• Asian Pacific Journal of Cancer Prevention
• /
• 제15권20호
• /
• pp.8685-8688
• /
• 2014

The objective of the present study was to investigate cloning, expression, and functions of the recombinant protein, Siva1. Siva1 gene was synthesized by RT-PCR from HCT116 cells. Plasmids were cleaved with the restriction endonuclease, BamH1/Sal1 and products were connected to pQE30, which underwent cleavage by BamH1/Sal1. The recombinant plasmid, pQE30-Siva1, was identified after digestion with restriction endonucleases followed by transformation into E. coli M15. Expression of Siva1 was induced by IPTG and identified by SDS-PAGE following purification with affinity chromatography. The results showed that size of Siva1 was 12 kDa, consistent with the molecular weight of the His-Siva1 fusion protein. Functional test demonstrated that Siva1 significantly inhibited the invasion and migration of HCT116 cells. It may thus find clinical application for control of cancers.

• 소성∙가공
• /
• 제29권5호
• /
• pp.251-256
• /
• 2020

The purpose of this study is to investigate the effect of sintering condition on the microstructure evolution and tensile properties of the Ti-6Al-4V alloy sample processed by metal injection molding (MIM) in terms of the sizes of the alpha morphology and pore found in the matrix. For this purpose, a series of MIM were conducted on this sample at various sintering temperatures ranging from 1173 to 1373 K for three hours followed by furnace cooling, observed by the scanning electron microscopy. The microstructures sintered in this study showed that, with increasing sintering temperature over beta transus temperature, the transformation of the equiaxed alpha into transformed beta was attained while the size of pores would tend to decrease. Thus, the strength remained unchanged significantly in the tension while ductility increased to some extent as sintering temperature increased. Such mechanical behavior would be explained in relation to the microstructure evolution of the Ti-6Al-4V sample via the MIM.

• 대한원격탐사학회지
• /
• 제25권3호
• /
• pp.271-285
• /
• 2009

Multi-temporal satellite imagery can be changed into a transform image that emphasizes the changed area only through the application of various change detection techniques. From the transform image, an automated change detection model calculates the optimal threshold value for classifying the changed and unchanged areas. However, the model can cause undesirable results when the histogram of the transform image is unbalanced. This is because the model uses a single threshold value in which the sign is either positive or negative and its value is constant (e.g. -1, 1), regardless of the imbalance between changed pixels. This paper proposes an advanced method that can improve accuracy by applying separate threshold values according to the increased or decreased range of the changed pixels. It applies multiple threshold values based on the cumulative producer's and user's accuracies in the automated binary change detection model, and the analyst can automatically extract more accurate optimal threshold values. Multi-temporal IKONOS satellite imagery for the Daejeon area was used to test the proposed method. A total of 16 transformation results were applied to the two study sites, and optimal threshold values were determined using accuracy assessment curves. The experiment showed that the accuracy of most transform images is improved by applying multiple threshold values. The proposed method is expected to be used in various study fields, such as detection of illegal urban building, detection of the damaged area in a disaster, etc.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제13권7호
• /
• pp.3599-3619
• /
• 2019

In human activity recognition system both static and motion information play crucial role for efficient and competitive results. Most of the existing methods are insufficient to extract video features and unable to investigate the level of contribution of both (Static and Motion) components. Our work highlights this problem and proposes Static-Motion fused features descriptor (SMFD), which intelligently leverages both static and motion features in the form of descriptor. First, static features are learned by two-stream 3D convolutional neural network. Second, trajectories are extracted by tracking key points and only those trajectories have been selected which are located in central region of the original video frame in order to to reduce irrelevant background trajectories as well computational complexity. Then, shape and motion descriptors are obtained along with key points by using SIFT flow. Next, cholesky transformation is introduced to fuse static and motion feature vectors to guarantee the equal contribution of all descriptors. Finally, Long Short-Term Memory (LSTM) network is utilized to discover long-term temporal dependencies and final prediction. To confirm the effectiveness of the proposed approach, extensive experiments have been conducted on three well-known datasets i.e. UCF101, HMDB51 and YouTube. Findings shows that the resulting recognition system is on par with state-of-the-art methods.

• 복식문화연구
• /
• 제29권4호
• /
• pp.538-553
• /
• 2021

As interest in the sustainable fashion industry continues to increase along with climate issues, it is necessary to identify research trends in sustainable fashion and seek new development directions. Therefore, this study aims to analyze research trends on sustainable fashion. For this purpose, related papers were collected from the KCI (Korean Citation Index) and Scopus, and 340 articles were used for the study. The collected data went through data transformation, data preprocessing, topic modeling analysis, core topic derivation, and visualization through a Python algorithm. A total of eight topics were obtained from the comprehensive analysis: consumer clothing consumption behavior and environment, upcycle product development, product types by environmental approach, ESG business activities, materials and material development, process-based approach, lifestyle and consumer experience, and brand strategy. Topics were related to consumption, production, and education of sustainable fashion, respectively. KCI analysis results and Scopus analysis results derived eight topics but showed differences from the comprehensive analysis results. This study provides primary data for exploring various themes of sustainable fashion. It is significant in that the data were analyzed based on probability using a research method that excluded the subjective value of the researcher. It is recommended that follow-up studies be conducted to examine social trends.

• 생명과학회지
• /
• 제21권10호
• /
• pp.1370-1376
• /
• 2011

고세균 Sulfolobus acidocaldarius의 기능유전체학 연구를 위하여 피리미딘 생합성 유전자군의 pyrEF 유전자에 근거한 피리미딘 영양요구주를 구축하였다. 원균주는 정상적인 pyrEF 존재하에서 5-fluoroorotic acid를 첨가하면 성장이 불가능하나 피리미딘 영양요구주는 성장이 가능한 원리를 활용하였다. 자외선을 이용하여 얻어진 5-FOA 첨가에 저항성을 갖는 돌연변이주를 얻었으며, 두 돌연변이주 KH1U와 KH2U는 각각 pyrE 유전자 부분의 점돌연변이와 삽입돌연변이를 갖는 돌연변이주임을 알 수 있었다. 이 두 돌연변이 균주는 5-FOA의 첨가에 의하여 이 세포를 사멸시킬 수 있는 능력이 사라짐을 확인하였다. 정상적인 pyrEF 유전자를 갖는 Sulfolobus-E. coli 플라스미드를 이용하여 보완실험을 수행한 결과 KH2U 돌연변이주는 다시 5-FOA에 대한 저항성을 잃어버렸으며, 배지내에 피리미딘의 첨가가 없어도 생존할 수 있는 능력을 보여주는 원균주와 같은 표현형으로 회귀함을 확인하였다. 이 연구는 차후 고세균 Sulfolobus acidocaldarius의 유전자 불활성화를 통한 유전학연구에 효율적인 도구로 사용되기에 유용한 연구로 생각된다.

• 청정기술
• /
• 제28권1호
• /
• pp.63-78
• /
• 2022

첨단 전자산업 폐수 처리시설에서 발생되는 유기 폐수는 고농도의 유기물질 및 20가지 이상의 유독 난분해성 물질을 포함하고 있으며, 이를 효율적으로 처리하는 것은 첨단 전자산업의 당면 과제이다. 따라서, 첨단 전자산업 유기폐수 처리시설을 CPS (Cyber physical system)상 Water digital twin으로 구축하여 COD (Chemical Oxygen Demand), TN (Total Nitrogen), TP (Total Phosphorous) 및 TMAH (Tetramethylammonium hydroxide) 등 유기 오염물질의 제거 효율 평가가 가능한 전자산업 폐수 특화 모델 개발이 필요하다. 본 연구에서는 첨단전자산업 유기폐수 제거 메커니즘에 대한 분해 미생물의 성장과 사멸의 이론적인 반응속도식에 기반한 첨단 전자산업 폐수 특화 활성슬러지 모델(Electronics industrial wastewater activated sludge model, e-ASM)을 개발하였다. 개발한 e-ASM은 전자산업 폐수처리공정에서 발생하는 유기물 산화, 질산화, 및 탈질화 과정뿐만 아니라 TMAH 등 난분해성 유기물질의 분해과정 중 발생하는 질산화미생물의 저해(Inhibition) 작용 등 복잡한 생물학적 분해 메커니즘이 모사 가능하다. 이를 활용하여 실제 전자산업 유기폐수 처리시설을 Water Digital Twin으로 구현하여 CPS (Cyber physical system) 상에서 전자산업 폐수처리장에 폐수 유입 성상에 따라 공정 모델링, 유출수 예측, 공법 선정, 설계 효율 평가 등 다양한 목적으로 활용될 수 있다.

• 한국산학기술학회논문지
• /
• 제16권4호
• /
• pp.2744-2748
• /
• 2015

본 논문에서는 전자정부 표준 프레임워크 프로젝트 내에서 MDD/MDA 기술 적용으로 인한 생산성과 품질 향상효과를 기능점수 (Function Point) 관점과 코드 인스펙션 관점에서 분석하여 제시한다. 또한 본 사례의 프로젝트에서 적용한 MDD/MDA적용 방법을 소개한다. MDD 기법의 발전과 더불어 그 효과를 검증하기 위해 다양한 연구가 진행되고 있으나, 대부분은 사례 연구를 통해 얻어지는 교훈이나, 제한된 측정 데이터로 분석된 효과를 제시하고 있다. 본 연구에서는 효율적인 개발 방법론과 전자정부 표준 프레임워크를 활용하여 MDD를 실제 정보시스템 개발 프로세스에 적용한 사례 연구를 통해 새로운 측정지표로 정량적 효과성을 측정하였다. 더불어 Application Life-Cycle 환경에서 모델링 된 정보들을 통합 저장소를 통하여 관리함으로써 추적성과 가시성이 확보된 방안을 제시한다.

• 센서학회지
• /
• 제31권6호
• /
• pp.423-427
• /
• 2022

A pressure sensor that mimics the sensing ability of human skin has emerged as high-profile technology because it shows remarkable applications in numerous fields such as robotics, human health monitoring, and artificial prosthetics. Whereas recent pressure sensors have achieved high sensitivity similar to that of human skin, they still show limited detection bandwidth. Moreover, once these e-skin are fabricated, their sensitivity and stiffness are fixed; therefore, they can be used for only limited applications. Our study proposes a new adaptive pressure sensor built with uniform gallium microdroplet-elastomer composite. Based on the phase transition of gallium microdroplets, the proposed sensor undergoes mode transformation, enabling it to have a higher sensitivity and wider detection bandwidth compared with those of human skin. In addition, we succeeded in extending a single adaptive pressure sensor to sensor arrays based on its high uniformity, reproducibility, and large-scale manufacturability. Finally, we designed an adaptive e-skin with the sensor array and demonstrated its applications on health monitoring tasks including blood pulse and body weight measurements.

• 한방안이비인후피부과학회지
• /
• 제33권3호
• /
• pp.1-26
• /
• 2020

Objectives : This study was to examine the effects of 3 types of BTS which were excluded talcum only or replaced talcum to Lonicera japonicae Flos or Kochiae Fructus on the DNCB-induced atopic dermatitis in mice. Methods : In this study, Balb/c mice were divided into five groups: normal, control, GBT(BTS except talcum), GBTG(GBT added Lonicera japonicae Flos), and GBTJ(GBT added Kochiae Fructus). And the effects on atopic dermatitis were evaluated by weight change, ear's thickness and weight, thickness of dorsal skin, severity scale of dorsal skin, histopathologic findings of dorsal skin by H&E and toluidine blue stain, proliferation of splenocyte and thymocyte in vitro, proliferation of splenocyte in vivo, IL-4, TNF-α, IgE in serum. Results : There were no significantly changes in body weight and effect of ear's weight in GBT, GBTG, and GBTJ group. The thickness of ear of GBT and GBTJ group showed significant decrease. And the thickness of dorsal skin of GBTJ group significantly decreased compared to the control, GBT, and GBTG group. All the treated groups significantly decreased in severity scale, histopathologically reduced epidermal thickness, and mast cell infiltration. In vitro, all the treated groups increased in the proliferation rates of splenocyte. However, in vivo study, it showed a falling tendency and GBT group significantly decreased compared to control, GBTG, and GBTJ group. In vitro study, GBTG group significantly decreased in the proliferation rates of thymocyte. There was no IgE contents chnage in GBT, GBTG, and GBTJ groups but IL-4 and TNF-α contents were significantly decreased. Conclusions : GBT, GBTG, and GBTJ are expected to improve symptoms of atopic dermatitis and further studies are needed for development of BTS's transformation.