• Pediatric Infection and Vaccine
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• v.25 no.2
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• pp.101-106
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• 2018

A 6-year-old boy with underlying hemolytic anemia of unknown etiology, atopic dermatitis, and recurrent urticaria visited our hospital because of acute respiratory failure induced by influenza A. Despite mechanical ventilation after endotracheal intubation along with inhalation of nitric oxide, respiratory acidosis and hypoxemia persisted. Veno-venous extracorporeal membrane oxygenation (VV ECMO) insertion was performed to provide respiratory support. After performing flexible bronchoscopy, we found that thick mucus plugs were obstructing the right bronchus intermedius and the upper lobe orifice. After bronchial washing and removal of the plugs, we were able to wean the patient off VV ECMO and transfer him to the general ward. He was discharged without any neurologic or pulmonary sequelae.

• Korean Journal of Remote Sensing
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• v.36 no.4
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• pp.535-544
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• 2020

In synthetic aperture radar (SAR) based ship detection application, false alarms frequently occur due to various noises caused by the radar imaging process. Among them, radio frequency interference (RFI) and azimuth smearing produce substantial false alarms; the latter also yields longer length estimation of ships than the true length. These two noises are prominent at cross-polarization and relatively weak at co-polarization. However, in general, the cross-polarization data are suitable for ship detection, because the radar backscatter from background sea surface is much less in comparison with the co-polarization backscatter, i.e., higher ship-sea image contrast. In order to improve the ship detection accuracy further, the RFI and azimuth smearing need to be mitigated. In the present letter, Sentinel-1 VV- and VH-polarization intensity data are used to show a novel technique of removing these noises. In this method, median image intensities of noises and background sea surface are calculated to yield arithmetic tendency. A band-math formula is then designed to replace the intensities of noise pixels in VH-polarization with adjusted VV-polarization intensity pixels that are less affected by the noises. To verify the proposed method, the adaptive threshold method (ATM) with a sliding window was used for ship detection, and the results showed that the 74.39% of RFI false alarms are removed and 92.27% false alarms of azimuth smearing are removed.

• Journal of Microbiology
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• v.43 no.6
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• pp.537-545
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• 2005

Even though neutralizing antibodies against the Hantaan virus (HTNV) has been proven to be critical against viral infections, the cellular immune responses to HTNV are also assumed to be important for viral clearance. In this report, we have examined the cellular and humoral immune responses against the HTNV nucleocapsid protein (NP) elicited by virus infection or DNA vaccination. To examine the cellular immune response against HTNV NP, we used $H-2K^b$ restricted T-cell epitopes of NP. The NP-specific $CD8^+$ T cell response was analyzed using a $^{51}Cr-release$ assay, intracellular cytokine staining assay, enzyme-linked immunospot assay and tetramer binding assay in C57BL/6 mice infected with HTNV. Using these methods, we found that HTNV infection elicited a strong NP-specific $CD8^+$ T cell response at eight days after infection. We also found that several different methods to check the NP-specific $CD8^+$ T cell response showed a very high correlation among analysis. In the case of DNA vaccination by plasmid encoding nucleocapsid gene, the NP-specific antibody response was elicited $2\~4$ weeks after immunization and maximized at $6\~8$ weeks. NP-specific $CD8^+$ T cell response reached its peak 3 weeks after immunization. In a challenge test with the recombinant vaccinia virus expressing NP (rVV-HTNV-N), the rVV-HTNV-N titers in DNA vaccinated mice were decreased about 100-fold compared to the negative control mice.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.2
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• pp.155-161
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• 2005

The inherent instability of metabolite production in plant cell culture-based bioprocessing is a major problem hindering its commercialization. To understand the extent and causes of this instability, this study was aimed at understanding the variability of anthocyanin accumulation during long-term subcultures, as well as within subculture batches, in Vitis vinifera cell cultures. Therefore, four cell line suspensions of Vitis vinifera L. var. Gamay Freaux, A, B, C and D, originated from the same callus by cell-aggregate cloning, were established with starting anthocyanin contents of $2.73\;\pm\;0.15,\;1.45\;\pm\;0.04,\;0.7\;\pm\;0.024\;and\;0.27\;\pm\;0.04$CV (Color Value)/g-FCW (fresh cell weight), respectively. During weekly subculturing of 33 batches over 8 months, the anthocyanin biosynthetic capacity was gradually lost at various rates, for all four cell lines, regardless of the significant difference in the starting anthocyanin content. Contrary to this general trend, a significant fluctuation in the anthocyanin content was observed, but with an irregular cyclic pattern. The variabilities in the anthocyanin content between the subcultures for the 33 batches, as represented by the variation coefficient (VC), were 58, 57, 54, and $84\%$ for V. vinifera cell lines A, B, C and D, respectively. Within one subculture, the VCs from 12 replicate flasks for each of 12 independent subcultures were averaged, and found to be $9.7\%$, ranging from 4 to $17\%$. High- and low-producing cell lines, VV05 and VV06, with 1.8-fold differences in their basal anthocyanin contents, exhibited different inducibilities to L-phenylalanine feeding, methyl jasmonate and light irradiation. The low-producing cell line showed greater potential in enhanced the anthocyanin production.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.2
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• pp.85-91
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• 2014

Ground-based polarimetric scatterometers have been effective tools to monitor the growth of crop with multi-polarization and frequencies and various incident angles. An important advantage of these systems that can be exploited is temporal observation of a specific crop target. Polarimetric backscatter data at L-, C- and X-bands were acquired every 10 minutes. We analyzed the relationships between L-, C- and X-band signatures, biophysical measurements over the whole corn growth period. The Vertical transmit and Vertical receive polarization (VV) backscattering coefficients for all bands were greater than those of the Horizontal transmit and Horizontal receive polarization (HH) until early-July, and then thereafter HH-polarization was greater than VV-polarization or Horizontal transmit and Vertical receive polarization (HV) until the harvesting stage (Day Of Year, DOY 240). The results of correlation analysis between the backscattering coefficients for all bands and corn growth data showed that L-band HH-polarization (L-HH) was the most suited for monitoring the fresh weight ($r=0.95^{***}$), dry weight ($r=0.95^{***}$), leaf area index ($r=0.86^{**}$), and vegetation water content ($r=0.93^{***}$). Retrieval equations were developed for estimating corn growth parameters using L-HH. The results indicated that L-HH could be used for estimating the vegetation biophysical parameters considered here with high accuracy. Those results can be useful in determining frequency and polarization of satellite Synthetic Aperture Radar stem and in designing a future ground-based microwave system for a long-term monitoring of corn.

• Journal of the Korea Convergence Society
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• v.8 no.1
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• pp.89-96
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• 2017

This study is to analyze seven fluoride varnishes remineralizing effects on early carious lesions in the clinical field. While CS, MI, CW, VV, EP, FP and FN were used for permanent teeth, CS, MI, CW and FP were used for primary teeth. The specimens were demineralized for 4 days after forming a $3{\times}3mm^2$ window with nail varnish, applied with varnishes separately, then were stirred in artificial saliva for 1 week. QLF-D analysis of carious lesion size showed that permanent teeth's ${\Delta}F$ value decreased after applying CS(4,64), MI(4.86), CW(4.50), and EP(5.50), while for primary teeth, FP(3.00) and MI(3.00) displayed a remineralizing effect. Thus, MI can be used effectively for preventing caries for both adults and children in the clinical field.

• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1197-1206
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• 2005

Vibrio vulnificus is an opportunistic pathogen that causes a septicemia and expresses numerous virulence factors, in which luxS and smcR are genes encoding for components responsible for quorum-sensing regulation. In the present study, null mutants were constructed with lesions in each or both of these two genes from the V. vulnificus Vv$\Delta$Z strain, which is a lacZ$^{-}$ and chloramphenicol/streptomycin-resistant derivative of the wild-type ATCC29307 strain, and their phenotypes related to virulence were compared with those of the parental cells. $LD_{50}$ and histopathological findings of luxS-, smcR-, or luxS- smcR- deficient mutant were not different from those of the parent strain, a lacZ-deficient streptomycin-resistant strain in mice. However, time of death in mice was delayed, and numbers of bacteria survived in bloodstream after intraperitoneal injection in mice were decreased by mutation, especially luxS and smcR double mutant (VvSR$\Delta$ZSR). These phenomena were supported by increased serum sensitivity and delayed bacterial proliferation in both murine blood and iron-restricted medium. These results suggest that the luxS and luxR homologous genes in V. vulnificus could playa role in bacterial survival in host by enhancing proliferation and adjusting to changed environment.

• Korean Journal of Remote Sensing
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• v.38 no.1
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• pp.111-126
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• 2022

Land use and land cover (LULC) mapping is an important factor in geospatial analysis. Although highly precise ground-based LULC monitoring is possible, it is time consuming and costly. Conversely, because the synthetic aperture radar (SAR) sensor is an all-weather sensor with high resolution, it could replace field-based LULC monitoring systems with low cost and less time requirement. Thus, LULC is one of the major areas in SAR applications. We developed a LULC model using only KOMPSAT-5 single co-polarized data and digital elevation model (DEM) data. Twelve HH-polarized images and 18 VV-polarized images were collected, and two HH-polarized images and four VV-polarized images were selected for the model testing. To train the LULC model, we applied the conditional generative adversarial network (cGAN) method. We used U-Net combined with the residual unit (ResUNet) model to generate the cGAN method. When analyzing the training history at 1732 epochs, the ResUNet model showed a maximum overall accuracy (OA) of 93.89 and a Kappa coefficient of 0.91. The model exhibited high performance in the test datasets with an OA greater than 90. The model accurately distinguished water body areas and showed lower accuracy in wetlands than in the other LULC types. The effect of the DEM on the accuracy of LULC was analyzed. When assessing the accuracy with respect to the incidence angle, owing to the radar shadow caused by the side-looking system of the SAR sensor, the OA tended to decrease as the incidence angle increased. This study is the first to use only KOMPSAT-5 single co-polarized data and deep learning methods to demonstrate the possibility of high-performance LULC monitoring. This study contributes to Earth surface monitoring and the development of deep learning approaches using the KOMPSAT-5 data.

• Proceedings of the KSRS Conference
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• 1999.11a
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• pp.485-490
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• 1999

Wind vector information over the ocean is currently obtained using multiple beam scatterometer data. The scatterometers on ERS-1/2 generate wind vector information with a spatial resolution of 50km and accuracies of $\pm$2m/s in wind speed and $\pm$20$^{\circ}$ in wind direction. Synthetic aperture radar (SAR) data over the ocean have the potential of providing wind vector information independent of weather conditions with finer resolution. Finer resolution wind vector information can often be useful particularly in coastal regions where the scatterometer wind information is often corrupted because of the lower resolution system characteristics which is often contaminated by the signal returns from the coastal areas or ice in the case of arctic environments. In this paper we tested CMOD_4 and CMOD_IFR2 algorithms for extracting the wind vector from SAR data. These algorithms require precise estimation of normalized radar cross-section and wind direction from the SAR data and the local incidence angle. The CMOD series algorithms were developed for the C-band, VV-Polarized SAR data, typically for the ERS SAR data. Since RADARSAT operates at the same C-band but with HH-Polarization, the CMOD series algorithms should not be used directly. As a preliminary approach of resolving with this problem, we applied the polarization ratio between the HH and VV polarizations in the wind vectors estimation. Two test areas, one in front of Inchon and several sites around Jeju island were selected and investigated for wind vector estimation. The new results were compared with the wind vectors obtained from CMOD algorithms. The wind vector results agree well with the observed wind speed data. However the estimation of wind direction agree with the observed wind direction only when the wind speed is greater than approximately 3.0m/s.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.127-134
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• 2012

Microwave remote sensing can help monitor the land surface water cycle, crop growth and soil moisture. A ground-based polarimetric scatterometer has an advantage for continuous crop using multi-polarization and multi-frequencies and various incident angles have been used extensively in a frequency range expanding from L-band to Ka-band. In this study, we analyzed the relationships between L-, C- and X-band signatures and soil moisture content over the whole soybean growth period. Polarimetric backscatter data at L-, C- and X-bands were acquired every 10 minutes. L-band backscattering coefficients were higher than those observed using C- or X-band over the period. Backscattering coefficients for all frequencies and polarizations increased until Day Of Year (DOY) 271 and then decreased until harvesting stage (DOY 294). Time serious of soil moisture content was not a corresponding with backscattering over the whole growth stage, although it increased relatively until early August (R2, DOY 224). We conducted the relationship between the backscattering coefficients of each band and soil moisture content. Backscattering coefficients for all frequencies were not correlated with soil moisture content when considered over the entire stage ($r{\leq}0.50$). However, we found that L-band HH polarization was correlated with soil moisture content (r=0.90) when Leaf Area Index (LAI)<2. Retrieval equations were developed for estimating soil moisture content using L-band HH polarization. Relation between L-HH and soil moisture shows exponential pattern and highly related with soil moisture content ($R^2=0.92$). Results from this study show that backscattering coefficients of radar scatterometer appear effective to estimate soil moisture content.