• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 1999년도 추계 학술대회논문집
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• pp.127-138
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• 1999

본 연구에서는 2 차원 캐비티 유동에서 경계조건 부여 방법의 차이에 따른 유체유동의 이력이 최종적인 정상상태에 미치는 효과를 수치실험을 통하여 관찰하였다. 그 결과 유동의 Reynolds 수가 작은 경우는 유동장이 가지는 이력이 시간의 경과와 함께 소멸하여 하나의 정상유동 상태를 가지는데 반하여, 큰 Reynolds 수에서는 유동장 이력이 소멸하지 않고 유지됨으로 인하여 다수의 정상유동 형태가 나타남이 확인이 되었다. 엄밀한 이론적 해석은 현재 이루워지지 않았으나 유체 운동량의 증가에 따라 Navier-Stokes 방정식의 비선형성이 영향을 미치고 있다고 여겨진다.

• 대기
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• 제23권3호
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• pp.245-264
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• 2013

Heavy rain over the Gangwon region has distinct characteristics in the temporal and spatial distribution of rainfall, most of which are concentrated on a very short period of time and either part of Yeongdong and Yeongseo regions. According to its regional distribution, heavy rain events over the Gangwon region may be classified into Yeongdong and Yeongseo heavy rain in which rainfalls of more than 110 mm $(6 hrs)^{-1}$ (heavy rain warning) have been observed in at least one of the weather stations over only Yeongdong or Yeongseo region, but over the other region the rainfalls are less than 70 mm $(6 hrs)^{-1}$ (heavy rain advisory). To differentiate between Yeongdong and Yeongseo heavy rain, 9 cases for Yeongdong heavy rain and 8 cases for Yeongseo heavy rain are examined on their synoptic and mesoscale environments using some meteorological parameters and ingredients. In addition, 8 cases are examined in which heavy rain warning or advisory are issued in both Yeongdong and Yeongseo regions. The cases for each heavy rain type have shown largely similar features in some meteorological parameters and ingredients. Based on an ingredient analysis, there are three common and basic ingredients for the three heavy rain types: instability, moisture, and lift. However, it is found that the distinct and important process producing strong upward vertical motions may discriminate among three heavy rain types very well. Yeongdong heavy rain is characterized by strong orographic lifting, Yeongseo heavy rain by high instability (high CAPE), and heavy rain over both regions by strong synoptic-scale ascent (strong 850 hPa Q-Vector convergence, diagnostics for ascent). These ingredients and diagnostics for the ingredients can be used to forecasting the potential for regional heavy rain. And also by knowing which of ingredients is important for each heavy rain type, forecasters can concentrate on only a few ingredients from numerous diagnostic and prognostic products for forecasting heavy rain events.

• Asian-Australasian Journal of Animal Sciences
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• 제23권6호
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• pp.806-813
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• 2010

Animal bioreactors have been regarded as alternative tools for the production of limited human therapeutic proteins. The mammary glands of cattle are optimal tissues to produce therapeutic proteins that cannot be produced in large amounts in traditional systems based on microorganisms and eukaryotic cells. In this study, two knock-in vectors, pBCTPOKI-6 and pBCTPOKI-10, which target the hTPO gene on the bovine beta-casein locus, were designed to develop cloned transgenic cattle. The pBCTPOKI-6 and pBCTPOKI-10 vectors expressed hTPO protein in culture medium at a concentration of 774 pg/ml and 1,867 pg/ml, respectively. Successfully, two targeted cell clones were obtained from the bovine fibroblasts transfected with the pBCTPOKI-6 vector. Cloned embryos reconstructed with the targeted nuclei showed a lower in vitro developmental competence than those with the wild-type nuclei. After transfer of the cloned embryos into recipients, 7 pregnancies were detected at 40 to 60 days of gestation, but failed to develop to term. The results are the first trial for targeting of a human gene on the bovine milk protein gene locus, providing the potential for a large-scale production of therapeutic proteins in the animal bioreactor system.

• Asian Pacific Journal of Cancer Prevention
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• 제15권14호
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• pp.5917-5920
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• 2014

Background: This study aimed to explore the effects of ras association domain family 1 A (RASSF1A) on proliferation and apoptosis of human cervical cancer cell line Hela cells. Materials and Methods: RASSF1A was cloned into the pcDNA3.1(+) vector to generate pcDNA3.1(+)-RASSF1A plasmid for transfection into Hela cells. Changes in the proliferation and apoptosis of cultured Hela cells were examined by the 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium chloride assay and flow cytometry. A protein array was used to analyze the expression of apoptotic factors. Results: Plasmid pcDNA3.1(+)-RASSF1A was generated and transfected into Hela cells to stably express RASSF1A in Hela cells. RASSF1A transfection was effective in inhibiting the proliferation of Hela cells up to 52.4%, as compared to cells transfected with an empty plasmid. RASSF1A expression also successfully induced apoptosis in human cervical cells with an apoptosis rate of 20.5%. More importantly, protein array results showed that RASSF1 A transfection induced overexpression of p21 and caspase 8, while decreasing the expression of survivin in Hela cells. Conclusions: RASSF1A expression was effective in suppressing the proliferation and increasing apoptosis of Hela cells, and may be a potential therapy for cervical cancer in clinic.

• 한국정보통신학회논문지
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• 제19권10호
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• pp.2457-2464
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• 2015

무선 네트워크를 통한 네트워크 제어 시스템 (NCS: Networked Control Systems)은 다양한 제어 시스템의 효율성을 극대화 할 수 있는 잠재력을 가지고 있다. 본 연구에서는 네트워크 제어 시스템으로부터 이끌어낸 상태갱신주기(SUI: State Update Interval)라는 성능 메트릭을 정의한다. 제시된 상태갱신주기는 제어와 통신 시스템 계층 사이의 핵심적인 상호작용을 포함하고 있다. 제시된 성능 메트릭은 NCS를 위한 최적화 문제를 도출하는데 사용이 되었으며, 이러한 최적화 문제의 목적 함수는 상태갱신주기의 제한 요소를 만족하는 확률이며 변수는 매체접근 확률로 표현되었다. 본 연구에서는 제시된 최적화 문제의 최적 매체접근 확률이 유일한 해를 가진다는 것을 증명하였다. 또한 NCS를 위한 최적의 매체접근 확률은 전송률을 극대화하는 기존의 매체접근 확률보다 낮다는 결론을 도출하였다. 성능 분석 결과를 통하여 시스템의 안정도가 상태갱신주기를 고려한 최적의 매체접근 확률을 사용하였을 때가 전송률을 최대화하는 매체접근 확률을 사용 했을 때 보다 높다는 점을 보였다.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 1998년도 춘계 학술대회논문집
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• pp.15-28
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• 1998

As an alternative for solving the incompressible Navier-Stokes equations, we present a vorticity-based integro-differential formulation for vorticity, velocity and pressure variables. One of the most difficult problems encountered in the vorticity-based methods is the introduction of the proper value-value of vorticity or vorticity flux at the solid surface. A practical computational technique toward solving this problem is presented in connection with the coupling between the vorticity and the pressure boundary conditions. Numerical schemes based on an iterative procedure are employed to solve the governing equations with the boundary conditions for the three variables. A finite volume method is implemented to integrate the vorticity transport equation with the dynamic vorticity boundary condition . The velocity field is obtained by using the Biot-Savart integral derived from the mathematical vector identity. Green's scalar identity is used to solve the total pressure in an integral approach similar to the surface panel methods which have been well-established for potential flow analysis. The calculated results with the present mettled for two test problems are compared with data from the literature in order for its validation. The first test problem is one for the two-dimensional square cavity flow driven by shear on the top lid. Two cases are considered here: (i) one driven both by the specified non-uniform shear on the top lid and by the specified body forces acting through the cavity region, for which we find the exact solution, and (ii) one of the classical type (i.e., driven only by uniform shear). Secondly, the present mettled is applied to deal with the early development of the flow around an impulsively started circular cylinder.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2002년도 생물공학의 동향 (X)
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• pp.534-537
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• 2002

본 연구에서는 기능성 식품 첨가물 소재 또는 의약품으로 사용 가능한 한천올리고당의 대량생산을 위하여 agarase 생산균주인 Bacillus cereus ASK202에 대해 유전자 cloning 방법을 이용하여 한천분해효소 고생산성 균주로의 개발을 시도하였다. 원균주의 chromosomal DNA를 무작위적으로 절단하여 agarase를 생산하는 gene 부위를 선별한 결과, 83,300 Da의 agarase(Eba1)를 생산하는 재조합 균주 E. coli BL21(DE3)/pEBA1를 얻을 수 있었다. E. coli BL21(DE3)/pEBAl에서 유도물질로 IPTG를 첨가한 후 induction 5시간 후에 agarase가 원균주에 비해 8배 증가한 양이 고발현되었다. 발현된 agarase는 Asx(Aspartic acid, Asparagine), Glycine와 같은 산성 아미노산의 함량과 Alanine과 같은 중성 아미노산의 함량이 높았으며, pH 5.6, $40^{\circ}C$에서 최적의 활성을 나타내었다. 최적 기질 agar에 대한 $K_m$과 $V_{max}$값은 0.068 mg/$m{\ell}$과 0.094mg/$m{\ell}{\cdot}min$으로 한천의 ${\beta}$-결합을 자르는 ${\beta}$-agarase로 판명되었다.

• 한국정보과학회논문지:소프트웨어및응용
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• 제28권11호
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• pp.864-873
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• 2001

본 논문에서는 공개키 기반 등록기관의 사용자 신원 확인 기능의 안정성과 신뢰성 향상을 위한 방법으로 홍채인식 기술을 활용한 등록기관 시스템을 제시한다. 제안하는 시스템은 홍채 패턴의 처리 기능에 따라 홍채영상 획득, 홍채정보 등록, 홍채정보 검증의 세 개의 모듈로 구성되며, 각 모듈간의 등록 정보의 교환은 대칭키 암호 알고리즘으로 암호화를 해서 안전하게 송수신이 이루어진다. 홍채 패턴의 효율적 표상과 비교를 위한 홍채 특징 정보의 추출방법으로서는 웨이브렛 변환을 이용한 영상의 다중분할 과정을 통해 적은 차원의 정보를 추출하여 효과적인 특징벡터를 구성하는 방법을 사용한다. 적용된 홍채인식 방법에 대한 실험을 통해서, 생체인식 기술의 새로운 분야로의 적용 가능성을 살펴본다.

• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.160-166
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• 2008

A DNA fragment containing 2,079 base pairs from Bacillus circulans CGMCC 1416 was cloned using degenerate PCR and inverse PCR. An open reading frame containing 981 bp was identified that encoding 326 amino acids residues, including a putative signal peptide of 31 residues. The deduced amino acid sequence showed the highest identity (68.1%) with $endo-{\beta}-1,4-D-mannanase$ from Bacillus circulans strain K-1 of the glycoside hydrolase family 5 (GH5). The sequence encoding the mature protein was cloned into the pET-22b(+) vector and expressed in Escherichia coli as a recombinant fusion protein containing an N-terminal hexahistidine sequence. The fusion protein was purified by $Ni^{2+}$ affinity chromatography and its hexahistidine tag cleaved to yield a 31-kDa ${\beta}$-mannanase having a specific activity of 481.55U/mg. The optimal activity of the purified protein, MANB48, was at $58^{\circ}C$ and pH 7.6. The hydrolysis product on substrate locust bean gum included a monosaccharide and mainly oligosaccharides. The recombinant MANB48 may be of potential use in the feed industry.

• Journal of Microbiology and Biotechnology
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• 제20권11호
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• pp.1491-1499
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• 2010

Rhizosphere microorganisms possessing phytase activity are considered important for rendering phytate-phosphorus (P) available to plants. In the present study, the Citrobacter braakii phytase gene (appA) was overexpressed in rhizobacteria possessing plant growth promoting (PGP) traits, for increasing their potential as bioinoculants. AppA was cloned under the lac promoter in the broadhost-range expression vector pBBR1MCS-2. Transformation of the recombinant construct pCBappA resulted in high constitutive phytase activity in all of the eight rhizobacterial strains belonging to genera Pantoea, Citrobacter, Enterobacter, Pseudomonas (two strains), Rhizobium (two strains), and Ensifer that were studied. Transgenic rhizobacterial strains were found to display varying levels of phytase activity, ranging from 10-folds to 538-folds higher than the corresponding control strains. The transgenic derivative of Pseudomonas fluorescens CHA0, a well-characterized plant growth promoting rhizobacterium, showed the highest expression of phytase (~8 U/mg) activity in crude extracts. Although all transformants showed high phytase activity, rhizobacteria having the ability to secrete organic acid showed significantly higher release of P from Ca-phytate in buffered minimal media. AppA overexpressing rhizobacteria showed increased P content, and dry weight (shoot) or shoot/ root ratio of mung bean (Vigna radiata) plants, to different extents, when grown in semisolid agar (SSA) medium containing Na-phytate or Ca-phytate as the P sources. This is the first report of the overexpression of phytase in rhizobacterial strains and its exploitation for plant growth enhancement.