• 한국연초학회지
• /
• 제21권2호
• /
• pp.152-159
• /
• 1999

A CAB cDNA clone(pKGCAB) encoding the light harvesting chlorophyll a/b binding protein of the semi-shade plant, Korean ginseng(Panax ginseng C. A. Meyer) was isolated by the one-way path random sequencing of ginseng cDNA library clones and transgenic tobacco plants(Nicotiana tabacum NC82) were produced by the transformation of this ginseng CAB gene in use of Agrobacterium tumefaciens LBA4404. The CAB gene showed type 1 structure of LHCP-II, 84% similarity in nucleotide sequence and 92% in amino acid sequence to that of Nicotiana tabacum CAB40, respectively. Seed germination and initial growth of the transgenic tobacco plants transformed with the cDNA fragment were accelerated under low light intensity compared with those of normal tobacco plant, that may result from the higher light sensitivity of the transgenic plants than that of the normal.

• 한국연초학회지
• /
• 제11권2호
• /
• pp.143-148
• /
• 1989

Field experiment was conducted to find out the chemical characteristics of 12 Korean local varities, Local varities were compared with oriental, flue-cured and burley tobacco. Among tested varities, Hyangcho and Matskawa were desirable in respect of flavor, leaf color and texture. Hyangcho and Matskawa were higher in the content of nicotine and petroleum ether extract. Gajacho, Byeolcho and Kukjuo were higher in the content of reducing sugar, but lower nicotine, total nitrogen and petroleum ether extract. The nicotine and total nitrogen content of Kwangcho, Daruma and Catterton were lower than that of Br. 21. In the content of volatile organic acid which were major characteristic component of oriental tobacco, the Korean local varities were lower in the content of 3-methyl butanoic and 3-methyl pentanoic acid, but hirities, Hyangcho, Matskawa and Daruma were higher in the content of volatile organic acid. Korean local varities, especially small leaf group, were higher in the content of benzyl alcohol and furfural than any other type tobacco.

• 한국연초학회지
• /
• 제23권1호
• /
• pp.90-93
• /
• 2001

In order to save hanging labor hours and reduce work intensity for bulk curing of the flue-cured tobacco, and electric hoist was developed. It consists of a pair of square steel frames with side plates and rolling casters to move back and forth on the second tire of bulk barn, and wire rope type lift was attached to the beneath of its top center. The lift driven by DC motor could be controlled by operating switch with one limit switch and control box, and a scissors shaped gripper was deviced to grip tobacco rack to the end of wire rope. As the results of experiments with electric hoist, labor hours for hanging could be saved by 30 to 40% as compared with those in conventional method. With simple and light structure, it was possible for even woman to load the harvested tobacco, and it could be recommended for farmers to use this device with reasonable price.

• 한국작물학회지
• /
• 제5권1호
• /
• pp.65-68
• /
• 1969

잎담배의 다수성품종 육성의 선택에 앞서 각형질상호간의 상관관계를 구명하고 이들 형질이 수량에 어떻게 관여하는가를 분석함으로써 다수성 계통을 용이하게 선택할수 있겠으므로 이들 주요형질을 고찰한바 잎담배 수량에 직접상으로 관여하는 것은 황색종에서는 엽폭, 엽면적이었고 양건종에서는 간주, 엽수, 엽장, 엽면적이며 음건종에서는 간주, 엽수, 엽장, 엽폭, 엽면적이었다. 종류를 막론하고 엽폭과 엽면적이 수량에 크게 작용하며 이중 가장 측정이 용이하고 실제 다수성개체선택에 효과적인 형질은 엽폭이라 할 수 있다.

• 한국연초학회지
• /
• 제21권1호
• /
• pp.77-81
• /
• 1999

Tobacco plants(Nicotiana tabacum cv. NC82) transformed with TMV CP cDNA were self-fertilized until 8th generation (R$_{8}$), and the transgenic plants from 6th to 8th generation were analized for their resistance to tobacco mosaic virus(TMV) and stability of the gene expression. The 6th generation of the plants(R$_{6}$) showed high resistance(81-91 %) to TMV at eight weeks after artificial inoculation with the virus. The transgenic cell line 601 was the most prominant in the expression of resistance. 98 % of the plants showed no symptom without any agronomic phynotepe variation when they were inoculated with the virus in a experimental field. However, 2% of the plants were revealed as delay type of symptom with mild mosaic on a few leaves. The viral resistance in greenhouse tests of the 7th generation (R$_{7}$) was 54-64%, and the number of delay type plants were increased than that of 6th generation plants. In the 8th generation, 81 % of the plants was complete resistant to the virus. The TMV CP cDNA of the transgenic plants of each generation was also confirmed by genomic PCR, and there was no systemic viral multiplication in the resistant plants. It suggests that the viral resistance and gene expression of the transgenic plants might be stable through the generations.ons.s.

• 펄프종이기술
• /
• 제40권2호
• /
• pp.65-72
• /
• 2008

This work evaluated the extractability of tobacco stem biomass for the papermaking type Reconstituted Tobacco Sheet(RTS). The effects of the soaking conditions on the hydration of stem biomass and the effects of the hydrated state on the mechanical extraction were investigated. In order to simulate the mechanical expression process of a papermaking type RTS mill, for example, the screw press process, the novel mechanical pressing analyzer was developed for this study. The hydration of stem biomass by soaking process was greatly affected by the soaking time and the soaking temperature. The longer soaking time and the higher soaking temperature resulted in the higher hydrated stem biomass. Since the higher hydrated stem had more combined water in the inner structure and resulted in the more flexible structure, the higher hydrated stem leaded to the more compressed filter cake and the higher water contents in the filter cake after the mechanical pressing. The pilot pulping experiments showed the difference in hydration and extractability between burley and bright tobacco stem. The bulkier structure of the burley stem resulted in the faster hydration by pilot pulping and leaded to the larger reduction in water soluble components. And the hydration process showed the major influence on the separation efficiency of water soluble components.

• 한국연초학회지
• /
• 제31권2호
• /
• pp.85-94
• /
• 2009

This work has been conducted to develop a method for the analysis of neutral volatile flavors and their precursors in tobacco. The neutral volatile compounds and precursors in tobaccos have been investigated by Neutral Volatile scan method(NV scan) using Soxhlet extractor. The method has been used to analyze a range of different tobaccos and tobacco products. Neutral flavor compounds were classified as three sections(1st Volatile Fraction, Breakdown Flavor Products and Cembranoid Precursors). The major components of the First Volatile Fraction were 2-cyclohexene-1-one, 6-methyl-5-hepten-2-one, limonene and phenyl ethanol. The major components of Breakdown Flavor Products were isophorone, solanone, damascenone, 3-hydroxy-$\beta$-damascone, geranyl acetone, $\beta$-ionone, dihydroactinidiolide, norsolanadione, neophytadiene, hexahydrofarnesylacetone, farnesyl acetone and megastigmatrienone. The major cembranoid precursor compounds were dibutyl phthalate, duvatrenediols, 8,12-epoxy-14-labden-13-ol, 11-hydroperoxy-2,7,12(20)-cembratriene-4,6-diol, 12,15-epoxy-12,14-labadien-8-ol, 2,7,11-cembratrien-4,6-diol and 8,13-epoxy-14-labdien-12-ol. The NV scna results of tobacco types(flue-cured, burley and oriental) showed that each tobacco type has a characteristic flavor component profile.

• 한국연초학회지
• /
• 제22권1호
• /
• pp.71-75
• /
• 2000

The genetic makeup could be the most important among many factors affecting yield and quality of tobacco(Nicotiana tabacum L.). The mammoth gene found in N. tabacum is associated with greater leaf number and poor leaf quality. This study was carried out to obtain the basic information about the inheritance of mammoth gene and white flower color. Two flue-cured breeding lines, KF 9373-2 and KF 8832-85, F$_1$, F$_2$, two parents backcrossed with F$_1$, and F$_3$ lines derived from cross of above two lines were investigated for flowering type(mammoth gene) and flower color. All plants of F$_1$ population revealed normal flowering type and pink flower color. The progeny of F$_2$ generation was segregated into the phenotypic ratio of 9 : 3 : 3 : 1 with normal flowering type and pink flower color, normal and white, non flowering type(NF) and pink, and NF and white, respectively. Among the progenies of back-crossing populations, the flowering type showed a segregation ratio of 1 : 1 as normal and NF in BP$_1$ and flower color did also 1 : 1 as pink and white in BP$_2$. All lines have the mammoth gene in F$_3$. that were selected in F$_2$ progeny as non flowering. But 9 lines among 14 were segregated with 3 : 1 as pink and white flower in F$_3$. which were selected in F$_2$ as pink flower color. These results indicated that the characters of mammoth gene and white flower were controlled by a pair of recessive genes, respectively.

• Journal of Ginseng Research
• /
• 제13권2호
• /
• pp.239-241
• /
• 1989

Naloxone partially antagonized the analgesic effect of a large dose of morphine and inhibited the development of an acute type tolerance. Ginseng total saponins did not antagonize the analgesia of a large dose of morphine but inhibited the delrelopment of acute and delayed types tolerance. The morphine analgesia and the development of acute type tolerance were affected by the opioid receptor antagonist, naloxone, but the development of acute type tolerance was not. Ginseng total saponins partially inhibited the development of the delayed type tolerance that was not inhibited by naloxone, but also partially suppressed the development of the acute type tolerance that was completely inhibited by naloxone. These results imply that the partial inhibition of the development of the acute and delayed types tolerance by ginseng total saponins is not mediated by the opioid receptors.

• 한국연초학회지
• /
• 제26권2호
• /
• pp.117-125
• /
• 2004

In this study, we have investigated the natural and puffing combustibility of cigarette brands and the compulsory ash-drop, and analysed factors related to the materials. Cigarette combustibility was greatly affected by the tobacco weight in cigarettes rod. Combustion rate of 4AOR cigarette made by flue-cured tobacco was 5.02 $^{mm}/_{min}\;and\;10\%$ faster than 1BO cigarette. Combustion pattern has interrelation with sugars, chlorine and ash contents among tobacco components. Combustion rate of 4DL, flue-cured tobacco was 55 seconds faster than 1CL tobacco extremely much in sugars contents, and ash drop was maintained at $21\%$ longer. Combustion time and burning rate were fast in cigarette made by expanded tobacco slice and tobacco stem and the formation of ash was poor. The length of puffed ash drop of expanded tobacco slice and tobacco stem were 18.6 mm and 25.2 mm and these forced ash drop length were 3.8 mm and 4.9 mm respectively. Puffed burning rate of general cigarette was $14.3\%$ faster than static burning rate and super slim type cigarettes was $21.3\%$ faster. Combustion rate and ash drop of domestic and foreign cigarette brands were quite different depending on tar level and cigarette types. The lower tar level, the faster combustion rate and the shorter ash drop.