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Production of Recombinant Rotavirus Capsid Protein VP7 from Stably Transformed Drosophila melanogaster S2 Cells

Park, Jong-Hwa (Department of Genetic Engineering, Kyung Hee University)
Chang, Kyung-Hwa (Department of Genetic Engineering, Kyung Hee University)
Lee, Youn-Hyung (Department of Genetic Engineering, Kyung Hee University)
Kim, Hae-Yeong (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University)
Yang, Jai-Myung (Department of Life Science, Sogang University)
Chung, In-Sik (Department of Genetic Engineering, Kyung Hee University, Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University)

Publication Information

Journal of Microbiology and Biotechnology / v.12, no.4, 2002 , pp. 563-568 More about this Journal

Abstract

Stably transformed Drosophila melanogaster 52 cells producing recombinant VP7 were obtained, and recombinant VP7 expression was confirmed by Western blot analysis. The molecular weight of recombinant VP7 expressed in 52 cells was approximately 35.5 kDa, and 75% of the total VP7 produced was present in the medium. Recombinant VP7 contained N-linked glycosylated oligosaccharides. Aprotinin, leupeptin, and polyvinylpyrrolidone did not have any noticeable effect on recombinant VP7 production; however, DMSO and sodium butyrate increased its production by 120% and 60%, respectively.

Keywords

Drosophila melanogaster S2 cell; rotavirus capsid protein VP7; DMSO; sodium butyrate;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)
Times Cited By Web Of Science : 2 (Related Records In Web of Science)

Reference
Cited By KSCI

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