• Journal of Microbiology and Biotechnology
• /
• 제27권5호
• /
• pp.965-974
• /
• 2017

The single-chain variable fragment (scFv) against lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is a promising molecule for its potential use in the diagnosis and immunotherapy of atherosclerosis. Producing this scFv in several milligram amounts could be the starting point for further engineering and application of the scFv. In this study, the abundant expression of the anti-LOX-1 scFv was attempted using Escherichia coli (E. coli) and Brevibacillus choshinensis (B. choshinensis). The scFv had limited soluble yield in E. coli, but it was efficiently secreted by B. choshinensis. The optimized fermentation was determined using the Plackett-Burman screening design and response surface methodology, under which the yield reached up to 1.5 g/l in a 5-L fermentor. Moreover, the properties of the scFvs obtained from the two expression systems were different. The antigen affinity, transition temperature, and particle diameter size were 1.01E-07 M, $55.2{\pm}0.3^{\circ}C$, and 9.388 nm for the scFv expressed by B. choshinensis, and 4.53E-07 M, $52.5{\pm}0.3^{\circ}C$, and 13.54 nm for the scFv expressed by E. coli. This study established an efficient scale-up production methodology for the anti-LOX-1 scFv, which will boost its use in LOX-1-based therapy.

• 대한기계학회논문집B
• /
• 제21권2호
• /
• pp.285-293
• /
• 1997

Numerical calculations has been performed for the flow and heat transfer to a circular cylinder from a hot circular impinging air jet. The characteristics of the flow and heat transfer are investigated and compared with the two-dimensional flow. The present study lays emphasis on the investigation on the flow and heat transfer of the three-dimensionality. The effects of the buoyancy force and the size of jet are also studied. The noticeable difference between the three and the two-dimensional cases is that there is axial flow of low temperature into the center-plane of the cylinder from the outside in the recirculation region. Local Nusselt number over the cylinder surface has higher value for the large jet as compared with that of the small jet since the energy loss of hot jet to the ambient air decreases with increase of the jet size. As buoyancy force increases the flow accelerates so that the period of cooling by the ambient air is reduced, which results in higher local Nusselt number over the surface.

• 천문학회지
• /
• 제26권2호
• /
• pp.115-134
• /
• 1993

The light curve and radial velocity curve of multiperiodic dwarf cepheid VI719 Cyg (HD200925) with peculiar light curve have been reanalyzed in order to identify the oscillation modes to confirm the helium settling within the envelope. To do these, through the period search for the photometric and radial velocity data from the literature, two different periods were determined and the oscillation modes corresponding to the first and second periods were identified as the fundamental and first radial overtones. Hence the helium settling within the envelope was confirmed from the period ratio. The color excess, metallicity, effective temperature, and surface gravity corresponding to two different modes were determined and it was found that these parameters almost do not depend upon different oscillation mode. By utilizing the surface brightness method, we investigated the variation of angular diameter and radial displacement and it was found that the angular variation is very peculiar. Also by referring to the stellar models, mass and age were determined as $2.7M_{\bigodot}$ and 0.42 Gyr respectively which make this variable star heavier and younger than other multimode dwarf cepheids. Preliminary spectroscopic CCD observations were carried out and it was found that Mg in VI719 Cygni is nearly solar abundent according to the analysis of $5172.68{\AA}MgI$ line which is inconsistent with the photometric result. It was suggested that VI719 Cyg may be classified as a $\rho$ Pup stars according to the photometric characteristics.

• 전력전자학회:학술대회논문집
• /
• 전력전자학회 1998년도 Proceedings ICPE 98 1998 International Conference on Power Electronics
• /
• pp.774-779
• /
• 1998

For the speed control of motors, the position or speed sensors are necessary to obtain the position information of the rotor. Specially, SRM(Switched Reluctance Motor) needs an accurate rotor position data because both the rotor and the stator have a salient pole structure. High functional sensors like resolver or encoder are expensive and have complex connecting lines to the controller so the pure signals are apt to be mixed with noised. In the sight of SRM drives, the high temperature, heavy dust, and the EMI surroundings reduce the reliability of speed and position sensors. Therefore, the speed and position sensorless control algorithms using observer have been accepted widely. In this paper An adaptive sliding observer is described to control the SRM without speed or position sensors. The adaptive sliding observer is set on the basis of variable structure control theory. The sliding surface is constructed by current error terms and this surface guarantees the errors converge to "zero". The stability of observer is affirmed by Lyapunov stability analysis and popov's hyper stability theory.ty theory.

• 한국정밀공학회지
• /
• 제27권12호
• /
• pp.28-32
• /
• 2010

Technical demands for aspheric glass lens formed in market increases its application from simple camera lens module to fiber optics connection module in optical engineering. WC is often used as a metal core of the aspheric glass lens, but the long life time is issued because it fabricated in high temperature and high pressure environment. High hard thin film coating of lens core increases the core life time critically. Diamond Like Carbon(DLC) thin film coating shows very high hardness and low surface roughness, i.e. low friction between a glass lens and a metal core, and thus draw interests from an optical manufacturing industry. In addition, DLC thin film coating can removed by etching process and deposit the film again, which makes the core renewable. In this study, DLC films were deposited on the SiC ceramic core. The process variable in FVA(Filtered Vacuum Arc) method was the substrate bias-voltage. Deposited thin film was evaluated by raman spectroscopy, AFM and nano indenter and measured its crystal structure, surface roughness, and hardness. After applying optimum thin film condition, the life time and crystal structure transition of DLC thin film was monitored.

• 한국세라믹학회지
• /
• 제37권4호
• /
• pp.345-353
• /
• 2000

Carbon nanofibers were prepared from the decomposition of various carbon-containing gases over pure Ni, pure Fe and their alloys with Cu. They yields, properties, and structure of carbon nanofibers obtained from the various reaction conditions were analyzed. Type of reacting gas, reaction temperature and catalyst composition were changed as the reaction variable. With Ni-Cu catalysts, the maximum yields of carbon nanofibers were obtained at temperatures between 550 and 650$^{\circ}C$ according to the reacting gas mixtures of C2H2-H2, C2H4-H2 and C3H8-H2, and the surface areas of the carbon nanofibers produced were 20∼350㎡/g. In the case of CO-H2 mixture, the rapid deposition of carbon nanofibers occurred with Fe-Cu catalyst and the maximum yield were obtained around 550$^{\circ}C$ with the range of surface areas of 140∼170㎡/g. The electrical resistivity of carbon nanofiber regarded as the key property of filler for the application of electromagnetic interference shielding was very sensitive to the type of reactant gas and the catalyst composition ranging 0.07∼1.5Ωcm at a pressure of 10000 psi, and the resistivity of carbon nanofibers produced over pure nickel catalyst were lower than those over alloy catalysts. SEM observation showed that the carbon nanofibers produced had the diameters ranging 20∼300 nm and the straight structure of carbon nanofibers changed into the twisted or helical conformation by the variation of reacting gas and catalyst composition.

• 한국표면공학회지
• /
• 제47권6호
• /
• pp.354-361
• /
• 2014

In order to utilize silicon carbide (SiC) as an inner part of fluidized bed reactor (FBR) for manufacturing poly-silicon, we have carried out the thermodynamic calculation on the overall reactions including poly-silicon synthesis and compatibility of SiC with FBR process. The resources of silicon included $SiH_4(MS)$, $SiHCl_3(TCS)$ and $SiCl_4(STC)$ and the thermodynamic yield of the FBR with MS, TCS and STC were compared each other with variable range of temperature, pressure and hydrogen to silicon ratio. The silicon yield of MS, TCS and STC were 100%, 28% and 4%, respectively, throughout the conventional FBR conditions. Silicon carbide having high hardness and strength showed strong resistance to granule collisions during the FBR process using a lab-scale reactor. And it also showed quite good compatibility with the typical FBR processes of MS and TCS resources.

• 한국산학기술학회논문지
• /
• 제16권12호
• /
• pp.8306-8313
• /
• 2015

개방형 전류측정기는 DC 모터 콘트롤러, AC 가변 콘트롤러, UPS(Uninterruptible Power System)에 주로 사용되며 최근 신재생 에너지의 성장과 전력망의 스마트 그리드화로 인하여 활용성이 점차 확대되고 있다. 이러한 신재생 에너지의 성장으로 관련 핵심기술의 보유여부가 중요해지고 있으며 대부분 수입에 의존하고 있는 개방형 전류측정기의 국산화 및 관련 기술의 확보를 필요로 한다. 본 논문에서는 일반 산업용 개방형 전류측정기의 제작과정과 특성을 측정한 결과를 기술하였다. 개방형 전류측정기를 구성하는 C형 철심의 공극자장분포 해석 및 형상 설계, 홀센서의 선정 및 특성시험, 정전류 전원공급회로와 신호처리회로의 회로설계 과정을 기술하며 DIP(Dual In-line Package) type과 SMD (Surface Mount Device) type의 100A급 개방형 전류측정기를 제작하고 특성을 측정했다. 제작된 전류측정기로 0~100A 범위에서 통전 실험을 실시한 했고 직류전류와 60Hz의 교류전류에서 특성을 측정한 결과 정밀도 오차 2% 이내, 선형도 오차 2% 이내의 성능을 만족하였다. 또한 부 특성 온도계수를 갖는 NTC(Negative Temperature Coefficient) 서미스터를 이용한 온도보상회로를 사용하여 $-35{\sim}100^{\circ}C$의 범위에서 온도보상 효과를 확인하였다.

• 한국농림기상학회지
• /
• 제9권3호
• /
• pp.195-202
• /
• 2007

수평면과 경사면간의 일사수광량 편차의 정규화변량인 과열지수는 산악지형에서 일 최고기온 추정오차를 줄이는데 효과가 있다고 알려져 있다. 하지만 계산의 복잡성 때문에 경사도와 경사방향에 근거한 매개변량인 개방지수를 먼저 계산하고 이를 이용하여 과열지수를 간접적으로 추정하는 방법이 널리 쓰이고 있다. 이러한 개방지수 매개 간접추정법이 우리나라와 같이 복잡한 산악지형에서 일 최고기온 추정에 적합한지를 알아보기 위해 강원도 평창군에 위치한 도암댐 표준유역(면적 $149 km^2$, 표고범위 $651{\sim}1,445m$)을 대상으로 야외실험을 실시하였다. 이 지역의 30m 해상도 수치고도모형으로부터 시험유역의 166,050개 격자점의 개방지수와 과열지수를 계산하였다. 이들 간의 관계를 계절별 최적 회귀모형으로 나타내고 이로부터 매월 15일의 과열지수를 개방지수로부터 추정하였다. 인근 대관령기상대와 비슷한 고도(해발 844m)에 경사방향이 서로 다른 7개 지점을 선정하여 온도계를 설치하고 2006년 6월부터 2007년 2월까지의 기온을 10분 간격으로 측정하였다. 추정오차를 종속변수로, 각 지점의 추정 과열지수를 설명변수로 둔 회귀분석을 실시한 결과 결정계수가 겨울 0.46, 가을 0.24, 여름 0.02로서 직접 계산된 과열지수를 설명변수로 하는 경우(겨울 0.52, 가을 0.37, 여름 0.15)에 비해 신뢰도가 떨어진다는 것을 확인하였다.

• 한국식품영양과학회지
• /
• 제35권8호
• /
• pp.1051-1056
• /
• 2006

온도와 ATP 증가에 따라 사과.당근 배합비율과 상관없이 혼합주스의 투과플럭스가 비례적으로 증가했으며, 특히 온도변화에 따른 투과플럭스의 증가가 뚜렷했다. 사과와 당근비가 3:1인 실험구에서 가용성 고형분과 총당의 함량이 높게 측정되었고, 가용성 고형분의 경우 시료배합비율의 영향이, 총당의 경우 온도의 영향이 큰 것으로 나타났다. 시료배합비율에 따른 비타민 C 함량차이는 크지 않았으나 당근의 함량이 많을수록 비타민 C의 함량이 높게 측정되었고, 압력증가에 따른 비타민 C 함량 증가가 뚜렷했다. 사과의 함량이 높을수록 산도도 높았고, 온도증가에 따른 산도의 증가도 뚜렷했다. 한외여과 결과, 전 시료에 걸쳐 우수한 청징효과를 나타내었고, 사과와 당근비가 3:1인 실험구에서 그 청징효과가 두드러졌으며, 또한 점도변화도 가장 컸다. 또한 공정최적화 결과 공정온도 및 ATP가 각각 $44.97^{\circ}C$, 113.57 kPa이고 사과의 혼합비율이 28.50%인 경우 $flux=0.216\;L/m^2.h$, soluble $solids=10.39^{\circ}Brix$, total sugar=71.32 mg/mL, vitamin C=315.18 mg%, acidity=7.78 mL, turbidity=0.017, viscosity=1.44 cp인 사과.당근 혼합과채주스를 제조할 수 있는 것으로 나타났다.