• Korean Journal of Environmental Agriculture
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• v.37 no.4
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• pp.277-282
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• 2018

BACKGROUND: At these days, the human health and environmental concerns of pesticide used for turf grass management at golf courses in Korea have increased. The objectives of the study were to determine the pesticide residues for golf course and neighboring area and evaluate the impact moved into neighboring area of pesticides treated at golf courses. METHODS AND RESULTS: Three golf courses and neighboring areas in Korea were monitored from July to October, 2017. The soil sample collection was divided the golf course into its logical parts (such as a greens, fairways, and rough) and neighboring area soil samples were collected at three different points. The water samples of the golf course and neighboring area were collected at three different points, respectively. The pesticide residues for soil and water sample were monitored by the multi-residue screening method of 98 pesticide with HPLC-MS-MS. The concentrations of detected pesticide in soil and water samples of the golf course were in the range of 0.01~1.26 mg/kg and 0.0001~0.0089 mg/kg, respectively. The residue levels for detected pesticides in neighboring area were at 0.01~0.04 mg/kg and 0.0001~0.0029 mg/kg, respectively, well below those level in golf course. CONCLUSION: This study indicate that the pesticide residue levels of golf course and neighboring area in Korea may not a possible risk of exposure on soil and aquatic environment. For future work, more monitoring should be performed so that the evaluation data becomes more valid.

• Asian Journal of Turfgrass Science
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• v.24 no.2
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• pp.211-217
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• 2010

This study was conducted to investigate a proper method of ball mark repair by comparing the creeping bentgrass recovery speed on golf course green treated by various methods of ball mark repair. Nine general repairing methods were tested and compared; control (no repair, A type), two common methods of USGA (B type) and GCSAA (C type), three methods with fork shaped hand set performing at Korean golf courses (Ansung Benest, D; Sky72, E; Lakeside, F type), and three methods using the repair machine with 6, 8, or 14 teeth (G, H, I type, respectively). Three creeping bentgrass cultivar of 'Penncross', 'T-1', and 'CY-2' were tested in this field experiment. This test was carried out from September to November in 2009 at the nursery on the Seoul Lakeside Golf course. The average speed of turfgrass recovery after various ball mark repairing methods have been ranked as in the order of E, D, C, B, F, I, H, G, and A. The methods of hand practise showed more effective results than repair method using machines. The ball mark recovery speeds of 'Penncross' were in the order of E, D, B, C, F, I, H, and A. In the case of 'T1' and 'CY-2', similar orders were showed as D, E, B, F, C, H, I, A, G and the order of D, E, C, F, B, H, G, I, A, respectively. The ball mark recovery speed among creeping bentgrass cultivar resulted in the order of 'CY-2', 'Penncross', and 'T-1'. The most proper method of ball mark repair was repair method using a hand set tool especially the method of the Sky72 Golf course (E type). At the first, remove a damaged grass area with fork and tap. And then gather the side grasses into the center area with pulling the grasses with fork. After that, make harden and flat on the turf surface by pounding and rolling with the round wooden stick. The final Nstep, water the repaired grass surface. This ball mark repairing practise showed a most rapid and proper recovery method on creeping bentgrass green.

• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.229-240
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• 2009

The high temperature and water content in soil profile probably affect the physiological disorder especially on cool-season turfgrasses in warm and humid weather of Korean summer. The purpose of this research was to analyze the effect of soil temperature and water content on the growth and stress response of creeping bentgrass(Agrostis palustris Huds.) under a humid and warm temperature. USGA(United State of Golf Association) green profile in laboratory test, Daily temperature changes were tested under a dried sand, 70% water content of field capacity, and saturated condition at $34^{\circ}C$ of the USGA green in lab. In this test, the dried sand reached to $80^{\circ}C$, however, the surface temperature decrease of $10^{\circ}C$ on the saturated condition. In the thermal properties test in field, thermal conductivity, thermal diffusivity, and soil temperature were increased followed by irrigation practise. In the water-deficient condition, the highest soil temperature was reached temporally right after irrigation, however, the excessive soil water content higher than field water holding capacity showed the highest soil temperature after a while. This result indicated that a heat damage to root system was caused from the thermal conductivity of a high surface soil temperature. The excessive irrigation when a high turf surface temperature should occur a negative result on tufgrass growth, moreover, it would be fatal to root growth of creeping bentgrass, especially when associated with a poor draining system on USGA sand green. Overall, this study shows that high soil temperature with water-excessive condition negatively affects on cool-season grass during the summer season, suggesting that excessive irrigation, over 70% field capacity of soil condition, does not help to reduce soil temperature for summer season in Korea. In the study that cool-season grass were treated with different water content of soil, The soil had higher temperature and more water holding capacity when treatment rate of soil conditioner was increased. The best growth at the normal water condition and the worst state of growth at thee water-excessive condition were observed.

• Journal of Plant Biotechnology
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• v.35 no.4
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• pp.245-256
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• 2008

The current legislation in South Korea clearly states that the tolerance threshold on the adventitious presence of GMO in environment-friendly agricultural products is 3.0% and no GM seed should be detected in their planting seed batches. To date, in Korea, there is no approved GM crop for commercial cultivation in field. However, several GM crops including rice, Chinese cabbage, potato and wild turf grass are currently under risk assessment for their environmental release. Also Korean government (Rural Development Administration, RDA) announced that 11 institutes including universities have been currently certified to carry out a risk assessment of GM crops. Meanwhile, the cultivated area and certified quantities of environment-friendly crops (organic, pesticide-free and low-pesticide) are sharply increasing every year according to the report of National Agricultural Products Quality Management Service (NAQS). In detail, in 2007, the certified quantities of environment-friendly agricultural products were elevated up to 100-fold for organic, 171-fold for pesticide-free and 2,324-fold for low-pesticide crops when compared with those in 1999. The total certified quantity of environment-friendly cereal crops in 2007 was equivalent to 6.4% of total production of cereal crops. Moreover, 24% of total production of root and tuber crops such as potato and sweet potato were certified for environment-friendly agricultural products. In these circumstances, I strongly suggest that current legislations on GM crop's safety management should be revised to include strategies for the coexistence of GM with non-GM crops, especially environment-friendly crops before GM crop is approved to be cultivated for commercialization. Since all types of crops are grown in an open environment, the adventitious presence of GM crops among non-GM crops is inevitable if appropriate measures for coexistence are not established for species by species such as isolation distance, workable management measures to minimize admixture.

• Asian Journal of Turfgrass Science
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• v.18 no.3
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• pp.149-163
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• 2004

Physical and chemical properties of root zone mixes and methods of green construction are important considerations for improving turf grass quality for putting greens. This study compared Penncross creeping bentgrass (Agrostis palustris Huds.) performance as affected by three root zone construction systems with three amendments (sand, peat, and zeolite). The objective of this study was to determine if an amended California construction system would improve green performance during establishment (1998-1999) and maturation (2000-2001). Three treatments were tested: California ($100\%$ sand), USGA($90\%$ sand and $10\%$ peat, v/v), and California-Z ($85\%$ sand and $15\%$ zeolite, v/v). Treatments were arranged in a randomized complete block with four replicates. Physical and chemical properties of the root zone and bentgrass performance were compared for the treatments. The California-Z treatment had the highest saturated hydraulic conductivity, field infiltration rate and the lowest bulk density. It also had the highest cation exchange capacity and plant available nutrient concentrations among the three treatments. The California-Z treatment produced bentgrass quality and color during green establishment and maturation that were equal to or higher than the California treatment, and consistently higher than the USGA treatment. The addition of an inorganic amendment to the California system improved physical and chemical properties of the root zone and improved quality and color of bentgrass during green establishment. During green maturation, creeping bentgrass in the California-Z treatment was equal (6 of 15 sampling dates) or $20\%$ higher (9 of 15 dates) in quality compared to the California system.

• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.133-142
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• 2009

This study was initiated to evaluate the K leaching potential in the green soils and K uptake by the turfgrass in the golf course using the K fertilizers. The turfgrass, Floradwarf bermudagrass(Cynodon dactylon L. $P_{ERS}$.) was planted and grown in the mixture of sand and peat moss in this lysimeter study. Eight representative K fertilizers, such as, monopotassium phosphate (MKP), KCL, $K_2SO_4$, $KNO_3$, CKCl, $CK_2SO_4$, $CKNO_3$, and 0-20-20(liquid) were used in this study. Based on the total K quantity of leachate collected during the whole 12 weeks, 0-20-20 is the K fertilizers the most contributing to the leaching of K, then MKP, the second, KCL, the third, and finally $KNO_3$ are K fertilizers contributing to the K leaching. However, most amount of K applied and collected in the lysimeter were leached during the first period of two and four weeks, compared to that of K leached during the second period of six, eight, ten, and twelve weeks. Application of CKCL and $CK_2SO_4$ producted the largest amount of total dry matter, then MKP and KCL, $KNO_3$ and $CKNO_3$, 0-20-20 in second group. However, except $K_2SO_4$, most K fertilizer sources such as MKP, KCL, $KNO_3$, CKCL, $CK_2SO_4$, $CKNO_3$, 0-20-20 showed the largest amount of K uptake, except $K_2SO_4$. Therefore, based on the K leaching, dry matter production, and plant K uptake, it appears that the coated fertilizers, CKCL, $CKNO_3$, and $CK_2SO_4$ are the environmentally sound fertilizers recommended in the turfgrass green soil of golf course.

• Horticultural Science & Technology
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• v.35 no.3
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• pp.344-360
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• 2017

Zoysiagrasses are important turf plants used for school playgrounds, parks, golf courses, and sports fields. The two most popular zoysiagrass species are Zoysia japonica and Zoysia sinica. These are widely distributed across different growing zones and are morphologically distinguishable from each other; however, it is phenotypically difficult to differentiate those that grow along the coastal line from those in beach area habitats. A combination of morphological and molecular approaches is desirable to efficiently identify these two plant cultivars. In this study, we used a rapid identification system based on DNA barcoding of the nrDNA-internal transcribed spacer (ITS) regions. The nrDNA-ITS regions of ITS1, 5.8S nrDNA, and ITS2 from Z. japonica, Z. sinica, Agrostis stolonifera, and Poa pratensis were DNA barcoded to classify these grasses according to their molecular identities. The nrDNA-ITS sequences of these species were found at 686 bp, 687 bp, 683 bp, and 681 bp, respectively. The size of ITS1 ranged from 248 to 249 bp, while ITS2 ranged from 270 to 274 bp. The 5.8S coding region ranged from 163 - 164bp. Between Z. japonica and Z. sinica, nineteen (2.8%) nucleotide sites were variable, and the G+C content of the ITS region ranged from 55.4 to 63.3%. Substitutions and insert/deletion (indel) sites in the nrDNA-ITS sequence of Z. japonica and Z. sinica were converted to cleaved amplified polymorphic sequence (CAPS) markers, and applied to the Zoysia grasses sampled to verify the presence of these markers. Among the 62 control and collected grass samples, we classified three groups: 36 Z. japonica, 22 Z. sinica, and 4 Z. japonica/Z. sinica hybrids. Morphological classification revealed only two groups; Z. japonica and Z. sinica. Our results suggest that used of the nrDNA-ITS barcode region and CAPS markers can be used to distinguish between Z. japonica and Z. sinica at the species level.

• Asian Journal of Turfgrass Science
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• v.22 no.2
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• pp.217-223
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• 2008

This study was conducted to figure out the effect of silicate as growth stimulator on growth of 'Pencrosss' creeping bentgrass. Creeping bentgrass(Agrostis palustris cv. 'Pencross') at the nursery of Sinwon Country Club was used. Silicate was applied at the concentration of 0, $200{\times}$, $500{\times}$, $1.000{\times}$. Polt size was 1 by 2 meter and there were three replications with completely randomized design(CRD). Creeping bentgrass growth was evaluated with visual turf grass quality, root length and No. of tiller density(ea/$cm^2$). Results of this study are as followings; 1. Average root length with silicate was $1.5{\sim}1.9cm$ longer than control. Especially, Root length of silicate was $7{\sim}8cm$ in summer. 2. Tiller density with silicate was $l8{\sim}22ea/cm^2$, $0.4{\sim}2.l$ less than control. But there was no significant difference. 3. Visual turfgrass quality with silicate was $5.0{\sim}8.3$, $0.3{\sim}1.5$ higher than control. But there was no significant difference. In conclusion, silicate might be grown as root growth stimulator of creeping bentgrass during summer in Korea. However, this study was conducted under one year. Accordingly, in-depth experiment should be done over several years.

• Asian Journal of Turfgrass Science
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• v.24 no.1
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• pp.45-49
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• 2010

Research on nitrate-nitrogen ($NO_3-N$) leaching in turfgrass indicates that in most cases leaching poses minimal risk to the environment. Although there have been many studies investigating $NO_3-N$ leaching, there has been little research to investigate the effect of compaction level and rootzone mix on nitrogen (N) leaching. The research objective is to determine the effect of compaction level and rootzone mix on nitrogen leaching. The four rootzone mixes are 76.0:24.0, 80.8:19.2, 87.0:13.0 and 93.7:6.3 % (sand:soil). The four levels of compaction energies are 1.6, 3.0, 6.1, and 9.1 J $cm^{-2}$. Nitrogen was applied using urea at a rate of 147 kg $ha^{-1}$ split among three applications. Rootzone was packed into a polyvinylchloride pipe with a perforated bottom to facilitate drainage. Rootzone depth was 30 cm over a 5 cm gravel layer. Each column was sodded with Poa pratensis L. Hoagland solution designed for coolseason grasses, minus N, was used to ensure adequate nutrition in the rootzone. Turf grass quality and clipping yield were recorded from each tube at two-week intervals. The clippings were oven-dried at a temperature of $67^{\circ}C$ for 24 h and weighed. At the end of the study, root dry weight was determined by washing and oven-drying samples at $67^{\circ}C$ for 24 h. Leachate solution was collected weekly for analysis. More than 6.1 J $cm^{-2}$ of compaction energy increased possibilities of surface runoff. The compaction energy between 3.0 and 6.1 J $cm^{-2}$ produced more clipping dry weight and less N leaching than 9.1 J $cm^{-2}$.

• Korean Journal of Weed Science
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• v.16 no.3
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• pp.221-229
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• 1996

This study was carried out to determine the effects of growth regulators, carbon sources and silver nitrate on callus formation and plant regeneration of turfgrass. The results were summarized as fallows : Callus from Korean lawngrass (Zoysia japonica Steud.) and pencross creeping bentgrass (Agrostis palustrir Huds.) induced better in MS medium than in N6 medium and by addition of 2,4-D than by that of NAA. Callus formation from Korean lawngrass and penncross creeping bentgrass was very effective at MS medium adding 1mg/L 2,4-D and at the medium adding 2mg/L 2,4-D, repectively. Growth of callus was good at MS medium containing 2mg/L 2,4-D+0.2mg/L NAA. Callus growth of Korean lawngrass and penncross creeping bentgrass was good when kinetin was added 0.2mg/L and 0.3mg/L, individually, to MS medium containg 2mg/L 2,4-D+0.2mg/L NAA. Regeneration rate from leaf and stock callus of Korean lawngrass was 44% at MS medium adding 2,4-D 2mg/L+NAA 0.2mg/L+kinetin 0.3mg/L and 32% at the medium containing 2,4-D 2mg/L+NAA 0.2mg/L+kinetin 0.3mg/L, each and that from leaf and stock callus of penncross creeping bentgrass was 80% and 67%, each, at the medium adding 2,4-D 2mg/l+NAA 0.2mg/L+kinetin 0.3mg/L. Regeneration rate of Korean lawngrass and penneross creeping bentgrass increased by 3 to 4% and by 10 to 16%, respectively, when added $AgNO_3$ 1~2mg/L to the above-mentioned regeneration medium.