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http://dx.doi.org/10.5352/JLS.2006.16.1.126

Characterization of UV-Inducible Gene(UVI-155) in Schizosaccharomyces pombe  

Jin, Ji-Young (Department of Biological Science, Silla University)
Choi, In-Soon (Department of Biological Science, Silla University)
Publication Information
Journal of Life Science / v.16, no.1, 2006 , pp. 126-130 More about this Journal
Abstract
The present study intends to characterize the DNA damage-inducible responses in yeast. The fission yeast, Schizosaccharomyces pombe was used in this study as a model system for higher eukaryotes. To study UV-inducible responses in S. pombe, five UV-inducible cDNA clones were isolated from S. pombe by using subtration hybridization method. To investigate the expression of isolated genes, UVI-155, the cellular levels of the transcripts were determined by Northern blot analysis after UV-irradiation. The transcripts of isolated gene (UVI-155) increased rapidly and reached maximum accumulation after UV-irradiation. Compared to the message levels of control, the levels of maximal increase were approximately 5 fold to UV-irradiation. In order to investigation whether the increase of UVI-l55 trascripts was a specific results of UV-irradiation, UVI-155 transcript levels were examined after treating the cells to mthylmethane sulfonate (MMS). The transcripts of UVI-155 were not induced by treatment of $0.25\%$ MMS. These results implied that the effects of damaging agents are complex and different regulatory pathways exist for the induction of these genes. To characterize the UVI-155 gene, gene deletion experiments were analyzed. The deleted strain was not well grown. This result indicated that the UVI-155 gene is essential for cell viability.
Keywords
UVI-155; UV-inducible gene; DNA repair; cell viability; MMS;
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1 Perozzi, G. and S. Prakash. 1986. RAD7 gene of Saccharomyses cerevisiae: transcript, nucleotide sequence analysis and functional relationship between the RAD7 and RAD23 gene products. Mol. Cell. Biol. 6, 1497-1507   DOI
2 Sung, P., S. Prakash and L. Prakash. 1982. The RAD6 protein of Saccharomyces cerevisiae polyubiquitinates histones, and its acidic domain mediated the specificity. Genes. Dev. 2, 1476-1485   DOI   ScienceOn
3 Weinert, T. A. and L. H. Hartwell. 1990. Characterization of RAD9 of Saccharomyces cerevisiae and evidence that its function acts posttranslationally in cell cycle arrest after DNA damage. Mol. Cell. Biol. 54, 6564-6572
4 Praekelt, U. M. and P. A. Macock. 1990. HSP12, a new small heat shock gene of Saccharomyces cerevisiae : analysis of structure, regulation and function. Mol. Gen. Genet. 233, 97-106
5 Morrison, A., E. J. Miller and L. Prakash. 1988. Domain structure and functional analysis of the carboxyl-terminal polyacidic sequence of the RAD6 protein of Saccharomyces cerevisiae. Mol. Cell Biol. 8, 1179-1185   DOI
6 Jang, Y. K., Y. H. Jin, M. Kim, F. Fabre. S. H. Hong and S. D. Park. 1998. Molecular cloning of $rhp51^+$ gene in Schizosccharomyces pombe, whose amino acid sequence is highly conserved from prokarytic RecA to the mammalian Rad51 homolog. Gene. 5, 130-142
7 Madura, K., and S. Prakash. 1990. Transcript levels of the Saccharomyces cerevisiae DNA repair gene RAD23 increase in response to UV light and in meiosis but remain constant in the mitotic cell cycle. Necleic Acid Res. 18, 4737-4742   DOI
8 Maga, J. A., T. A. McClanahan and K, McEntee. 1986. Transcriptional regulation of DNA damage responsive (DDR) genes in different rad mutant strains of Saccharomyces cerevisiae. Mol. Gen. Genet. 205, 276-284   DOI
9 McClanahan, T. and K. McEntee. 1986. DNA damage and heat shock dually regulated genes in Saccharomyces cerevisiae. Mol Cell. Biol. 6, 90-95   DOI
10 Montelone, B. A., S. Prakash and L. Prakash. 1981. Recombination and mutagenesis in rad6 mutants of Saccharomyces cerevisiae : Evidence for multiple functions of the RAD6 gene. Mol. Gen. Genet. 184, 410-415   DOI
11 Phipps, J., A. Nasim and D. R. Miller. 1985. Recovery, repair, and mutagenesis in Schizosaccharomyces pombe. Adv. Genetics. 23, 1-72   DOI
12 Radman, M., G. Villani, S. Boiteux, A. R. Kinsella, B. W. Clickman and S. Spadari. 1978. Replication fidelity : mechanisms of mutation avoidance and mutation fixation. Cold Spring Harbor Symp. Quant, Biol. 43, 937-942
13 Sambrook, J. and D. W. Russell. 2001. Molecular Cloning. A laboratory mannual. Cold Spring Harbor
14 Harosh, I. and P. Deschavanne. 1989. The RAD3 gene is a member of the DEAH family RNA helicase-like protein. Nucleic Acids Res. 19, 6331   DOI   ScienceOn
15 Feinberg, A. P. and B. A. Vogelstein. 1984. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 137, 266-267   DOI   ScienceOn
16 Birkenbihl, R. P. and S. Subramani. 1992. Cloning and characterization of rad21 an essential gene of Schizosaccharomyces pombe involved in DNA double strand break repair. Nuclei Acid Res. 20, 6605-6611   DOI   ScienceOn
17 Choi, I. S.1999. Isolation and characterization of new family genes DNA damage in yeast. Environmental Mutagens & Carcinogens. 19(1), 28-33
18 Elledge, S. J. and R. W. Davis. 1987. Identification and isolation of the gene encoding the small submit of ribonucleotide reductase from Saccharomyces cerevisiae: DNA damage-inducible gene required for mototic viability. Mol. Cell. Biol. 7, 2783-2793   DOI
19 Fornace, A. J, D. W. Nebert, M. C. Hollander, J. D. Luethy, M. Papathanasiou, J. Fargnoli and N. J. Holbrook. 1989. Mammalian genes coordinately regulated by growth arrest signal and DNA-damaging agents. Mol. Cell. Biol. 9, 4196-4203   DOI
20 Schild, D., B. Konfort, C. Perez, W. Gish and R. K. Mortimer. 1983. Isolation and characterization of yeast DNA repair genes. I. Cloning of the RAD52 gene. Curr. Genet. 7, 85-92   DOI   ScienceOn
21 Boothmann, D. A., M. Meyers, N. Fukunaga and S. W. Lee. 1993. Isolation of X-ray-inducible transcripts from radioresistant human melanoma cells. Proc. Natl. Acid. Sci. USA. 90, 7200-7204