• Biomolecules & Therapeutics
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• v.14 no.3
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• pp.148-151
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• 2006

We have previously reported that 2.4 kb of L-plastin promoter (LP) could regulate the expression of adenoviral vector (AV) exogenous genes in a tumor cell specific manner. In the present study, we tested if the replication competent AdLPE1A vector results in a direct cytotoxic effect in hepatocelluar carcinoma (HCC) cells. In vitro cytotoxicity tests were carried out with replication-competent (AdLPE1A) and -incompetent (AdLPCD) LP-driven vectors. AdLPE1A is an AV in which LP was inserted 5' to the E1A and E1B genes. The AdLPCD vector contains LP and the E. coli cytosine deaminase (CD) gene in transcription unit. Exposure of cells to AdLPE1A generated a significant cytotoxic effect as compared to the control. Almost 90% of the cell had manifested the characteristic cytopatic effect on day 9 after infection of cells with 10 MOI of AdLPE1A. On the other hand, almost 35% of the cells were left when the cells had been treated with 100 MOI of AdLPCD together with 5-FC on day 9 when compared with the cells which had never been exposed neither 5-FC nor AdLPCD. These results showed that the replication competent AdLPE1A vector could kill the HepG2 cells directly by the oncolytic effect of the virus. The replication competent AV vector carrying viral E1A generated greater cytotoxic effect than the replication incompetent AV, which contains the CD prodrug activation transcription unit without E1A, in HepG2 cells.

• Journal of Broadcast Engineering
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• v.20 no.4
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• pp.533-544
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• 2015

Recently, most of the video services are usually transmitted in wireless networks. In networks environment, a packet of video is likely to be lost during transmission. For this reason, this paper proposes a new Error Concealment (EC) algorithm. For High Efficiency Video Coding (HEVC) bitstreams, the proposed algorithm includes Adaptive Prediction Unit-based Motion Vector Extrapolation (APMVE) and Boundary Matching (BM) algorithm, which employs both the temporal and spatial correlation. APMVE adaptively decides a Error Concealment Basic Unit (ECBU) by using the PU information of the previous frame and BM employing the spatial correlation is applied to only unreliable blocks. Simulation results show that the proposed algorithm provides the higher subjective quality by reducing blocking artifacts which appear in other existing algorithms.

• ETRI Journal
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• v.38 no.1
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• pp.195-205
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• 2016

This paper presents a novel follicular unit (FU) classification method based on an angle variation of a boundary vector according to the number of hairs in several FU images. The recently developed robotic FU harvest system, ARTAS, classifies through digital imaging the FU type based on the number of hairs with defects in the contour and outline profile of the FU of interest. However, this method has a drawback in that the FU classification is inaccurate because it causes unintended defects in the outline profile of the FU. To overcome this drawback, the proposed method classifies the FU's type by the number of variation points that are calculated using an angle variation a boundary vector. The experimental results show that the proposed method is robust and accurate for various FU shapes, compared to the contour-outline profile FU classification method of the ARTAS system.

• The Transactions of the Korea Information Processing Society
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• v.7 no.12
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• pp.3986-3994
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• 2000

A multiple vector reductive processing occurs during the vector inner product operation ([C] = [A] $\bigodot$,$\square$ [B]) and proceeds at the hardware dyadic pipeline unit. Every scalar result has to be generated with the component merging delay time in the multiple vector reduction($\bigodot$). In this paper we propose a new design method by which the component merging time could be eliminated from the multiple reduction and the scalar results from the reduction($\bigodot$) could be generated nearly in the almost same condensed time as the input components are fel>ded in the dyadic pipeline unitlo) or the output components are drained out of the dyadic pipeline unit($\square$), so called a dedicated chained pipeline unit for only a inner product operation.

• Journal of the Korean Mathematical Society
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• v.44 no.3
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• pp.605-613
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• 2007

Main interest of the present paper is to investigate the criticality of characteristic vector fields on almost cosymplectic manifolds. Killing critical characteristic vector fields are absolute minima. This paper contains some examples of non-Killing critical characteristic vector fields.

• BMB Reports
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• v.32 no.5
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• pp.497-501
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• 1999

Biotinylation of recombinant proteins is a powerful tool for the detection and analysis of proteins of interest in a large variety of assay systems. The recent development of in vivo biotinylation techniques in E. coli has opened new possibilities for the production of site-specifically biotinylated proteins without the need for further manipulation after the isolation of the recombinantly expressed proteins. In the present study, a novel vector set was generated which allows the convenient cloning and expression of proteins of interest fused with an N-terminal in vivo biotinylated thioredoxin (TRX) protein. These vectors were derived from the previously reported pBIOTRX vector into which was incorporated part of the pBluescript II+phagemid multiple cloning site (MCS), amplified by PCR using a pair of sophisticated oligonucleotide primers. The functionality of these novel vectors was examined in this system by recombinant expression of rat transforming growth factor-$\beta$. Western-blot analysis using TRX-specific antibodies or peroxidase-conjugated streptavidin confirmed the successful induction of the fusion protein and the in vivo conjugation of biotin molecules, respectively. The convenience of molecular subcloning provided by the MCS and the effective in vivo biotinylation of proteins of interest makes this novel vector set an interesting alternative for the production of biotinylated proteins.

• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.850-855
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• 2013

Finding new antimicrobial activities by functional metagenomics has been shown to depend on the heterologous host used to express the foreign DNA. Therefore, efforts are devoted to developing new tools for constructing metagenomic libraries in shuttle vectors replicatable in phylogenetically distinct hosts. Here we evaluated the use of the Escherichia coli-Bacillus subtilis shuttle vector pHT01 to construct a forest-soil metagenomic library. This library was screened in both hosts for antimicrobial activities against four opportunistic bacteria: Proteus vulgaris, Bacillus cereus, Staphylococcus epidermidis, and Micrococcus luteus. A new antibacterial activity against B. cereus was found upon screening in B. subtilis. The new antimicrobial agent, sensitive to proteinase K, was not active when the corresponding DNA fragment was expressed in E. coli. Our results validate the use of pHT01 as a shuttle vector and B. subtilis as a host to isolate new activities by functional metagenomics.

• Journal of the Korean Mathematical Society
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• v.32 no.3
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• pp.509-518
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• 1995

Let $M^n$ be a hypersurface in the Euclidean space $R^{n+1}$ with position vector field x and unit normal vector field G.

• The Transactions of the Korea Information Processing Society
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• v.3 no.1
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• pp.167-180
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• 1996

In the scalar processing oriented machine scalar operations must be performed for the vector processing as many as the number of vector components. So called a vector processing mechanism by the von Neumann operational principle. Accessing vector data hasto beperformed by theevery pointing ofthe instruction or by the address calculation of the ALU, because there is only a program counter(PC) for the sequential counting of the instructions as a memory accessing device. It should be here proposed that an access unit dimensionally to address components has to be designed for the compensation of the organizational hardware defect of the conventional concept. The necessity for the vector structuring has to be implemented in the instruction set and be performed in the mid of the accessing data memory overlapped externally to the data processing unit at the same time.

• The KIPS Transactions:PartA
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• v.12A no.3 s.93
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• pp.263-272
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• 2005

The addressing mode for data is performed by the software in yon Neumann-concept(SISD) computer a priori without hardware design of an address counter for operands. Therefore, in the addressing mode for the vector the corresponding variables as much as the number of the elements should be specified and used also in the software method. This is because not for operand but only for an instructions, quasi PC(program counter) is designed in hardware physically. A vector has a characteristic of a structural dimension. In this paper we propose to design a hardware unit physically external to the CPU for addressing only the elements of a vector unit with the structure and dimension. Because of the high speed performance for a vector processing it should be designed in the SIMD pipeline mechanics. The proposed mechanics is evaluated through a simulation. Our result shows $12\%$ to $30\%$ performance enhancement over CRAY architecture under the same hardware consideration(processing unit).