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http://dx.doi.org/10.4014/jmb.1212.12008

Bacillus subtilis as a Tool for Screening Soil Metagenomic Libraries for Antimicrobial Activities  

Biver, Sophie (Microbiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liege)
Steels, Sebastien (Microbiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liege)
Portetelle, Daniel (Microbiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liege)
Vandenbol, Micheline (Microbiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liege)
Publication Information
Journal of Microbiology and Biotechnology / v.23, no.6, 2013 , pp. 850-855 More about this Journal
Abstract
Finding new antimicrobial activities by functional metagenomics has been shown to depend on the heterologous host used to express the foreign DNA. Therefore, efforts are devoted to developing new tools for constructing metagenomic libraries in shuttle vectors replicatable in phylogenetically distinct hosts. Here we evaluated the use of the Escherichia coli-Bacillus subtilis shuttle vector pHT01 to construct a forest-soil metagenomic library. This library was screened in both hosts for antimicrobial activities against four opportunistic bacteria: Proteus vulgaris, Bacillus cereus, Staphylococcus epidermidis, and Micrococcus luteus. A new antibacterial activity against B. cereus was found upon screening in B. subtilis. The new antimicrobial agent, sensitive to proteinase K, was not active when the corresponding DNA fragment was expressed in E. coli. Our results validate the use of pHT01 as a shuttle vector and B. subtilis as a host to isolate new activities by functional metagenomics.
Keywords
Antimicrobial activity; Bacillus cereus; Bacillus subtilis; functional metagenomics; shuttle vector;
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