Mycobiology

The Korean Society of Mycology (한국균학회)
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Gene Expression Analyses of Mutant Flammulina velutipes (Enokitake Mushroom) with Clogging Phenomenon

  • Ju-Ri Woo (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Doo-Ho Choi (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Muhammed Taofiq Hamza (Department of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kyung-Oh Doh (Department of Physiology, College of Medicine, Yeungnam University) ;
  • Chang-Yoon Lee (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Yeon-Sik Choo (Department of Biology, College of National Sciences, Kyungpook National University) ;
  • Sangman Lee (Department of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Jong-Guk Kim (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Heeyoun Bunch (Department of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Young-Bae Seu (School of Life Science and Biotechnology, Kyungpook National University)
  • Received : 2022.07.05
  • Accepted : 2022.09.01
  • Published : 2022.10.31
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Abstract

Regulation of proper gene expression is important for cellular and organismal survival, maintenance, and growth. Abnormal gene expression, even for a single critical gene, can thwart cellular integrity and normal physiology to cause diseases, aging, and death. Therefore, gene expression profiling serves as a powerful tool to understand the pathology of diseases and to cure them. In this study, the difference in gene expression in Flammulina velutipes was compared between the wild type (WT) mushroom and the mutant one with clogging phenomenon. Differentially expressed transcripts were screened to identify the candidate genes responsible for the mutant phenotype using the DNA microarray analysis. A total of 88 genes including 60 upregulated and 28 downregulated genes were validated using the real-time quantitative PCR analysis. In addition, proteomic differences between the WT and mutant mushroom were analyzed using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF). Interestingly, the genes identified by these genomic and proteomic analyses were involved in stress response, translation, and energy/sugar metabolism, including HSP70, elongation factor 2, and pyruvate kinase. Together, our data suggest that the aberrant expression of these genes attributes to the mutant clogging phenotype. We propose that these genes can be targeted to foster normal growth in F. velutipes.

Keywords

Acknowledgement

This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education [2016R1A6A1A05011910] and Research Institute for Dokdo and Ulleung-do Island of Kyungpook National University, Korea.

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