• Mycobiology
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• v.50 no.5
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• pp.366-373
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• 2022

Regulation of proper gene expression is important for cellular and organismal survival, maintenance, and growth. Abnormal gene expression, even for a single critical gene, can thwart cellular integrity and normal physiology to cause diseases, aging, and death. Therefore, gene expression profiling serves as a powerful tool to understand the pathology of diseases and to cure them. In this study, the difference in gene expression in Flammulina velutipes was compared between the wild type (WT) mushroom and the mutant one with clogging phenomenon. Differentially expressed transcripts were screened to identify the candidate genes responsible for the mutant phenotype using the DNA microarray analysis. A total of 88 genes including 60 upregulated and 28 downregulated genes were validated using the real-time quantitative PCR analysis. In addition, proteomic differences between the WT and mutant mushroom were analyzed using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF). Interestingly, the genes identified by these genomic and proteomic analyses were involved in stress response, translation, and energy/sugar metabolism, including HSP70, elongation factor 2, and pyruvate kinase. Together, our data suggest that the aberrant expression of these genes attributes to the mutant clogging phenotype. We propose that these genes can be targeted to foster normal growth in F. velutipes.

• The Journal of Bigdata
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• v.6 no.1
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• pp.145-151
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• 2021

In recent years, digital data has been generated in all areas of human activity, and there are many attempts to safely store and process the data to develop useful services. A data lake refers to a data repository that is independent of the source of the data and the analytical framework that leverages the data. In this paper, we designed a tool to safely store various big data generated by smart cities in a data lake and ETL it so that it can be used in services, and a web-based tool necessary to use it effectively. Implement. A series of processes (ETLs) that quality-check and refine source data, store it safely in a data lake, and manage it according to data life cycle policies are often significant for costly infrastructure and development and maintenance. It is a labor-intensive technology. The mounting technology makes it possible to set and execute ETL work monitoring and data life cycle management visually and efficiently without specialized knowledge in the IT field. Separately, a data quality checklist guide is needed to store and use reliable data in the data lake. In addition, it is necessary to set and reserve data migration and deletion cycles using the data life cycle management tool to reduce data management costs.

• The Journal of Korean Association of Computer Education
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• v.20 no.6
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• pp.17-25
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• 2017

In order to ensure that the 2015 revised curriculum is successfully settled at the field of school, there are 68 national SW schools operated by 2015 and 2016 at national level, and an inspection tool was developed to measure the effectiveness of SW education. The purpose of this study is to present the direction of the computing thinking inspection tool which can verify the effectiveness of the curriculum from 2018 through the analysis of the previously developed computing thinking inspection tool. As a result of the analysis, the validity of the ability factors to measure the problem solving process based on computing thinking was not enough. In the analysis of the item characteristics, the degree of discrimination was good, but difficulty was not enough to accurately measure the competence of students because of the high percentage of easy questions. Based on the results, this study suggested the development of the items considering the problem solving process based on the computing thinking, the application of the students' empirical knowledge, and the constitution that can confirm the flow of the thinking power with materials in the real life.

• Journal of Korean Academy of Nursing
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• v.26 no.4
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• pp.743-758
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• 1996

This paper was done to verify the use of the Profile of Mood States (POMS) which was developed by McNair, Lorr, and Droppleman(1992) with modifications so that it is appropriate for Korean elders. Through the modified tool, it is possible to examine the mood of Korean elders and to contribute to the welfare of elders. The subjects were 370 elderly persons over 60 years old and the data for 319 persons(86%) were analyzed. The research tool was the POMS translated by Yun(1993) and corrected by the researcher. Data were collected between February 12 and April 9 in 1996 and analyzed using the SAS package. The result are as follows 1. Items with low Cronbach coefficient alpha which means low correlation with total items were removed. The items were removed : friendly, tense, considerate, relaxed, sympathetic, resentful, good-natured, rebellious, trusting, carefree. 2. Overlapped or ambiguous items were discussed by colleagues and elders through verification of con-tent-validity and were removed. Five items were removed in tense-anxiety, seven, in depression-dejection, three, in vigor-activity, three, in fatigue-inertia, two, in confusion-bewilder-ment, five, in friendliness, and six, in anger-hostility. Thirty four items remainined : angry, clear-headed, lively, confused, sorry, shaky, list-less, peeved, sad, active, blue, hopeless, spiteful, uneasy, unable to concentrate, fatigued, helpful, nervous, lonely, cheerful, bitter, exhausted, anxious, ready to fight, sluggish, helpless, alert, deceived, efficient, worthless, forgetful, terrified, vigorous, and uncertain about things. 3. Factor analysis was done in order to confirm construct validity and three factors were obtained from the result. The first factor, 'anxiety-depression' included 21 items, the second factor, 'vigor' included eight items, and the third factor, 'anger' included five items. Cronbach coefficient alpha for the 34 items was. 95. Based on the result, the following is suggested : 1. a contribute to elder's welfare can be made by examining Korean elder's mood in life. 2. there is a need to develop tools appropriate to Korean culture which can be used to examine elders' mood. 3. The modified POMS tool needs to be reverified with appropriate age groups and settings.

• Journal of the Korean Society of Systems Engineering
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• v.16 no.1
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• pp.18-24
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• 2020

The KSLV-II project with high difficulties technically requires thorough technical management during long-term life cycle more than 10 years for launching into space. The TRL is a quantitative indicator developed by NASA widely used all over the world to measure technology maturity of a system development objectively and consistently. The TRL is also used to make sure technology level and to establish a future direction in the KSLV-II project. The TRL has advantage enable to identify a technology level through quantitative indicators. However, it takes a lot of efforts such as trials and errors, time and cost to apply it to the project considering the project environments, and stakeholder needs. These include not only to establish TRL management plan from ideal, conceptual and abstractive standards/guidelines such as NASA's, but also to construct TRL management environment enable to apply and manage harmoniously. In the KSLV-II project, it is required to figure out current technology level and technology development trend in the future, to access conveniently, to share related data in real time, and to update periodically for the comprehensive TRL management. From the reason above, the TRL management environment was built by using the systems engineering tool already has been used for other system management data such as requirements in the project. It also could be accomplished a practical management basis of systems engineering from the traceability among system management data including TRL. In this paper, case study results are introduced to manage the TRL for the space launch vehicle using the systems engineering tool in the KSLV-II project.

• KIPS Transactions on Computer and Communication Systems
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• v.5 no.11
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• pp.427-438
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• 2016

Car blackboxes record the information and status of driving. Since blackboxes are commonly used in daily life, the usage of video data recorded from blackboxes is increasing for investigating. Investigators use a own analysis tool suitable for their blackbox provided by the manufacturer in order to check the data. But the tools are not enough to use in the digital forensic analysis because they are dependent on a specific model of blackbox and provides ungeneralized functions. Moreover, if the manufacturer is bankrupt, then their own tools can not be obtained also. Therefore, the way data are stored in the blackboxes which are now in the market are investigated and the features and limitations which have blackbox's own analysis tools are checked. And a comprehensive tool for the analysis of blackboxes is designed and implemented as in this paper.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2013.10a
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• pp.275-278
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• 2013

Almost teachers have some basic questions about the effective teaching and learning. The purpose of this study is to develop an alternative tool to observe and analyze an instruction based on the learning theories providing the theoretical rationalities for learners to study not only in daily their life but also in their class and to apply it as an example. This study showed some suggestions analyzing an instruction theoretically through out some filters based on learning theories. Recently various learning theories have been studied and accumulated. This study developed an alternative tool to observe and analyze an instruction based on the Behaviorism, the Cognitivism and the Constructivism in the middle of these learning theories and it was applied to observe and analyze a actual class of the informatics subject. The expectation is for teachers to reflect or improve their classes using the alternative tool.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.5 no.4
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• pp.198-205
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• 2012

This study deals with development of an evaluating tool for flow experience and presence to evaluate interactive digital contents. The tool is able to measure the grade of flow and presence by surveying with their factors which are known to affect flow experience and presence. One of reasons for reducing flow experience and presence in 3D digital contents is that the experience in the virtual world is different from user's prerequisite learning in the real life. The recent interactive contents using physical movement of users as an input is possible to provide unsafe situation to users due to the different experience. The suggested flow measurement tool is able to evaluate presence and flow experience of an interactive 3D contents as well as flow and presence factors are possible to use as a general guideline for all stages of producing interactive 3D digital contents.

• Korean Journal of Agricultural Science
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• v.38 no.4
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• pp.775-783
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• 2011

The purpose of this study was to investigate if an community forestry programme (HKm) could be one of the main tool for REDD+ in Indonesia. Recently, considerable numbers of countries including Norway, England, Korea and etc. have invested 69 million US$ in total to Indonesia for implementation of REDD+. However, forest dependent people without a communal right are subject to poor forest governance during a stream of REDD+ in Indonesia. This study suggest that HKm are needed to be a tool for REDD+ in order to achieve the objects of REDD+ in terms of Equity, Efficiency, and Effectiveness (3Es) by stimulating local and Indigenous peoples to participate in REDD+. As a result of our study, Indonesia government have designated only 200,000 ha for HKm area by 2007. Indonesia government have a week political will to extend its area to give a communal right to those peoples who dwell in state forests and HKm application process is complicated and slow. HKm has a potential to be main tool for REDD+ in Indonesia. However HKm will be difficult to be main tool for REDD+ without resolving current obstacles.

• Journal of the Korea institute for structural maintenance and inspection
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• v.20 no.4
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• pp.77-83
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• 2016

Recently, interests for maintenance of transmission tower are increasing to extend life of structures and reduce maintenance cost. However, existing classical diagnosis method of corrosion deteriorated degree on the transmission tower steel members, visual inspection, has a problem that error often due to difference of inspector's individual knowledge and experience. In order to solve the problem, this study carried out to develop the corrosion deterioration inspection tool for transmission tower steel members. This tool is composed of camera equipment and computer-aided diagnosis system. We standardized the photographing method by camera equipment to obtain suitable pictures for image processing. Diagnosis system was designed to evaluate automatically degree of corrosion deterioration for member of transmission tower on the basis of the RGB color image processing techniques. It is anticipated that developed the corrosion deterioration inspection tool will be very helpful in decision of optimal maintenance time for transmission tower corrosion.