• Title/Summary/Keyword: VP4

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Evaluation of Scatter Radiation in Digital Radiological Condition by using Photostimulated Luminescence (BaFBr:$Eu^{2+}$) (휘진성 형광체 (BaFBr:$Eu^{2+}$)를 이용한 영상의학분야에서 산란선 특성에 관한 평가)

  • Min, Jung-Whan;Han, Seong-Gyu;Kim, Jung-Min;Lee, Joo-Ah;Kim, Ki-Won;Jeong, Hoi-Woun
    • Journal of radiological science and technology
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    • v.37 no.2
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    • pp.85-91
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    • 2014
  • The purpose of this study is evaluated scatter radiation in digital radiological condition by using photo-stimulated luminescence (BaFBr:$Eu^{2+}$). Experiment condition changed kVp (from 50 kVp to 120 kVp), filed size (from $4{\times}4cm^2$ to $26{\times}26cm^2$) and phantom thickness (from 1 cm to 15 cm). This method was analysed ImageJ and characteristic curve of CR. This results was scatter radiation to primary radiation ratio increased from 50 kVp to 70 kVp, and it was fixed at over 80 kVp. The scatter radiation to primary radiation ratio are increased according to increasing the ratio of field size. Scatter radiation is also increased by increasing the phantom thickness.

Application of AuNPs immobilized on UV Cross-linked P4VP Thin Film as pH Nanosensors (pH 나노센서로의 응용을 위한 UV-가교 P4VP 박막에 고정한 금 나노입자의 특성)

  • Kim, Min-Sung;Jeong, Yeon-Tae
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.21 no.11
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    • pp.1010-1018
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    • 2008
  • In this report, we describe the use of gold nanoparticles (AuNPs) immobilized on pH. responsive, cross-linked poly(4-vinylpyridine) (P4VP) thin films, as a potential application for pH nanosensors. The methodology is based on the variation in surface plasmon resonance of immobilized AuNPs with changing the interparticle distances, caused by the swelling/deswelling of the pH responsive P4VP polymer films. The change in optical properties of the immobilized AuNPs in response to the pH of surrounding media was investigated by a simple yet powerful tool; UV-vis absorption spectroscopy. The swelling of the P4VP chains at pH 2 causes an increase in the interparticle distances of immobilized AUNPS ($\sim20nm$) and hence leads to a blue shift of 48 nm in their surface plasmon resonance band peak. On the other hand, when the surrounding media was altered from pH 2 to 10, a red shift of absorption maxima was observed. The changes were rapid, and the effect was reversible. This system could prove to be useful in fabricating nanosensors for detecting the pH or pH changes of surrounding aqueous medium.

Distribution of Disease Severity and Group A Rotavirus Genotypes (VP7 & VP4) in Children with Acute Rotavirus Gastroenteritis (급성 로타바이러스 장염 소아에서 질환의 중증도와 로타바이러스 VP7 & VP4 유전형의 분포에 대한 연구)

  • Oh, Hyun-Ju;Kang, Hyun-Sik;Kang, Ki-Soo;Kim, Youn-Woo;Hong, Jung-Yun;Shin, Kyung-Sue;Lee, Jin-Sook;Lee, Su-Hyun;Lee, Keun-Hwa;Cho, Moon-Je;Suh, Dong-In
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.14 no.2
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    • pp.148-154
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    • 2011
  • Purpose: We aimed to study the distribution of rotavirus genotypes (VP7 and VP4) and disease severity of rotavirus gastroenteritis prevalent in our community. Methods: Stool samples were collected from 156 children who were hospitalized with rotavirus gastroenteritis from December 2007 to June 2008. The disease severity of all patients was scored using the Vesikari scale. After extraction of ds-RNA of the rotavirus, cDNA synthesis using reverse transcription and polymerase chain reaction (RT-PCR) and multiplex PCR was performed. Following this, the final identification of genotypes was performed. Results: Of the 156 samples, VP7(G) and VP4(P) genotypes were identified in 147 (94.2%) and 140 (89.7%) samples, respectively. G1 (116 of 147 samples; 78.9%) and P[8] (137 of 140 samples; 97.9%) were the most prevalent, respectively. Of the 138 samples identified of combination types of VP7 and VP4, G1P[8] (111 samples; 80.4%) was the most prevalent. Other combination types varied with very low distribution rates. 9.4% of genotypes were not included in the new vaccines. The disease severity score was $11.8{\pm}3.3$ ($mean{\pm}2SD$). The distribution of disease severity was mild or moderate in 37.8% and severe in 62.2% of patients. Conclusion: The most prevalent genotype combination of rotavirus was G1P[8] and genotypes not included in the vaccines represented 9.4% in our community. Disease severity distribution of hospitalized children with rotavirus gastroenteritis was higher in the severe than in the mild and moderate categories.

Effectiveness Evaluation of the Tube Voltage Measurement by using Additional Filter (부가필터를 이용한 kVp 측정의 실효성 평가)

  • Hwang, Jun-Ho;Lee, Kyung-Ho;Choi, Yoon-Bong;Kang, Byung-Sam
    • Journal of radiological science and technology
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    • v.38 no.4
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    • pp.355-363
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    • 2015
  • Advancement in the medical field provides an opportunity for the development of medical equipment and also enable accurate diagnosis for the inspection and the treatment of diseases. The aging of equipment due to the frequent operation produce uncertainty in the patient exposure dose, so a quality control was implemented by establishing a safety management system on a regular basis. The x-ray tube voltage (kVp), which is directly involved in the patient exposure dose and the life of the equipment, needs periodic performance for the quality control, but it has a limitation due to the time and the cost. In this study, we proposed a new method for measuring the kVp with ease by using the Y and Cu to solve the problem of the time and cost. We also evaluated the usefulness and the effectiveness of the new method and its accuracy through the kVp measurement. After securing the condition, the amount of the tube current and evaluating the usefulness and effectiveness of kVp measured using Y and Cu. The density range used was at 0.5-1.0, kVp was in the range of ${\pm}10%$, then, we recorded the change in concentration as % of Y and Cu. This experiment showed that when Cu and Y was at 75 kVp, concentration was the same, and when the kVp was changed to 65 ~ 85 %, a gradual increase in concentration to 85 ~ 110 % was noted.

Studies on the VP4 and VP7 Genes of Bovine Rotaviruses from Field Samples Using RT-PCR and RFLP Analysis (RT-PCR과 RFLP법을 이용한 국내 소 로타바이러스 VP4 및 VP7 유전자의 특성 규명)

  • Jeon, Seong-Jin;Chang, Chung-Ho;Chung, Chung-Won;Kim, Won-Yong;Kang, Shien-Young
    • The Journal of Korean Society of Virology
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    • v.28 no.2
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    • pp.165-174
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    • 1998
  • Characterizations of the VP4 (P type) and VP7 (G type) genes of Korean isolates of bovine rotavirus were performed using RT-PCR/RFLP and nucleotide sequencing analysis. After RT-PCR amplification of partial length (1094bp) of the VP4 and full length (1062bp) of the VP7 genes, amplified PCR products were digested with restriction endonucleases and digestion patterns were compared with those of reference rotaviruses. With the VP4 genes, four RFLP (A-D) profiles were observed; three (A, Band C) were the same as those of bovine rotavirus NCDV (P[1]), IND (P[5]) and B223 (P[11]), respectively. Profile D was the same as that of porcine rotavirus OSU (P[7]). With the VP7 genes, five RFLP profiles (I-V) were observed; three of them (I, II and III) were the same as those of bovine rotavirus NCDV (G6), Cody 1-801 (G8), and B223 (G10), respectively. Profile IV and V were atypical to those of reference bovine rotaviruses used in this study. These two profiles were identified as G6 and G5, respectively, after analyzing and comparing the nucleotide sequences. The G typing analysis revealed that 61.9% (26/42) were G6, which included G6 subtype; 28.6% (12/42) were G5; 7.1% (3/42) were G10; 2.4% (1/42) were G8. The P typing analysis revealed that 54.8% (23/42) were P[5]; 28.6% (12/42) were P[7]; 11.8% (5/42) were [11]; 4.8% (2/42) were P[1]. Our results showed that G6/P[5] were the most prevalent rotaviruses in diarrheic calves in Korea. Also, this is the first report that G5/P[7] rotaviruses were identified from cattle with diarrhea.

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Backup Path to be satisfied QoS in ATM Network (ATM 망에서 QoS를 보장하는 백업 경로 설정)

  • 이동욱;이대영
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.4A
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    • pp.496-501
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    • 2000
  • In this thesis, we propose more confidence required backup-VP allocation. First, we assigned backup VP considering Reserved resource on realtime QoS with each QoS parameter priority and then allocated backup VP of non-realtime QoS. The proposed algorithm provides more confidential backup VP by priority method for relative high cost realtime QoS error.

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Production of Recombinant Rotavirus Capsid Protein VP7 from Stably Transformed Drosophila melanogaster S2 Cells

  • Park, Jong-Hwa;Chang, Kyung-Hwa;Lee, Youn-Hyung;Kim, Hae-Yeong;Yang, Jai-Myung;Chung, In-Sik
    • Journal of Microbiology and Biotechnology
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    • v.12 no.4
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    • pp.563-568
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    • 2002
  • Stably transformed Drosophila melanogaster 52 cells producing recombinant VP7 were obtained, and recombinant VP7 expression was confirmed by Western blot analysis. The molecular weight of recombinant VP7 expressed in 52 cells was approximately 35.5 kDa, and 75% of the total VP7 produced was present in the medium. Recombinant VP7 contained N-linked glycosylated oligosaccharides. Aprotinin, leupeptin, and polyvinylpyrrolidone did not have any noticeable effect on recombinant VP7 production; however, DMSO and sodium butyrate increased its production by 120% and 60%, respectively.

Implementation of OPNET-based simulation model for the performance evaluation of ATM VP Transit network (ATM VP 중계망의 성능 시험을 위한 OPNET 기반의 시뮬레이션 모델 구현)

  • 구수용;김영탁
    • Journal of the Korea Society for Simulation
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    • v.8 no.4
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    • pp.125-136
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    • 1999
  • In the forthcoming public ATM/B-ISDN, the efficient resource management with pre-planned transit networking which public domain NNI signaling is essential to maintain high network utilization and to assure QoS to the multimedia service users. For this purpose the transit networks must be managed according to the bearer service capability which is defined by ATM Forum and ITU-T. In this paper, we introduce an implementation of ATM transit networking with ATM VP-XC(Virtual Path cross-connect) and US(Network Management System). The functions of ATM VP-XC and NMS have been simulated with OPNET 6.1 modules. We implemented the F4 OAM functions of ATM VP connection according to the ITU-T 1.610 recommendation. Also, the ATM VP transit networking is managed by the NMS according to the connection management architecture of the TWN(Telecommunications Management Network) /TINA ( Telecommunications Information Networking Architecture).

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Scanning Electron Microscopic Study of the Developing Vallate Papillae in the Korean Native Goat (Capra hircus)

  • Cho, Gyuhyen;Kim, Munki;Lee, Sijoon;Kim, Chongsup;Won, Chungkil
    • Development and Reproduction
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    • v.20 no.4
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    • pp.283-288
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    • 2016
  • The purpose of the present study was to investigate the morphological characteristics of the developing vallate papillae (VP) of Korean native goats using scanning electron microscopy. In prenatal development of the VP, primordia of the VP were observed and the moat was shallowly spread in 60-day-old fetuses. The moat of the vallate papillae was shallowly spread and still undifferentiated in 90-day-old fetuses. The trench wall of the moat of the VP was well developed in 120-day- old fetuses. In neonates, the moat of the VP was more widely and deeply engraved and VP were developed as completely as those of adults. In postnatal development, VP were observed to have continually increased in size with slight morphological changes until 90-days after birth. Taste pores of the VP were shaped like flower leaves in 120-days after birth. The microridges and microplicaes were well developed on the epithelial surface of the VP in goats ranging from 120-day-old fetuses to 120-day-old postnatal animals. These results suppose that the sensing ability for gestation of VP was already well developed by the time of its birth and VP were differentiated into a variety of different shape and size during development.

Characterization of G9 genotype porcine rotavirus isolated in Korea (국내 양돈장에서 분리한 G9형 돼지로타바이러스의 특성)

  • Wang, Jun-Hui;Lee, Seung-Chul;Kang, Shien-Young
    • Korean Journal of Veterinary Service
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    • v.35 no.2
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    • pp.83-90
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    • 2012
  • Porcine rotaviruses are the most common causes of viral gastroenteritis in piglets around the world. The major G genotypes of porcine rotaviruses causing diarrhea were G4, G5 and G11 genotypes. Recently, G9 genotype rotaviruses were problemed at swine farms and frequently recognized from diarrheic piglets. In this study, a porcine rotavirus (PoRV-1) was isolated from piglet showing diarrhea using MA104 cells and confirmed as rotavirus by electron microscopy, genomic RNA electropherotyping and indirect immunofluorescence antibody tests. The nucleotide sequence of the VP7 gene of PoRV-1 was determined and compared with those of other genotype rotavirus strains from other parts of the world. Also, the nucleotide sequences of VP4, VP6 and NSP4 genes of PoRV-1 were determined and compared with those of other rotavirus strains from other countries. The results showed that the PoRV-1 isolate belonged to the G9 genotype and the P, I and E genotypes of PoRV-1 were P[23], I5 and E1, respectively. The Korean G9 PoRV-1 isolate and its nucleotide sequence data would be usefully used for the development of porcine rotavirus vaccines in near future.