• Journal of Physiology & Pathology in Korean Medicine
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• v.35 no.2
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• pp.71-80
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• 2021

Parkinson's disease is one of the typical neurodegenerative disease and it is caused by the destruction of substantia nigra in brain leading to lack of dopamine secretion, and it presents 4 major motor symptoms such as tremor, bradykinesia, stiffness, postural instability. Furthermore, it causes many non-motor symptoms such as anosmia, REM sleep conduct disorder, orthostatic hypotension, dementia and autonomic ataxia such as lack of adjusting blood pressure, hyperhydrosis, constipation. Dopaminergic therapy is the most commonly used strategy, but long term treatment of levodopa induce various adverse effects. Thus, many people are focusing on new therapies other than established therapies, and there are many tries and approaches with paradigm shift. Our medical team was able to get 4 cases of PD patients who are hospitalized in our hospital, treated by Whole Body Gi-Hyeol Therapy consisting of acupuncture therapy, herbal therapy, and mental therapy, and their conditions improved in perspective of Unified Parkinson's Disease Rating Scale(UPDRS), Heart Rate Variability(HRV), and Quality of life. Among all 4 cases, UPDRS score and quality of life score is gotton better, and among 2 cases SDNN, RMS-SD, TP, LF, HF scores are finely increased. And PDQ-39 score which shows quality of life is also improved. However, in spite of these improvements and positive results, there were no meaningful improvement in a hurt from a fall which is important to the aged, muscular atrophy which causes bone fracture and SMI(Skeletal Muscle Mass Index) which is indicator of osteoporosis. Thus, supplementary treatment about Whole Body Gi-Hyeol Therapy such as more active nutrition intervention, safe and effective kinesitherapy is needed, and from now on continuous case reports and systematic clinical research which has control group must be carried out.

• Journal of agriculture & life science
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• v.52 no.6
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• pp.89-101
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• 2018

The purpose of this study was to evaluate the variation of water quality in the Nakdong River by the Four major river projects. For this purpose, the nearest upstream water quality monitoring stations were selected and water quality data such as BOD, COD, SS, TN, TP, and TOC were collected for the before Four major river project(from January 2003 to November 2008), during the project(from September 2008 to April 2012) and after the project(from May 2012 to September 2017). As a result, the concentration of DO, BOD, COD, TN, and TOC after the project tend to be higher than the before project period. On the other hand, the SS and T-P values decreased after the project. Spatial analysis of BOD, COD, and TOC, which have large changes in water quality factor, by water quality grade, showed a tendency that water quality grade after the project was worse than before the project.

• Journal of Wetlands Research
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• v.20 no.4
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• pp.314-321
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• 2018

In order to quantify nonpoint source pollution, it was proposed to sample at regular intervals of 1 hour for the first 24 hours of storm runoff process by National Institute of Environmental Research for the mixed landuse watershed. However, high frequency sampling requires intensive laboratory analysis and labor costs. In order to investigate the effect of longer sampling interval on the load estimation compared to the 1 hour sampling method, analysis was conducted using monitoring data from rural subwatershed, urban subwatershed, and outlet of the Pungyeongjeongcheon watershed. Statistical analysis revealed that mean of load estimation was not significantly different up to 4 hour sampling frequency. However, 3 hour sampling interval was found to be appropriate for the BOD and TP when it is judged that 10% or less of the difference in loading amount between the 1 hour and other sampling interval is reasonable. The results of this study can be used to conduct an effective monitoring system.

• Journal of Life Science
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• v.29 no.1
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• pp.52-59
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• 2019

The leave of Polygonum tinctorium (LPT) have been used for centuries as a traditional medicine and as a food ingredient and natural dye. The aim of the current study was to develop high-value added products using LPT. Hot water extract (HWE) and ethanol extract (EE) of LPT were prepared, respectively, and their bioactivity was evaluated. The extraction ratio for the HWE was 27.6%, which was two-fold higher than that of the EE. The contents of total polyphenol in the HWE and total sugar in the EE were 51.2 mg/g and 297.8 mg/g, respectively. The total flavonoid and reducing sugar contents were similar in the extracts, irrespective of the extraction solvent. The HWE did not show antimicrobial activity in a disc-diffusion assay, but the EE showed strong growth inhibition against gram-positive bacteria. The EE exhibited stronger DPPH and ABTS radical scavenging activities and reducing power than those of the HWE. The HWE was particularly effective as a scavenger of nitrite ($RC_{50}$ of $6.0{\mu}g/ml$). In an antithrombosis activity assay, the EE showed significant anticoagulation activity as determined by an extended blood coagulation time (thrombin time, prothrombin time, and activated partial thromboplastin time), in addition to platelet aggregation activity. The HWE also showed platelet aggregation inhibitory activity. This report provides the first evidence of antithrombosis activities of LPT. Our results suggest that LPT has potential as a new antioxidant and antithrombosis agent.

• Journal of Environmental Science International
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• v.28 no.3
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• pp.303-320
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• 2019

Water quality is characterized by various complex factors. Therefore, a systematic understanding of water quality trends is required to carry out a proper evaluation. In this study, we analyzed the spatio-temporal water quality characteristics of the Nakdong River using five-year data from 2012 to 2016. Data was collected on the pH, DO, BOD, COD, SS, TN, TP, TOC, WT, EC, $NH_3-N$, $NO_3-N$, $PO_4-P$, Chl-a, rainfall, and total and fecal coliforms. A total of 38 water quality measurement stations, from Andong1 to Gupo, were considered. Statistical analyses including trend, cluster, and factor analyses were conducted to identify the dominant water quality components affecting the Nakdong River. The Nakdong River was spatially classified into three groups for up-stream (Andong1 to Sangju1), mid/up-stream (Donam to Dalseong), and mid/down-stream (Hwawonnaru to Gupo) data collection, and temporally into two groups for summer/fall (7~10), and the rest of the season (11~6) data. The water quality of the entire Nakdong River showed trends similar to the mid/down-stream section, which indicates the importance of water quality management in this section. Suspended solids, phosphorus, and coliform groups were established as important factors to be considered in the summer/fall season across the river, especially in the mid/down-stream section. Nitrogen and organic matter were identified as important factors to be considered in the rest of the season, especially in the mid/up-stream section. This study could help determine the water quality components that should be intensively monitored in the Nakdong River.

• Korean Journal of Environmental Biology
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• v.38 no.4
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• pp.678-690
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• 2020

In this study, the histological changes of Zacco platypus exposed to discharge from a Wastewater Treatment Plant (WTP), which is a point source, for a long time (2 to 3 years) were compared to the same species at a reference site (Ref.). Overall, tissues displayed various lesions in samples obtained at the point where discharge water from the point source was mixed. In the skin tissue, mucus cells from the epithelial layer expanded to the multilayered epithelium, indicating the immune system was activated. Epithelial cell detachment and proliferation were most prominent in the gills, which may have adversely affected circulation and respiration. Our data suggest immune system collapse was due to stimulation by aquatic substances. Both the fish phase analysis and the water quality analysis demonstrated depreciated conditions at the point source as compared to the reference stream, supporting the histological health evaluation results. These data together suggest a histological approach can also be used to assess water quality, and to an even higher degree when combined with other existing methods. Given the presented evaluation, improvement in the water quality of water discharged from WTP's is required.

• Korean Journal of Ecology and Environment
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• v.55 no.4
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• pp.320-329
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• 2022

Urban areas in watersheds increase the impervious surface, and agricultural areas deteriorate the water quality of rivers due to the use of fertilizers. As such, anthropogenic land use affects the type, intensity and quantity of land use and is closely related to the amount of substances and nutrients discharged to nearby streams. Riparian vegetation reduce the concentration of pollutants entering the watershed and mitigate the negative impacts of land use on rivers. This study analyzes the data through correlation analysis and regression analysis through point data measured twice a year in spring and autumn in 21 selected damaged tributary rivers within the Han River area, and then uses a structural equation model to determine the area land use. In the negative impact on water quality, the mitigation effect of riparian vegetation was estimated. As a result of the correlation analysis, the correlation between the agricultural area and water quality was stronger than that of the urban area, and the area ratio of riparian vegetation showed a negative correlation with water quality. As a result of the regression analysis, it was found that agricultural areas had a negative effect on water quality in all models, but the results were not statistically significant in the case of urban areas. As a result of the model estimated through the structural equation, BOD, COD, TN, and TP showed a mitigation effect due to the accumulation effect of river water quality through riparian vegetation in agricultural areas, but the effect of riparian vegetation through riparian vegetation was found in urban areas. There was no These results were interpreted as having a fairly low distribution rate in urban areas, and in the case of the study area, there was no impact due to riparian forests due to the form of scattered and distributed settlements rather than high-density urbanized areas. The results of this study were judged to be unreasonable to generalize by analyzing the rivers where most of the agricultural areas are distributed, and a follow-up to establish a structural equation model by expanding the watershed variables in urban areas and encompassing the variables of various factors affecting water quality research is required.

• Korean Journal of Clinical Laboratory Science
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• v.55 no.1
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• pp.16-28
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• 2023

Lung adenocarcinoma accounts for about 40% of all lung cancers. With the recent development of gene profiling technology, studies on mutations in oncogenes and tumor suppressor genes, which are important for the development and growth of tumors, have been actively conducted. Companion diagnosis using next-generation sequencing helps improve survival with targeted therapy. In this study, formalin-fixed paraffin-embedded tissues of non-small cell lung cancer patients were subjected to hematoxylin and eosin staining for detecting genetic mutations that induce lung adenocarcinoma in Koreans. Immunohistochemical staining was also performed to accurately classify lung adenocarcinoma tissues. Based on the results, next-generation sequencing was applied to analyze the types and patterns of genetic mutations, and the association with smoking was established as the most representative cause of lung cancer. Results of next-generation sequencing analysis confirmed the single nucleotide variations, copy number variations, and gene rearrangements. In order to validate the reliability of next-generation sequencing, we additionally performed the existing genetic testing methods (polymerase chain reaction-epidermal growth factor receptor, immunohistochemistry-anaplastic lymphoma kinase (D5F3), and fluorescence in situ hybridiation-receptor tyrosine kinase 1 tests) to confirm the concordance rates with the next-generation sequencing test results. This study demonstrates that next-generation sequencing of lung adenocarcinoma patients simultaneously identifies mutation.

• Biomolecules & Therapeutics
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• v.31 no.1
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• pp.73-81
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• 2023

Sirtuins (SIRTs) belong to the nicotinamide adenine dinucleotide (NAD+)-dependent class III histone deacetylase family. They are key regulators of cellular and physiological processes, such as cell survival, senescence, differentiation, DNA damage and stress response, cellular metabolism, and aging. SIRTs also influence carcinogenesis, making them potential targets for anticancer therapeutic strategies. In this study, we investigated the anticancer properties and underlying molecular mechanisms of a novel SIRT1 inhibitor, MHY2251, in human colorectal cancer (CRC) cells. MHY2251 reduced the viability of various human CRC cell lines, especially those with wild-type TP53. MHY2251 inhibited SIRT1 activity and SIRT1/2 protein expression, while promoting p53 acetylation, which is a target of SIRT1 in HCT116 cells. MHY2251 treatment triggered apoptosis in HCT116 cells. It increased the percentage of late apoptotic cells and the sub-G1 fraction (as detected by flow cytometric analysis) and induced DNA fragmentation. In addition, MHY2251 upregulated the expression of FasL and Fas, altered the ratio of Bax/Bcl-2, downregulated the levels of pro-caspase-8, -9, and -3 proteins, and induced subsequent poly(ADP-ribose) polymerase cleavage. The induction of apoptosis by MHY2251 was related to the activation of the caspase cascade, which was significantly attenuated by pre-treatment with Z-VAD-FMK, a pan-caspase inhibitor. Furthermore, MHY2251 stimulated the phosphorylation of c-Jun N-terminal kinase (JNK), and MHY2251-triggered apoptosis was blocked by pre-treatment with SP600125, a JNK inhibitor. This finding indicated the specific involvement of JNK in MHY2251-induced apoptosis. MHY2251 shows considerable potential as a therapeutic agent for targeting human CRC via the inhibition of SIRT1 and activation of JNK/p53 pathway.

• Korea Science and Art Forum
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• v.19
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• pp.515-525
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• 2015

Along with a rapid increase in the number of 1-person households where the concept of 1-person consumption and 1-person economy is emphasized, the proportion of 1-person household in Korea occupies a 25.3% at present, and the number is expected to grow continuously. And this rapid increase will bring about a host of problems such as housing shortage in the metropolitan area, egoism, loneliness felt by individuals, and other social problems such as crime. As an alternate movement to this phenomenon, concern on and interest in community, community culture, and sharing of space are on the rise, together with concern on and preference for sharehouse culture. In news media as well, articles on life shared with members of a sharehouse often appear. This sharehouse, which is widely spread and well received in Japan, not only reduces economic burden but also creates their own community and promotes their own culture. In this sense, it is a new way of life that represents benefits of sharing, well beyond just economic interests. Accordingly, In this research, an attempt was made tp examine the circumstances in the use of space based on existing studies on sharehouse characteristics in order to shed new lights on the meaning that non-characteristic space concept of the traditional residential space has as the concept of sharehouse space by considering it in connection with the non-characteristic space concept of the Korean traditional residential space where diverse circumstances occur centered on communal life and which were accepted by all naturally.