This study was carried out to determine whether genistein implants in laying hens could be transferred into their eggs. 250 mg genistein pellet was implanted two or four subcutaneously in the neck of laying hens. The contents of genistein in egg yolk transferred were analyzed with HPLC-MS. In 500 mg genistein pellets, it was detected as 395 ng/egg yolk on the day 18 after implanting and maintained as 546 ng/egg yolk after the day 59. In 1,000 mg, genistein was detected as 240 ng/egg yolk on the day 13, as 514 ng/egg yolk on the day 30 and maintained over 59 days. In conclusion, the direct genistein implants could be more twenty times efficiently transferred to egg yolks than dietary supplement.
A genetic transfer system for introducing foreign genes to biomining microorganisms is urgently needed. Thus, a conjugative gene transfer system was investigated for a moderately thermophilic, extremely acidophilic biomining bacterium, Acidithiobacillus caldus MTH-04. The broad-hostrange IncP plasmids RP4 and R68.45 were transferred directly into A. caldus MTH-04 from Escherichia coli by conjugation at relatively high frequencies. Additionally the broad-hostrange IncQ plasmids pJRD215, pVLT33, and pVLT35 were also transferred into A. caldus MTH-04 with the help of plasmid RP4 or strains with plasmid RP4 integrated into their chromosome, such as E. coli SM10. The $Km^r\;and\;Sm^r$ selectable markers from these plasmids were successfully expressed in A. caldus MTH-04. Futhermore, the IncP and IncQ plasmids were transferred back into E. coli cells from A. caldus MTH-04, thereby confirming the initial transfer of these plasmids from E. coli to A. caldus MTH-04. All the IncP and IncQ plasmids studied were stable in A. caldus MTH-04. Consequently, this development of a conjugational system for A. caldus MTH-04 will greatly facilitate its genetic study.
This study investigated the contribution of Korean phonological awareness to English phonological awareness and the relations of phonological awareness with reading and writing in Korean Hangul and English among Korean 5th graders. With age and vocabulary knowledge statistically controlled, Korean phonological awareness was transferred to English phonological awareness. Specifically, syllable and phoneme awareness in Korean transferred to syllable awareness in English, and Korean phoneme awareness transferred to English phoneme awareness. In addition, English phoneme awareness independently explained significant variance of reading and writing in Korean and English after controlling for age and vocabulary. Syllable awareness in Korean and English explained Hangul reading and writing, respectively. The results suggest cross-language transfer of phonological awareness that is a metalinguistic skill. Phoneme awareness is important in reading and writing in English whereas both of syllable and phoneme awareness are important in literacy of Korean.
This study is for implementation of PC based Motor fault diagnosis system. By using harmonics and current signals of the motor, this system diagnoses the motor condition by accumulated harmonic contribution rate. In this proposed system that was composed of 5 parts. A sensor, connection box, evaluation board, device server, and main computer are those. There were two types of sensor, one was harmonic sensor the other was current sensors. The signal was acquired by sensor, and transferred to evaluation board. Second one is connection box. Because the output type of sensor and input type of evaluation board is different, connection box was necessary. Third one was evaluation board. The signal from the sensor was converted to digital signal in evaluation board. And this signal was transferred to device server. Fourth one was device server. Device server transferred the data from evaluation board to main computer. And the last one was other parts controlled by main computer. In main computer, there were communication and diagnosis algorithms. The result was derived by main computer. In the result, there were 12 categories and 5 levels of motor conditions. The proposed system had some advantages comparing with stand alone type commercial motor fault diagnosis system. The first, by using remote access it was easier to get the conditions of motor. The second, there was no need to handle the sensors when users measured the motor signals. By this property, no one was necessary at motor location site. The third, this system was less restricted by times and places than commercial stand alone type diagnosis system. Therefore users can operate this system only using the main computer. Once the sensors are installed at the motor, users doesn't need to move to check up the condition of motors. Moreover, if there is ethernet hub, many motors can be not only diagnosed at once but also decreased its cost.
This study is for implementation of PC based Motor fault diagnosis system. By using harmonics and current signals of the motor, this system diagnoses the motor condition by accumulated harmonic contribution rate. In this proposed system that was composed of 5 parts. A sensor, connection box, evaluation board, device server, and main computer are those. There were two types of sensor, one was harmonic sensor the other was current sensors. The signal was acquired by sensor, and transferred to evaluation board. Second one is connection box. Because the output type of sensor and input type of evaluation board is different, connection box was necessary. Third one was evaluation board. The signal from the sensor was converted to digital signal in evaluation board. And this signal was transferred to device server Fourth one was device solver. Device server transferred the data from evaluation board to main computer. And the last one was other parts controlled by main computer. In main computer, there were communication and diagnosis algorithms. The result was derived by main computer. In the result, there were 12 categories and 5 levels of motor conditions. The proposed system had some advantages comparing with stand alone type commercial motor fault diagnosis system. The first, by using remote access it was easier to get the conditions of motor. The second, there was no need to handle the sensors when users measured the motor signals. By this Property, no one was necessary at motor location site. The third, this system was less restricted by times and places than commercial stand alone type diagnosis system. Therefore users can operate this system only using the main computer. Once the sensors are installed at the motor, users doesn't need to move to check up the condition of motors. Moreover, if there is ethernet hub, many motors can be not only diagnosed at once but also decreased its cost.
Presently, we report a simple, reproducible and high frequency plant regeneration in Hibiscus syriacus L. using axillary buds. H. syriacus was regenerated from axillary buds directly or through a callus phase. Regenerated shoots were directly induced from young and fresh axillary buds cultured on Murashige and Skoog medium (MS) supplemented with 0.01 mg/L of the growth regulator thidiazuron (TDZ) after 2 weeks of culture. Directly induced shoots were transferred to hormone-free MS medium and root development was observed after 6 weeks. On the other hand, old and stale axillary buds were regenerated to shoots via callus induction on MS medium containing 0.01–2 mg/L TDZ after 4 weeks. A TDZ concentration of 0.01 mg/L was most effective in callus formation. Green callus was transferred to MS medium containing 0.01 mg/L α-naphthalene acetic acid (NAA) and 0.5 mg/L benzylaminopurine (BA). After 4 weeks, callus had developed into multiple shoots. Plantlets were formed from 10 week cultures of single shoots on hormone-free MS medium. Regenerated plantlets were cultured on MS medium for one month and then transferred to pots containing garden soil. Potted plants were acclimatized for one month and grown to maturity under greenhouse conditions. The present study has shown that various concentrations of plant growth regulator can be effective for in vitro plant regeneration of H. syriacus. The direct and indirect regeneration protocol presented here will be useful for understanding the manipulation and propagation of H. syriacus.
The purpose of this research which was conducted by surveying the transfer consultation records from 360 medical institutions such as general hospitals, hospitals, clinics to the Emergency Medical Center at E University Hospital for six months(Jan. 1, 2000 - Jun. 30, 2000) are to standardize & complete transfer consultation record of hospitals at the 1st & 2nd referral level and to give patients transferred emergency medical center medical information services on a better quality. The conclusions and suggestions from this study were summarized as follows; (1) Examing the distribution of the referral medical consultation(transfer) sheet type, surgery part local clinic sheet types were 34.4%, medical part local clinic sheet types were 26.7%, undifferentiated local clinic sheet types were 23.9% and hospital level sheet types were 15.0%. (2) The items of the transfer consultation records had been standardized more than 75% in the order of patient's name, date, doctor's name, diagnosis, patient's status, impressions. (3) That the degree of recording completion on these items is in the order of patient's name, date, diagnosis, impressions was revealed. (4) Because the standardization and the degree of recording completion are very low in the patient's gender, age, address, electronic recording system was needed for more perfect input of initial patient informations. (5) This standardizing & complete recording on examination and medication will prevent re-examination and abuse of medication for patients transferred emergency medical center. (6) EMT Transfer System should be fixed in all medical institute for the standardizing & complete recording on care period and departure time will give many emergency patients the proper treatments at the proper time. (7) It was revealed that developing new standardized transfer consultation record & using electronic recording system are needed. (8) The complete recording & Fast Track System were needed for higher rate of bed operation at emergency medical center and more hospital profit.
Based on theories of rock mechanics, rock fragmentation, mechanics of elasto-plasticity, and energy dissipation etc., a method is presented for evaluating the rock fragmentation efficiency by using plastic energy dissipation ratio as an index. Using the presented method, the fragmentation efficiency of rocks with different strengths (corresponding to soft, intermediately hard and hard ones) under indentation is analyzed and compared. The theoretical and numerical simulation analyses are then combined with experimental results to systematically reveal the fragmentation mechanism of rocks under indentation of indenter. The results indicate that the fragmentation efficiency of rocks is higher when the plastic energy dissipation ratio is lower, and hence the drilling efficiency is higher. For the rocks with higher hardness and brittleness, the plastic energy dissipation ratio of the rocks at crush is lower. For rocks with lower hardness and brittleness (such as sandstone), most of the work done by the indenter to the rocks is transferred to the elastic and plastic energy of the rocks. However, most of such work is transferred to the elastic energy when the hardness and the brittleness of the rocks are higher. The plastic deformation is small and little energy is dissipated for brittle crush, and the elastic energy is mainly transferred to the kinetic energy of the rock fragment. The plastic energy ratio is proved to produce more accurate assessment on the fragmentation efficiency of rocks, and the presented method can provide a theoretical basis for the optimization of drill bit and selection of well drilling as well as for the selection of the rock fragmentation ways.
The surface of AC8A Ah alloy was modified by adding the Cu powder using a Plasma Transferred Arc (PTA) welding process. Under the optimum fabricating conditions, the modified surface of AC8A Ah alloy was observed to possess the sound microstructure with a minimum porosity. Hardness and wear resistance properties of the as-fabricated alloy were compared with those of the 76 heat-treated one. In case of the as-fabricated alloy, the hardness of the modified layer was twice that of the matrix region. Although significant increase in the hardness of the matrix region was observed after T6 heat treatment, the hardness of the modified layer was not observed to change. The wear resistance of the modified layer was significantly increased compared to that of the matrix region. The microstructure of a weld zone and the matrix region were investigated using the optical microscope, scanning electron microscope (SEM), electron probe microanalysis (EPMA), and transmission electron microscope (TEM). The primary and eutectic silicon in the weld zone were finer and more curved than in the matrix region, while some precipitates has had been found therein. According to the TEM observation, the predominant precipitate present in the weld zone was the $\theta$'phase, which is precipitated during cooling by rapid solidification in PTA welding process. Improvement of hardness and wear properties in the weld zone in the as-fabricated condition can be explained based on the presence of $\theta$’precipitates and fine primary and eutectic silicon distribution.
To establish the optimum condition for plant regeneration from somatic embryos of Acanthopanax sessiliflorus Rupr. et Maxim, a medicinal plant, somatic embryos were induced from zygotic embryo-derived embryogenic callus in hormoen-free MS medium. To induce plantlet conversion, cotyledonary somatic embryos were cultured on MS solid medium with GA$_3$at various concentrations (0~10 mg/L) for three weeks. Plantlets were transferred to 1/3 MS solid medium with 0.5% charcoal for 7 weeks. Stem length was increased proportionally to the concentration and treatment period of GA$_3$. Also, the highest leaf width (8.9 mm) and leaf number (2.84) of plantlet were obtained when plantlets were converted on 5,10 mg/L GA$_3$pretreatments, respectively. The highest plant conversion frequency (66.7%) was obtained when the somatic embryos were cultured on medium containing 5 mg/L GA$_3$ for 3 weeks and then were transferred to 1/3 MS medium with 0.5% charcoal. The highest survival rate of soil transfer was 90% when plantlets were regenerated on medium with 5 mg/L GA$_3$ for 3 weeks and then transferred to plastic pots containing vermiculite and sand mixture for 4 weeks.
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