung-Yeon (Aquaculture Environment Institute, National Fisheries Research and Development Institute (NFRDI))
Publication Information
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY / v.15, no.1, 2010 , pp. 36-40 More about this Journal
Abstract
Ballast water has been known as a major vector for global dispersal of toxic dinoflagellates and other microalgae. In this study, biodiversity in ships’ ballast water was examined using a dinoflagellate-oriented PCR primer set and species-specific real-time PCR. While motile dinoflagellates could be observe at very low cell densities by light microscopy,a wide range of dinoflagellate taxa including parasitic and phototrophic pico-dinoflagellates as well as harmful species to marine fish/shellfish was detected when techniques for cloning/sequencing of SSU rDNA of sample cells were used. Present result suggests that molecular methods including species-specific PCR primers may offer rapid and accurate detection of invasive species in ballast water.
Keywords
Ballast Water; Biodiversity; Dinoflagellate; Harmful Algae;
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1 Lin, S., Zhang, H., Hou, Y., Miranda, L. and Bhattacharya, D., 2006. Development of a dinoflagellate-oriented PCR primer set leads to detection of picoplanktonic dinoflagellates from Long Island Sound. Appl. Environ. Microbiol. 72: 5626-5630.   DOI   ScienceOn
2 Thompson, J.D., Gibson, T.J., Plewnaik, F., Jeanmougin, F. and Higgins, D.G., 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25: 4876-4882.   DOI
3 Bolch, C.J.S. and de Salas, M.F., 2007. A review of the molecular evidence for ballast water introduction of the toxic dinoflagellates Gymnodinium catenatum and the Alexandrium "tamarensiscomplex" to Australasia. Harmful Algae 6: 465-485.   DOI   ScienceOn
4 Botes, L., Smit, A.J. and Cook, P.A., 2002. The potential threat of algal blooms to the abalone (Haliotis midae) mariculture industry situated around the South African coast. Harmful Algae 2: 247-259.
5 Hallegraeff, G.M. and Bolch, C.J., 1992. Transport of diatom and dinoflagellate resting spores in ships' ballast water: implications for plankton biogeography and aquaculture. J. Plankton Res. 14: 1067-1084.   DOI
6 Posada, D. and Crandall, K.A., 1998. Modeltest: testing the model of DNA substation. Bioinfomatics 14: 817-818.   DOI   ScienceOn
7 Smayda, T.J., 2007. Reflections on the ballast water dispersal- harmful algal bloom paradigm. Harmful Algae 6: 601-22.   DOI   ScienceOn
8 Swofford, D.L., 1999. PAUP*. Phylogenetic analysis using parsimony (and other methods). Version 4.0. Sinauer Associates, Sunderland, MA.
9 Burkholder, J.M., Hallegraeff, G.M., Melia, G., Cohen, A., Bowers, H.A., Oldach, D.W., Matthew, W.P., Sullivan, M.J., Zimba, P.V., Aien, E.H., Kinder, C.A. and Mallin, M.A., 2007. Phytoplankton and bacterial assemblages in ballast water of U.S. military ships as a function of port of origin, voyage time, and ocean exchange practices. Harmful Algae 6: 486-518.   DOI   ScienceOn
10 Doblin, M.A., Popels, L.C., Coyne, K.J., Hutchins, D.A., Cary, S.C. and Dobbs, F.C., 2004. Transport of the harmful bloom alga Aureococcusano phagefferens by ocean going ships and coastal boasts. Appl. Environ. Microbiol. 70: 6495-6500.   DOI   ScienceOn
11 Huber, T., Faulkner G. and Hugenholtz P., 2004. Bellerophon: a program to detect chimeric sequences in multiple sequence alignments. Bioinformatics 20: 2317-2319.   DOI   ScienceOn
12 Place, A.R., Saito, K., Deeds, J.R., Robledo, J.A.F. and Vasta, G.R., 2008. A decade of research on Pfiesteria spp. and their toxins: unresolved questions and an alternative hypothesis. InBotana, L.M.(Eds) Seafood and freshwater toxins. CRC Press, New York, pp. 717-751.
13 Litaker, R.W., Vandersea, M.W., Kibler, S.R., Reece, K.S., Stokes, N.A., Steidinger, K.A., Millie, D.F., Bendis, B.J., Pigg, R.J. and Tester, P.A., 2003. Identification of Pfiesteria piscicida (Dinophyceae) and Pfiesteria-like organisms using internal transcribed spacer-specific PCR assays. J. Phycol. 39: 754-761.   DOI   ScienceOn
14 Lopez-Garcia, P., Rodriguez-Valera, F., Pedros-Allo, C. and Moreira, D., 2001. Unexpected diversity of small eukaryotes in deep-sea Antarctic plakton. Nature 409: 603-607.   DOI   ScienceOn
15 Park, T.G., Park, Y.T. and Lee, Y., 2009. Development of a SYTO9 based real-time PCR probe for detection and quantification of toxic dinoflagellate Karlodinium veneficum(Dinophyceae) in environmental samples Phycologia 48: 32-43.   DOI   ScienceOn