• 한국자기공명학회논문지
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• 제16권2호
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• pp.172-184
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• 2012

The prokaryotic molecular chaperone Hsp33 achieves its holdase activity upon response to oxidative stress particularly at elevated temperature. Despite many structural studies of Hsp33, which were conducted mainly by X-ray crystallography, the actual structures of the Hsp33 in solution remains controversial. Thus, we have initiated NMR study of the reduced, inactive Hsp33 monomer and backbone NMR assignments were obtained in the present study. Based on a series of triple resonance spectra measured on a triply isotope-[$^2H/^{13}C/^{15}N$]-labeled protein, sequence-specific assignments of the backbone amide signals observed in the 2D-[$^1H/^{15}N$]TROSY spectrum could be completed up to more than 96%. However, even considering the small portion of non-assigned resonances due to the lack of sequential connectivity, we confirmed that the total number of observed signals was quite smaller than that expected from the number of amino acid residues in Hsp33. Thus, it is postulated that peculiar dynamic properties would be involved in the solution structure of the inactive Hsp33 monomer. We expect that the present assignment data would eventually provide the most fundamental and important data for the progressing studies on the 3-dimensional structure and molecular dynamics of Hsp33, which are critical for understanding its activation process.

• 한국자기공명학회논문지
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• 제25권3호
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• pp.39-44
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• 2021

The CRISPR-Cas system provides adaptive immunity for bacteria and archaea against invading phages and foreign plasmids. In the Class 1 CRISPR-Cas system, multi-subunit Cas proteins assemble with crRNA to bind to DNA targets. To disarm the bacterial defense system, bacteriophages evolved anti-CRISPR (Acr) proteins that actively inhibit the host CRISPR-Cas function. Here we report the backbone resonance assignments of AcrIF7 protein that inhibits the type I-F CRISPR-Cas system of Pseudomonas aeruginosa using triple-resonance nuclear magnetic resonance spectroscopy. We employed various computational methods to predict the structure and binding interface of AcrIF7, and assessed the model with experimental data. AcrIF7 binds to Cas8f protein via flexible loop regions to inhibit target DNA binding, suggesting that conformational heterogeneity is important for the Cas-Acr interaction.

• 한국자기공명학회논문지
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• 제27권2호
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• pp.5-9
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• 2023

t ENOD40B, a plant peptide hormone, was doubly labeled with C-13 and N-15 by recombinant production in Escherichia coli. The peptide was prepared by affinity chromatography followed by protease cleavage and reverse-phase chromatography. To elucidate the mode of action against its receptor, sucrose synthase, we proceeded to assign the backbone and side-chain resonances using a set of double and triple resonance experiments. This result will be used to determine the three-dimensional structure of the peptide at its bound state as well as to observe the chemical shift changes upon binding.

• 한국전자파학회논문지
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• 제22권4호
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• pp.447-452
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• 2011

본 논문에서는 새로운 구조의 작은 크기의 삼중 모드 공진기를 제안한다. 제안된 구조는 소형화를 위해 반파장의 Stepped Impedance Resonaotr에 개방 스터브와 단락 스터브를 이용하였으며, 개방 스터브에 의해 자체적으로 하나의 전송 영점을 발생시킨다. 각각의 세 가지 공진 모드에서 발생하는 공진점을 이론적으로 해석하였다. 제안된 삼중 모드 공진기를 이용하여 세 개의 전송 극점과 하나의 전송 영점을 갖는 2.4 GHz WLAN에 적용 가능한 대역 통과 필터를 설계 및 제작하였다. 제작된 삼중 모드 필터는 중심 주파수 2.4 GHz에서 15.8 %의 3 dB 대역폭과 2.4~2.5 GHz 통과 대역 내에서 최대 1.17 dB 이하의 삽입 손실을 갖는다. 제작된 삼중 모드 공진기의 필터의 크기는 급전 선로를 포함하여 15.9 mm${\times}$9.7 mm로 기존의 삼중 모드 필터에 대비하여 매우 작은 크기를 갖는다.

• 대한자기공명의과학회:학술대회논문집
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• 대한자기공명의과학회 2001년도 제6차 학술대회 초록집
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• pp.108-108
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• 2001

Purpose： To correlate the histological differentiation of hepatocellular carcinomas (HCCs) with finding on triple contrast-enhanced MR imaging using gadolinium-chelates, superparamganetic ire oxides (SPIO), and mangafodipir trisodium. Method： Ten patients with proven HCC underwent triple contrast-enhanced MRI befo surgical resection. Subjective ratings of the enhancement pattern and degree were compare with the histological grades determined on surgical specimen. Quantitative measurements signal-to-noise ratio (S/N) of the lesion and the lesion-to-liver contrast-to-noise ratio C/N on the enhanced MR images, and the degree of S/N and C/N changes between the unenhanced and enhanced images were also correlated with the histological grades.

• 한국자기공명학회논문지
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• 제10권2호
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• pp.155-162
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• 2006

Angiogenin is unique among angiogenic molecules in that it is a member of the pancreatic ribonuclease superfamily and, in fact, is a ribonucleolytic enzyme. Its enzymatic activity is extremely weak compared to that of the digestive RNases but is critical for its capacity to induce neovascularization. In this study, we completed the backbone resonance assignment of bovine angiogenin using triple resonance NMR experiments of $^{15}N\;and/or\;^{13}C$ isotope labeled protein and investigated the chemical shift variation upon binding with inhibitor phosphate ion and determine the phosphate binding site.

• JSTS:Journal of Semiconductor Technology and Science
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• 제11권4호
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• pp.295-301
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• 2011

A triple-band transceiver module for 2.3/2.5/3.5 GHz mobile WiMAX, IEEE 802.16e, applications is introduced. The suggested transceiver module consists of RFIC, reconfigurable/multi-resonance MIMO antenna, embedded PCB, mobile WiMAX base band, memory and channel selection front-end module. The RFIC is fabricated in $0.13{\mu}m$ RF CMOS process and has 3.5 dB noise figure(NF) of receiver and 1 dBm maximum power of transmitter with 68-pin QFN package, $8{\times}8\;mm^2$ area. The area reduction of transceiver module is achieved by using embedded PCB which decreases area by 9% of the area of transceiver module with normal PCB. The developed triple-band mobile WiMAX transceiver module is tested by performing radio conformance test(RCT) and measuring carrier to interference plus noise ratio (CINR) and received signal strength indication (RSSI) in each 2.3/2.5/3.5 GHz frequency.

• 한국자기공명학회논문지
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• 제22권3호
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• pp.71-75
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• 2018

The CRISPR-Cas system is the adaptive immune system in bacteria and archaea against invading phages or foreign plasmids. In the type II CRISPR-Cas system, an endonuclease Cas9 cleaves DNA targets of phages as directed by guide RNA comprising crRNA and tracrRNA. To avoid targeting and destruction by Cas9, phages employ anti-CRISPR (Acr) proteins that act against host bacterial immunity by inactivating the CRISPR-Cas system. Here we report the backbone $^1H$, $^{15}N$, and $^{13}C$ resonance assignments of AcrIIA4 that inhibits endonuclease activity of type II-A Listeria monocytogenes Cas9 and also Streptococcus pyogenesis Cas9 using triple resonance nuclear magnetic resonance spectroscopy. The secondary structures of AcrIIA4 predicted by the backbone chemical shifts show an ${\alpha}{\beta}{\beta}{\beta}{\alpha}{\alpha}$ fold, which is used to determine the solution structure.

• 한국전자파학회논문지
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• 제17권2호
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• pp.178-183
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• 2006

본 논문에서는 결합 선로를 적용한 변형된 미엔더 라인과 기본적인 형태의 미엔더 라인을 커플링 결합을 통하여 적층함으로써 모바일 RFID, PCS 그리고 WiBro의 삼중 공진 대역 특성을 나타내는 소형 칩 안테나를 제안하였다. 결합 선로의 길이와 미엔더 선로의 간격을 제어하여 모바일 RFID, PCS 대역을 확보한 후 기본 형태의 미엔더 선로를 적층하여 WiBro 대역을 구하였다. 제안된 안테나는 LTCC 공정을 통하여 $10.98{\times}22.3{\times}0.98\;mm$의 크기로 제작되었다. 공진 주파수는 각각 905 MHz, 1.77 GHz 그리고 2.32 GHz에서 공진을 하고 있는 것을 확인하였다. 임피던스 대역폭($VSWR{\le}2$)이 24 MHz, 140 MHz 그리고 92 MHz를 나타내었다. 각 공진 주파수에서의 최대 이득은 0.34 dBi, 2.58 dBi 그리고 0.4 dBi를 나타내었다.

• 한국항행학회논문지
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• 제16권3호
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• pp.433-439
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• 2012

본 논문에서는 CRLH (Composite Right/Left-Handed) 전송선로의 단위 셀이 갖고 있는 대역통과 특성을 이용하여 서로 다른 대역의 간섭을 최소화한 트리플렉서를 제안하였다. 트리플렉서의 채널 필터는 서로 다른 채널의 공진주파수에서 개방된 것과 같은 입력 임피던스를 갖고 있는데, 이러한 특성은 CRLH 전송선로의 단위 셀의 직렬 공진과 병렬 공진의 결합에 의한 것이다. 구현된 트리플렉서의 측정 결과는 삽입손실 1.5 dB 이하, 반사손실 15 dB 이하, 각 포트간 격리도 25 dB 이하가 되었다. 이러한 특성을 갖는 트리플렉는 삼중대역 증폭기의 입출력부에 연결하여 설계, 실험하였고, 그 결과는 예측된 결과와 유사한 측정결과를 얻었다.