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Gene Expression of Detoxification Enzymes in Tenebrio molitor after Fungicide Captan Exposure (살진균제인 캡탄 처리 후 갈색거저리의 해독효소 유전자 발현)

  • Jang, Ho am;Baek, Hyoung-Seon;Kim, Bo Bae;Kojour, Maryam Ali Mohammadie;Patnaik, Bharat Bhusan;Jo, Yong Hun;Han, Yeon Soo
    • Korean journal of applied entomology
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    • v.61 no.1
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    • pp.155-163
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    • 2022
  • The application of fungicides is indispensable to global food security, and their use has increased in recent times. Fungicides, directly or indirectly, have impacted insects, leading to genetic and molecular-level changes. Various detoxification mechanisms allow insects to eliminate reactive oxygen species (ROS) toxicity induced by agrochemicals including fungicides. In the present study, we analyzed the mRNA expression levels of detoxifying enzymes in Tenebrio molitor larvae following exposure to non-lethal doses (0.2, 2, and 20 ㎍/µL) of a fungicide captan. Transcripts of peroxidases (POXs), catalases (CATs), superoxide dismutases (SODs), and glutathione-s-transferases (GSTs) were screened from the T. molitor transcriptome database. RT-qPCR analysis showed that TmPOX5 mRNA increased significantly 24 h post-captan exposure. A similar increase was noticed for TmSOD4 mRNA 3 h post-captan exposure. Moreover, the expression of TmCAT2 mRNA increased significantly 24 h post-treatment with 2 ㎍/µL captan. TmGST1 and TmGST3 mRNA expression also increased noticeably after captan exposure. Taken together, these results suggest that TmPOX5 and TmSOD4 mRNA can be used as biomarkers or xenobiotics sensors for captan exposure in T. molitor, while other detoxifying enzymes showed differential expression.

GMI Microwave Sea Surface Temperature Validation and Environmental Factors in the Seas around Korean Peninsula (한반도 주변해 GMI 마이크로파 해수면온도 검증과 환경적 요인)

  • Kim, Hee-Young;Park, Kyung-Ae;Kwak, Byeong-Dae;Joo, Hui-Tae;Lee, Joon-Soo
    • Journal of the Korean earth science society
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    • v.43 no.5
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    • pp.604-617
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    • 2022
  • Sea surface temperature (SST) is a key variable that can be used to understand ocean-atmosphere phenomena and predict climate change. Satellite microwave remote sensing enables the measurement of SST despite the presence of clouds and precipitation in the sensor path. Therefore, considering the high utilization of microwave SST, it is necessary to continuously verify its accuracy and analyze its error characteristics. In this study, the validation of the microwave global precision measurement (GPM)/GPM microwave imager (GMI) SST around the Northwest Pacific and Korean Peninsula was conducted using surface drifter temperature data for approximately eight years from March 2014 to December 2021. The GMI SST showed a bias of 0.09K and an average root mean square error of 0.97K compared to the actual SST, which was slightly higher than that observed in previous studies. In addition, the error characteristics of the GMI SST were related to environmental factors, such as latitude, distance from the coast, sea wind, and water vapor volume. Errors tended to increase in areas close to coastal areas within 300 km of land and in high-latitude areas. In addition, relatively high errors were found in the range of weak wind speeds (<6 m s-1) during the day and strong wind speeds (>10 m s-1) at night. Atmospheric water vapor contributed to high SST differences in very low ranges of <30 mm and in very high ranges of >60 mm. These errors are consistent with those observed in previous studies, in which GMI data were less accurate at low SST and were estimated to be due to differences in land and ocean radiation, wind-induced changes in sea surface roughness, and absorption of water vapor into the microwave atmosphere. These results suggest that the characteristics of the GMI SST differences should be clarified for more extensive use of microwave satellite SST calculations in the seas around the Korean Peninsula, including a part of the Northwest Pacific.

Annual Variation on Observation and Activity Pattern of Korean Chipmunk (Tamias sibiricus) in the Seoraksan and Jirisan National Parks, South Korea (설악산과 지리산 국립공원에 서식하는 다람쥐의 연중 관찰 양상과 행동 패턴)

  • Eom, Tae-Kyung;Lee, Jae-Kang;Lee, Dong-Ho;Ko, Hyeongyu;Bae, Ho-Kyoung;Kim, Kyu-Jung;Hwang, Hyun-Su;Park, Go Eun;Choi, Won-Il;Lim, Jong-Hwan;Park, Chan-Ryul;Rhim, Shin-Jae
    • Korean Journal of Environment and Ecology
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    • v.36 no.4
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    • pp.361-367
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    • 2022
  • This study was conducted to identify annual variation of observation and activity pattern of Korean chipmunk (Tamias sibiricus) using camera traps in the Seoraksan and Jirisan National Parks, South Korea from May 2019 to May 2021. The annual variation was identified based on the observed frequency through weekly observations. Daily activity patterns of the species were also analyzed by season. The daily activity pattern of chipmunk appeared to be constantly diurnal across the years regardless of habitat or season. The Korean chipmunks living in the two different regions were observed in different time periods throughout the year. While the chipmunks inhabiting the Seoraksan were observed from 18th to 45th week, the chipmunks inhabiting the Jirisan National Park were observed from 7th to 48th week. This may be influenced by the hibernation period of chipmunks in the two different regions. In both regions, chipmunks were most frequently observed in autumn. It is considered that seasonal variation on population dynamic and activity patterns of chipmunks were reflected in the observation frequency. Although the observation frequency of camera trap is an indirect indicator and thus having a limitation that it cannot distinguish the population density and amount of activity for the target species, camera trapping is still an effective survey technique for monitoring mammals due to its high accessibility and easy use.

Growing Environment Characteristics and Vegetational Structure of Sageretia thea, Medicinal Plant (약용식물 상동나무 자생지 생육환경 특성과 식생구조)

  • Son, Yonghwan;Son, Ho Jun;Park, Gwang Hun;Lee, Dong Hwan;Cho, Hyejung;Lee, Sun-Young;Kim, Hyun-Jun
    • Korean Journal of Plant Resources
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    • v.35 no.5
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    • pp.594-606
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    • 2022
  • This study was conducted to figure out the environment factors including vegetation structure and soil characteristics in natural habitats of Sageretia thea, and offers the basic information for habitats conservation and proliferation. The natural habitats of Sageretia thea were located at altitudes between 0~370 m with inclinations ranged as 3~35°. Through the vegetation research, the dominant species of tree layers were found to be divided into four communities. Cornus macrophylla (Com. I), Pinus thunbergii - Cinnamomum camphora (Com. II), Machilus thunbergii (Com. III), and Pinus thunbergii (Com. IV). The Species diversity (H') was 1.397~1.455, evenness (J') was 0.972~0.986, and dominance (D) was found to be 0.014~0.028. As a result of the physicochemical characteristics of soils, habitats soil mainly consisted of sandy soil and sandy loam soil. The average soil pH was 5.28~5.98, electronic conductivity was 0.22~63 ds/m, soil organic matter was 13.33~19.33 cmol+/kg, Exchange cations were appeared in the order of Ca2+, Mg2+, K+, and Na+. The Ordination result showed that Correlation coefficient between communities and environmental factors were significantly correlated with 4 main factors altitude, electronic conductivity, cation exchange capacity, exchangeable Na+. As expected, The result of this study will be helpful information on the preservation and mass production for use.

The Research Trend and Narrative Expandability of Borderlands Studies in Europe and North America -A Review Article: Globalizing Borderlands Studies in Europe and North America (유럽과 북미에서의 접경지대 연구 동향과 서사의 확장성 -『유럽과 북미 지역 접경지대 연구의 세계화』 읽기)

  • Ban, Kee-Hyun
    • Journal of Popular Narrative
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    • v.26 no.2
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    • pp.251-276
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    • 2020
  • The purpose of this article is to critically read Globalizing Borderlands Studies in Europe and North America to examine trends in border studies conducted so far in Europe and North America and to discuss the expandability and limitations of the narrative. It introduces a variety of case studies covering the borderlands of Europe and North America from ancient to modern times. It consists of a total of 10 chapters, in addition to the introduction chapter to clarify the purpose and definition of the collaboration and the short conclusion chapter on the prospects for the future of borderlands studies. This volume has some important implications for current borderland research in two main respects. First, it can introduce us we the areas and targets that the leading researchers from European and North American academia (usually the United States') have paid attention to. It also examines the current status of borderland research and predicts whether it will be possible to study various border areas where exist in other regions (especially in Asia) based on accumulating academic achievements, as well as the possibility of expansion of so-called 'globalization'. Second, it introduces the borderland as a conceptual space, beyond the border area as a physical space that is commonly thought of when it comes to 'border'. Cases of "conceptual borderlands" can be applied to a number of topics ranging from an individual's identities to the methods of governance, religions, economies, social institutions, families, labor issues, public health services and gender issues. There are, however, also some questions to be noted in the volume: the lack of consistent use of terminology, which can be considered general problems of collaboration studies; the fact that the authors still tend to understand borderlands within the imperialist discourse, perhaps because of their academic background is situated mainly in Europe and North America; the borderlands cases described here as the areas of conflict and struggle only. Nevertheless, the book is of significance in that it suggests a possibility of various borderlands studies and helps us to have better understanding of the current geopolitical situation imposed on the Korean Peninsula, which is located on the borderland between the continental and maritime powers.

Future Prospects of Forest Type Change Determined from National Forest Inventory Time-series Data (시계열 국가산림자원조사 자료를 이용한 전국 산림의 임상 변화 특성 분석과 미래 전망)

  • Eun-Sook, Kim;Byung-Heon, Jung;Jae-Soo, Bae;Jong-Hwan, Lim
    • Journal of Korean Society of Forest Science
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    • v.111 no.4
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    • pp.461-472
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    • 2022
  • Natural and anthropogenic factors cause forest types to continuously change. Since the ratio of forest area by forest type is important information for identifying the characteristics of national forest resources, an accurate understanding of the prospect of forest type change is required. The study aim was to use National Forest Inventory (NFI) time-series data to understand the characteristics of forest type change and to estimate future prospects of nationwide forest type change. We used forest type change information from the fifth and seventh NFI datasets, climate, topography, forest stand, and disturbance variables related to forest type change to analyze trends and characteristics of forest type change. The results showed that the forests in Korea are changing in the direction of decreasing coniferous forests and increasing mixed and broadleaf forests. The forest sites that were changing from coniferous to mixed forests or from mixed to broadleaf forests were mainly located in wet topographic environments and climatic conditions. The forest type changes occurred more frequently in sites with high disturbance potential (high temperature, young or sparse forest stands, and non-forest areas). We used a climate change scenario (RCP 8.5) to establish a forest type change model (SVM) to predict future changes. During the 40-year period from 2015 to 2055, the SVM predicted that coniferous forests will decrease from 38.1% to 28.5%, broadleaf forests will increase from 34.2% to 38.8%, and mixed forests will increase from 27.7% to 32.7%. These results can be used as basic data for establishing future forest management strategies.

Effects of Shading Rate, Rooting Media and Plant Growth Regulators on Rooting of Veronoca L. by Cuttings (삽목 번식 시 차광정도, 삽목용토 및 생장조절제 처리가 꼬리풀(Veronica L.)의 발근에 미치는 영향)

  • Cheon Young, Song;Ja Young, Moon;Jung Won, Sung;Byeong Seon, Park;Jae Ik, Nam;Jeong Min, Kim
    • Journal of Practical Agriculture & Fisheries Research
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    • v.24 no.4
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    • pp.35-44
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    • 2022
  • This study was conducted to develop a mass production for a commercial use by cutting of 4 kinds of V. glabrifolia Kitag., V. pusanensis Y.N.Lee, V. glabrifolia Kitag. × V. Spicata 'Alba' and V. spicata 'Ulster Blue Dwarf' × V. longifolia. Veronica L. Effects of shade, media and concentrations of plant growth regulators on the rooting of Veronica L. were examined. The rooting rate of V. glabrifolia Kitag. was higher as a 60% than the V. pusanensis Y.N.Lee as a 20% in the control of commercial media. In shading treatment, the rooting rate was highest at 30% or 60% shading, and the 30% shading was the best in number of root and root length, but the 90% shading was lower than no shading treatment. For cutting media, the rock wool and 100% perlite increased the rooting rate by more than 10% compared to commercial media, and increased the number of roots by 2 or 3 times. However the Cocopeat of media was lower of rooting rate, root number, and root length compared to another treatment. In the plant growth regulator treatment, the rate of rooting increased in all treatment compared to control, and was highest at IBA 1,000mg·L-1 as a 82.2% and NAA 200 or 400mg·L-1 as a 82.2% or 84.4% respectively, in the V. glabrifolia Kitag. × V. Spicata 'Alba'. However, the root number and root length was decreased as the concentration of growth regulators increased.

Fertilizer Effect of Waste Nutrient Solution in Greenhouses for Young Radish Cultivation (열무 재배를 위한 시설하우스 폐양액의 비료 효과)

  • Hong, Youngsin;Moon, Jongpil;Park, Minjung;Son, Jinkwan;Yun, Sungwook
    • Journal of Bio-Environment Control
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    • v.31 no.4
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    • pp.460-467
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    • 2022
  • The purpose of this study is to enhance utilization of the waste nutrient solution (WNS) disposed at the hydroponic greenhouse. Several sets of testing were conducted to examine the effects of WNS: (a) a fertilizer effect, (b) soil column leaching, and (c) crop cultivation. The fertilizer effect test was applied in young radish cultivation by examining the growth characteristics of young radish and soil based on inorganic nitrogen according to the soil treatment of the nitrogen fertilizer (NF) and the WNS. The fertilizer effects and crop cultivation test were conducted with five treatments (A-E): A, non-treatment (water); B, 100% of NF; C, 70% of NF + 30% of WNS; D, 50% of NF + 50% of WNS; and E, 30% of NF + 70% of WNS. The soil column leaching test was conducted with three treatments: non-treatment (water), 100% of NF, 50% of WNS + 50% of NF. As a result, the chemical properties of the WNS were pH 6.0, EC 2.4dS·m-1, total phosphorus (T-P) 28mg·L-1, ammonium nitrogen (NH4-N) 5.0mg·L-1, and nitrate nitrogen (NO3-N) 301mg·L-1. The chemical properties of the soil were pH 5.51, EC 0.31dS/m, organic matter 2.08g·kg-1, NO3-N 9.64mg·kg-1, and NH4-N 3.20mg·kg-1. The results of fertilizer effects showed that the ratio of 50% or less of NF and 50% or more of WNS was high in young radish growth. There was no statistically significant difference between the soil chemistry in the C-E treatments where WNS was mixed with NF and the B treatment where only NF was applied. As a result of the soil column leaching test, there was no significant difference in the concentrations of NO3 and NH4 in the treatment of 100% of NF and 50% of NF + 50% of WNS. The study indicates, if the mixed fertilizer of WNS and NF is applied in the soil cultivation of young radish, it will reduce the use of NF and environmental pollution. This also helps reduce production costs on farmers and increase the yield of young radish.

Changes of nutritional constituents and antioxidant activities by the growth periods of produced ginseng sprouts in plant factory (식물공장에서 생산된 새싹인삼의 생육 시기에 따른 영양성분 및 항산화 활성 변화)

  • Seong, Jin A;Lee, Hee Yul;Kim, Su Cheol;Cho, Du Yong;Jung, Jea Gack;Kim, Min Ju;Lee, Ae Ryeon;Jeong, Jong Bin;Son, Ki-Ho;Cho, Kye Man
    • Journal of Applied Biological Chemistry
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    • v.65 no.3
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    • pp.129-142
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    • 2022
  • Ginseng sprouts, which can be eaten from leaves to roots, has the advantage of not having to use pesticides without being affected by the season by using smart farms. The optimal cultivation timing of sprout ginseng was checked and the nutritional content and antioxidant activity were compared and analyzed. The values of total fatty acids and total minerals were no significant changes during the growth periods. The contents of total amino acids were slightly decreased to 45 days and after increased to 65 days. When the growth period was 65 days, arginine had the highest content of 3309.11 mg/100 g. The total phenolic contents were high at 3.73 GAE mg/g on the 45 days, and the total flavonoid contents were also the highest at 9.04 RE mg/g on the 45 days. The contents of total ginsenoside was not noticeable for the growth periods (29.83 on 25 days→32.77 on 45 days→26.02 mg/g on 65 days). The ginsenoside Rg2 (0.62 mg/g), Re (8.69 mg/g), Rb1 (4.75 mg/g) and Rd (3.47 mg/g) had highest contents on 45 days during growth. The values of phenolic acids and flavonols were gradually increased to 45 days (338.6 and 1277.14 ㎍/g) and then decreased to 65 days. The major compounds of phenolic acids and flavonols were confirmed to benzoic acid (99.03-142.33 ㎍/g) and epigallocatechin (416.03-554.64 ㎍/g), respectively. The values of 2,2-diphenyl-1-picrylhydrazyl (44.27%), 2,4,6-azino-bis (3-ethylbenzothiazoline-6-sulphnoic acid) diammonium salt (75.16%), and hydroxyl (63.29%) radical scavenging activities and ferric reducing/antioxidant power (1.573) showed the highest activity on the 45 days as well as results of total phenolic and total flavonoid contents.

Development and Validation of the Korean Implementation Fidelity Checklist of Tier 1 School-Wide Positive Behavior Support (KIFC-T1) (한국형 학교차원 긍정적 행동지원 1차 실행충실도 척도(KIFC-T1)의 개발과 타당화)

  • Nam, Dong Mi;Chang, Eun Jin;Won, Sung-Doo;Cho Blair, Kwang-Sun;Song, Wonyoung
    • Korean Journal of School Psychology
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    • v.17 no.3
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    • pp.401-419
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    • 2020
  • The purpose of this study was to validate the Korean Implementation Fidelity Checklist of Tier 1 School-Wide Positive Behavior Support (KIFC-T1) for use in the Korean educational system. Tier 1 support, which is universal supports, within a multi-tiered, school-wide positive behavior support (SWPBS) model, aims to provide support to and prevent problem behaviors among all students in a school. The initial KIFC-T1 consisted of 48 items and 11 factors and was developed based on a literature review. Its content was validated by experts. The validated KIFC-T1 was introduced to 185 special school teachers who had experience implementing SWPBS and who used the instrument to assess the degree to which their schools had implemented Tier 1 support. Based on their responses, the construct validity of the KIFC-T1 was examined using factor, item, and internal consistency reliability analyses. The concurrent validity of the tool was examined using the PBS Evaluation Tool, School Climate Questionnaire, School Discipline Practice Scale, and PBS Effectiveness Scale. The analyses revealed that KIFC-T1 had a stable five-factor structure with 35 items, had good reliability (Cronbach's α=.956, each factor's Cronbach's α=.834-.951), and its results were statistically significantly correlated with those of the PBS Evaluation Tool, School Discipline Practice Scale, and the PBS Effectiveness Scale. However the KIFC-T1's results were not statistically significantly correlated with the results of the School Climate Questionnaire. These results suggest that KIFC-T1 is a reliable and valid tool for assessing the fidelity of universal support implementations.