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luxS and smcR Quorum-Sensing System of Vibrio vulnificus as an Important Factor for In Vivo Survival  

SHIN NA-RI (Department of Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
BAEK CHANG-HO (Department of Life Science, College of Natural Science, Sogang University)
LEE DEOG-YONG (Department of Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
CHO YOUNG-WOOK (Department of Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
PARK DAE-KYUN (Department of Life Science, College of Natural Science, Sogang University)
LEE KO-EUN (Department of Life Science, College of Natural Science, Sogang University)
KIM KUN-SOO (Department of Life Science, College of Natural Science, Sogang University)
YOO HAN-SANG (Department of Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
Publication Information
Journal of Microbiology and Biotechnology / v.15, no.6, 2005 , pp. 1197-1206 More about this Journal
Abstract
Vibrio vulnificus is an opportunistic pathogen that causes a septicemia and expresses numerous virulence factors, in which luxS and smcR are genes encoding for components responsible for quorum-sensing regulation. In the present study, null mutants were constructed with lesions in each or both of these two genes from the V. vulnificus Vv$\Delta$Z strain, which is a lacZ$^{-}$ and chloramphenicol/streptomycin-resistant derivative of the wild-type ATCC29307 strain, and their phenotypes related to virulence were compared with those of the parental cells. $LD_{50}$ and histopathological findings of luxS-, smcR-, or luxS- smcR- deficient mutant were not different from those of the parent strain, a lacZ-deficient streptomycin-resistant strain in mice. However, time of death in mice was delayed, and numbers of bacteria survived in bloodstream after intraperitoneal injection in mice were decreased by mutation, especially luxS and smcR double mutant (VvSR$\Delta$ZSR). These phenomena were supported by increased serum sensitivity and delayed bacterial proliferation in both murine blood and iron-restricted medium. These results suggest that the luxS and luxR homologous genes in V. vulnificus could playa role in bacterial survival in host by enhancing proliferation and adjusting to changed environment.
Keywords
Vibrio vulnificus; luxS; smcR;
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1 Stintzi, A., K. Evans, J. M. Meyer, and K. Poole. 1998. Quorum-sensing and siderophore biosynthesis in Pseudomonas aeruginosa: lasR/lasI mutants exhibit reduced pyoverdine biosynthesis. FEMS Microbiol. Lett. 166: 341-345   DOI   ScienceOn
2 Zhang, L., P. J. Murphy, A. Kerr, and M. E. Tate. 1993. Agrobacterium conjugation and gene regulation by N-acyl- L-homoserine lactones. Nature 362: 446-448   DOI   ScienceOn
3 Bullen, J. J., P. B. Spalding, C. G. Ward, and J. M. Gutteridge. 1991. Hemochromatosis, iron and septicemia caused by Vibrio vulnificus. Arch. Intern. Med. 151: 1606-1609   DOI   ScienceOn
4 Chan, T. Y., D. P. Chow, K. C. Ng, K. W. Pan, and G. A. McBride. 1994. Vibrio vulnificus septicemia in a patient with liver cirrhosis. Southeast Asian J. Trop. Med. Public Health 25: 215-216
5 Eberhard, A., A. L. Burlingame, C. Eberhard, G. L. Kenyon, K. H. Nealson, and N. J. Oppenheimer. 1981. Structural identification of autoinducer of Photobacterium fischeri luciferase. Biochemistry 20: 2444-2449   DOI   ScienceOn
6 Gray, L. D. and A. S. Kreger. 1985. Purification and characterization of an extracellular cytolysin produced by Vibrio vulnificus. Infect. Immun. 48: 62-72
7 McDougald, D., S. A. Rice, and S. Kjelleberg. 2001. SmcR-dependent regulation of adaptive phenotypes in Vibrio vulnificus. J. Bacteriol. 183: 758-762   DOI   ScienceOn
8 Jobling, M. G. and R. K. Holmes. 1997. Characterization of hapR, a positive regulator of the Vibrio cholerae HA/ protease gene hap, and its identification as a functional homologue of the Vibrio harveyi luxR gene. Mol. Microbiol. 26: 1023-1034   DOI   ScienceOn
9 Kim, S. Y., S. E. Lee, Y. R. Kim, C. M. Kim, P. Y. Ryu, H. E. Choy, S. S. Chung, and J. H. Rhee. 2003. Regulation of Vibrio vulnificus virulence by the LuxS quorum-sensing system. Mol. Microbiol. 48: 1647-1664   DOI   ScienceOn
10 Kumamoto, K. S. and D. J. Vukich. 1998. Clinical infections of Vibrio vulnificus: A case report and review of the literature. J. Emergency Med. 16: 61-66   DOI   ScienceOn
11 Nealson, K. H. and J. W. Hastings. 1979. Bacterial bioluminescence: Its control and ecological significance. Microbiol. Rev. 43: 496-518
12 Oliver, J. D. 1989. Vibrio vulnificus, pp. 569-600. In Doyle, M. (ed.), Foodborne Bacterial Pathogens. Marcel-Dekker, New York, U.S.A
13 Park, J. W., S. N. Ma, E. S. Song, C. H. Song, M. R. Chae, B. H. Park, H. W. Rho, S. D. Park, and H. R. Kim. 1996. Pulmonary damage by Vibrio vulnificus cytolysin. Infect. Immun. 64: 2873-2876
14 Park, K. J., S. H. H. Kim, M. G. Kim, D. H. Chung, S. D. Ha, K. S. Kim, D. J. Jahng, and K. H. Lee. 2004. Functional complement of Escherichia coli by the rpoS gene of the foodborne pathogenic Vibrio vulnificus. J. Microbiol. Biotechnol. 14: 1063-1066
15 Shao, C. P. and L. I. Hor. 2000. Metalloprotease is not essential for Vibrio vulnificus virulence in mice. Infect. Immun. 68: 3569-3573   DOI   ScienceOn
16 Shao, C. P. and L. I. Hor. 2001. Regulation of metalloprotease gene expression in Vibrio vulnificus by a Vibrio harveyi LuxR homologue. J. Bacteriol. 183: 1369-1375   DOI   ScienceOn
17 Testa, J., L. W. Daniel, and A. S. Kreger. 1984. Extracellular phospholipase A2 and lysophospholipase produced by Vibrio vulnificus. Infect. Immun. 59: 192-197
18 Wright, A. C., L. M. Simpson, and J. D. Oliver. 1981. Role of iron in the pathogenesis of Vibrio vulnificus infections. Infect. Immun. 34: 503-507
19 Muench, K. H. 1989. Hemochromatosis and infection: Alcohol and iron, oysters and sepsis. Am. J. Med. 87: 40N-43N   DOI   ScienceOn
20 Rhee, J. E., H. M. Ju, U. Y. Park, B. C. Park, and S. H. Choi. 2004. Identification of the Vibrio vulnificus cadC and evaluation of its role in acid tolerance. J. Microbiol. Biotechnol. 14: 1093-1098
21 Maeda, H. and T. Yamamoto. 1996. Pathogenic mechanisms induced by microbial proteases in microbial infection. Biol. Chem. Hoppe-Seyler 377: 217-226
22 Swift, S., A. V. Karlyshev, L. Fish, E. L. Durant, M. K. Winson, S. R. Chhabra, P. Williams, S. Macintyre, and G. S. Stewart. 1997. Quorum sensing in Aeromonas hydrophilia and Aeromonas salmonicida: Identification of the LuxRI homologs AhyRI and AsaRI and their cognate Nacylhomoserine lactone signal molecules. J. Bacteriol. 179: 5271-5281   DOI
23 Wright, A. C., J. L. Powell, J. B. Kaper, and J. G. Morris. 2001. Identification of a group 1-like capsular polysaccharide operon for Vibrio vulnificus. Infect. Immun. 69: 6893-6901   DOI   ScienceOn
24 Schauder, S., K. Shokat, M. G. Surette, and B. L. Bassler. 2001. The LuxS family of bacterial autoinducers: Biosynthesis of a novel quorum-sensing signal molecule. Mol. Microbiol. 41: 463-476   DOI   ScienceOn
25 Keen, N. T., S. Takami, D. Kobayashi, and D. Trollinger. 1988. Improved broad-host range plasmids for DNA cloning in Gram-negative bacteria. Gene 70: 191-197   DOI   ScienceOn
26 Poper, K. R., S. Beck von Bodman, and S. K. Farrand. 1993. Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Nature 362: 448-450   DOI   ScienceOn
27 Skorupski, K. and R. K. Taylor. 1996. Positive selection vectors for allelic exchange. Gene 169: 47-52   DOI   ScienceOn
28 Maruo, K., T. Akaike, T. Ono, and H. Maeda. 1998. Involvement of bradykinin generation in intravascular dissemination of Vibrio vulnificus and prevention of invasion by a bradykinin antagonist. Infect. Immun. 66: 866-869
29 McCarter, L. L. 1998. OpaR, a homolog of Vibrio harveyi LuxR, controls opacity of Vibrio parahaemolyticus. J. Bacteriol. 180: 3166-3173
30 Rhee, J. E., J. H. Lee, H. S. Jeong, U. Y. Park, D. H. Lee, G. J. Woo, S. I. Moyoshi, and S. H. Choi. 2003. Evidence that temporally alternative expression of the Vibrio vulnificus elastase prevents proteolytic inactivation of hemolysin. J. Microbiol. Biotechnol. 13: 1021-1026
31 Maeda, H., T. Akaike, Y. Sakata, and K. Maruo. 1993. Role of bradykinin in microbial infection: Enhancement of septicemia by microbial proteases and kinin. Agents Actions Suppl. 42: 159-165
32 Beck van Bodman, S. and S. K. Farrand. 1995. Capsular polysaccharide biosynthesis and pathogenicity in Erwinia stewartii require induction by an N-acylhomoserine lactone autoinducer. J. Bacteriol. 177: 5000-5008   DOI
33 Bainton, N. J., P. Stead, S. R. Chhabra, B. W. Bycroft, G. P. Salmond, G. S. Stewart, and P. Williams. 1992. N-3-oxohexanoyl-L-homoserine lactone regulates carbapenem antibiotic production in Erwinia carotovora. Biochem. J. 288: 997-1004   DOI
34 Sambrook, J., E. F. Fritsch, and T. A. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. 2nd Ed. Cold Harbor Laboratory Press, Cold Spring Harbor, New York, U.S.A
35 Paranjpye, R. N., J. C. Lara, J. C. Pepe, C. M. Pepe, and M. S. Strom. 1998. The type IV leader peptidase/N-methyltransferase of Vibrio vulnificus controls factors required for adherence to HEp-2 cells and virulence in ironoverloaded mice. Infect. Immun. 66: 5659-5668
36 Brennt, C. E., A. C. Wright, S. K. Dutta, and J. G. Morris, Jr. 1991. Growth of Vibrio vulnificus in serum from alcoholics: Association with high transferring iron saturation. J. Infect. Dis. 164: 1030-1032   DOI   ScienceOn
37 Davies, D. G., M. R. Parsek, J. P. Pearson, B. H. Iglewski, J. W. Costerton, and E. P. Greenberg. 1998. The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 280: 295-298   DOI   PUBMED   ScienceOn
38 Jeong, H. S., J. E. Rhee, J. H. Lee, H. K. Choi, D. I. Kim, M. H. Lee, S. J. Park, and S. H. Choi. 2003. Identification of Vibrio vulnificus lrp and its influence on survival under various stress. J. Microbiol. Biotechnol. 13: 159-163
39 Zhu, J., M. B. Miller, R. E. Vance, M. Dziejman, B. L. Bassler, and J. J. Mekalanos. 2002. Quorum-sensing regulators control virulence gene expression in Vibrio cholerae. Proc. Natl. Acad. Sci. USA 99: 3129-3134
40 Lewenza, S., B. Conway, E. P. Greenberg, and P. A. Sokol. 1999. Quorum sensing in Burkholderia cepacia: Identification of the LuxRI homologs CepRI. J. Bacteriol. 181: 748-756
41 De Lorenzo, V. and K. N. Timmis. 1994. Analysis and construction of stable phenotypes in Gram-negative bacteria with Tn5- and Tn10-derived minitransposons. Methods Enzymol. 235: 386-405   DOI
42 McDougald, D., S. A. Rice, and S. Kjelleberg. 2000. The marine pathogen Vibrio vulnificus encodes a putative homologue of the Vibrio harveyi regulatory gene, luxR: A genetic and phylogenetic comparison. Gene 248: 213-221   DOI   PUBMED   ScienceOn
43 Simpson, L. M. and J. D. Oliver. 1987. Ability of Vibrio vulnificus to obtain iron from transferring and other ironbinding proteins. Curr. Microbiol. 15: 155-157   DOI
44 Reed, L. J. and H. Muench. 1938. A simple method of estimating fifty percent endpoints. Am. J. Hyg. 27: 493-497
45 Hanne, L. F. and R. A. Finkelstein. 1982. Characterization and distribution of the hemagglutinins produced by Vibrio cholerae. Infect. Immun. 36: 209-214
46 Engebrecht, J., K. Nealson, and M. Silverman. 1983. Bacterial bioluminescence: Isolation and genetic analysis of functions from Vibrio fischeri. Cell 32: 773-781   DOI   ScienceOn