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Detection and Identification of Vibrio Species Using Whole-Cell Protein Pattern Analysis

  • Lee, Chae-Yoon;Hong, Yeun;Ryu, Jio;Kim, Young-Rok;Oh, Sang-Suk;Lee, Soon-Ho;Hwang, In-Gyun;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
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    • v.22 no.8
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    • pp.1107-1112
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    • 2012
  • Outbreaks of foodborne diseases associated with Vibrio species such as V. parahaemolyticus, V. vulnificus, and V. cholerae frequently occur in countries having a dietary habit of raw seafood consumption. For rapid identification of different Vibrio species involved in foodborne diseases, whole-cell protein pattern analysis for 13 type strains of 12 Vibrio species was performed using SDS-PAGE analysis. Pathogenic Vibrio species such as V. parahaemolyticus, V. vulnificus, V. cholerae, V. alginolyticus, V. fluvialis, and V. mimicus were included in the 12 Vibrio species used in this study. Each of the 12 Vibrio species showed clearly specific band patterns of its own. Two different strains of V. parahaemolyticus showed two different SDS-PAGE whole-cell protein patterns, giving the possibility of categorizing isolated strains in the same V. parahaemolyticus species into two subgroups. The 36 Vibrio isolates collected from sushi restaurants in Busan were all identified as V. parahaemolyticus by comparing their protein patterns with those of Vibrio type strains. The identified isolates were categorized into two different subgroups of V. parahaemolyticus. The whole-cell protein pattern analysis by SDS-PAGE can be used as a specific, rapid, and simple identification method for Vibrio spp. involved in foodborne diseases at the subspecies level.

THE DESIGN OF AN EFFICIENT LOAD BALANCING ALGORITHM EMPLOYING BLOCK DESIGN

  • Chung, Il-Yong;Bae, Yong-Eun
    • Journal of applied mathematics & informatics
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    • v.14 no.1_2
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    • pp.343-351
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    • 2004
  • In order to maintain load balancing in a distributed system, we should obtain workload information from all the nodes on network. This processing requires $O(v^2)$ communication overhead, where v is the number of nodes. In this paper, we present a new synchronous dynamic distributed load balancing algorithm on a (v, k + 1, 1)-configured network applying a symmetric balanced incomplete block design, where $v\;=\;k^2$\;+\;k\;+\;1$. Our algorithm needs only $O(\sqrt[v]{v})$ communication overhead and each node receives workload information from all the nodes without redundancy. Therefore, load balancing is maintained since every link has the same amount of traffic for transferring workload information.

FVF-Based Low-Dropout Voltage Regulator with Fast Charging/Discharging Paths for Fast Line and Load Regulation

  • Hinojo, Jose Maria;Lujan-Martinez, Clara;Torralba, Antonio;Ramirez-Angulo, Jaime
    • ETRI Journal
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    • v.39 no.3
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    • pp.373-382
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    • 2017
  • A new internally compensated low drop-out voltage regulator based on the cascoded flipped voltage follower is presented in this paper. Adaptive biasing current and fast charging/discharging paths have been added to rapidly charge and discharge the parasitic capacitance of the pass transistor gate, thus improving the transient response. The proposed regulator was designed with standard 65-nm CMOS technology. Measurements show load and line regulations of $433.80{\mu}V/mA$ and 5.61 mV/V, respectively. Furthermore, the output voltage spikes are kept under 76 mV for 0.1 mA to 100 mA load variations and 0.9 V to 1.2 V line variations with rise and fall times of $1{\mu}s$. The total current consumption is $17.88{\mu}V/mA$ (for a 0.9 V supply voltage).

INJECTIVELY DELTA CHOOSABLE GRAPHS

  • Kim, Seog-Jin;Park, Won-Jin
    • Bulletin of the Korean Mathematical Society
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    • v.50 no.4
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    • pp.1303-1314
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    • 2013
  • An injective coloring of a graph G is an assignment of colors to the vertices of G so that any two vertices with a common neighbor receive distinct colors. A graph G is said to be injectively $k$-choosable if any list $L(v)$ of size at least $k$ for every vertex $v$ allows an injective coloring ${\phi}(v)$ such that ${\phi}(v){\in}L(v)$ for every $v{\in}V(G)$. The least $k$ for which G is injectively $k$-choosable is the injective choosability number of G, denoted by ${\chi}^l_i(G)$. In this paper, we obtain new sufficient conditions to be ${\chi}^l_i(G)={\Delta}(G)$. Maximum average degree, mad(G), is defined by mad(G) = max{2e(H)/n(H) : H is a subgraph of G}. We prove that if mad(G) < $\frac{8k-3}{3k}$, then ${\chi}^l_i(G)={\Delta}(G)$ where $k={\Delta}(G)$ and ${\Delta}(G){\geq}6$. In addition, when ${\Delta}(G)=5$ we prove that ${\chi}^l_i(G)={\Delta}(G)$ if mad(G) < $\frac{17}{7}$, and when ${\Delta}(G)=4$ we prove that ${\chi}^l_i(G)={\Delta}(G)$ if mad(G) < $\frac{7}{3}$. These results generalize some of previous results in [1, 4].

Improvement of COMS land surface temperature retrieval algorithm by considering diurnal variation of air temperature (기온의 일 변동을 고려한 COMS 지표면온도 산출 알고리즘 개선)

  • Choi, Youn-Young;Suh, Myoung-Seok
    • Korean Journal of Remote Sensing
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    • v.32 no.5
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    • pp.435-452
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    • 2016
  • Land Surface Temperature (LST) has been operationally retrieved from the Communication, Ocean, and Meteorological Satellite (COMS) data by the spilt-window method (CSW_v2.0) developed by Cho et al. (2015). Although the CSW_v2.0 retrieved the LST with a reasonable quality compared to the Moderate Resolution Imaging Spectroradiometer (MODIS) LST data, it showed a relatively poor performance for the strong inversion and lapse rate conditions. To solve this problem, the LST retrieval algorithm (CSW_v2.0) was updated using the simulation results of radiative transfer model (MODTRAN 4.0) by considering the diurnal variations of air temperature. In general, the upgraded version, CSW_v3.0 showed a similar correlation coefficient between the prescribed LSTs and retrieved LSTs (0.99), the relatively smaller bias (from -0.03 K to-0.012 K) and the Root Mean Square Error (RMSE) (from 1.39 K to 1.138 K). Particularly, CSW_v3.0 improved the systematic problems of CSW_v2.0 that were encountered when temperature differences between LST and air temperature are very large and/or small (inversion layers and superadiabatic lapse rates), and when the brightness temperature differences and surface emissivity differences were large. The bias and RMSE of CSW_v2.0 were reduced by 10-30% in CSW_v3.0. The indirect validation results using the MODIS LST data showed that CSW_3.0 improved the retrieval accuracy of LST in terms of bias (from -0.629 K to -0.049 K) and RMSE (from 2.537 K to 2.502 K) compared to the CSW_v2.0.

Medullary Sponge Kidney Detected in the Pediatric Age (소아 연령에서 발견된 medullary sponge kidney)

  • Chung, Woo Yeong;Cho, Min Hyun;Gu, Young-Ran;Leem, Sun-Hee;Cheong, Hae Il
    • Childhood Kidney Diseases
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    • v.16 no.2
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    • pp.109-114
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    • 2012
  • Purpose: Medullary sponge kidney (MSK) is a rare congenital disease characterized by diffuse ectasia or dilatation of precalyceal collecting tubules. MSK incidence and prevalence in the general population is uncertain and only a few patients are reported especially in the pediatric age. There has been increasing reports of patients with MSK who have other malformative disorders. Also several case reports concerning about etiological association of some genes. Methods: Collaborative study through nation-wide survey was done to investigate the incidence and etiological association of some genes such as GDNF gene, ATP6V1B1, ATP6V0A4 gene in developing MSK in Korean children. Results: Four cases of MSK who have various other malformative disorders were collected. There are no mutations of GDNF gene, ATP6V1B1, ATP6V0A4 gene in all patients. Conclusion: MSK is one of the very rare diseases in pediatric age. The etiological association of GDNF gene, ATP6V1B1, ATP6V0A4 gene in developing MSK in Korean children is not proved.

Benzyl Alcohol Oxidation over H5PMo10V2O40 Catalyst Chemically Immobilized on Sulfur-containing Mesoporous Carbon (황이 포함된 중형기공성 탄소에 화학적으로 고정화된 H5PMo10V2O40 촉매 상에서 Benzyl Alcohol 산화반응)

  • Gim, Min Yeong;Kang, Tae Hun;Choi, Jung Ho;Song, In Kyu
    • Korean Chemical Engineering Research
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    • v.54 no.3
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    • pp.419-424
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    • 2016
  • $H_5PMo_{10}V_2O_{40}$ ($PMo_{10}V_2$) catalyst chemically immobilized on sulfur-containing mesoporous carbon (S-MC) was prepared, and it was applied to the benzyl alcohol oxidation reaction. S-MC was synthesized by a templating method using SBA-15 and p-toluenesulfonic acid as a templating agent and a carbon precursor, respectively. S-MC was then modified to have a positive charge, and thus, to provide sites for the immobilization of $PMo_{10}V_2$. By taking advantage of the overall negative charge of $[PMo_{10}V_2O4_{40}]^{5-}$, $PMo_{10}V_2$ catalyst was immobilized on the S-MC support as a charge matching component. It was revealed that $PMo_{10}V_2$ species were finely and molecularly dispersed on the S-MC via chemical immobilization. In the vapor-phase oxidation of benzyl alcohol, $PMo_{10}V_2$/S-MC catalyst showed higher conversion of benzyl alcohol and higher yield for benzaldehyde and benzoic acid than unsupported $PMo_{10}V_2$ catalyst. The enhanced catalytic performance of $PMo_{10}V_2$/S-MC was due to fine dispersion of $PMo_{10}V_2$ species on the S-MC via chemical immobilization.

Characteristics of Insulation Diagnosis and Failure in 6.6 kV Motor Stator Windings (6.6 kV 전동기 고정자 권선의 절연진단과 절연파괴 특성)

  • Kim, Hee-Dong;Kong, Tae-Sik
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.25 no.4
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    • pp.309-314
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    • 2012
  • To assess the condition of stator insulation, nondestructive and overpotential tests were performed on four high voltage motors. The stator windings under these tests have nominal ratings of 6.6 kV. After completing nondestructive tests, the AC overvoltage applied to the stator windings was gradually increasing until insulation failure in order to obtain the breakdown voltage. No. 1, No. 2, No. 3 and No. 4 of 6.6 kV motors failed near rated voltage of 18.4 kV, 19.8 kV, 19.7 kV and 21.7 kV, respectively. The breakdown voltage of four motors was higher that expected for good quality coils(14.2 kV) in 6.6 kV motors. Almost all of failures were located in a line-end coil at the exit from the core slot. The breakdown voltages and the types of defects showed strong relation to the stator insulation tests such as in the case of AC current, dissipation factor($tan{\delta}$) and partial discharge magnitude.

Proposals of Reliable Shapes of Supplementary V-ties for Section Jacketing Method of Columns (기둥의 단면 확대보강을 위한 V-타이 보조 띠철근의 형상 제시)

  • Kwon, Hyuck-Jin;Yang, Keun-Hyeok;Sim, Jae-Il
    • Journal of the Korea Institute of Building Construction
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    • v.18 no.2
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    • pp.99-107
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    • 2018
  • The objective of the present study is to propose a reliable shape of supplementary V-ties in the section jacketing approach for seismic strengthening of reinforced concrete columns. A total of 24 pull-out specimens were prepared. The test parameters selected with regard to bond strength of V-ties were the shape of V-ties, embedment length of V-tie legs, and compressive strength of concrete. The measured bond strength of V-ties with different shapes were compared with that of the conventional V-ties and predictions using CEB-FIP equation. Ultimately, V-ties with pressed end-details at their legs could be recommended for the supplementary lateral reinforcement of strengthening columns with jacketing thickness less than the embedment length [= max (75mm, $6d_b$)] of conventional V-ties, where $d_b$ is the diameter of the reinforcing bar used for V-ties.

Improved Rate Capability of Li/Li3V2(PO4)3 Cell for Advanced Lithium Secondary Battery

  • Lim, Hyun-He;Cho, A-Ra;Sivakumar, Nagarajan;Kim, Woo-Seong;Yoon, Won-Sub;Lee, Yun-Sung
    • Bulletin of the Korean Chemical Society
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    • v.32 no.5
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    • pp.1491-1494
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    • 2011
  • Lithium vanadium phosphate, $Li_3V_2(PO_4)_3$ was prepared by a simple solid state route. It was found that making a fine powder of $Li_3V_2(PO_4)_3$ by the mechanical milling is very effective for increasing the insertion/extraction of lithium from $Li_3V_2(PO_4)_3$ structure. In charge/discharge test, the ball-milled $Li_3V_2(PO_4)_3$ sample exhibited a higher initial discharge capacity of 174 mAh/g in the voltage range of 3.0-4.8 V, compared with pure $Li_3V_2(PO_4)_3$ sample (152 mAh/g). Furthermore, the ball-milled $Li_3V_2(PO_4)_3$ presented not only higher cycle retention rate after 50 cycles, but also better rate capability compared with pure sample in the whole region (0.1-7 C).