• Asian-Australasian Journal of Animal Sciences
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• 제22권1호
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• pp.42-48
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• 2009

An in vitro incubation study was conducted to investigate effects of 2-bromoethanesulfonic acid (BES) on ruminal fermentation characteristics and methanogen population. BES at the final concentration of 0, 1 and 5 mM with two different substrates having a different ratio of timothy and concentrate (100% timothy vs. 40% timothy-60% concentrate) was incubated for 0, 24, 48 and 72 h in a $39^{\circ}C$ incubator. Total DNA extracted from culture fluid was used as a template for real-time PCR to measure the population of methanogens. Four different primer sets were used for amplification of total bacteria, total methanogens, the order Methanobacteriales and the order Methanomicrobiales. BES reduced (p<0.01) total gas and methane production in a dose-dependent manner. BES at 5 mM inhibited methane production by more than 95% compared to the control. An interaction between substrate and level of BES in total gas and methane was detected (p<0.01). The decrease of methane production with increasing BES level was more pronounced on mixed substrate than on timothy alone. However, hydrogen production was increased by BES treatment (p<0.01). Total VFA concentration was not affected, but molar percentage of propionate and butyrate was increased and acetate to propionate ratio was reduced by BES treatment (p<0.01). BES did not affect the population density of total bacteria but reduced (p<0.01) the population of total methanogens, the order Methanobacteriales and the order Methanomicrobiales in a dose-dependent manner. The type of substrate did not influence the trend, although the magnitude of response was different between all-roughage and 40% roughage substrate.

• 농업과학연구
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• 제47권4호
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• pp.941-949
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• 2020

This study evaluated the nutritional value of Italian ryegrass (IRG) as a forage source for Hanwoo. The nutritional value of IRG was assessed and compared to that of rice straw, oat hay, and timothy hay using two different methods: 1) in vitro ruminal fermentation 2) in vivo total tract digestibility. In vitro DM digestibility was lower in rice straw compared to the other three forage sources after both 24 and 48 h of incubation (p < 0.01). Among the four forage sources, IRG had a higher NH3-N concentration after both 24 and 48 h of incubation (p < 0.01). In the in vivo digestibility trials, four different substrates were used: 1) 80% concentrate with 20% rice straw, 2) 80% concentrate with 20% oat hay, 3) 80% concentrate with 20% IRG, and 4) 80% concentrate with 20% timothy hay. The dry matter, crude protein, non-fiber carbohydrate, and detergent fiber digestibility were the greatest in the C80-IRG20 among the four forage groups. In summary, IRG had a similar level of energy efficiency compared to oat hay and timothy hay. Furthermore, the result of the chemical composition analysis showing a higher ammonia concentration in the in vitro fermentation experiment and the high protein digestibility in the in vivo experiment indicate that IRG is a good source of protein compared to oat hay and timothy hay.

• 한국초지조사료학회지
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• 제23권3호
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• pp.211-218
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• 2003

본 시험은 티모시 8품종 Climax(대조), Itasca, Alma, Comtal, Liphlea, Erecta, Argus 및 Kunpu를 축산기술연구소 대관령지소와 남원지소에서, 오차드그라스 7품종 Potomac(대조), Warrior, Ambassador, Hapsung 2호, Hapsung 20호, 93E 및 Kitamidori를 대관령지소에서 1999년부터 2002년까지 수행하였다. 티모시의 풍엽성은 Liphlea, Erecta 및 Kunpu가 다른 품종들에 비하여 좋았으며 출수시기도 Liphlea와 Kunpu가 다른 품종에 비하여 매우 빨랐다. 초장은 대조품종 Climax보다 길은 품종은 Kunpu로 다른 품종에 비하여 월등히 길었으며 월동성은 Erecta와 Kunpu가 94.0%로 가장 높았다. 오차드그라스의 풍엽성은 Hapsung 20호가 가장 우수하였고, 오차드그라스의 출수시기는 대체로 늦은 편에 속하였으나 그 중에서 Potomac이 가장 빨랐다. 또한 초장은 Hapsung 2호가 가장 길었으며 월동성도 가장 우수하였다. 티모시의 건물수량은 대관령과 남원에서 Kunpu가 각각 9,493와 13,890kg/ha로 가장 많았다. 대관령지역에서 오차드그라스의 건물수량은 국내 육성품종인 Hapsung 2호와 Hapsung 20호가 각각 8,690과 8,818kg/ha으로 가장 많았다. 두 지역에서 티모시의 ADF 평균 함량은 Climax가 33.4%로 가장 낮았고 Kunpu가 39.2%로 가장 높았다. 또한 평균 NDF 함량은 Argus가 65.4%로 낮았고 Kunpu가 69.4%로 가장 높았다. 두 지역에서 티모시의 평균 조단백질 함량은 Alma가 16.5%로 높았으며, Kunpu가 13.9%로 가장 낮았다. 조단백질 수량은 두 지역을 평균하여 대조품종 Climax 보다 높은 수량을 보인 것은 Kunpu의 1,710.5와 Liphlea의 1,536.5kg/ha였다. 대관령에서 오차드그라스의 ADF 함량은Hapsung 20호가 가장 낮았으며(33.4%), 나머지 품종들은 36.2∼37.8% 사이로 비슷한 경향을 보였으며, NDF 함량은 93E가 67.5%로 가장 낮았다. 조단백질 함량은 Hapsung 2호가 가장 높았으며(13.5%), 조단백질 수량도 1,173.2kg/ha으로 가장 높았다. 본 연구결과에 의하면, 고랭지에서 잘 적응하고 생산성이 높은 티모시 품종과 오차드그라스 품종은 각각 Kunpu와 Liphlea 및 Hapsung 2호로 나타났다.

• 한국초지조사료학회지
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• 제38권3호
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• pp.145-149
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• 2018

The objective of this study was to determine the effect of injection application of pig slurry on ammonia ($NH_3$) and nitrous oxide ($N_2O$) emission from timothy (Phleum pretense L.) sward. The three treatments were applied: 1) only water as a control, 2) pig slurry application by broadcasting, 3) pig slurry application by injection. The pig slurry was applied at a rate of $200kg\;N\;ha^{-1}$. Total $NH_3$ and $N_2O$ emission, expressed as a cumulative amount throughout the measurement time (40 days), was $2.68kg\;NH_3-N\;ha^{-1}$ and $6.58g\;N_2O-N\;ha^{-1}$, respectively, in the control. The injection application of pig slurry decreased total $NH_3$ and $N_2O$ emission by 39.8% and 33.3%, respectively, compared to broadcasting application of pig slurry. The present study clearly showed that injection application exhibited positive roles in reducing N losses through $NH_3$ and $N_2O$ emission.

• Asian-Australasian Journal of Animal Sciences
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• 제10권6호
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• pp.593-598
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• 1997

The changes of tissue structure in timothy and alfalfa during ensiling process with silage additives; lactic acid bacteria, cellulase and formic acid, were observed with a video microscope. Stem samples were obtained from the second internode, and cut to divide into 2 pieces. One piece was for observation of ensiled material and the other was for silage. The latter piece was put into a nylon cloth bag, and ensiled with grass for 50 days in a small experimental silo Lignification of the plant tissues was checked by acid phloroglucinol. Natural silage fermentation resulted in some degradation of less lignified parenchyma in both plant species. However, lignified sclerenchyma and vascular bundles remained intact. The cellulase enhanced the degradation of parenchyma tissue, while the formic acid suppressed the degradation. The effect of lactobacillus was small. The percentage of remained cross sectional area of stem and the loss of NDF and ADF by silage fermentation confirmed the observation. High negative correlations were obtained between the remained area and loss of fibrous components during silage fermentation in both plants, and between the loss of fibrous components and in vitro dry matter digestibility in timothy but not in alfalfa.

• Asian-Australasian Journal of Animal Sciences
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• 제5권1호
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• pp.33-37
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• 1992

Napier grass (Pennisetum purpureum Schum.) harvested at an early growth stage was ensiled with 4% of molasses in a polyethylene bag silo which contained 15 kg of chopped forage each. Dry matter (DM) content of the silage was so low as 14.75%, although chemical quality of the silage was very high. Ratio of ammonia nitrogen to total nitrogen was 6.59%, and the pH value of the silage was 3.79. Nutritive value of the silage was estimated using goats and compared to that of a timothy hay as a reference ration. Feeding level of each rations was adjusted to a level of nitrogen (N) recommendation. DM and N digestibilities of the silage were 65.0 and 54.5%, respectively, but those of the timothy hay were 37.6 and 37.2%. Feeding of the napier grass silage maintained body weight and kept positive N retention. Ammonia N concentration in the rumen fluid in goats fed the napier grass silage increased after feeding, but blood urea concentration was constant. Feeding of the timothy hay did not increase ammonia N concentration in the rumen fluid, but increased blood urea concentration. These facts indicated that the napier grass silage had enough digestible DM and N for maintenance ration to goats.

• 한국유기농업학회지
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• 제27권4호
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• pp.529-540
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• 2019

The present study investigated the effect of forage sources and their inclusion levels in diet on the rumen fermentation of Korea traditional goat. Timothy and alfalfa were used as forage sources. Forages were mixed with concentrate diet in different ratios. The ratios of forage to concentrate diets were varied to 1:9, 5:5 and 9:1. The rumen fluid of goat was gathered from slaughter house. Dry matter digestibility was decreased and methane production was increased as forage levels in diet was increased. When forage sources, timothy and alfalfa, were compared, groups with timothy showed greater methane production than the groups of alfalfa. Molar ratio of produced acetate and valerate were increased when forage level in diet was increased. In the case of propionate and butyrate, they were decreased as elevated forage levels in diet. The result of this study provided a basic information for rumen fermentation of Korean traditional goat and these information could be applied in the development of nutritional and feeding strategy.

• 한국초지조사료학회지
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• 제29권3호
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• pp.245-252
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• 2009

최근들어, 친환경 축산이 중요하게 대두대면서 Silvopastrol system에 대한 관심이 높아져 오고 있다. 초지가 나무와 공존하기 위해선 비음에 강한 목초육종과 혼파조합을 선발하는 것이 필요하다. 이에 본 연구는 준 고랭지 지역에서 차광정도에 따른 목초 혼파조합의 식생변화를 살펴보고 생산량과 품질을 조사하여 비음조건에 가장 적합한 목초의 혼파조합을 선발하기 위해 실시하였다. 차광수준이 높아짐에 따라 모든 혼파조합의 목초수량이 감소하는 결과가 나타났으며, 특히 차광수준이 50와 75%에선 급격히 감소하였다. 혼파초지의 조단백질 함량은 차광수준이 높아짐에 따라 유의적으로 높은 결과를 나타냈으며, 같은 차광수준에서 혼파조합의 조단백질 차이는 관측되지 않았다. NDF 및 ADF 함량은 무차광에 비해 차광구에서 낮은 결과가 나타났으며, 차광수준별로는 유의적인 차이는 보이지 않았다. 비음도가 높아짐에 따라 1번 혼파조합 (orchardgass+tall fescue+Kentucky bluegrass+ladino clover+perennial ryegass+timothy)이 가장 낮은 NDF 및 ADF 함량을 나타냈으며, 이 결과는 차광수준이 75%에서 특히 현저하게 나타났다. 본 연구결과는 비음정도가 높은 산림에 임간초지를 조성할 땐 orchardgrass를 주초종으로 하고 tall fescue, Kentucky bluegrass, ladino clover, perennial ryegrass, timothy 혼파하여 조성하고, 비음정도가 낮은 곳에서는 orchardgrass를 주초종으로 Kentucky bluegrass, ladino clover, timothy를 각각 혼합하여 조성하여 관리하면 산림자원과 함께 초자원이 공존하는 친환경 축산의 길이 모색될 수 있다고 생각된다.

• 한국초지조사료학회지
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• 제28권3호
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• pp.165-170
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• 2008

티모시의 colt 품종을 이용하여 최적 조직배양조건을 확립하기 위하여 성숙종자를 배양함에 있어 첨가되어지는 식물 생장조절 물질의종류와 농도가 배발생 캘러스 유도 및 식물체재분화에 미치는 영향을 조사하였다. 배발생 캘러스 유도시 첨가되는 2,4-D는 3 mg/L 첨가 구에서 78%로 캘러스 유도율이 가장 높았으며, 3 mg/L 2,4-D와 0.1 mg/L의 BA를 첨가하여 배양했을 때 형성된 캘러스는 조직적으로 치밀하며 유백색의 배발생 캘러스가 가장 많이 형성되었다. Type B 캘러스만을 이용하여 식물체재분화에는 1 mg/L 2,4-D와 3 mg/L BA가 첨가된 배지에 캘러스를 배양했을 때 25.3%의 재분화율을 나타내었다. 본 연구를 통하여 확립된 효율적인 재분화 시스템은 분자육종을 통한 신품종 티모시의 개발에 유용하게 이용될 수 있을 것이다.

• 한국초지조사료학회지
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• 제27권2호
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• pp.123-128
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• 2007

본 연구는 제주지역에서 말 방목에 적합한 혼파조합을 선발하기 위해 해발 450m에 위치한 한국마사회 경주마육성목장에서 티모시, 오차드그라슨 페레니얼 라이그라스 화본과 위주 초종으로 혼파 파종하여 2년 간 말(더러브렛)을 방목하면서 목초생산성, 식생변화 및 채식율 변화 등을 구명하기 위해 수행되었다. 연간 총 건물생산량은 1, 2차년도에 관계없이 혼파조합 간 유의적인 차이를 보이지 않았으나 시기별에 따라 생산량 차이를 보였다. 식생은 페레니얼 라이그라스가 봄철 크게 우점현상을 보였으나 여름철에는 하고 현상으로 식생구성이 낮게 나타났다. 방목 첫 해 티모시는 식생비율이 낮았으나, 그 후에는 연중 적절한 식생 구성을 보였다. 채식율은 티모시 주초종에서 높게 나타났다(P<0.05). 식물체 NDF, ADF, Ca 및 P 함량은 처리 간 차이를 보이지 않았다. 본 연구결과 제주지역(해발 400m 내외)애서 티모시 주초종(ha 당 티모시 20, 오차드그리스 5, 페레니얼 라이그라스 5, 화이트클로버 2kg)의 혼파조합은 시기별 생산성과 기호성을 고려할 때 말 방목초지로 활용할 수 있을 것으로 사료된다.