Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Cytotoxicity of Diesel Exhaust Particles from Various Vehicles toward Macrophage Cells

국내 디젤 차량 배기 입자가 쥐 대식세포에 미치는 세포독성 평가

  • Lee, Jang-Han (College of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Yong-Kwon (Department of Food and Nutrition, Yuhan University) ;
  • Lee, Ji-Young (College of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Seung-Bok (Global Environment Center, Korea Institute of Science and Technology) ;
  • Kim, Sun-Hwa (Global Environment Center, Korea Institute of Science and Technology) ;
  • Bae, Gwi-Nam (Global Environment Center, Korea Institute of Science and Technology) ;
  • Lee, Hak-Sung (Civil and Information System, Seowon University) ;
  • Lim, Cheol-Soo (National Transportation Pollution Research Center, National Institute of Environmental Research) ;
  • Chung, Nam-Hyun (College of Life Sciences and Biotechnology, Korea University)
  • 이장한 (고려대학교 생명공학과) ;
  • 이용권 (유한대학 식품영양과) ;
  • 이지영 (고려대학교 생명공학과) ;
  • 이승복 (한국과학기술연구원 지구환경센터) ;
  • 김선화 (한국과학기술연구원 지구환경센터) ;
  • 배귀남 (한국과학기술연구원 지구환경센터) ;
  • 이학성 (서원대학교 환경건설정보학과) ;
  • 임철수 (국립환경과학원 교통환경연구소) ;
  • 정남현 (고려대학교 생명공학과)
  • Received : 2010.01.07
  • Accepted : 2010.06.28
  • Published : 2010.06.30
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Abstract

DEPs (diesel exhaust particles) like any other particles can be also inhaled into lung to participate in a damaging reaction to the organ. Possible damages might be apoptosis and inflammatory responses to the cells in respiratory track. The aim of this study was cytotoxicity evaluation of DEPs from five in-use diesel vehicles using a murine macrophage cell (RAW 254.7). We found that most DEPs have a considerable cytotoxicity compared to the control and SRM 2975. When measured by MTT assay and extents of apoptosis, DEPs of two highmileage vehicles had higher toxicity than those of the other three low-mileage vehicles tested. Although mRNA expression level of TNF-${\alpha$ somewhat explains the trend of cytotoxicity and apoptosis, that of IL-1$\beta$ did not. Correlation studies among the extents of MTT assay, apoptosis, and TNF-$\alpha$ expression showed that the extents between apoptosis and TNF-$\alpha$ expression was most highly correlated (r=0.96). These results suggest that cytotoxicity of various DEPs could be compared easily by measuring the extent of apoptosis or TNF-$\alpha$ expression by DEPs.

Keywords

References

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