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Development of Magnetically Separable Immobilized Lipase by Using Cellulose Derivatives and Their Application in Enantioselective Esterification of Ibuprofen

  • Lee, Go-Woun;Joo, Hong-Il;Kim, Jung-Bae;Lee, Jung-Heon
    • Journal of Microbiology and Biotechnology
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    • v.18 no.3
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    • pp.465-471
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    • 2008
  • Highly active, stable, and magnetically separable immobilized enzymes were developed using carboxymethyl cellulose (CMC) and diethylaminoethyl cellulose DEAE-C; hereafter designated "DEAE" as supporting materials. Iron oxide nanoparticles penetrated the micropores of the supporting materials, rendering them magnetically separable. Lipase (LP) was immobilized on the surface of the supporting materials by using cross-linked enzyme aggregation (CLEA) by glutaraldehyde. The activity of enzyme aggregates coated on DEAE was approximately 2 times higher than that of enzyme aggregates coated on CMC. This is explained by the fact that enzyme aggregates with amine residues are more efficient than those with carboxyl residues. After a 96-h enantioselective ibuprofen esterification reaction, 6% ibuprofen propyl ester was produced from the racemic mixture of ibuprofen by using DEAE-LP, and 2.8% using CMC-LP.

Identification of Soil Stiffness Using Forced Vibration Test Data (강제진동시험자료를 사용한 지반의 강성계수 추정)

  • 최준성;이종세;김동수;이진선
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2002.03a
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    • pp.101-108
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    • 2002
  • This paper presents an input and system identification technique for a free-field system using forced vibration data. Identification is carried out on geotechnical experiment site at Yong-jong Island where Inchon International Airport being constructed. The identified quantities are the input load as well as the shear moduli of the free-field soil regions. The dynamic response analysis on the free-field system is carried out using the finite element method incorporating the infinite element formulation fur the unbounded layered soil medium. The criterion function for the parameter estimation is constructed using the frequency response amplitude ratios of the dynamic responses measured at several points of the free-field, so that the information on the input loading may be excluded. The constrained steepest descent method is employed to obtain the revised parameters. The simulated dynamic responses using the identified parameters and input load show excellent agreements with the measured responses.

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On a Performance Comparison of Pitch Search Algorithms with the Correlation Properties for the CELP Vocoder (상관관계 특성을 이용한 CELP 보코더의 피치검색시간 단축법의 비교)

  • 김대식
    • Proceedings of the Acoustical Society of Korea Conference
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    • 1994.06c
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    • pp.188-194
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    • 1994
  • Code excited linear prediction speech coders exhibit good performance at data rates as low as 4800bps. But the major drawback to CELP type coders is their large computational requirements. Therefore, in this paper a comparative performance study of three pitch searching algorithms for the CELP vocoder was conducted. For each of the algorithms, a standard pitch searching algorithm was used by the full pitch searching algorithm that was implimented in the QCELP vocoder. The algorithms used in this study is to reduce the pitch searching time 1) using the skip table, 2) using the symmetrical property of the autocorrelation , and 3) using the preprocessing autocorrelation, 4) using the positive autocorrelation, 5) using the preliminary pitch. Performance scores are presented for each of the five pitch searching algorithms based on computation speed and on pitch prediction error.

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Analysis on magnetizing characteristics of current limiting reactor using HTSC module

  • Han, Tae Hee;Lim, Sung Hun
    • Progress in Superconductivity and Cryogenics
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    • v.20 no.1
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    • pp.15-18
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    • 2018
  • In this paper, the magnetizing characteristics of the current limiting reactor (CLR) using $high-T_C$ superconducting (HTSC) module were analyzed. Since the saturation of iron core comprising the CLR using HTSC module deteriorates its current limiting operation, the design of the CLR using HTSC module considering the magnetizing characteristics is needed. For the analysis on the magnetizing characteristics, the flux linkage and the magnetizing current of this CLR using HTSC module were derived from its electrical equivalent circuit. Through the analysis on the linkage flux versus the magnetizing current, obtained from the short-circuit tests, the suppressing effect of the iron core's saturation was discussed.

OPTIMAL DESIGN AND FABRICATION OF SPIRAL INDUCTOR ON SILICON SUBSTRATE (실리콘 기판상에서 나선형 인덕터의 최적설계 및 제작)

  • 서종삼;박종욱이성희김영석
    • Proceedings of the IEEK Conference
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    • 1998.10a
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    • pp.645-648
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    • 1998
  • We used a three-dimensional inductance extraction program, Fasthenry for optimal design of the spiral inductors on silicon substrate. The inductance and quality factor of the spiral inductors with various design parameters were calculated so that the optimal parameter value was determined. The spiral inductors then were fabricated using different foundary processes and were measured using the network analyzer and microwave probes. The pad and other parasitics of measurement system were de-embedded using the y-parameter calibration technique. the inductors fabricated using the LG 0.8um process and HP 0.5um process showed the quality factor of 5.8 and 3, respectively. Finally the equivalent circuit farameters of the spiral inductors on silicon substrate were extracted from the measurement data using the matlab.

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Context-free multiple-object segmentation using attention operator based on modified generalized symmetry transform (일반화 대칭변환을 변형한 관심 연산자에 의한 사전 정보없는 다중 물체 분할)

  • 구태모;전준형;최흥문
    • Journal of the Korean Institute of Telematics and Electronics C
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    • v.34C no.4
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    • pp.36-44
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    • 1997
  • An efficient context-free multiple-object segmentation using attention operator based on modified generalized symmetry transform is proposed and implemented by modifying a radial basis function network. By using the difference of intensity gradient, instead of te intensity gradient itself, in generalized symmetry tranform so as to make the attention operator to preserve the edges of the objects shape, an efficient context-free multiple-object segementation is proposed in which no a priori shape informtion on the objects is requried. The attention operator is implemented by using a modified radial basis function network which can reflect symmetry, and by using te edge pyramid of the input image, both of the local and the global symmetry of the objects are reflected simultaneously to make the multiple-object with different sizes be segmented with a singel fixed-size $n\timesm$ can be done with O(n) complexity. The simulaton results show that the proposed algorithm can efficiently be used in context-free multiple-object segmentation even for the low contrast IR images as well as for the images from the camera.

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An Experimental Study on Carbonation Resistance of Concrete Depending on Surface Treatment of Lightweight Aggregates (경량골재의 표면처리에 따른 콘크리트의 탄산화 저항성에 관한 실험적 연구)

  • Eom, In-Hyeok;On, Jea-Hoon;Kim, Young-Soo
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2012.11a
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    • pp.89-91
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    • 2012
  • The purpose of this study is to investigate the mechanical property and carbonation resistance of concretes using surface treated lightweight aggregate. In order to evaluate mechanical property and carbonation resistance, slump, compressive strength, and carbonation depth are tested. Slump of concretes using surface treated lightweight aggregate measured 120~125mm, which are lower than slump of NWAC. Compared to compressive strength of NWAC, compressive strength of concretes using surface treated lightweight aggregate showed a level of 82.8~95.9%. In carbonation resistance test, carbonation depth of concretes using surface treated lightweight aggregate measured 10.2~11.3mm, which are lower than carbonation depth of NWAC. As a result, it is found that compressive strength is decreased slightly but carbonation resistance is improved, in case of using surface treated lightweight aggregate.

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A fundamental Study on the properties of Concrete by using the Rapid Hardening Blast Furnace Slag Cement (조강슬래그시멘트를 이용한 콘크리트의 기초물성에 관한 연구)

  • 김진춘;최광일
    • Proceedings of the Korea Concrete Institute Conference
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    • 1995.04a
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    • pp.72-77
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    • 1995
  • Blast-furnace slag cement has been used widely as a structural material due to the latent hydraulicity of granulated ground blast furnace slag(GGBS)for a long time as The wall as ordinary portland cement. In this study, based on the fundamental investigation on the high strength and high durable concrete using the high fineness GGBS the following remarks can be made. 1) The average desired strenth of concrete is Or=600~800kg/$\textrm{cm}^2$. 2) The above high strength concrete using the high fineness GGBS is more workable than those using only OPC. 3) The adiabatic temperature and drying shringkage decrease, so the density and resistance to sea water attack increase as results. 4)The unit cement content and unit air entrained admixture at the same desired strength of concrete decrease, so the economical high strength concrete can be manufactured from using the high fineness GGBS.

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A Study on the Error Associated with Ventilation Rate Calculation Using Different Sampling Intervals (측정시간에 따른 거주주택의 환기량 계산 오류에 관한 연구)

  • 양원호;배현주;이기영;정문호
    • Journal of Environmental Health Sciences
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    • v.26 no.3
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    • pp.50-54
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    • 2000
  • Ventilation rates can be measured directly by a tracer decay method, although little is known of the effects of different sampling intervals on decay rte calculations. This study determined variations in decay rates calculated by three techniques using residential ozone decay data. The calculation techniques were a regression technique, decay techniques using half-life and average-life, and finite difference techniques using two different time intervals. Variation associated with regression technique calculations for residential ozone decay rates based on data from both sample intervals were within 10% (2.81$\pm$1.88 hr-1). However, both half-life and finite difference technique calculations using a shorter-time interval were significantly different from those obtained with the regression technique(p<0.05). Therefore, the use of short sampling intervals in tracer decay may cause significant error in decay rate calculations.

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Low Temperature Debinding Process Using Oxygen Plasma for Flexible Printed Electronics

  • Lee, Young-In
    • Journal of Powder Materials
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    • v.19 no.5
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    • pp.343-347
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    • 2012
  • In this study, an oxygen plasma treatment was used as a low temperature debinding method to form a conductive copper feature on a flexible substrate using a direct printing process. To demonstrate this concept, conductive copper patterns were formed on polyimide films using a copper nanoparticle-based paste with polymeric binders and dispersing agents and a screen printing method. Thermal and oxygen plasma treatments were utilized to remove the polymeric vehicle before a sintering of copper nanoparticles. The effect of the debinding methods on the phase, microstructure and electrical conductivity of the screen-printed patterns was systematically investigated by FE-SEM, TGA, XRD and four-point probe analysis. The patterns formed using oxygen plasma debinding showed the well-developed microstructure and the superior electrical conductivity compared with those of using thermal debinding.