• Journal of Astronomy and Space Sciences
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• v.28 no.4
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• pp.333-344
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• 2011

An optical tracking system has advantages for observing geostationary earth orbit (GEO) satellites relatively over other types of observation system. Regular surveying for unidentified space objects with the optical tracking system can be an early warning tool for the safety of five Korean active GEO satellites. Two strategies of positioning on the observed image of Communication, Ocean and Meteorological Satellite 1 are tested and compared. Photometric method has a half root mean square error against streak method. Also eccentricity method for initial orbit determination (IOD) is tested with simulation data and real observation data. Under 10 minutes observation time interval, eccentricity method shows relatively better IOD results than the other time interval. For follow-up observation of unidentified space objects, at least two consecutive observations are needed in 5 minutes to determine orbit for geosynchronous orbit space objects.

• Journal of Microbiology and Biotechnology
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• v.21 no.1
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• pp.5-13
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• 2011

In this study, seven Trichoderma species (33 strains) were classified using secondary metabolite profile-based chemotaxonomy. Secondary metabolites were analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS-MS) and multivariate statistical methods. T. longibrachiatum and T. virens were independently clustered based on both internal transcribed spacer (ITS) sequence and secondary metabolite analyses. T. harzianum formed three subclusters in the ITS-based phylogenetic tree and two subclusters in the metabolitebased dendrogram. In contrast, T. koningii and T. atroviride strains were mixed in one cluster in the phylogenetic tree, whereas T. koningii was grouped in a different subcluster from T. atroviride and T. hamatum in the chemotaxonomic tree. Partial least-squares discriminant analysis (PLS-DA) was applied to determine which metabolites were responsible for the clustering patterns observed for the different Trichoderma strains. The metabolites were hetelidic acid, sorbicillinol, trichodermanone C, giocladic acid, bisorbicillinol, and three unidentified compounds in the comparison of T. virens and T. longibrachiatum; harzianic acid, demethylharzianic acid, homoharzianic acid, and three unidentified compounds in T. harzianum I and II; and koninginin B, E, and D, and six unidentified compounds in T. koningii and T. atroviride. The results of this study demonstrate that secondary metabolite profiling-based chemotaxonomy has distinct advantages relative to ITS-based classification, since it identified new Trichoderma clusters that were not found using the latter approach.

• Journal of fish pathology
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• v.23 no.1
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• pp.131-135
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• 2010

Unidentified viral disease with high mortalities has been recorded consistently since 1998 in cultured carp Cyprinus carpio in Korea. In the present study, we investigated the histopathological characteristics of the diseased carp to evaluate the relatedness to koi herpesvirus disease (KHVD). Histopathogical examination revealed severe necrotic changes and vacuous cells in the kidney, spleen, liver, pancreas, heart and intestine, but eosinophilic intranuclear inclusion, a typical characteristic of KHV infection was not detected. These results suggest that KHVD may be not a cause for the high mortality occurring among cultured carp in Korea and possibly other unknown viral disease could are related.

• Journal of Aquaculture
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• v.21 no.3
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• pp.139-145
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• 2008

Morphological classification of echinoid species has many difficulties because of their phenotypic variations. In the present study, we analyzed morphotypes and partial mitochondrial 12S rDNA sequences of four sea urchin species classified as Pseudocentrotus depressus, Anthocidaris crassispina, Hemicentrotus pulcherrimus and Strongylocentrotus nudus, and unidentified four species collected from the coasts of the East sea. Their genomic DNAs were extracted from gonads and mitochondrial 12S rDNA sequences were amplified by the polymerase chain reaction (PCR) method. The sequence identities among the known four sea urchin species were 87.4-95.6%. The sequence identities among the unidentified four species were 99.4-99.6% and showed the highest homology to S. intermedius(99.8%). Thus, our phylogenetic tree indicates that the unidentified four species belong to S. intermedius.

• Journal of Microbiology and Biotechnology
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• v.32 no.12
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• pp.1553-1560
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• 2022

A Gram-stain-negative, rod-shaped bacterial strain, JC4^T, was isolated from a freshwater sample and determined the taxonomic position. Initial identification based on 16S rRNA gene sequences revealed that strain JC4^T is affiliated to the genus Mucilaginibacter with a sequence similarity of 97.97% to Mucilaginibacter rigui WPCB133^T. The average nucleotide identity and digital DNA-DNA hybridization values between strain JC4^T and Mucilaginibacter species were estimated below 80.92% and 23.9%, respectively. Strain JC4^T contained summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c) and iso-C_15:0 as predominant cellular fatty acids. The dominant polar lipids were identified as phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, and two unidentified lipids. The respiratory quinone was MK-7. The genomic DNA G+C content of strain JC4^T was determined to be 42.44%. The above polyphasic evidences support that strain JC4^T represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter aquariorum sp. nov. is proposed. The type strain is JC4^T (= KCTC 92230^T = LMG 32715^T).

• Journal of the Korean Society of Marine Environment & Safety
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• v.19 no.4
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• pp.309-314
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• 2013

Temporal and spatial variations and species composition of ichthyoplanktons were investigated by Bongo net in a sea area with the construction of artificial upwelling structure at 13, June, 21 September, and 23 September in 2012. A total of fish eggs was 4,849 ind./$1,000m^3$, and fish larvaes was 641 ind./$1,000m^3$ in the study area. The common fish eggs was Engraulis japonicus which was composed of 57.5%, and unidentified I, II, III and IV were occurred with decreased number of individuals order. For fish larvaes, Pictiblennius yatabei, Repomucenus sp.A, sp.B and Scomber japonicus were dominated, however, other species were Engraulis japonicus, Konosirus puntatus, and unidentified I, II. Temporal variations of fish eggs and larvae were remarkable differed. Higher numbers of fish eggs and larvaes were July 2012, whereas those were lower at August 2012. Spatial variations of those were also significantly differed with higher numbers were at station 2 and 3 during study periods. The total number of fish eggs and larvae was about 4 fold against numbers of those in the compared area far from study area. These results indicated that higher numbers of fish eggs and larvaes were due to influx much more nutrients and food organisms with artificial upwelling.

• Clinical and Experimental Pediatrics
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• v.52 no.5
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• pp.567-575
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• 2009

Purpose : Epilepsy affects more than 0.5% of the world's population. It has a large genetic component and is caused by electrical hyperexcitability in the central nervous system. Despite its prevalence, the disease lacks definitive diagnostic serological biomarkers. To identify potential biomarkers for epilepsy by a convenient method, we analyzed the expression of serum proteins, reflecting alterations in the patient's proteomes. Methods : We compared two-dimensional electrophoretic band patterns of human sera from eight patients with temporal lobe epilepsy (TLE) with those of eight control subjects. The differentially expressed bands were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and electrospray ionization quadrupole time-of-flight mass spectrometry. esults : Twelve proteins were differentially expressed in the TLE group, of which 6 were identified. Expression of haptoglobin Hp2, PRO2675, immunoglobulin heavy chain constant region gamma 2, an unnamed protein, and three unidentified proteins were upregulated in serum from the patients with TLE, whereas those of major histocompatibility complex (MHC) class I antigen, plasma retinol-binding protein precursor, and three unidentified proteins were downregulated in these patients. After resection of the epileptogenic zone, the expressions of MHC class I antigen, immunoglobulin heavy chain constant region gamma 2, two of the downregulated unidentified proteins, and one of the upregulated unidentified proteins returned to the normal range. Conclusion : The 12 serum proteins in this study are potentially useful biomarkers for the diagnosis and monitoring of TLE.

• Parasites, Hosts and Diseases
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• v.38 no.1
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• pp.25-27
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• 2000

In an attempt to find the worm producing unidentified egg, one minute fluke was collect from a Korean patient after praziquantel administration. The fluke was identified to be Stellantchasmus falcatus by the expulsor. Brackish water fish was suggested to be a probable source of the infection.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.439-440
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• 2005

• Journal of Cardiovascular Imaging
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• v.31 no.3
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• pp.155-157
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• 2023