• Title/Summary/Keyword: UV-damage

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Production of clothes for beach volleyball players: Safe against ultraviolet radiation damage

  • He Huang
    • Geomechanics and Engineering
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    • v.32 no.6
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    • pp.627-637
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    • 2023
  • Volleyball is an international sport with many fans. This sport has made significant progress in schools and clubs. Volleyball is suitable for all age groups and can be used in different environments. It has many social and physical benefits. During the game provides special physical training for the players and is considered one of the most exciting games. Another type of volleyball is beach volleyball, a beach sport and one of the Olympic sports held on the sand with the same rules as volleyball. This sport is usually played in coastal areas, especially with wide sandy beaches. Because this sport is played in open spaces, the players stay in this space for a long time and are exposed to dangerous ultraviolet radiation. It is a wavelength of light in the range of electromagnetic waves with a wavelength between 10 and 400 nm. This wavelength is shorter than visible light and more protracted than X-ray. Ultraviolet (UV) rays are naturally present in sunlight and include about 10% of all waves emitted from the sun's surface. Prolonged exposure to ultraviolet light causes acute and chronic damage to the skin and vision and even destroys the entire immune system. Different covers of the earth's surface reflect different amounts of UV rays. For example, snow cover, sand, and seawater surface reflect this radiation. Therefore, the health of volleyball players is in danger due to this harmful radiation. This work aims to introduce a type of clothing made of nanoparticles that can repel ultraviolet rays and protect beach volleyball players whose health is at risk from this radiation.

Photoprotection effect of Pu'er tea and Curcuma longa L. extracts against UV and blue lights

  • Doyeong Son;Ji-Su Jun;KwangWon Hong
    • Journal of Applied Biological Chemistry
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    • v.66
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    • pp.106-113
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    • 2023
  • Plant extracts have been studied due to their potential as photoprotective agents against UV and blue light exposure. Previous studies have revealed that several plant extracts have photoprotection capacities and synergistic effects with synthetic products. However, such results for pu'er tea and Curcuma longa L. have not been reported yet for a cosmetic formulation. Thus, the objective of this study was to evaluate photoprotection capacities of pu'er tea and C. longa L. extracts for a sunscreen compound. The pu'er tea extract improved sun protection factor value of 2-ethyl-hexyl methoxycinnamate (a synthetic sunscreen compound) by 46% and showed a high antioxidant capability that could help skin recover from photo-induced damage. C. longa L. extract also showed a potential to protect skin from blue light-induced damage because it not only had a maximum absorption peak at the blue light range, but also protected human fibroblasts from blue light-induced damage. The addition of both extracts shifted the critical wavelength of 2-ethyl-hexyl methoxycinnamate from 350 nm to 386 nm, giving it a broad-spectrum feature. Thus, pu'er tea and C. longa L. extracts may enhance the photoprotection ability of synthetic sunscreen products.

Direct Evidence for the Radioprotective Effect of Various Carbohydrates on Plasmid DNA and Escherichia coli Cells

  • Ryu, Hwa-Ja;Yi, Kyung-Eun;Kim, Do-Won;Jung, Yun-Dae;Chang, Suk-Sang;Seo, Eun-Seong;Lee, Ki-Young;Marceau-Day, M.L.;Kim, Do-Man
    • Journal of Microbiology and Biotechnology
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    • v.12 no.4
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    • pp.598-602
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    • 2002
  • Damage to cells exposed to radiation is primarily attributed to direct effects on the structure of cellular DNA. Radiation-induced damage of pBluescript SK plasmid DNA and Escherichia coli $DH5\alpha$ were examined in the presence of various branched oligosaccharides, polysaccharides, and/or 8-MOP (8-methoxypsoralen). Branched oligosaccharides efficiently protected DNA and cells exposed to ultrasoft X-ray and UV irradiation. In the presence of 0.2% (w/v) branched oligosaccharides and polysaccharides, DNA can be protected from damage due to W and ultrasoft X-ray by a factor of 1.3-2.1 fo1d and 3.2-8.3 fold, respectively. The protective effect of cells exposed to UV or ultrasoft X-ray was also observed by branched oligosaccharides. The combination of MOP, a photoreagent, with carbohydrates increased the protective effects for DNA and cells, compared with that of a single use of MOP or carbohydrate alone.

The novel gene LRP15 is regulated by DNA methylation and confers increased efficiency of DNA repair of ultraviolet-induced DNA damage

  • Xu, Zhou-Min;Gao, Wei-Ran;Mei, Qi;Chen, Jian;Lu, Jing
    • BMB Reports
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    • v.41 no.3
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    • pp.230-235
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    • 2008
  • LRP15 is a novel gene cloned from lymphocytic cells, and its function is still unknown. Bioinformatic data showed that LRP15 might be regulated by DNA methylation and had an important role in DNA repair. In this study, we investigate whether the expression of LRP15 is regulated by DNA methylation, and whether overexpression of LRP15 increases efficiency of DNA repair of UV-induced DNA damage in HeLa cells. The results showed (1) the promoter of LRP15 was hypermethylated in HeLa cells, resulting a silence of its expression. Gene expression was restored by a demethylating agent, 5-aza-2'-deoxycytidine, but not by a histone deacetylase inhibitor, trichostatin A; (2) overexpression of LRP15 inhibited HeLa cell proliferation, and the numbers of cells in the G2/M phase of the cell cycle in cells transfected with LRP15 increased about 10% compared with controls; (3) cyclin B1 level was much lower in cells overexpressing LRP15 than in control cells; and (4) after exposure to UV radiation, the LRP15-positive cells showed shorter comet tails compared with the LRP15-negative cells. From these results we conclude that the expression of LRP15 is controlled by methylation in its promoter in HeLa cells, and LRP15 confers resistance to UV damage and accelerates the DNA repair rate.

The Measurement and Analysis for Ultraviolet Radiation (자외선복사의 측정과 분석)

  • Han, Jong-Sung;Kim, Hong-Bum;Kim, Hoon
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.19 no.2
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    • pp.7-12
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    • 2005
  • When a light is projected upon a material, part of its radiant energy is absorbed and the rest is reflected or transmitted according to the nature of the material. The molecules of the substance absorbing a light obtains the radiant energy to make thermal reactions or photochemical reactions. Specially, photochemical reactions by ultraviolet(UV) radiation brings about photochemical degradation such as color change. Because the photochemical damage brings about a devaluation of material and once damaged, it is irretrievable, it is necessary to minimize the damage and conserve the native quality of a material by a protective lighting system. This paper presents the measurement and photochemical damage for UV radiation from light sources. We measured the optical radiations from artificial light sources and daylight, and we analyzed a functional relation between irradiance and UV radiation form the results.

Effects of Radioprotectors on DNA Repair Capacity of Tumor Cells

  • Kim, Choon-Mi;Kim, Mi-Kyung
    • Archives of Pharmacal Research
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    • v.16 no.4
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    • pp.259-264
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    • 1993
  • Three cell lines, CHO, L929 and B16 which are non-tumorigenic and cancer cells, respectively, were first tested for their survival in the presence of radioprotective ginseng protein fraction(GPF0. The influence of three radioprotectors-CPF, cysteamine, and 1-Methyl-2-bis[(2-methylthio)vinyl] quinolinium iodide (MVQI) on DNA repair capacity of UV damaged cells survival test, the GPF showed higher cytotoxicity in L929 and B16 than in CHO cells. However, the degree of cell killing was also investigated by measuring $^3H$-thymidine incorporation of PUVA treated cells. In cell survival test, the GPF showed higher cytotoxicity in L929 and B16 than in CHO cells. However, the degree of cell killing was not high enough to consider it as an antitumorigenic agent. Variable results were obtained in the effects on DNA repair capacity depending on the protectors and cell lines used. In pretreatment, the presence of GPF and MVOI brought about a sinificant increase in the capacity in both CHO and B16 cells. However, in L929, the enhancing effect was not shown. In all three cell lines, cysteamine showed lower repair capacity than control, suggesting the primary damage reduction in stronger enhancing effects in L929 and B16 cells, while it was weaker in CHO cells. Here also cystemine hsowed a very little or no increase in the capacity in all three cell lines. These results demonstrate that GPF has mild cytotoxicity in tumorignic cells and that GPF and MVQI enhance DNA repair capacity of UV damaged cells, whether they are tumorigenic or not. On the other hand, cysteamine shows only damage reduction effect. Celles of different genetic origin seem to give different responses to the modifier and different modifiers may possibly work by different mechanisms.

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Characterization of UV-Inducible Gene(UVI-155) in Schizosaccharomyces pombe (효모 Schizosaccharomyces pombe에서 자외선 유도유전자 UVI-155의 분리 및 특성 연구)

  • Jin, Ji-Young;Choi, In-Soon
    • Journal of Life Science
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    • v.16 no.1
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    • pp.126-130
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    • 2006
  • The present study intends to characterize the DNA damage-inducible responses in yeast. The fission yeast, Schizosaccharomyces pombe was used in this study as a model system for higher eukaryotes. To study UV-inducible responses in S. pombe, five UV-inducible cDNA clones were isolated from S. pombe by using subtration hybridization method. To investigate the expression of isolated genes, UVI-155, the cellular levels of the transcripts were determined by Northern blot analysis after UV-irradiation. The transcripts of isolated gene (UVI-155) increased rapidly and reached maximum accumulation after UV-irradiation. Compared to the message levels of control, the levels of maximal increase were approximately 5 fold to UV-irradiation. In order to investigation whether the increase of UVI-l55 trascripts was a specific results of UV-irradiation, UVI-155 transcript levels were examined after treating the cells to mthylmethane sulfonate (MMS). The transcripts of UVI-155 were not induced by treatment of $0.25\%$ MMS. These results implied that the effects of damaging agents are complex and different regulatory pathways exist for the induction of these genes. To characterize the UVI-155 gene, gene deletion experiments were analyzed. The deleted strain was not well grown. This result indicated that the UVI-155 gene is essential for cell viability.

A Study on the Performance of Surface UV Printing Device for Power Indicator Production (파워인덕터 생산용 표면 UV 인쇄장치 성능 연구)

  • Hyun-Mu Lee;So-Mi An;Sung-Min Ahn;Jeong-Hwan Seo;Byoung-Jo Jung;Sung-Lin Kang
    • Journal of Advanced Technology Convergence
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    • v.2 no.4
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    • pp.1-6
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    • 2023
  • Research on power inductor surface UV printing equipment using cylindrical magnets can prevent damage to quality consumable materials (making plates, Squeegees) during printing and improve printing quality by applying technology to prevent product from flipping or standing up when fixing the product by making the magnetic formation of cylindrical magnets form up and down. The development of cylindrical magnets that changed the direction of magnetic force will stabilize the fixing method for metal products made by powder compression, increasing the production capacity for small products. Finally, by studying the power inductor surface UV printing device using cylindrical magnets, it can be differentiated from the spray and deeping methods that were being worked on, production will be greatly improved, and as a result, cost reduction and competitive production will be possible.

Photoprotective Effects of Silybum marianum Extract (흰무늬엉겅퀴 열매 추출물의 자외선에 대한 피부 보호 효과)

  • Kim, Daehyun;Bae, Woo Ri;Kim, Yun-Sun;Shin, Dong-won;Park, Sun-Gyoo;Kang, Nae-Gyu
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.45 no.2
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    • pp.209-216
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    • 2019
  • Ultraviolet rays (UV) cause photoaging by inducing skin photodamages such as erythema and sunburn. Silymarin is a mixture of antioxidant polyphenols extracted from Silybum marianum fruit (S. m), which is known as milk thistle. It is known to protect skin tissues from UV treatment and antioxidant effects. In this study, we aimed to identify the photoprotective effects of S. m extract, which has silymarin in the epidermis layer of the skin. We found that the extract can function as a UV filter, so it can reduce DNA damage by UV treatment. Especially, we found that, in the stratum corneum, the extract can suppress the protein carbonylarion and DNA damages caused by suberythemal dose of UV treatment which does not induce erythema in the skin. UV treatment also increased protein carbonylation levels in the stratum corneum by oxidation, but it was prevented by applying the extract. The extract can absorb UV with minimal phototoxicity. Together, our study suggests that S. m extract can be used as a photo-protective ingredient to avoid photoaging of the skin.

Ultraviolet B (UVB) Induces Down-regulation of Parkin Gene Expression

  • Kim, Sung Hoon;Kang, Yeo Wool;Lee, Juyeon;Kim, Hyun-Kyung;Jung, Byung Chul;Kim, Bohee;Kim, Dai Joong;Kim, Yoon Suk
    • Biomedical Science Letters
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    • v.22 no.1
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    • pp.18-23
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    • 2016
  • Ultraviolet (UV) irradiation induces cellular damage. A variety of cellular responses for repairing cellular damage including DNA damage occur after UV irradiation. During the repair processes, expression and activation of various molecules are regulated depending on the types of cellular damage. Parkin is an E3 ligase and act as a tumor suppressor. Recently, it has been reported that Parkin is involved in the DNA repair process. In the current study, we investigated whether UVB irradiation influences expression of Parkin. Parkin expression transiently decreased after UVB irradiation both at the mRNA and protein levels, but returned to normal levels thereafter. Taken together with cell viability data, Parkin expression is down-regulated during UVB-induced suppression of cell growth and is increased again in accordance with recovery of UVB-induced cell growth inhibition. However, Parkin overexpression or knockdown did not influence UVB-induced cell growth inhibition and recovery. We propose that Parkin could be a useful molecular marker for evaluating conditions of cells after UVB irradiation.