• Journal of Korean Neurosurgical Society
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• v.60 no.2
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• pp.130-137
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• 2017

Objective : Autophagy is one of the key responses of cells to programmed cell death. Memantine, an approved anti-dementia drug, has an antiproliferative effect on cancer cells but the mechanism is poorly understood. The aim of the present study was to test the possibility of induction of autophagic cell death by memantine in glioma cell lines. Methods : Glioma cell lines (T-98 G and U-251 MG) were used for this study. Results : The antiproliferative effect of memantine was shown on T-98 G cells, which expressed N-methyl-D-aspartate 1 receptor (NMDAR1). Memantine increased the autophagic-related proteins as the conversion ratio of light chain protein 3-II (LC3-II)-/LC3-I and the expression of beclin-1. Memantine also increased formation of autophagic vacuoles observed under a transmission electron microscope. Transfection of small interfering RNA (siRNA) to knock down NMDAR1 in the glioma cells induced resistance to memantine and decreased the LC3-II/LC3-I ratio in T-98 G cells. Conclusion : Our study demonstrates that in glioma cells, memantine inhibits proliferation and induces autophagy mediated by NMDAR1.

• The Journal of the Petrological Society of Korea
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• v.14 no.4 s.42
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• pp.251-263
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• 2005

Modern volcanoes, Laoheishan and Huoshaoshan, have erupted during $1720\~1721$ in the Wudalianchi volcanic group, NE china. They comprise scoria and spatter cones that consist of potassium-rich phono-tephritic pyroclastic deposits and lavas, and include wide lava flow fields. The Laoheishan scoria cone is a polygenetic multiple volcano that overlaps earlier and later edifices with more complicated internal structures produced in greater scale and in earlier time than the Huoshaoshan. There is a funnel-shaped crater in the center of the later edifice of the Laoheishan scoria cone. The Huoshaoshan spatter cone is a monogenetic simple volcano with a central pit crater. The volcanic sequences indicate eruption processes that followed a repeated pattern that progressed through 5 stages of explosive and effusive eruption including lava fountains and Strombolian eruptions in the Laoheishan, and a recognizable pattern of 2 stages that started with Strombolian eruption and progressed through lava effusion in the Huoshaoshan.

• Journal of Korean society of Dental Hygiene
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• v.18 no.2
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• pp.239-251
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• 2018

Objectives: The purpose of this study was to investigate the effects of dental hygiene students' clinical practice stress, satisfaction and major satisfaction on career preparation behaviors. Methods:This study was conducted targeting the dental hygiene students who have clinical practice experience. The final 305 subjects were analyzed using SPSS 22.0 program. Results: The correlation between clinical practice stress and career satisfaction and career preparation behavior decreases with the higher degree of clinical practice and career preparation behavior. Analysis results about the affecting factors on the level of satisfaction with major satisfaction factors showed the highest factor of preparation behavior, followed by clinical practice and clinical practice stress in order. Analysis results about the affecting factors on the showed the highest factor of preparation behavior, followed by clinical practice and clinical practice stress in order. Conclusions: The results of this study showed that the higher the satisfaction of clinical practice and the higher the satisfaction of career, the more positive the career preparation behavior. Therefore, it's recommended to support continuous education programs to be coherent with dental hygiene student's career preparation behavior based on reinforcing individual competency by obtaining self-confidence and satisfaction from clinical practice.

• The Journal of the Korea Contents Association
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• v.19 no.3
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• pp.236-251
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• 2019

The animation industry is an emerging and promising industry of the 21st century, characterized by a wide range of consumer segments, high market demand, and a long life cycle of products. The paper is aimed at examining the current situation of the animation industry of Korea, the U.S., China and Japan, analyzing the success factors and presenting measures for activation of problems in China's animation industry, and contributing to the promotion of China's animation policies in the future. To realize this goal, the study methods were adopted literature review by using Internet data, statistical data, reports, papers and academic journals which are related to the Chinese animation industry and presented methods for activation. The aviations as follows, firstly, it is suggested actively train creative men of animation for profession. Secondly, it is advised to encourage the creativity and originality of Chinese animation works through various ways. Thirdly, it is suggested to develop an industry chain of animation which is suitable for the market circumstances. Fourthly, it needs to expand its viewer and strengthen its reality in the form and content of animation works.

• Korean Journal of Organic Agriculture
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• v.13 no.4
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• pp.389-400
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• 2005

This study was conducted to investigate into the ecological environments and the soil microflora of purple-bracted plantain lily (Hosta longipes Matsumura) for wild vgetables. Native soil textures of purple-bracted plantain lily were in the order of sandy loam (SL) > loam (L) > clay loam (CL). pH in soil was relatively acid by 4.8, electric conductivity was 0.08mS/cm, and organic matter content was 0.08g/kg. CEC was measured by $100.8cmol^{(+)}kg^{-1}$ and available phosphate was 103.4mg/kg. Contents of exchangeable cations in terms of potassium, calcium, and magnesium were measured by $0.33cmol^{(+)}kg^{-1},\;2.26cmol^{(+)}kg^{-1},\;and\;0.87cmol^{(+)}kg^{-1}$, etc. Diurnal changes in the air temperature of the natives were 15 to $20^{\circ}C$, that temperature differential was relatively little compared with that in open field by 15 to $30^{\circ}C$. Relative humidity in the natives were much more humid by 60 to 80% compared with that in open feld by 35 to 85%. Light intensity in the natives and the open field at ten o'clock were $2,300{\mu}mol/m^2/sec.\;and\;1,750{\mu}mol/m^2/sec.$ Total number of soil microorganisms were $8.4{\times}10^7\;c.f.u./g$. Mycorrhizal spore densities over $500{\mu}m,\;355{\sim}500{\mu}m,\;251{\sim}354{\mu}m,\;107{\sim}250{\mu}m\;and\;45{\sim}106{\mu}m$ were 0.8, 1.3, 2.1, 38.1, and 110.0 respectively. Mycorrhizal root infections by vesicle and hyphae were 17% and 6%. However, arbuscules in the roots were not shown.

• Korean Journal of Heritage: History & Science
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• v.46 no.3
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• pp.212-228
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• 2013

Stone cultural heritages are repaired by the use of metal stiffeners. The problem is that this type of repair has been based on the experience of workers without specific guidelines and has caused various problems. This is to suggest the structural reinforcement and behavioral characteristics of metal rods to minimize the secondary damage of materials and have the specimens tested and verified to establish the guidelines on how to insert metal stiffeners. When only epoxy resin is applied to the cut surface, only 70% of the properties of the parent material are regenerated and it is required to structurally reinforce the metal stiffener for the remaining 30%. The metal rod is under the structural behavior after the brittle failure of stone material and the structural behavior does not occur when the metal stiffener is below 0.251%. When it accounts for over 0.5%, it achieves structural reinforcement, but causes secondary damage of parent materials. The appropriate ratio of metal stiffener for the stone material with the strength of $1,500kgf/cm^2$, therefore, should be between 0.283% and 0.377% of the cross section of attached surface to achieve reversible fracture and ductility behavior. In addition, it is more effective to position the stiffeners at close intervals to achieve the peak stress of metal rod against bending load and inserting the stiffener into the upper secions is not structurally supportive, but would rather cause damage of the parent material. Thus, most stiffeners should be inserted into the lower part and some into the central part to work as a stable tensile material under the load stress. The dispersion effect of metal rods was influenced by the area of reinforcing rods and unrelated to their diameter. However, it ensures stability under the load stress to increase the number of stiffeners considering the cross section adhered when working on large-scale structures. The development length is engineered based upon the diameter of stiffener using the following formula: $l_d=\frac{a_tf_y}{u{\Sigma}_0}$. Also, helically-threaded reinforcing rods should be used to perform the behaviors as a structural material.

• Korean journal of applied entomology
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• v.26 no.4 s.73
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• pp.251-255
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• 1987

This study was conducted to investigate the effects of cultural methods on infection by Rhizoctonia disease(Rhizoctonia solani $K\"{u}hn$) of potato (Solanum tuberosum L.). Two potato cultivars, i.e., Irish Cobbler and Superior were planted in 1984 at the Alpine Experiment Station and 4 cultural methods were used in Combinations or separately. The deeper planting showed the later germination of sprout. The degree of infection on underground stems didn't show any differences between the treatments, but the yield was higher in deeper planting. When seed tubers were planted on April 28 the underground stems showed severely discolourated epidermis comparing to later planting, consequently the yield was low. The glazed chitting tubers produced healthy sprouts comparing to the untreated tubers, consequently the yield was also increased. The density of sclerotia on tuber skin was remarkably increased between 6 to 15days of delayed digging after haulm killing. Underground stems of Irish Cobbler were easily discolourated by the disease, where-as the density of sclerotia was higher on tuberskin of Superior than of Irish Cobbler.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.8
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• pp.3495-3501
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• 2014

Melanoma-associated antigen (MAGE) family genes have been considered as potentially promising targets for anticancer immunotherapy. MAGED4 was originally identified as a glioma-specific antigen. Current knowledge about MAGED4 expression in glioma is only based on mRNA analysis and MAGED4 protein expression has not been elucidated. In the present study, we investigated this point and found that MAGED4 mRNA and protein were absent or very lowly expressed in various normal tissues and glioma cell line SHG44, but overexpressed in glioma cell lines A172,U251,U87-MG as well as glioma tissues, with significant heterogeneity. Furthermore, MAGED4 protein expression was positively correlated with the glioma type and grade. We also found that the expression of MAGED4 inversely correlated with the overall methylation status of the MAGED4 promoter CpG island. Furthermore, when SHG44 and A172 with higher methylation were treated with the DNA demethylating agent 5-aza-2'-deoxycytidine (5-AZA-CdR) reactivation of MAGED4 mRNA was mediated by significant demethylation in SHG44 instead of A172. However, 5-AZA-CdR treatment had no effect on MAGED4 protein in both SHG44 and A172 cells. In conclusion, MAGED4 is frequently and highly expressed in glioma and is partly regulated by DNA methylation. The results suggest that MAGED4 might be a promising target for glioma immunotherapy combined with 5-AZA-CdR to enhance its expression and eliminate intratumor heterogeneity.

• Food Science and Preservation
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• v.10 no.2
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• pp.246-251
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• 2003

Pretense produced by Bacillus subtilis PANH765 was purified from culture supernatant by using ammonium sulfate fractionation DEAE-cellulose ion exchange chromatography, and gel filtration with Sephacryl S 200 HR and Sepharose CL-6B. DEAE-cellulose ion exchange column chromatography, separated the pretense into one fraction. This fraction was further purified using Sephacryl S 200 HR and Sepharose CL-6B gel titration. The molecular mass of pretense was estimated to be 35.0 kDa by the SDS-PAGE and gel filtration using Sepharose CL-6B. The results indicated that the purified pretense are monomeric proteins. Specific activity and purification folds of pretense were 657 U/mg and 4.35, respectively. The optimum temperature, optimum pit stable at a temperature range and pH ranges for the purified protease were 65$^{\circ}C$, 7.05, 50 ∼ 75$^{\circ}C$ and 6.0 ∼ 7.5, respectively. The pretense activity was decreased by the presence of PMSF and DFP, which the protease activity was increased by the presence of Na$\^$+/, K$\^$+/, Mg$\^$2＋/ and NH$_4$$\^$+/ ions.

• Journal of Digital Convergence
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• v.19 no.3
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• pp.245-251
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• 2021

This study was to investigate the effect of the new surface treatment method of zirconia on the shear bond strength with resin cement. The zirconia specimens were classified according to the surface treatment. CON: non-treatment, HF: 10 minutes exposure to 9% HF, ZS15: Apply 15% ZrO2 slurry, ZS30: Apply 30% ZrO2 slurry, ZS50: Apply 50% ZrO2 slurry. The resin cement was layered on the surface treated zirconia, and the shear bond strength between the zirconia and the resin cement was measured after thermo-cycling. The statistical methods for shear bond strength were Kruskal-Wallis test, Mann-Whitney U test, and Bonferroni correction(α=.05/10=.005). ZS15, ZS30, and ZS50 groups treated with zirconia slurry showed higher shear bond strength than CON and HF groups(p<.05/10=.005). Within the limits of this study, the surface treatment using zirconia slurry increased the shear bond strength with resin cement. The new surface treatment method complements and improves the limitations of the adhesion of zirconia, so that various clinical applications of zirconia can be expected.