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http://dx.doi.org/10.4014/jmb.1111.11044

Proteomic Comparison of Gibberella moniliformis in Limited-Nitrogen (Fumonisin-Inducing) and Excess-Nitrogen (Fumonisin-Repressing) Conditions

Choi, Yoon-E (LED Agri-bio Fusion Technology Research Center, Chonbuk National University)
Butchko, Robert A.E. (Bacterial Foodborne Pathogens and Mycotoxin Research Unit, USDA-ARS)
Shim, Won-Bo (Department of Plant Pathology and Microbiology, Program for the Biology of Filamentous Fungi, Texas A&M University)

Publication Information

Journal of Microbiology and Biotechnology / v.22, no.6, 2012 , pp. 780-787 More about this Journal

Abstract

The maize pathogen Gibberella moniliformis produces fumonisins, a group of mycotoxins associated with several disorders in animals and humans, including cancer. The current focus of our research is to understand the regulatory mechanisms involved in fumonisin biosynthesis. In this study, we employed a proteomics approach to identify novel genes involved in the fumonisin biosynthesis under nitrogen stress. The combination of genome sequence, mutant strains, EST database, microarrays, and proteomics offers an opportunity to advance our understanding of this process. We investigated the response of the G. moniliformis proteome in limited nitrogen (N0, fumonisin-inducing) and excess nitrogen (N+, fumonisin-repressing) conditions by one- and two-dimensional electrophoresis. We selected 11 differentially expressed proteins, six from limited nitrogen conditions and five from excess nitrogen conditions, and determined the sequences by peptide mass fingerprinting and MS/MS spectrophotometry. Subsequently, we identified the EST sequences corresponding to the proteins and studied their expression profiles in different culture conditions. Through the comparative analysis of gene and protein expression data, we identified three candidate genes for functional analysis and our results provided valuable clues regarding the regulatory mechanisms of fumonisin biosynthesis.

Keywords

Fusarium verticillioides; nitrogen metabolite repression; fumonisin regulation; proteomics; gene expression;

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