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Direct Evidence for the Radioprotective Effect of Various Carbohydrates on Plasmid DNA and Escherichia coli Cells  

Ryu, Hwa-Ja (Department of Fine Chemical Engineering, Chonnam National University)
Yi, Kyung-Eun (Department of Faculty of Applied Chemical Engineering, Chonnam National University)
Kim, Do-Won (Department of Physics, and East Coastal Marine Bioresources Research Center, Kangnung National University)
Jung, Yun-Dae (Department of Physics, Kangnung National University)
Chang, Suk-Sang (Pohang Accelerator Laboratory, Pohang University of Science and Technology)
Seo, Eun-Seong (Department of Materials and Biochemical Engineering, Chonnam National University)
Lee, Ki-Young (Department of Faculty of Applied Chemical Engineering, Chonnam National University)
Marceau-Day, M.L. (Center for Advanced Microstructures and devices, Louisiana State University)
Kim, Do-Man (Department of Faculty of Applied Chemical Engineering, Chonnam National University)
Publication Information
Journal of Microbiology and Biotechnology / v.12, no.4, 2002 , pp. 598-602 More about this Journal
Abstract
Damage to cells exposed to radiation is primarily attributed to direct effects on the structure of cellular DNA. Radiation-induced damage of pBluescript SK plasmid DNA and Escherichia coli $DH5\alpha$ were examined in the presence of various branched oligosaccharides, polysaccharides, and/or 8-MOP (8-methoxypsoralen). Branched oligosaccharides efficiently protected DNA and cells exposed to ultrasoft X-ray and UV irradiation. In the presence of 0.2% (w/v) branched oligosaccharides and polysaccharides, DNA can be protected from damage due to W and ultrasoft X-ray by a factor of 1.3-2.1 fo1d and 3.2-8.3 fold, respectively. The protective effect of cells exposed to UV or ultrasoft X-ray was also observed by branched oligosaccharides. The combination of MOP, a photoreagent, with carbohydrates increased the protective effects for DNA and cells, compared with that of a single use of MOP or carbohydrate alone.
Keywords
Radiation protection; oligosaccharides; UV; ultrasoft X-ray; Escherichia coli;
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