• Environmental Analysis Health and Toxicology
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• v.25 no.2
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• pp.111-120
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• 2010

DEPs (diesel exhaust particles) like any other particles can be also inhaled into lung to participate in a damaging reaction to the organ. Possible damages might be apoptosis and inflammatory responses to the cells in respiratory track. The aim of this study was cytotoxicity evaluation of DEPs from five in-use diesel vehicles using a murine macrophage cell (RAW 254.7). We found that most DEPs have a considerable cytotoxicity compared to the control and SRM 2975. When measured by MTT assay and extents of apoptosis, DEPs of two highmileage vehicles had higher toxicity than those of the other three low-mileage vehicles tested. Although mRNA expression level of TNF-${\alpha$ somewhat explains the trend of cytotoxicity and apoptosis, that of IL-1$\beta$ did not. Correlation studies among the extents of MTT assay, apoptosis, and TNF-$\alpha$ expression showed that the extents between apoptosis and TNF-$\alpha$ expression was most highly correlated (r=0.96). These results suggest that cytotoxicity of various DEPs could be compared easily by measuring the extent of apoptosis or TNF-$\alpha$ expression by DEPs.

• Smart Structures and Systems
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• v.13 no.6
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• pp.993-1014
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• 2014

In civil engineering, revolving structures (RS) are a unique structural form applied in innovative architecture design. Such structures are able to revolve around themselves or along a certain track. However, few studies are dedicated to safety design or health monitoring of RS. In this paper, a wireless dynamic sensing system is developed for RS, and field tests toward a large revolving auditorium are conducted accordingly. At first, a wheel-rail problem is proposed: The internal force redistributes in RS, which is due to wheel-rail irregularity. Then the development of the sensing system for RS is presented. It includes system architecture, network organization, vibrating wire sensor (VWS) nodes and online remote control. To keep the sensor network identifiable during revolving, the addresses of sensor nodes are reassigned dynamically when RS position changes. At last, the system is mounted on a huge outdoor revolving auditorium. Considering the influence of the proposed problem, the RS of the auditorium has been designed conservatively. Two field tests are conducted via the sensing system. In the first test, 2000 people are invited to act as the live load. During the revolving process, data is collected from RS in three different load cases. The other test is the online monitoring for the auditorium during the official performances. In the end, the field-testing result verifies the existence of the wheel-rail problem. The result also indicates the dynamic sensing system is applicable and durable even while RS is rotating.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.44 no.1
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• pp.40-49
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• 2007

In a visual driver-assistance system, separating moving objects from fixed objects are an important problem to maintain multiple hypothesis for the state. Color and edge-based tracker can often be 'distracted' causing them to track the wrong object. Many researchers have dealt with this problem by using multiple features, as it is unlikely that all will be distracted at the same time. In this paper, we improve the accuracy and robustness of real-time tracking by combining a color histogram feature with a brightness of Optical Flow-based feature under a Sequential Monte Carlo framework. And it is also excepted from Tracking as time goes on, reducing density by Adaptive Particles Number in case of the fixed object. This new framework makes two main contributions. The one is about the prediction framework which separating moving objects from fixed objects and the other is about measurement framework to get a information from the visual data under a partial occlusion.

• Journal of KIISE:Software and Applications
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• v.35 no.7
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• pp.452-461
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• 2008

Binary diffing is a method to find differences in similar binary executables such as two different versions of security patches. Previous diffing methods using flow information can detect control flow changes, but they cannot track constant value changes. Biffing methods using assembly instructions can detect constant value changes, but they give false positives which are due to compiling methods such as instruction reordering. We present a binary diffing method and its implementation named SCV which utilizes both structure and value information. SCV summarizes structure and constant value information from disassembled code, and matches the summaries to find differences. By analyzing a Microsoft Windows security patches, we showed that SCV found necessary differences caused by constant value changes which the state-of-the-art binary diffing tool BinDiff failed to find.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.277.2-277.2
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• 2013

Petri dishes and glass slides have been widely used as general substrates for in vitro mammalian cell cultures due to their culture viability, optical transparency, experimental convenience, and relatively low cost. Despite the aforementioned benefit, however, the flat two-dimensional substrates exhibit limited capability in terms of realistically mimicking cellular polarization, intercellular interaction, and differentiation in the non-physiological culture environment. Here, we report a protocol of culturing embryonic rat hippocampal neurons on the electro-spun polymeric network and the results from examination of neuronal cell behavior and network formation on this culture platform. A combinatorial method of laser-scanning confocal fluorescence microscopy and live-cell imaging technique was employed to track axonal outgrowth and synaptic connectivity of the neuronal cells deposited on this model culture environment. The present microfiber-based scaffold supports the prolonged viability of three-dimensionally-formed neuronal networks and their microscopic geometric parameters (i.e., microfiber diameter) strongly influence the axonal outgrowth and synaptic connection pattern. These results implies that electro-spun fiber scaffolds with fine control over surface chemistry and nano/microscopic geometry may be used as an economic and general platform for three-dimensional mammalian culture systems, particularly, neuronal lineage and other network forming cell lines.

• The Korean Journal of Archival Studies
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• no.52
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• pp.39-58
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• 2017

It is the responsibility of the government organization in the field of history and archives to control preproduction of records and their production sphere. Moreover, it should also manage all kinds of archives and presidential records as its function is to manage and share public information, and carry out compilations of historical records. With this, this study explains how having all these functions would make the ideal reorganization of the government. It should correspond to principle of Government's reorganization such as transparency, responsibility, communication. A plan for reformation needs two-track approach. I would like to propose the establishment of the 'Ministry of National Archives' or the 'National Memory Committee' at the organization that is in charge of national records and memory management. It means that the National memory isn't limited to public sphere. In terms of Total archives, the organizations should contain the whole community's memory. This organization should be formed independently.

• Korean Journal of Remote Sensing
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• v.15 no.2
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• pp.119-130
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• 1999

At present, many ground stations all over the world are using NORAD orbit element data in order to track and communicate with Earth orbiting satellites. The North American Aerospace Defense Command (NORAD) observes thousands of Earth orbiting objects on daily basis and provides their orbital information via internet. The orbital data provided by NORAD, which is also called two line element (TLE) sets, allows ground stations to predict the time-varying positions of satellites accurately enough to communicate with the satellites. In order to complete the mission of a high resolution remote sensing satellite which requires very high positional determination and control accuracy, however, a mission control and tracking ground station is dedicated for the observation and positional determination of the satellite rather than using NORAD orbital sets. In the case of KITSAT-3, NORAD orbital elements are currently used for image acquisition planning and for the processing of acquired images due to the absence of a dedicated KITSAT-3 tracking ground system. In this paper, we tested and analyzed the accuracy of NORAD orbital elements and the appropriate prediction model to determine how accurately a satellite acquisites an image of the location of interest and how accurately a ground processing system can generate the catalog of the images.

• Journal of Advanced Navigation Technology
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• v.25 no.3
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• pp.248-255
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• 2021

The main causes of the July 2013 OZ 214 accident were poorly performed approach and the failure to recognize the autothrottle in the HOLD position which the automated speed control was not provided. The pilots late decision for go-around was also a critical factor leading to the accident. The B777 POM restricts the use of FLCH mode beyond the FAF. This research utilized the QAR data of an airline's B777 fleet in the period of two years where 44 cases were found. In many cases, the FLCH mode was used for rapid descent from an higher than normal situation. In addition, in the base turn, continuous use of FLCH mode even when the path was below the glide path were observed. Airports with elevation above 500 ft MSL had a higher rate of occurrence. In this research, the proper descent planning and vertical path monitoring, and the adherence to the limitation set in the manuals and the stabilized approach criteria were re-emphasized as mitigation to reduce event occurences.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.38 no.11
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• pp.1309-1315
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• 2014

The switch for a maglev vehicle should be designed such that the vehicle safely changes its track without touching the guiderail. In particular, a medium-to-low-speed EMS -type maglev train relies heavily on a U-type electromagnet where it generates levitation force and guidance force simultaneously. Therefore, it is necessary to evaluate the safety of the vehicle whenever it passes the switch, as it lacks active control of the guidance force. Furthermore, when the vehicle passes a segmented switch, which is a group of curves made up of connected lines with a small radius of curvature, it may come into mechanical contact with the guiderail owing to the excessive lateral displacement of the electromagnet. The goal of this study is to analyze the influence of a segmented switch on the safety of major design-related variables for achieving improved running safety. We propose a three-dimensional multibody dynamics model composed of two cars with one body. Using the proposed model, we perform a simulation of the lateral air gap, which is one of the measurements of the running safety of the vehicle when it passes the switch. The analyzed design variables are the length between short span girder, the articulation angle, the length between two centers of a fixed girder at its ends, and the number of girders. On the basis of the effects of the considered design variables, we establish an optimized design of a switch with improved safety.

• Microbiology and Biotechnology Letters
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• v.39 no.1
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• pp.29-36
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• 2011

Pseudomonas aeruginosa is an opportunistic pathogen that causes various infections on urinary track, cornea, respiratory track, and burn wound site, and mainly relies on quorum sensing (QS) for its virulence. To control the infectivity of P. aeruginosa, we previously synthesized the structural analogues of a major QS signal, N-3-oxododecanoyl homoserine lactone (3OC12-HSL) to use as a QS inhibitor. Two of them (5b and 5f) had been confirmed to have an inhibitory effect on LasR, a major QS signal receptor of P. aeruginosa in the screening by the recombinant Escherichia coli reporter. To further evaluate these compounds, we tested their efficacy to control the QS and virulence of P. aeruginosa. Unlike the result from E. coli reporter, both 5b and 5f failed to affect the LasR activity in P. aeruginosa, but instead they selectively affected the activity of QscR, another 3OC12-HSL receptor of P. aeruginosa. Interestingly, their effect on QscR was complex and opposite to what we obtained with E. coli system. Both 5b and 5f enhanced the QscR activity at the low concentration range (< 10 ${\mu}m$), but high concentration of 5f (${\approx}$1 mM) strongly inhibited QscR. While 5b and 5f didn't affect the production of proteases, the key virulence factor, they significantly reduced the biofilm formation that is important in mediating chronic infections. Especially, 5f inhibited the initial attachment of P. aeruginosa, rather than the biofilm maturation. Based on our results, we suggest that 5f can be applied for an anti-biofilm agent without increasing virulence of P. aeruginosa.