• Title/Summary/Keyword: Unrecorded wild yeast

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Report of two unrecorded yeast species in the class Tremellomycetes

  • Seonjae Kim;Sathiyaraj Srinivasan
    • Journal of Species Research
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    • v.13 no.2
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    • pp.136-141
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    • 2024
  • The purpose of this study is to isolate and identify wild yeasts from the soil samples collected in Daegu and Daejeon City, Republic of Korea. Among 15 strains isolated in this study, 13 strains were previously reported and two strains had not been reported in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation tests were done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. The two unrecorded yeast strains, PG2-2-10C and DJ2-14-10C, belong to the genus Holtermanniella (family Holtermanniaceae, order Holtermanniales, class Tremellomycetes) and Goffeauzyma (family Filobasidiaceae, order Filobasidiales, class Tremellomycetes), respectively. The two unrecorded yeast strains had oval shape and polar budding cells. This research describers the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

Isolation and characterization of two unrecorded yeast species in the phylum Basidiomycota

  • Jieun Seok;Jaewoo Bai;Sathiyaraj Srinivasan
    • Journal of Species Research
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    • v.13 no.1
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    • pp.105-110
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    • 2024
  • The purpose of this study was to isolate and identify wild yeasts from soil samples collected in Seoul and Daejeon, Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and carbon source assimilation test were done using API 20C AUX kit. Among the 13 isolated strains, 11 strains were previously reported, but two strains have never been reported from Republic of Korea. The 13 strains were assigned to the phylum Basidiomycota. The two unrecorded yeast strains B2UV-201 and DJ1-5-B-10C belong to the genera Rhodotorula and Rhodosporidiobolus, respectively. The two unrecorded yeast strains are oval shaped and polar budding cells. This research focuses on the morphological and biochemical properties of the two unreported yeast species that have not officially been reported in Korea.

Description of unrecorded wild yeasts from soil in Republic of Korea under cold conditions

  • Soohyun Maeng;Sathiyaraj Srinivasan
    • Journal of Species Research
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    • v.13 no.2
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    • pp.142-146
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    • 2024
  • The purpose of this study was to isolate and identify wild yeasts from soil collected in Daegu City and Cheongyang County, Republic of Korea. Among 11 strains isolated in this study, nine strains were previously reported and two strains were unreported in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation test are done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. Of the two unrecorded yeast strains, CY-9-10C belongs to the genus Mrakia (family Mrakiaceae, order Cystofilobasidiales, class Tremellomycetes) and PG3-4-10C belongs to the genus Slooffia (family Chrysozymaceae, order Microbotryomycetes incertae sedis, class Microbotryomycetes). Both strains had oval-shaped and polar budding cells. This research described the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

Isolation and characterization of two unrecorded yeast species in the order Filobasidiales

  • Inyoung Choi;Sathiyaraj Srinivasan
    • Journal of Species Research
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    • v.13 no.1
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    • pp.100-104
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    • 2024
  • The purpose of this study was to isolate and identify wild yeasts from soil samples collected in Daegu and Cheongju city, Republic of Korea. To identify the wild yeast strains, pairwise sequence comparisons of D1/D2 region of the 26S rRNA gene sequence were done using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation test are done using API 20C AUX kit. All strains were assigned to the phylum Basidiomycota. Among 13 strains, 11 strains were previously reported, but two strains were unreported from the Republic of Korea. The two unrecorded yeast strains, GW1-3 and PG1-1-10C, belong to the genus Solicoccozyma (family Piskurozymaceae, order Filobasidiales, class Tremellomycetes). The two strains had oval-shaped and polar budding cells. This research showed the morphological and biochemical properties of the two unreported yeast species that had not officially reported in Korea.

Separation of Wild Yeast from Intestine of Othius punctulatus and Microbiological Characteristics of Unrecorded Wild Yeast Strains in Korea (반날개과 곤충 장으로부터 야생 효모의 분리 및 국내 미기록 효모들의 균학적 특성)

  • Lee, Sang Eun;Han, Joo Hyun;Kim, Myung Kyum
    • The Korean Journal of Mycology
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    • v.48 no.3
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    • pp.229-235
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    • 2020
  • The goal of this study was to isolate and identify the wild yeast strains in intestine of Othius punctulatus collected in Cheongju-si, Chungcheongbuk-do, Korea and characterize their unrecored yeast strains. We isolated 16 wild yeast strains from 5 intestine samples from Othius punctulatus. The 16 wild yeast strains were included three strains in the genus Aureobasidium, four strains in the genus Cystofilobasidium, one strain in the genus Mrakia, three strains in the genus Naganishia, two strains in the genus Saitozyma, two strains in the genus Sampaiozyma and one strain in the genus Scheffersomyces. Among them Cystofilobasidium capitatum YP53, Cystofilobasidium capitatum YP71, Cystofilobasidium macerans YS620, Cystofilobasidium macerans YS622, Mrakia aquatica YP158 and Scheffersomyces stipitis YI5 were recorded for the first time in Korea. The microbiological characteristics and carbon assimilation of these previously unrecorded yeasts were investigated. Almost all of them were oval-shaped. And, glycerol, L-arabinose, xylitol, and inositol are not utilized.

Isolation and characterization of unrecorded yeasts species in the family Metschnikowiaceae and Bulleribasidiaceae in Korea

  • Park, Yuna;Maeng, Soohyun;Srinivasan, Sathiyaraj
    • Journal of Species Research
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    • v.9 no.3
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    • pp.198-203
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    • 2020
  • The goal of this study was to isolate and identify wild yeasts from soil samples. The 15 wild yeast strains were isolated from the soil samples collected in Pocheon city, Gyeonggi Province, Korea. Among them, four yeast stains were unrecorded, and 11 yeast stains were previously recorded in Korea. To identify wild yeasts, microbiological characteristics were observed by API 20C AUX kit. Pairwise sequence comparisons of the D1/D2 domain of the 26S rRNA were performed using Basic Local Alignment Search Tool(BLAST). Cell morphology of yeast strains was examined by phase contrast microscope. All strains were oval-shaped and polar budding and positive for assimilation of glucose, 2-keto-ᴅ-gluconate, N-acetyl-ᴅ-glucosamine, ᴅ-maltose and ᴅ-saccharose (sucrose). There is no official report that describes these four yeast species: one strain of the genus Kodamaea in the family Metschnikowiaceae and three strains of the Hannaella in the family Bulleribasidiaceae. Kodamaea ohmeri YI7, Hannaella kunmingensis YP355, Hannaella luteola YP230 and Hannaella oryzae YP366 were recorded in Korea, for the first time.

Isolation and characterization of four unrecorded wild yeasts from the soils of Republic of Korea in winter

  • Yuna Park;Soohyun Maeng;Sathiyaraj Srinivasan
    • Journal of Species Research
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    • v.12 no.3
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    • pp.197-202
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    • 2023
  • The purpose of this study was to isolate and identify wild yeasts from the soil collected in Gwangju and Pocheon City, Gyeonggi Province, Republic of Korea. Among 10 strains, six strains were already reported, but four strains were unrecorded in Republic of Korea. To identify wild yeast strains, pairwise sequence comparisons of the D1/D2 region of the 26S rRNA gene sequence were performed using Basic Local Alignment Search Tool (BLAST). The cell morphologies were observed by phase contrast microscope and assimilation tests were carried out using API 20C AUX kit. The 10 strains were assigned to the phyla Basidiomycota (8 strains) and Ascomycota (2strains). The unrecorded four yeast strains, NH33, NH19, NH20, and YP416, belong to the phylum Basidiomycota and the genera Buckleyzyma, Leucosporidium, Holtermanniales, and Mrakia, respectively. All strains had oval-shaped and polar budding cells. In this research, the morphological and biochemical properties of four unreported yeast species were characterized intensively, which were not officially reported in Korea.

Isolating and characterizing the unrecorded Wild Yeasts from Seawater and Soil in Haeundae and Mongdol Beaches on the Southern Coast of, Korea (남해안 해운대와 몽돌 해수욕장 주변환경으로부터 야생 효모의 분리 및 국내 미기록 효모들의 균학적 특성)

  • Seon-Jeong Park;Ji-Eun Jang;Jeong-Su Moon;Hyang-Burm Lee;Jong-Soo Lee
    • The Korean Journal of Mycology
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    • v.50 no.1
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    • pp.65-73
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    • 2022
  • This study aimed to isolate wild yeasts from seawaters and soils samples of the Haeundae and Mongdol beaches on the southern coast of Korea, and to characterize these unrecorded wild yeast strains. In total, 41 strains, representing 37 different species of wild yeast were isolated from 70 samples collected from the beaches. Among these, 14 strains were isolated from the alkalophilic medium of yeast extract-peptone-dextrose (YPD) medium (pH 9.0), and 27 strains were isolated concurrently on general YPD medium (pH 6.5). Among the 41 isolated wild yeast strains, Candida insectorum HUD 16-3(JSL-KSS-002) and Metschnikowia citriensis HUD 12-5(JSL-KSS-001) had not previously been recorded. We investigated the microbiological characteristics of these two unrecorded yeast strains and three other strains-, Cystobasidium lysinophilum JSC 52-2(JSL-GGU-019), Candida takata NMD 11-1(JSL-GGU-017) and Candida panamensis ASG 58M-2(JSL-GGU-018) from Jangseoncheon in Jellabuk-do and Jangtaesan in Deajeon city. All five previously unrecorded yeasts were oval and did not form spores. All strains grew well in YPD and yeast extract-malt extract media in a vitamin-free medium. Two strains, including C. insectorum HUD 16-3(JSL-KSS-002) grew well in a 15% NaCl-containing YPD medium. Three strains, including Cys. lysinophilum JSC52-2(JSL-GGU-019) assimilated lactose, and all strains assimilated starch.

Isolation and Identification of Yeasts from Wild Flowers Collected around Jangseong Lake in Jeollanam-do, Republic of Korea, and Characterization of the Unrecorded Yeast Bullera coprosmaensis

  • Han, Sang-Min;Hyun, Se-Hee;Lee, Hyang Burm;Lee, Hye Won;Kim, Ha-Kun;Lee, Jong-Soo
    • Mycobiology
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    • v.43 no.3
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    • pp.266-271
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    • 2015
  • Several types of yeasts were isolated from wild flowers around Jangseong Lake in Jeollanam-do, Republic of Korea and identified by comparing the nucleotide sequences of the PCR amplicons for the D1/D2 variable domain of the 26S ribosomal DNA using Basic Local Alignment Search Tool (BLAST) analysis. In total, 60 strains from 18 species were isolated, and Pseudozyma spp. (27 strains), which included Pseudozyma rugulosa (7 strains) and Pseudozyma aphidis (6 strains), was dominant species. Among the 60 strains, Bullera coprosmaensis JS00600 represented a newly recorded yeast strain in Korea, and its microbiological characteristics were investigated. The yeast cell has an oval-shaped morphology measuring $1.4{\times}1.7{\mu}m$ in size. Bullera coprosmaensis JS00600 is an asporous yeast that exhibits no pseudomycelium formation. It grew well in vitamin-free medium as well as in yeast extract-malt extract broth and yeast extract-peptone-dextrose (YPD) broth, and it is halotolerant growing in 10% NaCl-containing YPD broth.

Mycological Characteristics and Carbon Source Activities of Unrecorded Wild Yeast Species from Vineyard Soil in Korea (국내 포도밭 토양에서 분리된 야생 효모 미기록종들의 균학적 특성과 탄소원 활성)

  • Bong Soon Yim;Myung Kyum Kim
    • The Korean Journal of Mycology
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    • v.51 no.4
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    • pp.373-381
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    • 2023
  • This study aimed to isolate and characterize wild yeast strains from the vineyard soil in Korea. Twenty yeast strains were isolated from vineyard soil in Gimpo-si, Gyeonggi-do, Korea, where Vitis labrusca cv. Campbell Early is grown. Eighteen strains were previously recorded in Korea. The remaining two, Cyberlindnera mrakii VG-21-10C and Starmerella bacillaris GR9 were not previously recorded in Korea. The mycological characteristics of VG-21-10C and GR9 were investigated. Both were oval-shaped, convex, and smooth. However, differences were evident in colony color and carbon assimilation activities. Strain VG-21-10C is white-colored and assimilates glucose, glycerol, D-xylose, D-cellobiose, D-saccharose, and D-raffinose as carbon sources. Strain GR9 is cream-colored and assimilates glucose, D-saccharose, and D-raffinose as carbon sources.