• Journal of Microbiology and Biotechnology
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• 제22권6호
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• pp.780-787
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• 2012

The maize pathogen Gibberella moniliformis produces fumonisins, a group of mycotoxins associated with several disorders in animals and humans, including cancer. The current focus of our research is to understand the regulatory mechanisms involved in fumonisin biosynthesis. In this study, we employed a proteomics approach to identify novel genes involved in the fumonisin biosynthesis under nitrogen stress. The combination of genome sequence, mutant strains, EST database, microarrays, and proteomics offers an opportunity to advance our understanding of this process. We investigated the response of the G. moniliformis proteome in limited nitrogen (N0, fumonisin-inducing) and excess nitrogen (N+, fumonisin-repressing) conditions by one- and two-dimensional electrophoresis. We selected 11 differentially expressed proteins, six from limited nitrogen conditions and five from excess nitrogen conditions, and determined the sequences by peptide mass fingerprinting and MS/MS spectrophotometry. Subsequently, we identified the EST sequences corresponding to the proteins and studied their expression profiles in different culture conditions. Through the comparative analysis of gene and protein expression data, we identified three candidate genes for functional analysis and our results provided valuable clues regarding the regulatory mechanisms of fumonisin biosynthesis.

• 디지털융복합연구
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• 제11권11호
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• pp.429-434
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• 2013

2011년 정부에서는 "IT 융합 기술예측조사 2025"를 발표하였다. 본 조사보고서는 10개 ICT 산업군을 대상으로 하였다. 디지털이라는 단어와 결합된 컨버전스는 정보통신 분야의 단위기술들의 융합을 통해 등장하는 새로운 제품이나 서비스를 일컫고 있다. 이러한 컨버전스 기술의 파급효과 및 컨버전스 사회의 현상들은 이미 경제, 사회, 문화등 사회의 전 부분에서 가시화되고 있다. 본 연구에서는 10개 ICT 산업군 중에서 우리 생활과 밀접하게 연관되어 있는 4개 산업군(IT-건설, IT-자동차, IT-섬유, IT-의료)에 대한 컨버전스 관련 내용들의 필요한 기술현황 및 전망 등을 기술하였다.

• 제어로봇시스템학회논문지
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• 제21권11호
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• pp.1034-1037
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• 2015

In recent years in the field of robotics, various methods have been developed to create an intimate relationship between people and robots. These methods include speech, vision, and biometrics recognition as well as gesture-based interaction. These recognition technologies are used in various wearable devices, smartphones and other electric devices for convenience. Among these technologies, gesture recognition is the most commonly used and appropriate technology for wearable devices. Gesture recognition can be classified as contact or noncontact gesture recognition. This paper proposes contact gesture recognition with IMU and EMG sensors by using the hidden Markov model (HMM) twice. Several simple behaviors make main gestures through the one-stage HMM. It is equal to the Hidden Markov model process, which is well known for pattern recognition. Additionally, the sequence of the main gestures, which comes from the one-stage HMM, creates some higher-order gestures through the two-stage HMM. In this way, more natural and intelligent gestures can be implemented through simple gestures. This advanced process can play a larger role in gesture recognition-based UX for many wearable and smart devices.

• Nuclear Engineering and Technology
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• 제55권3호
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• pp.981-988
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• 2023

A seismic event caused an accident at the Fukushima Nuclear Power Plant, which further resulted in simultaneous accidents at several units. Consequently, this incident has aroused great interest in the safety of nuclear power plants worldwide. A reasonable safety evaluation of such an external event should appropriately consider the correlation between SSCs (structures, systems, and components) and the probability of failure. However, a probabilistic safety assessment in current nuclear industries is performed conservatively, assuming that the failure correlation between SSCs is independent or completely dependent. This is an extreme assumption; a reasonable risk can be calculated, or risk-based decision-making can be conducted only when the appropriate failure correlation between SSCs is considered. Thus, this study analyzed the effect of the failure correlation of SSCs on the safety of the system to realize rational safety assessment and decision-making. Consequently, the impact on the system differs according to the size of the failure probability of the SSCs and the AND and OR conditions.

• 대한기계학회논문집B
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• 제37권1호
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• pp.75-79
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• 2013

반도체 수요의 폭발적인 증가와 기술의 진보로 단위면적당 소자수가 늘어나고 있다. 그에 따라 단위면적당 발열량이 더욱 높아져서 반도체의 수명과 신뢰성 보장을 위한 냉각문제의 해결이 점점 중요해지고 있다. 특히, 집적도를 높이기 위해 소자를 기판에 매립하는 chip-in-board (CIB) 패키지에서는 방열이 더욱 심각한 문제가 된다. 본 논문에서는 각기 다른 재질의 층으로 구성된 열 전달모형을 설정하고, 3 차원 열 전달 해석으로 적절한 경계 조건에 맞추어 계산하였다. 이를 토대로 발열량을 정량적으로 예측하여 실제모델에 적용 될 수 있는 설계자료로 이용하고자 한다.

• 한국산학기술학회논문지
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• 제17권10호
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• pp.609-617
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• 2016

일반적으로 보행자의 위치를 파악하는데 사용하는 시스템을 개인 항법 장치 (PNS: Personal Navigation System)라고 한다. 위성 항법 시스템(GPS: Global Positioning System)은 PNS의 대표적인 사례이나, GPS 위성 신호 수신이 어려운 지역에서는 적용이 어려운 단점이 있다. GPS 신호 음영지역에서의 위치정보를 획득하기 위한 방법으로서 보행자 관성 항법(PDR: Pedestrian Dead Reckoning)은 별도의 인프라 없이 관성측정장치(IMU: Inertial Measurement Unit)만을 이용하여 보행자의 위치를 추정하는 방식으로서 인프라 구축이 어려운 특수 분야에 적용이 적합한 방식이다. 본 논문에서는 전장환경과 같은 GPS가 제한되는 특수한 환경 하에서 보행자의 다양한 이동유형을 고려한 관성항법 기반의 보행자용 위치인식 기법을 제안한다. 걷기, 뛰기, 포복과 같은 다양한 이동 형태에 따른 보행 거리 추정을 위해 IMU에서 제공되는 센서의 정보를 활용하여 걸음 검출과 보폭 추정으로 구성되는 보행거리 추정 기법과 HDR 알고리즘과 EKF(Extended Kalman Filter) 기반의 보행방향 추정 기법을 제안한다. 또한 건물입구와 같은 GPS 신호가 수신이 되나 신뢰성이 떨어지는 구간에서의 GPS와 PDR간 위치정보 융합 기법을 제안한다. 제안 기법의 성능 검증을 위해 자체 위치인식 모듈을 제작하여 국외제품과 비교 실험을 실시하였다. 실험결과, 제안 기법은 약 600m의 이동경로에서 평균 위치오차 거리는 5.64m, 이동거리 오차율 3.41%의 결과를 보였다.

• 한국컴퓨터정보학회논문지
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• 제28권1호
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• pp.49-54
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• 2023

GPS(Global Positioning System)는 군사 목적으로 개발되었고, 민간인 신호(GPS L1주파수 C/A 신호)를 개방하면서 많은 발전이 이루어졌다. 현재의 위성은 하루 약 2회 주기로 지구를 공전하며 위치를 측정하는데 위성 신호 3개(초기에는 시각 오차까지 계산하기 위하여 4개)이상을 수신하는데 전파 출발 시간에서부터 수신된 위성 신호의 전파 도달 시간(TOA)까지의 데이터를 삼변측량 방식을 통해 지상 수신기 3차원 위치를 결정한다. 그러나 GPS를 활용한 내비게이션의 경우 보통 5~10m의 위치 오차가 발생하며 아파트와 실내, 터널, 공장지대 및 산악 지대 등, 많은 지역이 GPS의 사각지대 또는 오차 범위 밖의 무력화 지역으로 존재하고 있다. 따라서 GPS 위성 신호의 수신이 불가능한 지역에서 현재의 위치 정보를 획득하기 위해서는 다른 방안이 제시되어야 한다. 본 연구에서는 가속도와 자이로 센서가 결합된 IMU(Inertial Measurement Unit)와 지자기 센서를 이용하여 GPS 신호 수신이 불가능한 지형에서도 위치인식이 가능하도록 시스템을 설계 하였다. 9-DOF IMU와 지자기 센서를 이용한 순간 속도 값을 계산하여 현재의 위치를 추적할 수 있는 방안을 연구 하였으며 제작과 실험을 통해 그 타당성을 검증하였다.

• Molecules and Cells
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• 제44권10호
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• pp.770-779
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• 2021

Transgenic Arabidopsis thaliana expressing an anti-rabies monoclonal antibody (mAb), SO57, was obtained using Agrobacterium-mediated floral dip transformation. The endoplasmic reticulum (ER) retention signal Lys-Asp-Glu-Leu (KDEL) was tagged to the C-terminus of the anti-rabies mAb heavy chain to localize the mAb to the ER and enhance its accumulation. When the inaccurately folded proteins accumulated in the ER exceed its storage capacity, it results in stress that can affect plant development and growth. We generated T₁ transformants and obtained homozygous T₃ seeds from transgenic Arabidopsis to investigate the effect of KDEL on plant growth. The germination rate did not significantly differ between plants expressing mAb SO57 without KDEL (SO plant) and mAb SO57 with KDEL (SOK plant). The primary roots of SOK agar media grown plants were slightly shorter than those of SO plants. Transcriptomic analysis showed that expression of all 11 ER stress-related genes were not significantly changed in SOK plants relative to SO plants. SOK plants showed approximately three-fold higher mAb expression levels than those of SO plants. Consequently, the purified mAb amount per unit of SOK plant biomass was approximately three times higher than that of SO plants. A neutralization assay revealed that both plants exhibited efficient rapid fluorescent focus inhibition test values against the rabies virus relative to commercially available human rabies immunoglobulins. KDEL did not upregulate ER stress-related genes; therefore, the enhanced production of the mAb did not affect plant growth. Thus, KDEL fusion is recommended for enhancing mAb production in plant systems.

• 대한비뇨기종양학회지
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• 제16권3호
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• pp.110-118
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• 2018

Purpose: The aim of this study is to confirm the detection rate of transperineal biopsy after multiparametric magnetic resonance imaging (mpMRI) and compared it to that of transrectal biopsy. We also examined the role of mpMRI and the rate of complications for each method. Materials and Methods: In a retrospective study, we analyzed 147 patients who underwent mpMRI before prostate biopsy because of elevated serum prostate-specific antigen and/or abnormal digital rectal examination findings at Korea University Hospital, Seoul, Korea from March 2017 to April 2018. Regions on the mpMRI that were suggestive of prostate cancer were categorized according to the Prostate Imaging-Reporting and Data System (PI-RADS v2). For transperineal biopsy, a 20-core saturation biopsy was performed by MRI-TRUS cognitive or fusion techniques and a 12-core biopsy was performed in transrectal biopsy. Results: Sixty-three and 84 patients were enrolled in transperineal group and transrectal group, respectively. The overall detection rate of prostate cancer in transperineal group was 27% higher than that in transrectal group. Classification according to PI-RADS score revealed a significant increase in detection rate in all patients, as the PI-RADS score increased. Frequency of complications using the Clavien-Dindo classifications revealed no significant differences in the total complications rate, but two patients in transrectal group received intensive care unit care due to urosepsis. Conclusions: Our results confirmed that transperineal biopsy is superior to transrectal biopsy for the detection of prostate cancer. From the complication point of view, this study confirmed that there were fewer severe complications in transperineal biopsy.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2012년도 제43회 하계 정기 학술대회 초록집
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• pp.80-81
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• 2012

Recently, one of the critical issues in the etching processes of the nanoscale devices is to achieve ultra-high aspect ratio contact (UHARC) profile without anomalous behaviors such as sidewall bowing, and twisting profile. To achieve this goal, the fluorocarbon plasmas with major advantage of the sidewall passivation have been used commonly with numerous additives to obtain the ideal etch profiles. However, they still suffer from formidable challenges such as tight limits of sidewall bowing and controlling the randomly distorted features in nanoscale etching profile. Furthermore, the absence of the available plasma simulation tools has made it difficult to develop revolutionary technologies to overcome these process limitations, including novel plasma chemistries, and plasma sources. As an effort to address these issues, we performed a fluorocarbon surface kinetic modeling based on the experimental plasma diagnostic data for silicon dioxide etching process under inductively coupled C4F6/Ar/O2 plasmas. For this work, the SiO2 etch rates were investigated with bulk plasma diagnostics tools such as Langmuir probe, cutoff probe and Quadruple Mass Spectrometer (QMS). The surface chemistries of the etched samples were measured by X-ray Photoelectron Spectrometer. To measure plasma parameters, the self-cleaned RF Langmuir probe was used for polymer deposition environment on the probe tip and double-checked by the cutoff probe which was known to be a precise plasma diagnostic tool for the electron density measurement. In addition, neutral and ion fluxes from bulk plasma were monitored with appearance methods using QMS signal. Based on these experimental data, we proposed a phenomenological, and realistic two-layer surface reaction model of SiO2 etch process under the overlying polymer passivation layer, considering material balance of deposition and etching through steady-state fluorocarbon layer. The predicted surface reaction modeling results showed good agreement with the experimental data. With the above studies of plasma surface reaction, we have developed a 3D topography simulator using the multi-layer level set algorithm and new memory saving technique, which is suitable in 3D UHARC etch simulation. Ballistic transports of neutral and ion species inside feature profile was considered by deterministic and Monte Carlo methods, respectively. In case of ultra-high aspect ratio contact hole etching, it is already well-known that the huge computational burden is required for realistic consideration of these ballistic transports. To address this issue, the related computational codes were efficiently parallelized for GPU (Graphic Processing Unit) computing, so that the total computation time could be improved more than few hundred times compared to the serial version. Finally, the 3D topography simulator was integrated with ballistic transport module and etch reaction model. Realistic etch-profile simulations with consideration of the sidewall polymer passivation layer were demonstrated.