• 대한약침학회지
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• 제5권2호
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• pp.6-24
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• 2002

This experiment was carried out to study on the safety assessment of Bos tures$^{\circ}{\S}Ursus thibetanus^{\circ}{\S}$Moschus extract solution(BUM) for Herbal-acupuncture. SD rats and ICR mice were used for acute toxicity test. the results were summerized as follows; 1. In rats and mice, LD50 value could not be measured. 2. There were no abnormal finding in acute toxicity test treated BUM for Herbal-acupuncture

• 대한약침학회지
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• 제4권3호
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• pp.69-83
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• 2001

This experiment was carried out to study on the safety assessment of Bas tures . Ursus thibetanus extract solution(BU) for Herbal acupuncture. SD rats and ICR mice were used for acute toxicity test the results were summerized as follows; 1. In rats and mice, LD50 value could not be measured. 2. There were no abnormal finding in acute toxicity test treated BU for Herbal-acupuncture.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제4권4호
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• pp.154-158
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• 2023

The Asiatic black bear, Ursus thibetanus, is among the most threatened or endangered species in Asia. For its conservation and management, sex identification of U. thibetanus using non-invasive samples (e.g., hair and/or feces) is potentially valuable. In this study, a non-invasive molecular method for sex identification of U. thibetanus samples collected from various countries was first utilized, and it was based on polymerase chain reaction (PCR) amplification of the amelogenin gene via PCRs. Thirty-three bear DNA samples, extracted not only from blood (n=9) but also from hair (n=18) and feces (n=6), were used. We performed sex-specific PCR amplifications of the amelogenin gene using a primer set, SE47 and SE48. The primer set could successfully amplify a single X-specific band for females and both X- and Y-specific bands for males from all blood (100%) and hair (100%) samples. In addition, the primer set could distinguish the sex of bears in four out of a total of six fecal samples (approximately 67%). This study's findings suggest that this molecular method can be applied to sex identification of Asiatic black bears from various Asian regions using non-invasive samples, such as hair and feces.

• 한국임상수의학회지
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• 제32권4호
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• pp.363-365
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• 2015

반달가슴곰 (Ursus thibetanus)은 국제적 멸종위기종으로, 국립공원관리공단에서 종복원 프로그램을 진행 중이다. 하지만 자연방사가 어려운 개체들은 제한된 사육 시설로 인해 곰 개체수 관리가 필요하여 수컷에서 고환절제술을 시행하였다. Tiletamine-zolazepam 2 mg/kg과 medetomidine $40{\mu}g/kg$ 합제를 이용하여 곰을 포획하고, 기관 삽관을 통해 isoflurane으로 전신마취를 유지하였다. 고환절제술은 전음낭 절개를 통해 closed method로 시행되었으며, LigaSure를 사용하여 정삭을 소락 절단였다. 절개면 피하조직은 흡수성 봉합사로 연속봉합 하였으며, 피부는 조직접착제로 봉합하였다. 별다른 부작용 없이 전신마취에서 회복되었으며, 총 마취 시간은 58분(곰 A)과 53분(곰 B)이었다. 수술시간은 26분(곰 A)과 24분(곰 B)이었으며, 술 후 부종이나 합병증은 관찰되지 않았다. 본 연구는 반달가슴곰에서 LigaSure를 이용한 최초의 고환절제술 증례이다.

• 한국환경생태학회:학술대회논문집
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• 한국환경생태학회 2007년도 임시총회 및 학술논문발표회
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• pp.101-103
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• 2007

• 한국환경생태학회:학술대회논문집
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• 한국환경생태학회 2008년도 추계 학술대회
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• pp.126-128
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• 2008

• 한국한의학연구원논문집
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• 제3권1호
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• pp.153-167
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• 1997

Conventionally, identification and classification methods of natural products include the morphological survey and assay of chemical disposition, sing these methods, however, is not satisfying for the precise identification of natural products because they are often valiable in the compositions and morphology To standardize the natural products identification and classification, genomic DNA analysis such as RAPD, RFLP and Amp-FLP can be adopted for this purpose. In this study, various ginsengs and bear gall bladder were tested for the development of genetic identification and classification method. Varieties of ginsengs such as, P. ginseng, P. quinquefolium, P. japonicus and P. notoginseng, were genetically analyzed by RAPD. Also, DNA isolated from Bear blood and gall bladder, Ursus thibetanus, Ursus americanus and Ursus arctos, were analyzed by the same method. The results demonstrated that the identification and classification of bear gall bladder and various ginsengs were possible by RAPD analysis. Therefore, this method was thought to be used as a additional method for the identification and classification of other natural products.

• 한국환경생태학회:학술대회논문집
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• 한국환경생태학회 2008년도 추계 학술대회
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• pp.123-125
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• 2008

• 환경생물
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• 제25권3호
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• pp.215-222
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• 2007

비무장지대(DMZ)를 포함한 민통선 일대는 지난 50여 년간 사람에 의한 인위적인 간섭을 적게 받은 지역으로 자연생태계가 잘 보전된 세계적인 환경보전지역이다. 그러나 최근 들어 개발에 따른 급격한 환경파괴가 가속화되고 있어 이에 대한 대비가 시급한 실정이다. 현재까지 발표된 조사지역에 대한 논문, 보고서 등 문헌자료와 청문조사를 토대로 조사된 포유동물상을 파악하였다. DMZ 및 군사접경지역 전체에서 서식하는 포유동물 중 문헌상으로 서식이 확인된 종은 모두 6목 17과 51종으로 조사되었으며, 이중 화천군, 철원군, 양구군 일대 중부지역에는 총 6목 14과 32종이 서식하는 것으로 조사되었다. 천연기념물로 지정된 종은 반달가슴곰(Ursus thibetanus), 수달(Lutra lutra), 사향노루(Moschus moschiferus), 산양(Nemorhaedus caudatus), 하늘다람쥐(Pteromys volans), 물범(Phoca largha)등 3목 6과 6종이었다. 이 조사를 통해 얻어진 종합적인 자연문화재 기초자원 조사결과는 향후, UNESCO의 세계자연보전지역 등록에 대비한 기초자료로 활용될 수 있을 것으로 판단된다.

• 한국임상수의학회지
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• 제40권6호
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• pp.438-444
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• 2023

The aim of this study was to analyze the learning curve of a low-volume veterinary surgeon for laparoscopic salpingectomy of Asiatic black bear. A total of 66 Asiatic black bears (Ursus thibetanus) were presented for sterilization using laparoscopic salpingectomy. These 66 bears were divided into three groups of 22 bears according to the order of surgery (A, B, and C groups, 22 bears per group). One veterinarian performed laparoscopic salpingectomy. There was no significant difference in age, body weight, or crown-rump length between groups. The hazard of completion for salpingectomy by 5 minutes was higher (p < 0.0001) in B and C groups than A group, whereas the hazard was not significant different between B and C groups. The factor that affected the hazard of completing salpingectomy by 5 minutes was heart rate, not age or weight. Operation time was 12.61 ± 8.04 minutes for group A, 5.35 ± 4.38 minutes for group B, and 2.80 ± 1.16 minutes for group C. The operation time for salpingectomy shortened over time. However, significant difference in operation time was present only between groups A and C (p = 0.001). The operation time for laparoscopic salpingectomy decreased rapidly for the first 7 bears (p < 0.05). It then decreased gradually until the 66th case. Operation time of laparoscopic salpingectomy was significantly decreased and stabilized after 33 cases (p < 0.05). As a result, the number of bears required for a low-volume veterinary surgeon to be proficient in laparoscopic salpingectomy is 33 or more.