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Development of Magnetically Separable Immobilized Lipase by Using Cellulose Derivatives and Their Application in Enantioselective Esterification of Ibuprofen  

Lee, Go-Woun (Department of Chemical Engineering, Chosun University)
Joo, Hong-Il (Department of Chemical Engineering, Chosun University)
Kim, Jung-Bae (Department of Chemical and Biological Engineering, Korea University)
Lee, Jung-Heon (Department of Chemical Engineering, Chosun University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.3, 2008 , pp. 465-471 More about this Journal
Abstract
Highly active, stable, and magnetically separable immobilized enzymes were developed using carboxymethyl cellulose (CMC) and diethylaminoethyl cellulose DEAE-C; hereafter designated "DEAE" as supporting materials. Iron oxide nanoparticles penetrated the micropores of the supporting materials, rendering them magnetically separable. Lipase (LP) was immobilized on the surface of the supporting materials by using cross-linked enzyme aggregation (CLEA) by glutaraldehyde. The activity of enzyme aggregates coated on DEAE was approximately 2 times higher than that of enzyme aggregates coated on CMC. This is explained by the fact that enzyme aggregates with amine residues are more efficient than those with carboxyl residues. After a 96-h enantioselective ibuprofen esterification reaction, 6% ibuprofen propyl ester was produced from the racemic mixture of ibuprofen by using DEAE-LP, and 2.8% using CMC-LP.
Keywords
Enzyme stabilization; ibuprofen; racemic resolution;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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